ISSN:
0003-276X
Keywords:
Life and Medical Sciences
;
Cell & Developmental Biology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
A technique for the study of microcirculation in living islets, using quartz rod and cinephotomicrography, is presented, together with results of a study of insular microcirculation. With this technique, conditions are standard, results are reproducible and the findings are recorded permanently. The pancreas, bathed constantly in Locke-Ringer's solution at 37°C, is lifted with the spleen from the body, supported on the tip of Knisely's hollow-tipped fused quartz rod, and the lobules are separated gently. Circulation in the transilluminated islets, which are pale yellow, spherical or ovoid bodies with a distinctive vascular pattern, is studied with the biobjective, binocular microscope. For cinephotomicrography, the camera is aligned over one eyepiece of the microscope and the film is exposed at 64 frames per second, using a 750 watt projection bulb as light source. Direct anastomoses of insular and acinar capillaries are not observed. Blood flow in the islets of the anesthetized mouse is rapid and constant, under these conditions of observation. Intravenous epinephrine and ephedrine cause temporary interruption of blood flow in the islets, whereas nor-epinephrine slows but does not stop the circulation. Pitressin also causes slowing of insular blood flow. Insulin, glucagon, hydrocortisone, glucose, alloxan and diphenylthiocarbazone have no acute effect upon the insular microcirculation. A possible relation between the circulatory effect and the hyperglycemic effect of epinephrine and ephedrine is suggested.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/ar.1091460205
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