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  • 1990-1994
  • 1965-1969  (59)
  • 1960-1964  (39)
  • 1890-1899
  • 1870-1879
  • 1965  (59)
  • 1963  (39)
  • Biochemistry and Biotechnology  (98)
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  • 1990-1994
  • 1965-1969  (59)
  • 1960-1964  (39)
  • 1890-1899
  • 1870-1879
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 6-8 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 10-13 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 435-443 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Many antimicrobial agents are available for commercial use, but only a few are truly sporicidal and can be used as chemosterilizers. The action of ethylene oxide, propylene oxide, and β-propiolactone is well documented. Strong acids, e.g., hydrochloric acid, or alkalis may be used as chemosterilizers but their use is extremely limited. The synthetic phenolics accompanied by boiling were recommended, but heat cannot always be employed. Chlorine and iodine have been used in higher concentrations. The use of 8% formaldehyde or 20% formalin was suggested by Spaulding.17 Other aldehydes possess antimicrobial potency. According to work performed in our laboratories, the sporicidal activity of certain alcoholic, saturated dialdehyde solutions equalled or surpassed that of formaldehyde. These included glyoxal, succinaldehyde, and glutaraldehyde. Aqueous alkaline glutaraldehyde solutions killed the most resistant bacterial spores, e.g., B. subtilis and Cl. tetani, within a few hours (Borick1,2). Glutaraldehyde, a 5-carbon dialdehyde, was shown to be a chemosterilizer when tested by various methods, destroying bacteria, including M. tuberculosis, fungi, and viruses. Acid glutaraldehyde solutions stored at room temperature are highly stable, whereas alkaline solutions show a significant change in pH and a diminution of glutaraldehyde concentration on alkalinization. For this reason, fresh (not over 2 weeks old) alkaline glutaraldehyde solutions can be used as chemosterilizers.
    Additional Material: 1 Ill.
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 3-6 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 54-58 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 245-268 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In order to find effective methods to isolate microorganisms that excrete large amounts of amino acids, a number of mutants of Escherichia coli ATCC 4157 were prepared and characterized. The majority were isolated for resistance to amino acid analogs. The penicillin method was also employed to select potential producer strains devoid of a biosynthetic enzyme in one case and a catabolic enzyme (tryptophanase) in another. Both of these enzymes were suspected of reducing the yield of an excerted amino acid. The identification of amino acid excreting colonies was aided by the use of pigmented auxotrophic test bacteria.Amino acid analog resistance was frequently found to increase amino acid production. In particular, excretion of valine and glutamic acid could be achieved with comparative ease. Conditions of culture were of extreme importance for the amount and kind of amino acid produced. Under certain conditions a norvaline-resistant strain and even better a leucine-requiring derivative of it excreted more than 2 mg./ml. of valine into the culture medium. The acetolactate-forming enzyme of this strain was not repressible by leucine in contrast to the same enzyme in the wild type. Reversal of the growth inhibitory effects of amino acid analogs by amino acids was studied on agar plates. This method gave too complex results to be a simple guide in the selection of analogs suitable for the isolation of a particular type of production mutant.
    Additional Material: 8 Tab.
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  • 7
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 129-132 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure for sterilization of microbiological media with β-propiolactone has been developed. Special attention was paid to the maintenance of mild conditions to enable the treatment of media sensitive to high temperature or low pH. The maximum temperature allowed was 40°C. and automatic neutralization of acid produced during hydrolysis was effected by the use of titration unit. The apparatus could be used for several successive sterilization cycles without disconnection.
    Additional Material: 1 Ill.
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  • 8
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 8-10 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 13-16 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 11
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 21-29 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 335-342 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A laboratory process for the isolation of acid soluble nucleotides from bacteria has been scaled-up. Escherichia coli was grown in 220-l. batches, harvested by centrifugation, and extracted with cold trichloracetic acid. The nucleotides in the extract were separated by adsorption onto a Dowex-1 ion exchange resin column followed by elution with dilute hydrochloric acid and lithium chloride. After concentration of the effluent, the lithium chloride was removed by extraction with organic solvents. The overall yield of nucleotides was 75-80%. This elution system has also been used for the separation of nucleotides from bakers' yeast extracts.
    Additional Material: 2 Ill.
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  • 13
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 387-404 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Evidence for generation of the plateau in oxygen uptake during exertion of carbonaceous BOD by pure cultures was sought by making long term Warburg studies using glucose and phthalic acid as substrates. One organism, tentatively identified as Escherichia intermedia, of the 57 tested, exhibited a plateau. Detailed studies on this organism indicated that generation of the plateau depended upon the type of substrate and to some extent upon the initial cell concentration employed, but not upon substrate concentration. Oxygen utilization during the second stage was accompanied by metabolism of secondary extracellular carbon source(s) produced by the cells during metabolism of the original extracellular carbon source (glucose) in the first phase of O2 uptake. The secondary substrate was registered as volatile acids. Selective inhibition of protein synthesis at various times during oxygen uptake led to the conclusion that the plateau occurred because of the need for an induction period before the secondary substrate could be metabolized. Using this organism, it was also found that a plateau could be generated during endogenous respiration. This secondary O2 utilization was accompained by uptake of organic materials excreted into the medium by the cells during metabolism of endogenous carbon sources.
    Additional Material: 7 Ill.
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  • 14
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A procedure described for the partial purification of suspensions of the organisms of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipitation, and centrifugation through a sucrose barrier. More than 40% of the organisms in the original suspension are recovered in 80% of the original volume, with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedures are included.
    Additional Material: 3 Tab.
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  • 15
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 455-470 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The response of heterogeneous populations to shockloading was studied under a variety of operational conditions using synthetic wastes consisting of glycerol, glucose, and a mixture of glucose and glycerol. In all experiments, the cells were acclimated to glycerol. The addition of glucose as a shockloading at various times during the aeration period caused an immediate cessation of glycerol metabolism, and glycerol was not again actively metabolized until all glucose had been assimilated. Experiments conducted at various initial sludge concentrations showed that this effect was not dependent upon biological solids level. Glycerol metabolism was also blocked when glucose was applied as a shockloading to a system operating under severe nitrogen deficiency, further reenforcing the conclusion that there is a blockage mechanism for catabolic pathways which operates to suppress enzyme function rather than enzyme synthesis. It was also found that considerable amounts of metabolic intermediates or end products were released during glucose metabolism regardless of the presence or absence of glycerol. In systems containing a source of nitrogen, these products were metabolized after dissimilation of glucose. In resting cell suspensions, the metabolic intermediates were not subsequently removed, indicating that their metabolism depended upon the synthesis of new enzymes. Using old cell sludge, concurrent rather than sequential removal of glycerol and glucose was observed. The results using two component (C6—C3) carbon sources were in general the same as for C6—C6 synthetic wastes. The results provide further evidence for the generality of sequential substrate removal, and suggest that the metabolite suppressing catabolism of glycerol lies below the triose level.
    Additional Material: 7 Ill.
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  • 16
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 529-553 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A 1-l. fermentor was designed and tested for use as a tissue-culture vessel. It features a temperature control device, impeller agitation without the necessity of a shaft seal, and a means of measuring, recording, and controlling both pH and oxidation-reduction potential (ORP). Tests have shown the ORP to change fairly rapidly with impeller speed variations under conditions of a continuous carbon dioxide-air overlay. Working with strain L mouse fibroblasts (Earle), cell counts of more than 1.25 ×106 ml., without centrifugation and medium renewal, were achieved, and cell counts were maintained above 1 ×106 for more than 30 hr. With the vessel studied, pH control was ±0.05, the ORP control was ±10 mV. Controlled environments for tissue-cell metabolic studies are entirely feasible with this system.
    Additional Material: 7 Ill.
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  • 17
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 343-354 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The oxygen demand of Penicillium chrysogenum culture was supplied by an automatic aeration control system on the basis of the actual respiration of the cells. The simultaneous use of the automatic aeration apparatus and a turbomixer of great shear stress revealed that some effects of agitation are independent of aeration. These effects of agitation are manifested in two ways: (1) in the increased break down of the cell clumps, thus assuring large free cell surfaces for contact with the substrates; and (2) in the increased mixing effect, which assures a continuous mass transfer towardes the free cell surfaces. These effects of the agitation can improve the yield when the O2 level is maintained near to the saturation value. By means of an automatic aeration control system, the influence of carbon dioxide on the metabolism of hyphae and the production of penicillin was found to be demonstrably inhibitory when the dissolved oxygen was above the critical concentration in the culture liquid. This result is supporting evidence for the assumption that aeration is necessary to biosynthetic processes in two equally important ways: (1) to maintain the oxygen level above the critical value; and (2) to assure ventilation, by which the carbon dioxide level may be kept below its inhibitory critical value.
    Additional Material: 4 Ill.
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  • 18
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 405-415 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The formation of gibberellic acid in single-stage continuous cultures was studied. Stirred fermenters of 3 and 20-l. working volumes were used. Maximum output of gibberellic acid was obtained at a holding time of 200 hr. The rate of formation, calculated on the basis of mycelial nitrogen, was comparable with the highest values reported for batch cultures.
    Additional Material: 3 Ill.
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  • 19
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new type of mixer that utilizes the Bernoulli effect has been found highly useful for providing gentle agitation in suspension cultures of fragile mammalian cells in culture volumes up to 6 l.
    Additional Material: 3 Ill.
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  • 20
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 327-334 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed whereby arginine decarboxy-oxidase (ADO) can be produced by growing Streptomyces griseus in submerged form in 20-l. and 200-l. tank fermentors. A medium containing salts, corn steep, glucose, or starch is used. With addition of 0.02% arginine and with limited aeration, a high yield has been obtained.
    Additional Material: 2 Ill.
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  • 21
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An exploratory study is described on the use of a biphasic culture technique for production of toxin by C. tetani. The biphasic system used was a combination of dextran and poly(ethylene glycol) (PEG) in the presence of a protein-free medium. The system supported good growth and gave a favorable phase ratio (top/bottom) of 15:1. The majority of the bacterial cells were in the bottom dextran phase, while the greater part of the toxin was in the upper PEG-medium phase. Toxin yields from a typical experiment were 109 mouse IP MLD/ml. of upper phase filtrate. The biphasic culture system was also adaptable to continuous culture, with approximately similar toxin levels found in the upper phase. The appearance of the clostridial cells in older cultures suggested the possibility of protoplast formation, perhaps stabilized by the polymers present. In vitro toxin titration (Lf) was inhibited by the presence of the polymers. Bacillus anthracis also grew well in the biphasic system and elaborated protective antigen, primarily into the upper phase.
    Additional Material: 6 Ill.
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  • 22
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
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  • 23
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 471-490 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The growth of Thiobacillus Thiooxidans, utilizing sulfur in three media, was studied by observing changes in halfcell emf, bacterial cell count, and acid production, all as a function of time. A comparison of the biological halfcell emf with comparable control halfcells reveals that T. thiooxidans makes an electrochemical contribution to halfcell voltage. A change from the more complex medium of Skerman's mineral salts to ATCC allowed a clearer delineation of the ability of T. thiooxidans to make an electrochemical contribution. Reproducible biological halfcell emf's were obtained when the ferrous sulfate was removed from the ATCC medium. One halfcell, consisting of T. thiooxidans utilizing sulfur in ATCC, was observed over a 111-day period. During this time, the initial halfcell voltage of -0.35 V. decreased to a value of -0.64 V. (hydrogen emf series). T. thiooxidans, in utilizing sulfur, produces only sulfate ion, thereby simplifying the identification of an electrochemical contribution during growth.
    Additional Material: 12 Ill.
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  • 24
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 491-506 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The electrochemical activity of the individual chemicals in the nutrient medium for growth of Thiobacillus thiooxidans was studied along with the effect of the gases in equilibrium with their solutions. Several chemicals were active individually; however, the magnitudes, as measured by changes in half-cell potential, were less than that observed when T. thiooxidans was present in their composite mixture. Sterilized Skerman's mineral salts and the American Type Culture Collection (without ferrous sulfate) media were not sensitive electrochemically to changes from pure oxygen to nitrogen atmospheres. When T. thiooxidans was present in these media, the biological half-cell emf became sensitive to changes in the oxygen content of the atmosphere in equilibrium with the organism and nutrient. The ability of T. thiooxidans to make an electrochemical contribution, as registered by a platinum electrode, is substantiated further by these investigations.
    Additional Material: 8 Ill.
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  • 25
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 72-74 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 26
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 74-75 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 27
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 70-72 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 28
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 29-51 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 29
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 60-65 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 30
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 171-175 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is described for the isolation of bacterial polysaccharide B-1459 from aqueous dispersion as a calcium hydroxide complex. The polysaccharide is liberated in fibrous form from the complex by means of hydrochloric acid in methanol solution. Overall recovery of the polysaccharide from aqueous dispersion is 85%. Direct conversion of the insoluble calcium hydroxide complex to aqueous polysaccharide solution appears to be a low-cost procedure for preparing viscous solutions for industrial use.
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  • 31
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    Biotechnology and Bioengineering 7 (1965), S. 215-227 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of operating temperature upon relative stripping of a two component (ethanol-water) mixture by dry air, with the aid of a previously derived equation,1 is considered. The variations encountered in the relative humidity of air sparged to fermentors are discussed. A general equation is developed for the stripping of a more volatile component from a solution in water by wet air. The theoretical results derived from this equation are tested experimentally at one particular level of prehumidity of the air sparged to an ethanol-water system.
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  • 32
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    Biotechnology and Bioengineering 7 (1965), S. 279-283 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Continuous cultures of some heterotrophic Spirilli (Sp. serpens, Sp. curvatum, Sp. sp.) exhibit threshold concentrations of lactate, the growth limiting carbon and energy source, around 15 mg./l. This value corresponds to a threshold population density of 0.92 mg. dry weight/l. at a dilution rate of D = 0.185 hr.-1 (= 0.5 maximum growth rate). The minimum population density, which is regarded as equivalent to the starter population in batch culture, was dependent on the growth rate and the redox potential. The existence of minimum populations and the pronounced effect of population density on growth at low nutrient levels seem to be widespread phenomena closely related to growth under suboptimal conditions.
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  • 33
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    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 7 (1965), S. 322-325 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 34
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    Biotechnology and Bioengineering 7 (1965), S. 309-319 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Pseudomonas aeruginosa NCTC 5940 was grown in an aerated mineral salts medium with pure octadecane as sole carbon source, both in batch and continuous culture experiments. Carbon and nitrogen balances were made under different conditions. It was found that the only significant products were fresh cell tissue and carbon dioxide, although traces of several fatty acids were detected.
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  • 35
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    Biotechnology and Bioengineering 7 (1965), S. 321-322 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 36
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    Biotechnology and Bioengineering 7 (1965) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 37
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    Biotechnology and Bioengineering 7 (1965), S. 16-19 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 38
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    Biotechnology and Bioengineering 7 (1965), S. 51-54 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 39
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    Biotechnology and Bioengineering 7 (1965), S. 65-69 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 40
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    Biotechnology and Bioengineering 7 (1965), S. 161-169 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method to make purified polysaccharide synthesized exocellularly from cereal carbohydrates by the bacterium Xanthomonas campestris NRRL B-1459 and a characterization of its properties have been reported previously by this Laboratory. Because of increasing commercial interest in a less purified polymer, we have extended our work to include production of a lower cost industrial-grade product. Fermentation of media containing 2.25% corn sugar, seeded with 5% inoculum of X. campestris NRRL B-1459 and cultured aerobically at 82°F., is complete in 96 hr. The viscous fermented broth contains about 1.5% polymer. A tan product containing approximately 60% polymer and having good viscosity characteristics is obtained by drum drying the fermented broth at steam pressures below 40 p.s.i.g, 287°F. Drying at higher temperatures adversely affects viscosity. A good product is also obtained by spray drying. Cost estimates indicate that 5 million lb./yr. of dried crude solids could be produced for 37 cents/lb. by drum drying or for 41 cents a pound by spray drying.
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  • 41
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    Biotechnology and Bioengineering 7 (1965), S. 199-214 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A theoretical approach to the prediction of stripping or accumulation of minor components in solution with water by a sparged stream of dry air bubbles is developed and tested experimentally for two model systems, containing 1 mole-% respectively, of ethanol, to represent situations where the minor component is more volatile than water, and of acetic acid, to represent the reverse. Close agreement between theory and practice is achieved in the former case, where an overall reduction in the liquid phase concentration of the minor component occurs, while in the latter, where overall accumulation occurs, there is some discrepancy. Considerations for an extension of the study are included.
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  • 42
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    Biotechnology and Bioengineering 7 (1965), S. 269-278 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The addition of potassium ferrocyanide to the molasses substrate (mash) used in the citric acid fermentation had little or no effect on the total carbon, nitrogen, or phosphorus content of the substrate but reduced the ash content by 1-4% depending on the type and crop year of molasses. Eighteen of the 21 metals identified in beet molasses by spectrographic analysis were precipitated in part by the treatment. The metals known to interfere with citric acid production, particularly manganese and iron, were those most efficiently precipitated. At mash temperatures of 80°C. and approximately 100°C., precipitation was completed within 15 min. after ferrocyanide addition and the reductions in the soluble mineral content in each case were similar. At 25°C. the precipitation reactions occurred but required more than 50 hr. to complete. Significantly more total mineral and more iron, calcium, and copper were precipitated at pHs 4 and 6 than at pH 8; manganese precipitation, however, was not affected by these pH changes.
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  • 43
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    Biotechnology and Bioengineering 7 (1965), S. 101-128 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bei der kontinuierlichen Züchtung von Mikroorganismen ist die zuverlässige Aufrechterhaltung des stationären Zustandes die Grundbedingung für die erfolgreiche Untersuchung der biologischen Probleme. Auftretende Instabilitäten können apparativen oder biologischen Ursprunges sein; eine einwanddfrei funktionierende Ausrüstung ist daher bei der Einführung der Kontinuität primäres Erfordernis. Es wird auf die wichtigsten Ursachen, welche zu Instabilitäten führen können, eingegangen und eine Laborapparatur beschrieben, welche eine Basisausrüstung umfasst, die speziell für hohe Betriebssicherheit und Einfachheit der Bedienung ausgelegt wurde. Besondere Aufmerksamkeit wurde der Konstanz des Zuflusses f des Mediums, und des arbeitenden Volumens v, sowie der sicheren Ausschaltung von Infektionen durch Verwendung sterilisierbarer pH-Elektroden und eines Membranverschlusses geschenkt.
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  • 44
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    Biotechnology and Bioengineering 7 (1965), S. 555-558 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Additional Material: 2 Ill.
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  • 45
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    Biotechnology and Bioengineering 7 (1965), S. 59-60 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 46
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    Biotechnology and Bioengineering 7 (1965), S. 139-159 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A small fraction, about 0.05%, of the microorganisms in milk are resistant to pressure to at least 150,000 p.s.i. The pressure-resistant microorganisms were shown to be mainly bacterial spores. Some of the pressure-resistant organisms were identified. The lethal effect of pressure is more pronounced in the solid phase than in the liquid phase of water. B. subtilis was found to be capable of surviving solid-phase transitions from Ice II, III, and V to Ice I. The lethal effect of high pressure in the liquid phase of water was found not to be counteracted by an increase in temperature. The pressure resistance of B. subtilis was found to be maximum around neutral or slightly basic pH. The damaging effect of low and high pH are counteracted by sodium chloride and glucose. It is suggested that the primary mechanism of protein denaturation by pressures above 30,000 p.s.i. is ionization and formation of ionic bonds between charged groups on proteins, altering their solubility.
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  • 47
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    Biotechnology and Bioengineering 7 (1965), S. 177-198 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Steam corrosion and steam corrosion inhibition of dental and surgical instrument materials during autoclave sterilization have been investigated. The corrosion tests were performed on carbon steel test samples and on round dental burrs. Cyclohexylamine or decylamine were used as corrosion inhibitors, added to the “feed water,” i.e., deionized water, to a concentration of 0.1%. Before the corrosion experiment the surfaces of the test materials were standardized and calibrated by means of ultrasonic treatment. After the experiment the corrosion products were removed from the test samples or burrs using the same ultrasonic cleaning. Deionized water was used as cleaning liquid, and was then analyzed for the amount of Fe dissolved. The amount of corrosion was expressed as μg. Fe/cm.2 sample surface area or μg. Fe/burr. The results show that considerable corrosion occurs when carbon steels are exposed to steam during autoclaving, but if cyclohexylamine or decylamine is added to the steam under the otherwise unchanged experimental conditions the total corrosion was reduced by 96-99%. No cytotoxic effect on HeLa cells could be detected with surface products possibly containing traces of amine and/or amine salts - nor with corrosion products alone - when these products were removed from the test materials by means of ultrasonic cleaning.
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  • 48
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    Biotechnology and Bioengineering 7 (1965), S. 517-528 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A substrain of Bacillus cereus 569/H produced above 10,000 units of penicillinase/ ml. when grown in a pilot-plant fermentor using a medium containing Casamino acids techn. (Difco) or N-Z-Amine type B (Sheffield), and salts. Simplified purification and concentration procedures give an overall yield of 50-65% enzyme. The freeze-dried enzyme preparation had a good storage stability in vacuumsealed ampules kept at 4, 30, and 37°C. In vials containing air in the head space, partial inactivation occurred in two months at 30 and 37°C. The freezedried preparation showed satisfactory performance in the production of yoghurt fermented milk.
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  • 49
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    Biotechnology and Bioengineering 7 (1965), S. 76-85 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 50
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    Biotechnology and Bioengineering 7 (1965), S. 85-90 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 51
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    Biotechnology and Bioengineering 7 (1965), S. 91-100 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An automatic aeration controller was constructed to assure the optimal amount of oxygen for aerobic biosynthetic processes. A platinum electrode and a KCl bridge for the calomel electrode were inserted hermetically into the fermentor and were sterilized with the culture medium. The electrode potential developed at the surface of the Pt electrode continuously indicated the actual oxygen concentration of the medium. This served as the controlled variable of the system. The concentration of dissolved oxygen resulted from the actual demand and supply of the microorganism. The electrode potential, which was the measured variable of the process, operated a closed-loop automatic aeration system. The controller activated an air inlet valve in order to balance the controlled variable. The automatic controller operated in fermentors of industrial scale for long periods in a satisfactory manner.
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  • 52
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    Biotechnology and Bioengineering 7 (1965), S. 133-137 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In a study of fermentation methods of producing amphotericin B-14C, a variety of C-14 labeled precursors were added to cultures of the amphotericin-B producing streptomycete growing in a glucose-cottonseed meal medium. Acetate-14C and propionate-14C were efficiently incorporated into the amphotericin-B while formate-14C, glucose-U-14C and methionine-14CH3 were not.
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  • 53
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    Biotechnology and Bioengineering 7 (1965) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 54
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    Biotechnology and Bioengineering 7 (1965), S. 229-243 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The kinetics of the gluconic acid fermentation have been studied at pH 5.8 Pseudomonas ovalis B-1486 was used as the fermenting organism. D-Gluconolactone was found as an intermediate in the fermentation and accumulated at times in large amounts. When this occurred, hydrolysis of the lactone was found to be the rate limiting reaction. This can be, under certain conditions, a poor fermentation in which to study oxygen transfer limiting conditions. Further, it is impossible to relate batch and continuous kinetic data for this fermentation unless the lactone hydrolysis reaction is taken into account.
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  • 55
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    Biotechnology and Bioengineering 7 (1965), S. 285-293 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With a view to preparing substitutes for liver or other animal protein hydrolysates used in the treatment of protein malnutrition, a process has been developed based on sesame and mustard cakes as the starting materials. The laboratory process has been successfully scaled up to pilot plant production to compute tentative costing schedule. Essentially the process consists in defatting the cake, isolating the protein by peptization and isoelectric precipitation, enzymatically digesting the protein isolate by papain, and concentrating the digest under reduced pressure. The final product is a light brown fluffy powder, rich in almost all the essential amino acids. Growth experiments with rats have indicated that the product is comparable to commercial casein although supplementation with lysine could further enhance its biological value.
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  • 56
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    Biotechnology and Bioengineering 7 (1965), S. 295-308 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Large cultures of microscopic, unicellular algae have been proposed as a means of maintaining a life-supporting atmosphere in a closed, manned system. To achieve vigorous growth of the algal culture it is necessary to subject individual algae alternately to short periods of high intensity light and darkness. One of the means suggested for obtaining a favorable light-dark sequence for photosynthesis is to cause turbulence in a closed channel on which light is incident. Since light is rapidly attenuated in a dense suspension, there will be illuminated regions adjacent to the channel walls and a dark central core. The random motions of turbulence normal to the direction of flow would move the algae alternately from the illuminated regions to the dark region and back again. This paper indicates a method for analyzing the motion of algae into and out of the illuminated region of a channel formed by flat, parallel, transparent plates, with light incident on the plates. Matching of a probability model with a diffusion model makes it possible to estimate the light-dark sequence which could be achieved by turbulence. The results indicate that favorable sequences by this mechanism are unlikely.
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  • 57
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    Biotechnology and Bioengineering 7 (1965), S. 367-386 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The construction of a “Porton type” chemostat of 8-l. working volume, based on the New Brunswick Scientific Co., Inc., Model F-14 Fermentor, is detailed. Instrumentation for temperature, pH, and foam control, and for medium supply are described and operational problems discussed.
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  • 58
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    Biotechnology and Bioengineering 7 (1965), S. 445-446 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 59
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    Biotechnology and Bioengineering 7 (1965), S. 417-434 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This paper describes the design and operation of an industrial type culture system for the submerged growth of BHK 21 cells at a volume of 30 l. Using a modified Eagle's medium and with the culture controlled at pH 7.4 ± 0.05 and at a temperature of 35 ± 0.25°C., the cells grew from 0.5 × 106 to about 2.5 × 106 viable cells/ml. in 50 hr. at stirrer speeds of 330 and 460 rpm. The significance of certain aspects of design and operation of a culture system of this type is discussed.
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  • 60
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 61
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    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
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  • 62
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    Biotechnology and Bioengineering 5 (1963) 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 63
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A 50-gal. growth chamber is described together with its air filter and humidifier. The unit was designed especially for the production of Neurospora crassa, and was found to be effective in producing bulk amounts of mycelia with a high specific activity of tryptophan synthetase.
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  • 64
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    Biotechnology and Bioengineering 5 (1963), S. 87-89 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Addition of the S-2-hydroxyethyl-, S-2-chloroethyl-, S-n-propyl-, and S-iso-propyl-derivatives of homocysteine, and methoxinine to S. aureofaciens (ATCC 13900) fermentations resulted in production of 7-chloro-6-demethyltetracycline in addition to the normally synthesized 7-chlortetracycline.
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  • 65
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    Biotechnology and Bioengineering 5 (1963), S. 123-129 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm.2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm.2 areas renged from 8 to 12% between tests of 216 counts each.
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  • 66
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    Biotechnology and Bioengineering 5 (1963) 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 67
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    Biotechnology and Bioengineering 5 (1963), S. 185-192 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: By growing Aspergillus parasiticus (C.M.I. 15957) under controlled conditions on sterilized peanuts an average of 265 mg. of aflatoxin per kilogram of peanuts has been produced and subsequently isolated. Other strains from the A. flavus-oryzae group gave lower yields of aflatoxin and one such strain gave aflatoxin from which certain normal components were absent. The aflatoxin produced on sterilized peanuts by any particular strain of A. flavus-oryzae was shown by thin-layer chromatography to contain the same major components as were produced by that strain on unsterilized whole peanuts.
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  • 68
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    Biotechnology and Bioengineering 5 (1963), S. 221-230 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A process has been developed giving over-all yields of about 50%: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Dowex 1 ion-exchange resin, and groups of nucleotides are removed by pH-defined eluents and recovered by a second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual cells.
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  • 69
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    Biotechnology and Bioengineering 5 (1963), S. 231-242 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the present paper the authors described laboratory fermentors used in the Antibiotics Research Institute since 1958 for research concerning the technology of the biosynthesis of antibiotics and amino acids and other aerobic fermentations. The lids of the fermentors are made from stainless steel, the vessels are made from glass. The fermentors are placed, always four each, in eight water baths with automatic temperature regulation within a range of ±0.5°C. Details regarding construction, auxiliary equipment, and working procedures used for the sterilization of fermentors and media, inoculation, injection of additives, and defoaming are given. The set-up for one-stage and multi-stage, batch and continuous cultivation are discussed.
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  • 70
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    Biotechnology and Bioengineering 5 (1963) 
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    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 71
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    Biotechnology and Bioengineering 5 (1963), S. 131-146 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A sensitive cup plate assay method for lysine on large plates using S. faecalis ATCC 6057 as the test organism is described in detail. Well-defined zones of dense growth were obtained in a rectilinear dose - response range from 5 to 3000 μg. of L-lysine/ml. Comparative assays on twelve different samples were carried out using the turbidimetric tube assay method with Leuc. mesenteroides ATCC 8042 and the cup plate method with S. faecalis ATCC 6057. Good agreement between the two assay methods was obtained for five samples. For the remaining seven samples the tube assay values were appreciably higher than those obtained by the plate method. This discrepancy is discussed. Pure lysine added to various sample extracts could be quantitatively recovered. The specificity of the lysine requirement of S. faecalis ATCC 6057 in the plate assay was also investigated. Under certain experimental conditions, a weak and variable response was obtained with D-lysine and the polyamines cadaverine, spermine, and spermidine. There was generally no difficulty in distinguishing the D-lysine and polyamine growth zones from the L-lysine growth zones. This growth effect of D-lysine and polyamines could be diminished and even completely abolished by increasing the DL-hydroxylysine hydrochloride level in the plate medium. The lysine peptides glycyl-L-lysine and L-lysylglycine gave excellent growth zones of the same appearance as those produced by L-lysine. The activity of these two peptides in cup tests were on a molar basis about 70% of that of L-lysine.
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  • 72
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    Biotechnology and Bioengineering 5 (1963), S. 9-15 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method by which a paramagnetic oxygen analyzer is used to determine oxygen consumption and carbon dioxide production during aerobic fermentation of Torulopsis utilis is described. The volume of carbon dioxide produced is not necessarily related to the volume of oxygen consumed by the microorganisms. For this reason, all carbon dioxide is removed from the effluent gas before it enters the analyzer when determining oxygen consumption. A method for calibrating the analyzer to correct for this removal is outlined. When determining carbon dioxide, effluent gas bypasses the carbon dioxide absorption tower before entering the analyzer. The carbon dioxide which it contains dilutes the oxygen remaining in the gas. The magnitude of this dilution can be used to calculate the percentage of carbon dioxide produced in terms of the air flow to the fermentor. Equipment required to alternately measure and record oxygen consumption and carbon dioxide production is described.
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  • 73
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    Biotechnology and Bioengineering 5 (1963), S. 21-25 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Cobamide production by Butyribacterium rettgeri Barker and Haas (ATCC 10825) was studied on a laboratory scale. Yields of the order of 5 μg./ml. were obtained when this anaerobe was grown for 6 days at 37°C. in a medium containing glucose, cornsteep liquor, cobalt nitrate, calcium carbonate, and sufficient ammonium hydroxide to keep the pH between 6.0 and 7.2. All of the vitamin was found associated with the bacterial cells, and examination of extracts of these cells showed the presence of 5,6-dimethylbenzimidazolylcobamide coenzyme in large quantities. Supplementation of the fermentation medium with a number of benzimidazoles resulted in formation of traces of “new” cobamides with these bases in the nucleotide protion of the cobamide. Supplementation with a number of purines did not result in incorporation of these moieties in the cobamide portion of the molecule.
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  • 74
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    Biotechnology and Bioengineering 5 (1963), S. 49-52 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An investigation was made of the stability of product limited continuous cultures of Saccharomyces carlsbergensis and Pseudomonas ovalis. No oscillations were found in pH or glucose concentration during the steady state, and no overshoot resulted from changing from one steady state to another.
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  • 75
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    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
    Additional Material: 3 Ill.
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  • 76
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    Biotechnology and Bioengineering 5 (1963), S. 109-121 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbiological production of beta-carotene previously investigated in shakenflask culture has been scaled-up to 20-liter fermentors containing an operating volume of 10-liters. Yields obtained in shaken flasks, approximately 100 mg./100 ml. have been reproduced with a fermentation time of 72 hr. Factors affecting the fermentation: inoculum, mash ingredients, and environmental conditions, were investigated. Typical data are presented. A preliminary cost analysis indicates a “cost to make” of $31.35/kg. of carotene contained in the dried fermentation solids.
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  • 77
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    Biotechnology and Bioengineering 5 (1963), S. 167-184 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Shock pressure waves with a frequency of 50/sec. and a maximal pressure of 60-70 kg./cm.2 greatly reduced the viability of E. coli. A similar effect was obtained also on staphylococci, enterococci, Salmonella Zanzibar, and coliphages T2. On the contrary no effect on spores of B. subtilis was found. The effect was practically independent of the maximal pressure within the range from 30-80 kg./cm.2, the highest pressure tested. The effect decreased with increasing cell density of the bacterial suspension. Bacteria surviving shock treatment died considerably more rapidly when stored at 4°C. than bacteria treated in the same way except for exposure to pressure waves. Morphological examinations with the aid of ordinary light microscopy as well as electron microscopy suggest that the effect was due to mechanical action on the bacterial cell walls.
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  • 78
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    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
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  • 79
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    Biotechnology and Bioengineering 5 (1963), S. 287-307 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Sphaerotilus is usually present in biological waste treatment processes and is considered to be a nuisance organism because it may cause bulking of activated sludge. However, it may play an important role in the decomposition of putrescible organic matter in properly operated biological waste treatment processes. The research described herein is a study of the variety of types of organic compounds which may be decomposed by Sphaerotilus and the effect of some environmental factors upon the rate of decomposition. Although there are some types of compounds which cannot be decomposed by Sphaerotilus, it is shown that this organism is able to assimilate and oxidize a large variety of organic compounds and that it is tolerant of an extremely wide range of environmental conditions. The role of Sphaerotilus in biological waste treatment is discussed in the light of the data presented.
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  • 80
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    Biotechnology and Bioengineering 5 (1963), S. 355-365 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It has been found that a bacterium (isolant C12B) grew poorly on 6 and 8 carbon alcohols, well on 10 and 12 carbon alcohols, and slowly but well on the 14 to 18 carbon compounds. Unexpectedly, resting cells oxidized the 6 and 8 carbon compounds most readily and cell-free extracts oxidized 6, 8, and 10 carbon alcohols. Extracts from cells adapted to sodium lauryl sulfate oxidized the 12 carbon alcohol as well, if small concentrations of substrate were used. The bacterium grew on benzene sulfonate and adapted resting cells oxidized that compound. Pure culture degradation studies revealed that isolant C12B used only 11% of tetrapropylene ABS supplied as a sole carbon source, whereas 66% of sodium lauryl sulfate was degraded. Intermediate quantities of kerosene-based alkyl benzene sulfonate and a tallow-derived detergent were utilized. Extracts of another bacterium, TEG-5, grown on tetraethylene glycol, contained enzymes which oxidized polyethylene glycols up to an average molecular weight of 600, but not any larger polymers. This oxidation was visualized by coupling to reduction of ferricyanide.
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  • 81
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    Biotechnology and Bioengineering 5 (1963), S. 17-20 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The applicability of physicochemical adsorption laws to the sorption of methylene blue by dead Penicillium chrysogenum was studied. The specific area (area/mass) of the mold was measured.
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  • 82
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    Biotechnology and Bioengineering 5 (1963), S. 147-165 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of factors was found to affect the cup plate assay for lysine with Streptococcus faecalis ATCC 6057. Hydroxylysine, aspartic acid, and Tween 80 were found to be among the essential components of the assay medium. Removal of any of these three components produced diffuse growth zones or otherwise unsatisfactory plates. The inclusion of asparagine and additional dipotassium phosphate to the medium was found to be advantageous but not an absolute requirement. It was important not to autoclave the hydroxylysine and the additional dipotassium phosphate together with the rest of the assay medium, but to add these components to the sterilized medium immediately before pouring the plates. With certain concentrations of hydroxylysine in the medium, the addition of L-glutamine was found to potentiate the effect of hydroxylysine and thereby improve the lysine plates. The effect of graded amounts of the above mentioned medium components and of the pH of the assay medium was also studied. Increasing the sterilization time of the medium from 5 to 10 min. at 120°C. improved the readability of the lysine plates and led to more easily reproducible results. Factors influencing the preparation of the inoculum for the lysine plates were also studied: the age of the stab culture, the amount of inoculum used per plate, and the influence of the nature of the suspension medium used for washing the inoculum.
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  • 83
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    Biotechnology and Bioengineering 5 (1963), S. 193-199 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Ferrocyanide-treated beet molasses was fermented in fully baffled mechanically agitated, 2.5-liter, tank-type fermenters using pellets of Aspergillus niger grown in shake flasks as an inoculum. The object of the study was to adapt the fermentation method previously developed in an unstirred tower-type fermenter to a conventional tank-type fermenter. Under selected conditions of stirring (agitator speed 400-700 rpm) and sparging (medium porosity sintered disk), high yields of citric acid (8.6% citric acid, 70% conversion of available sugar) were obtained in 140 hr. of fermentation. There was little relation between citric acid yield and aeration conditions in the fermenter as judged by mash viscosity measurements and sulfite oxidation studies.
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  • 84
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    Biotechnology and Bioengineering 5 (1963), S. 53-58 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ-free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the cabinet is used to blow sterile air into the cabinet in sufficient quantity so as to flow out of the hand ports at a velocity of 50 ft./min. In the design, careful consideration was given to the ease of manipulations in the cabinet.
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  • 85
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Removal of the pungent factor, allyl isothiocyanate, will partly determine whether mustard seed can become a commerical source for oil and meal in the United States. In processing studies at the Northern Laboratory, the mustard glucoside was converted enzymatically and the pungent oil was removed. This process has now been extended to pilot-plant scale by using filtration-extraction equipment at the Southern Laboratory. After desolventization and further steam stripping, the extracted meal had a residual content of 2.9% crude fat and 0.004% allyl isothiocyanate.
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  • 86
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    Biotechnology and Bioengineering 5 (1963), S. 59-74 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The distribution of dissolved oxygen in agitated, sparger-aerated aqueous solutions has been investigated by polarographic techniques. The results of the investigations are discussed in relation to earlier studies in bubble distribution in such systems. Liquid-phase mixing is good at agitation intensities well below that necessary to disperse bubbles throughout the liquid.Suspended solids interfere with the transfer of oxygen unless they are freely suspended. Discrete fibers (paper-pulp) are much easier to suspend than molds in either filamentous or pellet forms. Mass-transfer at the cell-liquid interface appears to be unaffected, in both bacterial and mold suspensions, by the intensity of agitation, provided that the organisms are freely suspended.
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  • 87
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    Biotechnology and Bioengineering 5 (1963), S. 83-85 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The inclusion of ethionine in Streptomyces rimosus fermentation resulted in the production of N-methylethyloxytetracycline. The compound has been isolated, crystallized, and a number of its chemical properties determined.
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  • 88
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    Biotechnology and Bioengineering 5 (1963), S. 101-107 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An adsorption colorimetric method is proposed for the determination of the percentage of dead cells in suspensions of Penicillium chrysogenum, based on the observed fact that a linear relation exists between that percentage and the dye concentration at the equilibrium point.
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  • 89
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    Biotechnology and Bioengineering 5 (1963), S. 91-99 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Microbial polysaccharides which have many potential industrial applications are receiving more and more attention. One of the economic obstacles to the commercialization of these polysaccharides has been the cost of isolating them from fermented broth. To reduce this cost a recycling process was developed. The polyanionic polysaccharide synthesized from glucose by the activity of the bacterium Xanthomonas campestris NRRL B-1459 is precipitated from the fermented broth with a quaternary ammonium compound (QAC).QAC is removed from the precipitate with methanol leaving the water-soluble polysaccharide. Evaporation of the methanol wash removed the methanol and substantially all the QAC was recovered in the concentrate. This was recycled for subsequent precipitations. Chemical assay and the viscosity of water solutions of the isolated polysaccharide indicate no adverse effects on it from recycled QAC. The cost to make this polysaccharide by the described procedure is estimated to be $1.14/lb. This cost estimate includes land, buildings, raw materials, equipment, labor and supervision, utilities, factory supplies, working capital, and plant overhead.
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  • 90
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    Biotechnology and Bioengineering 5 (1963), S. 211-220 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A disposable plastic tetrahedron bag is described which is suitable for holding a 50 ml. culture on a shaker table. Bags can be made out of polypropylene or Teflon and they are quite simple and inexpensive. The tetrahedrons make excellent use of the shaker table space and they give a threefold increase in aeration efficiency over Erlenmeyer flasks.
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  • 91
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    Biotechnology and Bioengineering 5 (1963), S. 243-251 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The metabolic pathway of degradation of naphthalene to salicylic acid has been studied by several investigators. The rates of conversion and of cell growth are the basis for the present study. In the early phase of the fermentation, the salicylic acid accumulation followed an exponential curve, as did the bacterial cell accumulation. In the second phase, most of the salicylic acid was produced according to a linear or zero reaction, at rates of 0.3 to 0.5 g./l./hr. A linear accumulation of microbial cell weight accompanied the linear salicylic acid formation. Despite the correlation of growth and salicylic acid production, the type or amount of nitrogen supplied for growth had only a small effect on yield over broad limits. The optimum pH for salicylic acid accumulation was approximately 6.5. Below this value a slower rate of production and lower final yield was seen. Above pH 6.5, a rapid degradation of salicylic acid was observed. A temperature range from 25 to 30°C. was optimum. At 35°C., no growth occurred, while at 20°C. very slow salicylic acid accumulation resulted.
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  • 92
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    Biotechnology and Bioengineering 5 (1963), S. 255-273 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Stabilization ponds have been widely accepted in recent years, following studies that established deeper understanding of the phenomena associated with the stabilization process. A compilation of information on the use of both sewage and industrial ponds is presented showing types and geographical location in the United States. A total of 1,304 stabilization ponds currently are used as the principal method of sewage treatment serving a population of 2,138,085. Ponds are used for tertiary treatment serving a population of 2,146,951, in 343 instances. Use of 827 industrial ponds by 31 industries in 44 States is also indicated. Indiana ranks first in usage with 159 ponds and Callfornia second with 140. Ponds were classed as effective by 66 percent of the 455 users who reported on this item. Of 149 reports on odors, 73% indicated nuisances. Insect problems were reported in only a small number of instances. Data are presented on design considerations and the mechanism of waste stabilization ponds. Aerobic ponds are given greatest emphasis. Photosynthesis and its dependence upon the algal mass, suitable temperature, incident light penetration, nutrient supply, and induced vertical mixing by wind are of prime importance in the stabilization mechanism. Odors are associated with prolonged anaerobic conditions, and these may persist up to 4 weeks following extended ice cover in cold climates, if BOD loadings are 25 lbs. per acre per day or greater. Nitrogen and carbon may be limiting factors in the development of an algal mass. Coliform, BOD, and nutrient removals are presented for various physical, chemical, and climatic conditions. Although fecal streptococci removal averaged 97% in a study pond, they were still discharged in the effluent at a rate of 700,000 per second in winter and 70,000 per second in summer. A striking similarity exists generally among the algal speciation in stabilization ponds, regardless of geographic location. The algal mass is, however, dependent upon unique pond conditions and location, and may vary upwards to nearly 5 million algal cells per milliliter, 34,000 p.p.m. by volume, or 30-35 tons per acre per year. The problem of blue-green algal mats and resultant pigpen odors in extremely localized areas is duscussed. Midges and midge larvae may have an impact on the nutrient cycle in a stabilization pond, and may pose a potential localized nuisance in some areas. Mosquito problems have usually been associated with growth of aquatic vegetation. Suggestions for minimizing the potential problem are discussed.
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  • 93
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    Biotechnology and Bioengineering 5 (1963), S. 201-209 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: It is a well-known fact that choosing a sequence of optimal control variables in each reactor tank of a multistage reactor system can result in a considerable increase of the desired product for a consecutive reaction system. The discrete maximum principle is applied to a generalized optimization problem of first order consecutive biochemical reaction system. A detailed numerical solution as an example is also presented for illustration.
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  • 94
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    Biotechnology and Bioengineering 5 (1963), S. 275-285 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Phenol-bearing waste waters continue to be an area of wide concern for various industries faced with the task of cleaning up effluents containing relatively small amounts of these materials. The microbiological aspects of the degradation of these materials in pure culture and waste treatment plants is reviewed. Actual operating experiences at three plants of The Dow Chemical Company, where phenols are present in combination with other widely varying waste constituents, are discussed.
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  • 95
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    Biotechnology and Bioengineering 5 (1963), S. 331-345 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: These experiments show that ABS can be adsorbed and desorbed from microbial cell surfaces. The adsorption equilibria between the free and adsorbed phases are primarily influenced by the pH of the system. The effects of ABS upon the biochemical activities of bacteria depended upon the amount of ABS adsorbed and the type of bacteria present. The behaviour of ABS is essentially the same whether in the presence of pure or mixed bacterial cultures. Two desorption experiments with soil from a reclaimed water spreading basin which has received sewage over a six month period showed that at least 82% of the ABS “loss” was recoverable from the soil. In all the experiments carried out in this study no evidence was obtained for the biochemical degradation of ABS.
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  • 96
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    Biotechnology and Bioengineering 5 (1963), S. 347-354 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the emphasis being given to the development of biologically soft detergents, it is necessary to establish eriteria defining biodegradability of these materials. It is the authors' opinion that a biologically soft detergent should have the following characteristics: (1) Under normal operations the material should be 85% removable by activated sludge. (2) Normally high discharges of the detergent should not influence biological activity. (3) The detergent should ultimately degrade to carbon dioxide and water.
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  • 97
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    Biotechnology and Bioengineering 5 (1963), S. 367-384 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Because branched-chain alkyl benzene sulfonate (ABS) is well known to create water pollution problems in surface water and ground water, a study was made on the biodegradation of this surfactant along with other anionic and nonionic surfactants in detergents by water bacteria. Biodegradation was evaluated by the conventional river die-away test employing the methylene-blue method or surface-tension techniques as analytical tools. Under anaerobic conditions, sugar detergents (sucrose esters) seem to be an ultimate solution to water problems created by branched-chain ABS; in aerated water, straight-chain ABS, sulfo tallow methyl esters, and nonionic surfactants are preferable to branched-chain ABS to improve water problems.
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  • 98
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 1-7 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A cost analysis of the continuous fermentation of penicillin G shows that materials cost and product yields are more significant than throughput rate. Number of fermentation stages, productivity, and the implications for research and development are discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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