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  • 1995-1999  (2,299)
  • 1975-1979  (1,002)
  • 1920-1924
  • 1995  (2,299)
  • 1977  (1,002)
  • Cell & Developmental Biology  (2,234)
  • Computational Chemistry and Molecular Modeling  (705)
  • Electron microscopy  (217)
  • Life Sciences
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  • 1995-1999  (2,299)
  • 1975-1979  (1,002)
  • 1920-1924
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  • 1
    Electronic Resource
    Electronic Resource
    The effect of the illumination time when treating port-wine stains (1995)
    Springer
    Lasers in medical science 10 (1995), S. 93-104 
    Details
    ISSN: 1435-604X
    Keywords: Copper vapour laser ; Electron microscopy ; Illumination time ; Numerical modelling ; Optimal treatment ; Port-wine stain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Physics , Technology
    Notes: Abstract This paper reports the electron microscopy results obtained from two patients who were treated with 5 W of yellow (578 nm) light from a copper vapour laser with an illumination time of 3.6 ms and a 0.3 mm spot diameter. The endpoint of treatment was transient blanching. Following treatment, erythema was observed. There was minimal damage to the epidermis and non-vascular tissue such as the nerve fibres. There was severe damage to the endothelial cells of the ectatic vessels. Twenty-four hours after treatment, platelet activation and collagen were present, indicating that these vessels were no longer viable. Theoretical calculations are used to determine the flow of heat within and away from a 50μm diameter vessel. From this, heating of the entire vessel is shown to occur with illumination times of 4 ms, with minimal heating of the non-vascular tissue. Shorter illuminations do not heat the entire vessel, while the use of longer illumination times will cause excessive damage to the surrounding non-vascular tissue. Illumination times close to 4 ms must be regarded as optimal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02150846
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  • 2
    Electronic Resource
    Electronic Resource
    Histological and ultrastructural findings in chloroquine-induced cardiomyopathy (1995)
    Springer
    Journal of molecular medicine 73 (1995), S. 73-77 
    Details
    ISSN: 1432-1440
    Keywords: Chloroquine ; Cardiomyopathy ; Heart ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Light microscopic and ultrastructural findings in patients suffering from chloroquine-induced cardiomyopathy are reviewed. Based on our own observations in an autopsy case, functional and morphological similarities between chloroquine effects and hereditary lysosomal storage diseases are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270580
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  • 3
    Electronic Resource
    Electronic Resource
    Cellular blebbing in superficial colonic epithelial cells occurring with murine graft-versus-host disease (1995)
    Springer
    Virchows Archiv 426 (1995), S. 61-67 
    Details
    ISSN: 1432-2307
    Keywords: Graft-versus-host disease ; Cell blebs ; Light microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Subnuclear blebbing of the superficial colonic epithelium, a rarely described light and electron microscopic change in graft-versus-host disease (GVHD), was studied in a murine model of GVHD. Severity of changes induced by transfer of various donor T cell subsets to irradiated, allogeneic recipients, and association with more severe alterations such as erosions and ulceration were evaluated. By light microscopy the basal region of the superficial enterocytes was greatly expanded by eosinophilic to amphophilic, flocculent, sometimes vacuolated material. By electron microscopy these changes were found to be organelle-poor, cytoplasm-filled protrusions from the basal surface of the epithelium. In this model, helper T cells (CD4+-enriched, CD8+-depleted T cells) transplanted after high dose irradiation were capable of causing the change suggesting cytokine responses may be involved in mediating the cellular injury seen histologically. Close association of blebbing and erosions suggest the blebbing may be the precursor to epithelial erosion or denudation seen in severe intestinal GVHD.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194699
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  • 4
    Electronic Resource
    Electronic Resource
    Culturing of cells from giant cell tumour of bone on natural and synthetic calcified substrata: the effect of leukaemia inhibitory factor and vitamin D3 on the resorbing activity of osteoclast-like cells (1995)
    Springer
    Virchows Archiv 426 (1995), S. 469-477 
    Details
    ISSN: 1432-2307
    Keywords: Giant cell tumour of bone ; In vitro resorption ; Leukaemia inhibitory factor ; Synthetic calcium phosphate ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Osteoclastic cells from giant cell tumour of bone (GCT) of bone provide a rich source for investigation of cellular mechanisms leading to formation of multinucleated cells, the resorption process and involvement of hormones and cytokines in these events. In the present study we investigated the effect of 1,25-dihydroxyvitamin D3 (VD3) and leukaemia inhibitory factor (LIF) on the resorbing potential of osteoclast of GCT origin using quantitative image-analysis of resorption lacunae in an in vitro dentine model. While VD3 unsignificantly increased the number of resorption pits and implicated surface after 7 days of GCT cell culturing, the stimulative effect of LIF was statistically significant. In cultures supplemented with LIF (5000 U/ml) the number of lacunae and resorption surface increased by 38% and 55%, respectively, when compared with control cultures. We suggest that both osteotropic agents increased osteoclastic activity, as the number of multinucleated cells was similar in control and experimental cultures. Seeding of GCT cells on biphasic calcium phosphate substratum revealed the relative inability of osteoclastic cells to resorb this synthetic material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193170
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  • 5
    Electronic Resource
    Electronic Resource
    Von Hippel-Lindau disease presenting as pancreatic neuroendocrine tumour (1995)
    Springer
    Virchows Archiv 426 (1995), S. 523-528 
    Details
    ISSN: 1432-2307
    Keywords: Von Hippel-Lindau disease ; Neuroendocrine tumour ; Electron microscopy ; Flow cytometry ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 21-year-old woman with a family history of von Hippel-Lindau disease presented with a mass in the head of the pancreas. Light microscopic features of the tumour suggested neuroendocrine differentiation and although it displayed positive immunostaining for the antigens expected in a neuroendocrine neoplasm, S-100 staining was also present. This unusual feature prompted further evaluation by routine and post-embedding protein-A gold immunoelectron microscopy, which demonstrated the presence of neuroendocrine granules. Tumour cell DNA content was normal by flow cytometry. Although this patient exhibited no other signs of von Hippel-Lindau disease, the presence of a pancreatic tumour with neuroendocrine differentiation demonstrated that she was affected. Future surveillance and genetic counselling will be influenced by this diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193177
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  • 6
    Electronic Resource
    Electronic Resource
    Some examples of electron microscopy studies of microstructures and phase transitions in solids (1995)
    Springer
    Meccanica 30 (1995), S. 433-438 
    Details
    ISSN: 1572-9648
    Keywords: Electron microscopy ; Microstructures ; Phase transitions ; Solid mechanics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Description / Table of Contents: Sommario Si presentano i risultati di alcuni studi fatti attraverso la microscopia elettronica sulle microstrutture relative a transizioni di fase in una varietà di materiali. I casi comprendono leghe binarie e ternarie, superconduttori TC e materiali C60 e C70; le transizioni esaminate sono diffusionali, displacive o di entrambi i tipi.
    Notes: Abstract In this contribution the results of some electron microscopy studies on microstructures related with phase transitions in a variety of materials will be presented. The materials include binary and ternary alloys, high TC superconductors as well as C60 and C70 fullerenes, while the transitions can be diffusional, displacive or both.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01557075
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  • 7
    Electronic Resource
    Electronic Resource
    Experimental model of chronic tonsillar herniation associated with early stage syringomyelia (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 425-431 
    Details
    ISSN: 1432-0533
    Keywords: Key words Tonsillar herniation ; Syringomyelia ; Chiari malformations ; Histology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes an experimental model of chronic tonsillar herniation and its effects on the spinal cord. In ten rats, a small piece of chemically induced mammary cancer was transplanted to the supraoccipital bone. In all cases, the transplanted cancers grew into the posterior fossa, destroying the supraoccipital bone and compressing the cerebellum extradurally. In six of the ten rats, tonsillar herniation was observed at 8–14 weeks after transplantation. Transdural infiltration of the tumor cells was not apparent in any animal. In those rats with tonsillar herniation (n = 6), the spinal cord from the C5 to the T8 segments showed enlargement of the central canal without exception. Histological examination revealed the following changes: stretching and thinning of the ependymal cells; swelling of the astrocytic processes; and extracellular edema, predominantly in the dorsal gray matter, but also in the ventral inner portion of the dorsal column. In the control group (n = 4) and those rats without tonsillar herniation (n = 4), such histological changes of the spinal cord were not observed. Although the lesions can not be regarded as representing mature syringomyelia, they most likely constitute an earlier evolutionary stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294801
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  • 8
    Electronic Resource
    Electronic Resource
    The morphology of valves and valve-like structures in the canine and feline thoracic duct (1995)
    Springer
    Anatomy and embryology 192 (1995), S. 265-274 
    Details
    ISSN: 1432-0568
    Keywords: Lymphatics ; Valves ; Microanatomy ; Vimentin filaments ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The microanatomy and ultrastructure of the feline and canine thoracic duct and afferent lymphatics were studied by scanning and transmission electron microscopy. We found that the lymphatic vessels were always terminated by ostial valves of two shapes, crescent- and navicular-like, in a ratio of 4∶1. Specific regulatory structures along the free edges of the valves, including marginal thickenings and buttresses, are described. The tissue and cellular organization of the valve endothelium showed distinct peculiarities, particularly in the orientation and shape of the cells and their microrelief. We found that valvular endothelial cells, especially „tip cells”, which are situated in unfavourable lymphodynamic conditions, were characterized by an increased volume density of intermediate (probably vimentin-based) filaments, suggesting an accommodative mechanism involving such filaments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184751
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  • 9
    Electronic Resource
    Electronic Resource
    Experimental model of chronic tonsillar herniation associated with early stage syringomyelia (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 425-431 
    Details
    ISSN: 1432-0533
    Keywords: Tonsillar herniation ; Syringomyelia ; Chiari malformations ; Histology ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This report describes an experimental model of chronic tonsillar herniation and its effects on the spinal cord. In ten rats, a small piece of chemically induced mammary cancer was transplanted to the supraoccipital bone. In all cases, the transplanted cancers grew into the posterior fossa, destroying the supraoccipital bone and compressing the cerebellum extradurally. In six of the ten rats, tonsillar herniation was observed at 8–14 weeks after transplantation. Transdural infiltration of the tumor cells was not apparent in any animal. In those rats with tonsillar herniation (n=6), the spinal cord from the C5 to the T8 segments showed enlargement of the central canal without exception. Histological examination revealed the following changes: stretching and thinning of the ependymal cells; swelling of the astrocytic processes; and extracellular edema, predominantly in the dorsal gray matter, but also in the ventral inner portion of the dorsal column. In the control group (n=4) and those rats without tonsillar herniation (n=4), such histological changes of the spinal cord were not observed. Although the lesions can not be regarded as representing mature syringomyelia, they most likely constitute an earlier evolutionary stage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294801
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  • 10
    Electronic Resource
    Electronic Resource
    Alzheimer paired helical filaments: a comparison with the twisted ribbon model (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 194-197 
    Details
    ISSN: 1432-0533
    Keywords: Key words Alzheimer's disease ; Paired helical ; filaments ; Electron microscopy ; Tau
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate if Alzheimer paired helical filaments (PHF) closely resemble twisted ribbons, as indicated by recent high-resolution ultrastructural studies, we compared physical models of twisted ribbons with electron microscopic images of PHF. Uranyl-acetate-stained, isolated PHF with one or two helical turns were compared with scale models of twisted ribbons with one and two helical turns rotated at different angles. The various rotations of the twisted ribbon model corresponded well with the different orientations of randomly dispersed PHF. The electron-dense regions of individual PHF turns previously thought to represent a cross-over site of paired filaments corresponded to the edge of the twisted ribbon when the ribbon was oriented perpendicular to the filament axis. These data indicate that the overall configuration of PHF is a twisted ribbon but does not exclude possible configuration restrictions due to an ordered arrangement of subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004010050318
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  • 11
    Electronic Resource
    Electronic Resource
    Alzheimer paired helical filaments: a comparison with the twisted ribbon model (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 194-197 
    Details
    ISSN: 1432-0533
    Keywords: Alzheimer's disease ; Paired helical filaments ; Electron microscopy ; Tau
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate if Alzheimer paired helical filaments (PHF) closely resemble twisted ribbons, as indicated by recent high-resolution ultrastructural studies, we compared physical models of twisted ribbons with electron microscopic images of PHF. Uranyl-acetate-stained, isolated PHF with one or two helical turns were compared with scale models of twisted ribbons with one and two helical turns rotated at different angles. The various rotations of the twisted ribbon model corresponded well with the different orientations of randomly dispersed PHF. The electron-dense regions of individual PHF turns previously thought to represent a cross-over site of paired filaments corresponded to the edge of the twisted ribbon when the ribbon was oriented perpendicular to the filament axis. These data indicate that the overall configuration of PHF is a twisted ribbon but does not exclude possible configuration restrictions due to an ordered arrangement of subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294320
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  • 12
    Electronic Resource
    Electronic Resource
    Neomyogenesis in neonatally de-efferented and postnatally denervated rat muscle spindles (1995)
    Springer
    Acta neuropathologica 89 (1995), S. 85-95 
    Details
    ISSN: 1432-0533
    Keywords: Neonatal de-efferentation ; Sensory denervation ; Electron microscopy ; Intrafusal muscle fibre types ; Postnatal myogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of muscle spindles de-efferented by the extirpation of the lumbosacral spinal cord at the age of 2 days and subsequently deprived of their sensory innervation by the section of the sciatic nerve at 3–4 weeks of age was studied in serial sections of 2-month-old rat hindlimb muscles. De-efferentation leaves the primary sensory neurons and their peripheral axons intact and capable of inducing the muscle spindle morphogenesis during the critical period of their development. In de-efferented and subsequently denervated muscle spindles, new supernumerary intrafusal muscle profiles (SIPs) appeared in the muscle spindle A region. They were formed in intimate spatial relation with the original intrafusal muscle fibres (IMFs) predominantly from activated satellite cells derived from both nuclear bag (larger diameter) and nuclear chain fibres. SIPs, however, lacked the typical nuclear accumulations, as well as other ultrastructural distinctions present in control IMFs. The majority of differentiated SIPs separated from original IMFs, whereas the less differentiated SIPs were usually closely apposed to the surface of the parent IMFs and both were covered by the common basal lamina. In some spindles, the original IMFs and/or new SIPs at different stages of their differentiation were found together and they formed clusters of variable shape and composition. In the majority of clusters, all profiles seemed to be isolated along their entire length, although in few clusters, occasional cytoplasmic connections of variable length between intrafusal profiles were found. This result is important for the interpretation of the forthcoming study of expression of muscle spindle-specific myosin heavy chain isoforms in denervated SIPs in rat muscle spindles gradually deprived of their motor and sensory innervation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294263
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  • 13
    Electronic Resource
    Electronic Resource
    Paired helical filaments and straight tubules in astrocytes: an electron microscopic study in dementia of the Alzheimer type (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 31-36 
    Details
    ISSN: 1432-0533
    Keywords: Astrocytes ; Alzheimer's disease ; Electron microscopy ; Paired helical filaments ; Straight tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ultrastructural investigation of the hippocampal CA1 and CA4 of nine sutopsy-proven cases of dementia of the Alzheimer type (duration of disease: 3–16 years; age: 76–92 years) revealed paired helical filaments (PHFs) and straight tubules (STs) in astrocytes of three advanced cases of long duration (〉13years). The PHFs and STs were indistinguishable from those seen in neurons. The abnormal glial fibrils were confined to the astrocytic processes that were associated with small vessels or, more frequently, with ghost tangles. In both locations the astrocytic PHFs and STs were located in the cytoplasm without limiting membranes, and were thicker than the straight filaments that composed ghost tangles. These findings, combined with the presence of regular constrictions of astrocytic PHFs, suggest that abnormal astrocytic fibers are produced by the glial cells, not engulfed by them. In addition, the presence of these abnormal glial filaments in only advanced, long-duration cases of dementia of the Alzheimer type suggests that disease duration has a significant effect upon the formation of these astrocytic profiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294456
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  • 14
    Electronic Resource
    Electronic Resource
    Paired helical filaments and straight tubules in astrocytes: an electron microscopic study in dementia of the Alzheimer type (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 31-36 
    Details
    ISSN: 1432-0533
    Keywords: Key words Astrocytes ; Alzheimer's disease ; Electron microscopy ; Paired helical filaments ; Straight tubules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Ultrastructural investigation of the hippocampal CA1 and CA4 of nine autopsy-proven cases of dementia of the Alzheimer type (duration of disease: 3–16 years; age: 76–92 years) revealed paired helical filaments (PHFs) and straight tubules (STs) in astrocytes of three advanced cases of long duration (〉13 years). The PHFs and STs were indistinguishable from those seen in neurons. The abnormal glial fibrils were confined to the astrocytic processes that were associated with small vessels or, more frequently, with ghost tangles. In both locations the astrocytic PHFs and STs were located in the cytoplasm without limiting membranes, and were thicker than the straight filaments that composed ghost tangles. These findings, combined with the presence of regular constrictions of astrocytic PHFs, suggest that abnormal astrocytic fibers are produced by the glial cells, not engulfed by them. In addition, the presence of these abnormal glial filaments in only advanced, long-duration cases of dementia of the Alzheimer type suggests that disease duration has a significant effect upon the formation of these astrocytic profiles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294456
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  • 15
    Electronic Resource
    Electronic Resource
    Activity of acetylcholinesterase and unspecific cholinesterase during differentiation of somites in mouse embryos (1995)
    Springer
    Anatomy and embryology 192 (1995), S. 257-264 
    Details
    ISSN: 1432-0568
    Keywords: Cholinesterases ; Electron microscopy ; Development ; Mouse embryo ; Somites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract During the development of somites in mouse embryos, widespread activity of unspecific cholinesterase (BuChE) was demonstrated after prolonged incubation. Independent of their position, all somite cells and their derivatives (dermatome, myotome and sclerotome) exhibited enzyme activity in the perinuclear space and in the endoplasmic reticulum. The plasmalemma did not show any enzyme activity. Differentiation of the sclerotome into vertebrae was accompanied by a reduction of BuChE. However, a low enzyme reaction was still present in the first typical differentiated chondroblasts. Notochordal cells were detectable by their high BuChE content. This was also found in cells already showed severe degeneration. In addition to BuChE, acetylcholinesterase (AChE) was first visible on day 9 of embryonic development in newly formed myotubes of the myotomes. Some hypotheses on the functional significance of embryonic BuChE are discussed in the light of these results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00184750
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  • 16
    Electronic Resource
    Electronic Resource
    The anatomy of bone sialoprotein immunoreactive sites in bone as revealed by combined ultrastructural histochemistry and immunohistochemistry (1995)
    Springer
    Calcified tissue international 57 (1995), S. 277-284 
    Details
    ISSN: 1432-0827
    Keywords: Bone sialoprotein ; osteoblast ; Bone matrix ; Electron microscopy ; Immunolocalization ; noncollagenous protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Bone sialoprotein was immunolocalized at the EM level in thin Lowicryl K4M sections of rat bone. Because of the unconventional EM morphology of the bone matrix seen in thin demineralized acrylate sections, the pattern of immunolabeling was compared with detailed structural images of demineralized bone obtained using an en bloc treatment of tissue samples with the cationic electron ‘dye’, Malachite Green (MG), which provides stabilization and retention of anionic material throughout specimen processing. A system of structures corresponding to the sites of bone sialoprotein (BSP) immunoreactivity, as seen in Lowicryl K4M thin sections, could be readily identified in the MG-treated, expoxy thin sections. This system includes the cement lines, and aggregates of similar material within mineralized bone and mineralizing osteoid. The virtual identity of BSP distribution with the arrangement of the MG-visualized material indicates that a BSP-enriched, noncollagenous phase can be demonstrated using different, unrelated tissue preparation and imaging protocols for EM. Besides improving our understanding of the distribution of bone sialoprotein in bone, these data assign a previously unrecognized structural dimension to noncollagenous material in the bone matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298883
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  • 17
    Electronic Resource
    Electronic Resource
    An ultrastructural evaluation of the effects of cysteine-proteinase inhibitors on osteoclastic resorptive functions (1995)
    Springer
    Calcified tissue international 56 (1995), S. 566-570 
    Details
    ISSN: 1432-0827
    Keywords: Cathepsin inhibitors ; Osteoclasts ; Resorption ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract This study was designed to evaluate the effects of specific and potent cathepsin inhibitors on osteoclastic resorptive functions in vitro by means of a novel ultrastructural assay system. Mouse bone marrow cell-derived osteoclasts were suspended on dentine slices and cultured for 48 hours in the presence of either E-64 (a generalized cysteine proteinase inhibitor) or Z-Phe-Phe-CHN2 (a selective cathepsin L inhibitor). After the removal of cultured osteoclasts, co-cultured dentine slices were examined using electron microscopy: backscattered (BSEM), scanning (SEM), and atomic force (AFM). In morphometric analyses of BSEM images, there were no significant differences in the areas of demineralized dentine surfaces between control and inhibitor-treated groups, suggesting that cathepsin inhibitors had no effect on dentine demineralization by cultured osteoclasts. However, in SEM and AFM observations, both inhibitors remarkably reduced to the same extent, the formation of deep resorption lacunae on dentine slices that had resulted from degradation of matrix collagen. In addition, Z-Phe-Phe-CHN2 treatment produced deeper, ring-like grooves with little collagen exposure in shallow resorption lacunae. These results strongly suggest that (1) cathepsins released by osteoclasts are involved in the formation of deep resorption lacunae, and (2) cathepsin L plays a key role in bone resorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298591
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  • 18
    Electronic Resource
    Electronic Resource
    Ultrastructure and mucociliary transport of bronchial respiratory epithelium in intubated patients (1995)
    Springer
    Intensive care medicine 21 (1995), S. 482-489 
    Details
    ISSN: 1432-1238
    Keywords: Mucociliary clearance ; Ultrastructural ciliary alterations ; Cilia ; Electron microscopy ; Intubation ; Mechanical ventilation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objective The objective of this study was to investigate whether reduced bronchial mucus transport velocity (BTV) is associated with a loss of cilia or ultrastructural abnormalities of cilia in intubated patients. Design The patients were studied prospectively in a convenience sample trial. Setting The study took place in a university hospital. Patients and participants 29 orally intubated patients in a surgical ICU. Interventions BTV was measured with radiolabeled microspheres in the right and left primary bronchus. Following these measurements, biopsy samples were taken from the bronchi for scanning (SEM) and transmission (TEM) electron-microscopic investigations. Measurements and results SEM: Patients with normal or slight impaired BTV (group 1,n=14: BTV: 8.5 mm/min (3.8–11.5); median with range) showed more cilia on the luminal surface than patients with markedly depressed BTV (p〈0.05) (group 2,n=15: BTV: 0(0–2.1)). The difference was statistically significant. The BTV values correlated moderately with the number of cilia on the luminal surface (r=0.46;p=0.02). TEM: In group 1, 6.5% (3.9–14.9) of cilia were abnormal (median with range) vs 9.3% (4.9–13.7) in group 2; these differences were not statistically significant. Neither was there any significant correlation between BTV and the frequency of abnormal cilia. Conclusions Impaired mucociliary transport in intubated patients is associated with a loss of cilia rather than ultrastructural abnormalities of cilia, which are less relevant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01706201
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  • 19
    Electronic Resource
    Electronic Resource
    Angiokeratoma corporis diffusum ohne assoziierte Stoffwechselstörung (1995)
    Springer
    Der Hautarzt 46 (1995), S. 785-788 
    Details
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Angiokeratoma corporis diffusum ; Lysosomale Stoffwechselstörung ; Elektronenmikroskopie ; Kapillarmikroskopie ; Key words Angiokeratoma corporis diffusum ; Lysosomal disorder ; Electron microscopy ; Capillary microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary In a 22-year-old woman with mental impairment and some signs of dysmorphism, numerous angiokeratomas developed, starting when she was 3 years old and resulting in the clinical picture of angiokeratoma corporis diffusum. Extensive laboratory analyses did not disclose any associated metabolic disorder, and electron microscopy failed to demonstrate lysosomal inclusions. Therefore, this patient can be classified as one of the rare cases of angiokeratoma corporis diffusum without associated metabolic disease (idiopathic angiokeratoma corporis diffusum).
    Notes: Zusammenfassung Bei einer 22jährigen Patientin mit ausgedehnten, ab dem 3. Lebensjahr auftretenden Angiokeratomen im Sinne eines Angiokeratoma corporis diffusum, welche zusätzlich eine Intelligenzminderung und Dysmorphiezeichen aufwies, konnte trotz intensiver Suche laborchemisch keine assoziierte Stoffwechselstörung gefunden werden. Der fehlende Nachweis einer lysosomalen Speicherung in der Elektronenmikroskopie stützte diesen Befund. Somit liegt bei dieser Patientin die seltene Situation eines Angiokeratoma corporis diffusum ohne assoziierte Stoffwechselkrankheit (idiopathisches Angiokeratoma corporis diffusum) vor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001050050339
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  • 20
    Electronic Resource
    Electronic Resource
    Nerval-Neuroendokrine Komplexe in Magenschleimhaut bei Zollinger-Ellison-Syndrom (1995)
    Springer
    Der Pathologe 16 (1995), S. 404-407 
    Details
    ISSN: 1432-1963
    Keywords: Schlüsselwörter Nerval-neuroendokriner Komplex ; Zollinger-Ellison-Syndrom ; Magenschleimhaut ; Elektronenmikroskopie ; Key words Nerve fibre-neuroendocrine cell complex ; Stomach ; Zollinger-Ellison-Syndrom ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Nerve fibre-neuroendocrine cell complexes (NF-NEC-C's) are neuroendocrine cells located in the lamina propria of the gastrointestinal tract directly connected with nerve fibres of Meissner's plexus. We report on a patient with sporadic Zollinger-Ellison syndrome (ZES) with electron microscopically demonstrated multiple NF-NEC-C's in non-antral gastric mucosa. It is suspected that in ZES the hypergastrinaemia may represent a trophic stimulus for the proliferation of NF-NEC-C's in the gastric mucosa.
    Notes: Zusammenfassung Unter nerval-neuroendokrinen Komplexen (N-NE-K) versteht man in der Lamina propria mucosae des Magen-Darm-Traktes gelegene Nester neuroendokriner Zellen, die in direktem Kontakt zu Nervenfasern des Meissner-Plexus stehen. Es wird über einen Patienten mit sporadischem Zollinger-Ellison-Syndrom (ZES) berichtet, bei dem sich elektronenmikroskopisch in nonantraler Magenschleimhaut multiple N-NE-K fanden. Möglicherweise stellt die Hypergastrinämie bei ZES einen trophischen Stimulus für die Proliferation von N-NE-K in der Magenschleimhaut dar.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002920050121
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  • 21
    Electronic Resource
    Electronic Resource
    Quaternary structure of the hydroxylamine oxidoreductase from Nitrosomonas europaea (1995)
    Springer
    Archives of microbiology 163 (1995), S. 300-306 
    Details
    ISSN: 1432-072X
    Keywords: Nitrosomonas europea ; Hydroxylamine oxidoreductase (HAO) ; Electron microscopy ; Electron spectroscopic imaging ; Quaternary structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hydroxylamine oxidoreductase from Nitrosomonas europaea was prepared to apparent electrophoretic homogeneity. Electron microscopy of negatively stained preparations of the sample revealed an overall diameter of about 8.8 nm of the enzyme particle. The native structure was determined as a tetrahedron-like assembly of identical subunits exhibiting four protein masses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393384
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  • 22
    Electronic Resource
    Electronic Resource
    Quaternary structure of the hydroxylamine oxidoreductase from Nitrosomonas europaea (1995)
    Springer
    Archives of microbiology 163 (1995), S. 300-306 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsNitrosomonas europea ; Hydroxylamine ; oxidoreductase (HAO) ; Electron microscopy ; Electron ; spectroscopic imaging ; Quaternary structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hydroxylamine oxidoreductase from Nitrosomonas europaea was prepared to apparent electrophoretic homogeneity. Electron microscopy of negatively stained preparations of the sample revealed an overall diameter of about 8.8 nm of the enzyme particle. The native structure was determined as a tetrahedron-like assembly of identical subunits exhibiting four protein masses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00393384
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  • 23
    Electronic Resource
    Electronic Resource
    Exazerbation eines Morbus Hailey-Hailey durch Infektion mit Herpes-simplex-Virus Nachweis mittels Polymerasekettenreaktion (1995)
    Springer
    Der Hautarzt 46 (1995), S. 494-497 
    Details
    ISSN: 1432-1173
    Keywords: Schlüsselwörter Morbus Hailey-Hailey ; Pemphigus chronicus benignus familiaris ; Polymerasekettenreaktion ; Herpes-simplex-Virus ; Elektronenmikroskopie ; Histologie ; Key words Hailey-Hailey disease ; Benign familial pemphigus ; Polymerase chain reaction ; Herpes simplex virus ; Electron microscopy ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 49-year-old man suffered from Hailey-Hailey disease for several years. The patient presented with an acute exacerbation of the disease, which did not respond to oral treatment with high doses of glucocorticosteroids. A skin biopsy was taken and the histological examination indicated a viral infection. Herpes simplex virus was confirmed by electron microscopy (negative staining) and polymerase chain reaction (PCR). The PCR presents a sensitive and effective molecular-biological method for the diagnosis of viral infections.
    Notes: Zusammenfassung Es wird über einen 49jährigen Patienten berichtet, bei dem seit Jahren ein Morbus Hailey-Hailey bekannt ist. Bei einem Rezidiv der Erkrankung kam es trotz hoher Glukokortikosteroidgaben zu keiner Remission. Erst die erneute diagnostische Abklärung des Rezidivs durch eine Hautprobe ergab den Verdacht auf eine Herpessimplex-Virus-Infektion, die über elektronenmikroskopische Negativkontrastierung und über Polymerasekettenreaktion (PCR) bestätigt werden konnte. Anhand dieser Kasuistik wird auf ein neueres molekularbiologisches Verfahren hingewiesen, das eine sensitive und schnelle Methode zum Virusnachweis darstellt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001050050289
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  • 24
    Electronic Resource
    Electronic Resource
    Progressive liver failure in a patient with adult Niemann-Pick disease associated with generalized AL amyloidosis (1995)
    Springer
    Virchows Archiv 426 (1995), S. 635-639 
    Details
    ISSN: 1432-2307
    Keywords: Adult Niemann-Pick disease ; Generalized AL-amyloidosis ; Progressive liver failure ; Fibroblast culture ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We report a case in which an adult form of Niemann-Pick disease (type B of NPD) was associated with a rapidly progressive generalized AL amyloidosis of kappa type. Both diagnosis were made by biopsy, the NPD by bone marrow biopsy and fibroblast culture, the amyloidosis by liver biopsy. Malignant non-Hodgkin lymphoma was not found. The patient, a 67-year-old woman, died from hepatic coma subsequent to a progressive liver failure. We discuss possible relations between the lysosomal storage disease and the development and rapid progression of amyloidosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00192120
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  • 25
    Electronic Resource
    Electronic Resource
    Horseradish peroxidase in axons of the dorsal funiculi of the cat: distribution after an injection of the enzyme into the dorsal column nuclei (1995)
    Springer
    Cell & tissue research 283 (1995), S. 67-73 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Node of Ranvier ; Axon ; Horseradish peroxidase (HRP) ; Retrograde axonal transport ; Electron microscopy ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Horseradish peroxidase (HRP) was injected into the left dorsal column nuclei of adult cats. Large dorsal funiculi axons of the C3, C5, C8 and L7 segments were searched for HRP-activity after 12, 24, 36 and 48 h using light and electron microscopy. Accumulations of intra-axonal HRP-positive bodies occurred at nodes of Ranvier in the C3–C8 segments at 12, 24 and 36 h and in the L7 segments at 24, 36, and 48 h. The accumulations of HRP in three spatio-temporally different consecutive patterns, noted earlier at nodes of Ranvier in the peripheral nervous system (PNS) portion of feline alpha motor axons for more than 70 h after an intramuscular injection of the enzyme, were not observed in the present material. We suggest that the differences in the modes in which large PNS and CNS axons interact with retrogradely transported HRP are due to differences in the organization of the respective nodal regions. We also emphasize that endocytosis via axon terminals in the CNS normally represents uptake of material from an extracellular space which is controlled and protected by the blood-brain barrier. This is in contrast to endocytosis via axon terminals in a muscle, which represents uptake of material from an extracellular space openly exposed to influx of different substances from the blood stream.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050513
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  • 26
    Electronic Resource
    Electronic Resource
    FMRFamide-like and allatostatin-like immunoreactivity in the lateral heart nerve of Periplaneta americana: colocalization at the electron-microscopic level (1995)
    Springer
    Cell & tissue research 282 (1995), S. 69-80 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Lateral heart nerve ; FMRFamide ; Allatostatin ; Coexistence ; Mirror-section ; Axosomatic synapses ; Electron microscopy ; Periplaneta americana (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Both allatostatin immunoreactivity (AS-IR) and FMRFamide immunoreactivity (FMRFa-IR) have been demonstrated light-microscopically in the lateral heart nerve of Periplaneta americana. The identical labeling of some fibers suggests the coexistence of the two antigens. Electron-microscopically, six granule types in the peripheral part of the lateral heart nerve can be distinguished according to their size and density (types 1–6). These granule types can be subdivided immunocytochemically by means of a new mirror-section technique. Granules of types 4 and 5 always exclusively show FMRFa-IR. In the populations of fibers containing granules of types 1 and 6, axon profiles can be found that contain granules colocalizing FMRFa-IR and AS-IR. Other axon profiles of these populations only contain immunonegative granules of the same ultrastructure. Granules of type 2 can be differentiated immunocytochemically in three forms in the same section: In some fibers, they are nonreactive; in other fibers of the same section, they show FMRFa – IR, whereas in a third fiber type, granules show AS – IR. Finally, granules of type 3 can be observed with FMRFa-IR. In other fibers, they occur with the same ultrastructure but exhibit no immunoreactivity. Two soma types occur in the lateral heart nerve. Soma type I is characterized by the production of electron-dense granules that show FMRFa-IR. Type II is in close contact with various fibers, forming different types of axosomatic synapses, hitherto unknown in Insecta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050460
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  • 27
    Electronic Resource
    Electronic Resource
    Electron-microscopic localisation of thiol and disulphide groups by direct monomaleimido-nanogold labelling in the spermatozoa of a marsupial, the tammar wallaby (Macropus eugenii) (1995)
    Springer
    Cell & tissue research 282 (1995), S. 291-296 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Spermatozoa ; Acrosome ; Thiols ; Disulphides ; Electron microscopy ; Monomaleimido nanogold ; Macropus eugenii (Marsupialia)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. This study utilised a commercially available monomaleimido-nanogold reagent to directly label cellular thiol groups (SH) of marsupial (tammar wallaby) spermatozoa before and after reduction of disulphides (S-S) with mercaptoethylamine hydrochloride (MEA). The sperm surface, mitochondrial membranes, axoneme and tail fibres were all labelled with gold particles before MEA treatment and the label intensity was increased after S-S reduction. The acrosomal membranes and matrix of spermatozoa contained no detectable SH prior to MEA treatment. However, after moderate MEA treatment (1 mg/ml) gold label was associated with the acrosomal membrane and invaginated acrosomal membrane within the acrosomal matrix. After exposure to 5 and 10 mg/ml MEA, gold particles heavily labelled the acrosomal matrix. Thus, the acrosomal membranes and matrix of tammar wallaby spermatozoa both contain S-S cross-linked structures, and this may contribute to the unusual stability of the marsupial acrosome. Under all treatment conditions the nucleus remained unlabelled. This is consistent with early studies which indicated that cysteine was absent from the nuclear protamines. The study also demonstrated that monomaleimido-nanogold can be used to resolve SH- and S-S-rich cellular structures directly, in addition to its use to label antibodies and Fab fragments for immunochemical localisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050480
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  • 28
    Electronic Resource
    Electronic Resource
    Dynamics of secretory granules in somatotrophs of rats after stimulation with growth hormone-releasing factor: a stereological analysis (1995)
    Springer
    Cell & tissue research 282 (1995), S. 493-501 
    Details
    ISSN: 1432-0878
    Keywords: Somatotrophs ; Growth hormone ; Growth hormone-releasing factor ; Secretory granule ; Electron microscopy ; Morphometry ; Rat (Fischer 344)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The anterior pituitary tissue of male rats injected with growth hormone-releasing factor (GRF) was either processed for stereology at the light-and electron-microscopic levels, or homogenized for growth hormone (GH) assay 2–60 min after GRF injection. Secretory granules of somatotrophs became smaller but increased in numerical density 2 min after GRF injection. Their volume density began to increase at 5 min. The frequency of exocytosis of the granules was most prominent as early as 2 min after GRF injection and reduced thereafter. GH levels in the tissue were lowest at 2–5 min, and returned to the control value by 60 min. Serum GH levels were highest at 15 min; even at 60 min, this value was higher than in the controls. These findings suggest that secretory granules in somatotrophs are stimulated to divide by GRF, resulting in a decrease in size and an increase in number. The discrepancy between the earlier formation of new secretory granules and the later restoration of intracellular GH levels implies that GRF first stimulates the synthesis of constituents of granules other than GH, and only later the synthesis of GH, and that newly formed small secretory granules contain less GH. From the clearance rate of serum GH and the frequency of granule exocytosis, it can be estimated that about a half million granules are released to maintain 1 ng/ml of serum GH in rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318881
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  • 29
    Electronic Resource
    Electronic Resource
    Decreased adhesion of oxidized LDL-stimulated platelets caused by cytochalasin D (1995)
    Springer
    Cell & tissue research 280 (1995), S. 183-188 
    Details
    ISSN: 1432-0878
    Keywords: Blood platelets ; Oxidized LDL ; Cytochalasin D ; Reflection contrast microscopy ; Image analysis ; Electron microscopy ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The adhesion of human blood platelets is studied with an in vitro model using reflection contrast microscopy and an image analysis system. The adhesive feature is promoted by oxidatively modified low density lipoprotein, which also induces functional morphological changes of platelets. However, when washed platelets are pretreated with 0.05 mM cytochalasin D, oxidized low density lipoprotein (100 μg/ml) causes a slower increase of the adhesion area (11.6 μm2/min) compared to untreated platelets (15.7 μm2/min) or platelets treated by oxidized low density lipoprotein alone (20.5 μm2/min, P〈0.01). These results are supported by light transmission analysis and by transmission electron microscopy. Our experiments suggest that cytochalasin D inhibits the change of platelets in shape induced by oxidized low density lipoprotein, hinders the adhesion, but does not prevent the adhesion entirely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304523
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  • 30
    Electronic Resource
    Electronic Resource
    Do vasoactive intestinal peptide (VIP)-and nitric oxide synthase-immunoreactive terminals synapse exclusively with VIP cell bodies in the submucous plexus of the guinea-pig ileum? (1995)
    Springer
    Cell & tissue research 281 (1995), S. 485-491 
    Details
    ISSN: 1432-0878
    Keywords: Nitric oxide synthase ; Vasoactive intestinal peptide ; Immunohistochemistry ; Electron microscopy ; Submucous plexus ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In the submucous plexus of the guinea-pig ileum, previous light-microscopic studies have revealed that vasoactive intestinal peptide (VIP)-immunoreactive and nitric oxide synthase (NOS)-immunoreactive terminals are found predominantly in association with VIP-immunoreactive nerve cell bodies. In this study, double-label immunohistochemistry at the light-microscopic level demonstrated co-localization of NOS-immunoreactivity and VIP-immunoreactivity in axon terminals in submucous ganglia. About 90% of nerve fibres with NOS-immunoreactivity or VIP-immunoreactivity were immunoreactive for both antigens; only about 10% of labelled varicosities contained only NOS-immunoreactivity or VIP-immunoreactivity. The VIP/NOS varicosities were more often seen in the central parts of the ganglia, close to the VIP-immunoreactive cell bodies. Ultrastructural immunocytochemistry with antibodies to VIP was used to determine if NOS/VIP terminals synapse exclusively with VIP-immunoreactive nerve cell bodies. We examined the targets of VIP-immunoreactive boutons in two submucous ganglia from different animals. Serial ultrathin sections were taken through the ganglia after they had been processed for VIP immunocytochemistry. For each cell body, the number of VIP inputs (synapses and close contacts) was determined. The number of VIP-immunoreactive synapses received by the cell bodies of submucous neurons varied from 0–4 and the number of VIP-immunoreactive close contacts varied from 3–10. There was no significant difference between VIP-immunoreactive nerve cell bodies and non-VIP nerve cell bodies in the number of VIP-immunoreactive synapses and close contacts they received. Thus, the implication from light microscopy that NOS/VIP terminals end predominantly on VIP nerve cells was not vindicated by electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00417865
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  • 31
    Electronic Resource
    Electronic Resource
    Localization of CD44, the hyaluronate receptor; on the plasma membrane of osteocytes and osteoclasts in rat tibiae (1995)
    Springer
    Cell & tissue research 280 (1995), S. 225-233 
    Details
    ISSN: 1432-0878
    Keywords: CD44, adhesion molecule ; Bone ; Osteoclasts ; Osteocytes ; Immunohistochemistry ; Confocal laser scanning microscopy ; Electron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract CD44 is a multifunctional adhesion molecule that binds to hyaluronic acid, type I collagen, and fibronectin. We have studied the immunohistochemical localization of CD44 in bone cells by confocal laser scanning microscopy and transmission electron microscopy in order to clarify its role in the cell-cell and/or cell-matrix interaction of bone cells. In round osteoblasts attached to bone surfaces, immunoreactivity is restricted to their cytoplasmic processes. On the other hand, osteocytes in bone matrices show intense immunoreactivity on their plasma membrane. Intense immunoreactivity for CD44 can be detected on the basolateral plasma membranes of osteoclasts. There is considerably less reactivity observed in the area of the plasma membrane that is in direct contact with bone. The pre-embedding electron-microscopical method has revealed that CD44 is mainly localized on the basolateral plasma membrane of osteoclasts. However, the ruffled border and clear zone show little immunoreactivity. A CD44-positive reaction can be detected on both plasma membranes in the contact region between osteoclasts and osteocytes. These findings suggest that: 1) cells of the osteoblast lineage express CD44 in accordance with their morphological changes from osteoblasts into osteocytes; 2) osteoclasts express CD44 on their basolateral plasma membrane; 3) CD44 in osteoclasts and osteocytes may play an important role in cell-cell and/or cell-matrix attachment via extracellular matrices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307793
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  • 32
    Electronic Resource
    Electronic Resource
    Ultrastructural investigation of nitric oxide synthase-immunoreactive nerves associated with coronary blood vessels of rat and guinea-pig (1995)
    Springer
    Cell & tissue research 280 (1995), S. 575-582 
    Details
    ISSN: 1432-0878
    Keywords: Nitric oxide synthase ; Coronary vasculature ; Electron microscopy ; Immunocytochemistry Rat (sprague Dawley) ; Guinea-pig (Dunkin Hartley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ultrastructural investigation of nitrix oxide synthase-immunoreactive nerves closely associated with blood vessels in rat and guinea-pig hearts revealed many labelled nerve fibres in the walls of the main branches of the coronary arteries, and in arterioles, capillaries and post-capillary venules. The number of nitric oxide synthase-containing nerve fibres associated with different vessels, even those of the same calibre, varied. Terminal regions of nitric oxide synthase-immunoreactive fibres were observed in the endocardium and myocardium. Nitric oxide synthase-labelled fibres displayed electrondense immunoproduct in both varicose and intervaricose regions. Immunoreactive axonal varicosities contained both small and large synaptic vesicles. The characteristics of the nitric oxide synthase-immunoreactive nerve fibres observed in the heart and the possibility that these fibres represent the processes of intracardiac neurones and/or sensory neurones of extrinsic origin are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318361
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  • 33
    Electronic Resource
    Electronic Resource
    Ultrastructural study of periodic lamellar granules in human neutrophils (1995)
    Springer
    Cell & tissue research 281 (1995), S. 69-76 
    Details
    ISSN: 1432-0878
    Keywords: Periodic lamellar granule ; Primary granule ; Chronic myeloproliferative disorder ; Neutrophils ; Electron microscopy ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Granules consisting of periodically arranged membranous lamellae and amorphous electron-opaque material, i.e., periodic lamellar granules, are present in human neutrophils. To date, no extensive ultrastructural studies have been carried out on these granules because of their infrequent presence in neutrophils. The bone marrow of 18 cases of chronic myeloproliferative disorders, including one case of chronic neutrophilic leukemia in which periodic lamellar granules were frequently seen in neutrophils, was investigated by electron microscopy. Periodic lamellar granules were seen in neutrophils in 12 of the 18 cases at varying frequencies. They were preferentially seen in immature neutrophils. The transverse profiles of these granules revealed concentric complete/incomplete rings or periodic parallel straight lines, i.e., various patterns of lamellar arrangement were present. Periodic lamellar granules were positive for myeloperoxidase and lysozyme at the electron-microscopic level. These results suggest that these granules represent a primary neutrophil granule subtype. However, their functional and pathologic significance remains unknown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307959
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  • 34
    Electronic Resource
    Electronic Resource
    Investigation of the structural and functional features of splenic ellipsoids in rainbow trout (Oncorhynchus mykiss) (1995)
    Springer
    Cell & tissue research 279 (1995), S. 469-474 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Ellipsoids ; Spleen ; Electron microscopy ; Fluorescent microspheres ; Oncorhynchus mykiss (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The ultrastructure of ellipsoids in the spleen of rainbow trout (Oncorhynchus mykiss) is described. The endothelium of terminations of arterioles bulged into the lumen, and gaps between the endothelial cells were evident. A continuous basal lamina was not present, and there were extensive interdigitations between the endothelial cells and surrounding reticular cells. The interdigitating processes were rich in microfilaments. Intravenously injected colloidal carbon, approximately 0.03 μm in diameter, was held in the reticular matrix of the ellipsoidal wall and taken up by macrophages that extended cellular processes among the reticular and endothelial cells. The intravenous injection of fluorescent polystyrene microspheres of known diameter showed that microspheres with a diameter of 0.5 or 1.0 μm were localised in the red pulp, whereas microspheres with a diameter of 0.15 μm were retained in ellipsoidal walls. Thus, the terminations of splenic arterioles in rainbow trout were found to be consistent with descriptions of ellipsoids in other vertebrates in that they possessed a specialised cuboidal endothelium, lacked a continuous basal lamina, were surrounded by a sheath of macrophages and reticular cells, and had a role in the selective filtration and retention of blood-borne particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050307
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  • 35
    Electronic Resource
    Electronic Resource
    FMRFamide-like and allatostatin-like immunoreativity in the lateral heart nerve of Periplaneta americana: Colocalization at the electron-microscopic level (1995)
    Springer
    Cell & tissue research 282 (1995), S. 69-80 
    Details
    ISSN: 1432-0878
    Keywords: Lateral heart nerve ; FMRFamide ; Allatostatin ; Coexistence ; Mirror-section ; Axosomatic synapses ; Electron microscopy ; Periplaneta americana (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Both allatostatin immunoreactivity (AS-IR) and FMRFamide immunoreactivity (FMRFa-IR) have been demonstrated light-microscopically in the lateral heart nerve of Periplaneta americana. The identifical labeling of some fibers suggests the coexistence of the two antigens. Electron-microscopically, six granule types in the peripheral part of the lateral heart nerve can be distinguished according to their size and density (types 1–6). These granule types can be subdivided immunocytochemically by means of a new mirror-section technique. Granules of types 4 and 5 always exclusively show FMRFa-IR. In the populations of fibers containing granules of types 1 and 6, axon profiles can be found that contain granules colocalizing FMRFa-IR and AS-IR. Other axon profiles of these populations only contain immunonegative granules of the same ultrastructure. Granules of type 2 can be differentiated immunocytochemically in three forms in the same section: In some fibers, they are nonreactive; in other fibers of the same section, they show FMRFa-IR, whereas in a third fiber type, granules show AS-IR. Finally, granules of type 3 can be observed with FMRFA-IR. In other fibers, they occur with the same ultrastructure but exhibit no immunoreactivity. Two soma types occur in the lateral heart nerve. Soma type I is characterized by the production of electron-dense granules that show FMRFa-IR. Type II is in close contact with various fibers, forming different types of axosomatic synapses, hitherto unknown in Insecta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319134
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  • 36
    Electronic Resource
    Electronic Resource
    Catecholaminergic innervation of the suprachiasmatic nucleus in the adult rat: ultrastructural relationships with neurons containing vasoactive intestinal peptide or vasopressin (1995)
    Springer
    Cell & tissue research 280 (1995), S. 87-96 
    Details
    ISSN: 1432-0878
    Keywords: Suprachiasmatic nucleus ; Catecholamines ; Dopamine ; Electron microscopy ; Vasoactive intestinal peptide ; Vasopressin ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Catecholaminergic fibers in the suprachiasmatic nucleus of adult rats were investigated by use of light- and electron-microscopic immunocytochemistry. The suprachiasmatic nucleus receives a modest density of tyrosine hydroxylase-containing axons, homogeneously distributed in the nucleus and forming varicosities throughout its entire rostro-caudal extension. Immunolabeling with antibodies against dopamine showed that this catecholamine input comprises a dopaminergic component. Many tyrosine hydroxylase-positive cells were localized at the immediate periphery of the suprachiasmatic nucleus. With electron-microscopic examination, dendrites of these neurons were found within the limits of the nucleus as well as at a border zone between the suprachiasmatic nucleus proper and the optic tract where they received unlabeled synapses, providing a morphological support for a possible role of dopaminergic neurons in the integration and/or transfer of light-related signals. More than 91% of catecholaminergic axonal varicosities were found to establish morphologically defined synapses with dendrites. To investigate whether these synapses might be shared with neurons of one or both of the two main peptidergic populations of the nucleus, namely vasoactive intestinal peptide- and vasopressin-containing neurons, we carried out doublelabeling experments combining immunoperoxidase and immunogold-silver labeling. Results showed only a few cases of direct association of the catecholaminergic terminals with these peptidergic categories. In both types of dually stained sections, catecholaminergic synapses were preferentially made with unlabeled dendrites. The homogeneous distribution of tyrosine hydroxylase-immunoreactive fibers in the suprachiasmatic nucleus could therefore reflect a lack of significant catecholaminergic innervation of both vasoactive intestinal peptide- and vasopressin-synthesizing neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304514
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  • 37
    Electronic Resource
    Electronic Resource
    Origin and destination of globules and irregular masses in the gonadotropin cells from the pituitary of the African catfish, Clarias gariepinus: a morphological study (1995)
    Springer
    Cell & tissue research 280 (1995), S. 113-122 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Gonadotrops ; Crinophagy ; Electron microscopy ; Enzyme cytochemistry ; Immunocytochemistry ; Autoradiography ; Catfish, Clarias gariepinus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demonstrated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against α and β gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the β subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304516
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  • 38
    Electronic Resource
    Electronic Resource
    Origin and destination of globules and irregular masses in the gonadotropin cells from the pituitary of the African catfish, Clarias gariepinus:a morphological study (1995)
    Springer
    Cell & tissue research 280 (1995), S. 113-122 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Pituitary ; Gonadotrops ; Crinophagy ; Electron microscopy ; Enzyme cytochemistry ; Immunocytochemistry ; Autoradiography ; Catfish ; Clarias gariepinus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demons trated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against α and β gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the β subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses.
    Type of Medium: Electronic Resource
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  • 39
    Electronic Resource
    Electronic Resource
    Decreased adhesion of oxidized LDL-stimulated platelets caused by cytochalasin D (1995)
    Springer
    Cell & tissue research 280 (1995), S. 183-188 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Blood platelets ; Oxidized LDL ; Cytochalasin D ; Reflection contrast microscopy ; Image analysis ; Electron microscopy ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The adhesion of human blood platelets is studied with an in vitro model using reflection contrast microscopy and an image analysis system. The adhesive feature is promoted by oxidatively modified low density lipoprotein, which also induces functional morphological changes of platelets. However, when washed platelets are pretreated with 0.05 mM cytochalasin D, oxidized low density lipoprotein (100 μg/ml) causes a slower increase of the adhesion area (11.6 μm2/min) compared to untreated platelets (15.7 μm2/min) or platelets treated by oxidized low density lipoprotein alone (20.5 μm2/min, P〈0.01). These results are supported by light transmission analysis and by transmission electron microscopy. Our experiments suggest that cytochalasin D inhibits the change of platelets in shape induced by oxidized low density lipoprotein, hinders the adhesion, but does not prevent the adhesion entirely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304523
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  • 40
    Electronic Resource
    Electronic Resource
    Catecholaminergic innervation of the suprachiasmatic nucleus in the adult rat: ultrastructural relationships with neurons containing vasoactive intestinal peptide or vasopressin (1995)
    Springer
    Cell & tissue research 280 (1995), S. 87-96 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Suprachiasmatic nucleus ; Catecholamines ; Dopamine ; Electron microscopy ; Vasoactive intestinal peptide ; Vasopressin ; Rat (Sprague Dawley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Catecholaminergic fibers in the suprachiasmatic nucleus of adult rats were investigated by use of light- and electron-microscopic immunocytochemistry. The suprachiasmatic nucleus receives a modest density of tyrosine hydroxylase-containing axons, homogeneously distributed in the nucleus and forming varicosities throughout its entire rostro-caudal extension. Immunolabeling with antibodies against dopamine showed that this catecholamine input comprises a dopaminergic component. Many tyrosine hydroxylase-positive cells were localized at the immediate periphery of the suprachiasmatic nucleus. With electron-microscopic examination, dendrites of these neurons were found within the limits of the nucleus as well as at a border zone between the suprachiasmatic nucleus proper and the optic tract where they received unlabeled synapses, providing a morphological support for a possible role of dopaminergic neurons in the integration and/or transfer of light-related signals. More than 91% of catecholaminergic axonal varicosities were found to establish morphologically defined synapses with dendrites. To investigate whether these synapses might be shared with neurons of one or both of the two main peptidergic populations of the nucleus, namely vasoactive intestinal peptide- and vasopressin-containing neurons, we carried out double-labeling experiments combining immunoperoxidase and immunogold-silver labeling. Results showed only a few cases of direct association of the catecholaminergic termin als with these peptidergic categories. In both types of dually stained sections, catecholaminergic synapses were preferentially made with unlabeled dendrites. The homogeneous distribution of tyrosine hydroxylase-immunoreactive fibers in the suprachiasmatic nucleus could therefore reflect a lack of significant catecholaminergic innervation of both vasoactive intestinal peptide- and vasopressin-synthesizing neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304514
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  • 41
    Electronic Resource
    Electronic Resource
    Morphological study of amelogenesis in the rat lower incisor after thyro-parathyroidectomy, parathyroidectomy and thyroidectomy (1995)
    Springer
    Cell & tissue research 283 (1995), S. 151-157 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Thyro-parathyroidectomy ; Parathyroidectomy ; Enamel formation ; Light microscopy ; Electron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The effects of thyro-parathyroidectomy, parathyroidectomy or thyroidectomy upon enamel formation in the rat incisor were studied. One control group and four groups of surgically treated rats were used: parathyroid autotransplanted, thyroidectomized, parathyroidectomized, and thyro-parathyroidectomized. One month after surgery, the incisors were processed for light and electron microscopy. The present study revealed perturbations of the Tomes’ process morphology, of the rod pattern in the inner enamel formation, of the enamel surface, and of the mineralization after thyro-parathyroidectomy. After parathyroidectomy, only mineralization defects could be visualised. No effects were observed in enamel after thyroidectomy. A severe hypocalcemic state as seen in thyro-parathyroidectomized rats affects the enamel shape, and mineralization, and the morphology and function of secretory ameloblasts. Knowledge of the way in which the alteration of the enamel surface is produced should contribute to a better understanding of the development of tooth enamel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050523
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  • 42
    Electronic Resource
    Electronic Resource
    Cranial meninges of goldfish: age-related changes in morphology of meningeal cells and accumulation of surfactant-like multilamellar bodies (1995)
    Springer
    Cell & tissue research 281 (1995), S. 349-358 
    Details
    ISSN: 1432-0878
    Keywords: Meninges ; Cerebrospinal fluid ; Electron microscopy ; Aging ; Multilamellar bodies ; Surfactant ; Goldfish ; Carassius auratus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In the optic tectum of goldfish, the outer, middle and inner layers of the endomeninx were evident in animals ranging in age from 1 month to several years. The outer layer in young animals consisted of closely overlapping cells with intertwined processes, whereas in the older animals it contained large extracellular spaces. The intermediate layer cells were always arranged in a single continuous layer, but in young animals they overlapped extensively with one another toward their edges whereas in the oldest animals they became extremely flat and non-overlapping. The inner layer included an outer tier of cells with their bases adhering to the intermediate layer, and an inner tier of cells detached from both the intermediate layer and the basal lamina overlying the brain parenchyma. Inner layer cells contained many large vacuoles that were in continuity with the extracellular space. With age, the extracellular space and the vacuolar system expanded, and the inner layer evolved into a meshwork of attenuated cytoplasmic processes embedded in the granular extracellular matrix. Another age-related feature was the accumulation adjacent to the basal lamina of uniform disc-shaped membranous structures, resembling multilamellar bodies of lung surfactant. These “disc bodies” were apparently generated by the coalescence of vesicles formed at the surface of the inner layer cells, possibly as a by-product of protein secretion by these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583403
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  • 43
    Electronic Resource
    Electronic Resource
    Do vasoactive intestinal peptide (VIP)- and nitric oxide synthase-immunoreactive terminals synapse exclusively with VIP cell bodies in the submucous plexus of the guinea-pig ileum? (1995)
    Springer
    Cell & tissue research 281 (1995), S. 485-491 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Nitric oxide synthase ; Vasoactive intestinal peptide ; Immunohistochemistry ; Electron microscopy ; Submucous plexus ; Guinea-pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In the submucous plexus of the guinea-pig ileum, previous light-microscopic studies have revealed that vasoactive intestinal peptide (VIP)-immunoreactive and nitric oxide synthase (NOS)-immunoreactive terminals are found predominantly in association with VIP-immunoreactive nerve cell bodies. In this study, double-label immunohistochemistry at the light-microscopic level demonstrated co-localization of NOS-immunoreactivity and VIP-immunoreactivity in axon terminals in submucous ganglia. About 90% of nerve fibres with NOS-immunoreactivity or VIP-immunoreactivity were immunoreactive for both antigens; only about 10% of labelled varicosities contained only NOS-immunoreactivity or VIP-immunoreactivity. The VIP/NOS varicosities were more often seen in the central parts of the ganglia, close to the VIP-immunoreactive cell bodies. Ultrastructural immunocytochemistry with antibodies to VIP was used to determine if NOS/VIP terminals synapse exclusively with VIP-immunoreactive nerve cell bodies. We examined the targets of VIP-immunoreactive boutons in two submucous ganglia from different animals. Serial ultrathin sections were taken through the ganglia after they had been processed for VIP immunocytochemistry. For each cell body, the number of VIP inputs (synapses and close contacts) was determined. The number of VIP-immunoreactive synapses received by the cell bodies of submucous neurons varied from 0–4 and the number of VIP-immunoreactive close contacts varied from 3–10. There was no significant difference between VIP-immunoreactive nerve cell bodies and non-VIP nerve cell bodies in the number of VIP-immunoreactive synapses and close contacts they received. Thus, the implication from light microscopy that NOS/VIP terminals end predominantly on VIP nerve cells was not vindicated by electron microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050443
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  • 44
    Electronic Resource
    Electronic Resource
    Localization of CD44, the hyaluronate receptor, on the plasma membrane of osteocytes and osteoclasts in rat tibiae (1995)
    Springer
    Cell & tissue research 280 (1995), S. 225-233 
    Details
    ISSN: 1432-0878
    Keywords: Key words: CD44 ; adhesion molecule ; Bone ; Osteoclasts ; Osteocytes ; Immunohistochemistry ; Confocal laser scanning microscopy ; Electron microscopy ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. CD44 is a multifunctional adhesion molecule that binds to hyaluronic acid, type I collagen, and fibronectin. We have studied the immunohistochemical localization of CD44 in bone cells by confocal laser scanning microscopy and transmission electron microscopy in order to clarify its role in the cell-cell and/or cell-matrix interaction of bone cells. In round osteoblasts attached to bone surfaces, immunoreactivity is restricted to their cytoplasmic processes. On the other hand, osteocytes in bone matrices show intense immunoreactivity on their plasma membrane. Intense immunoreactivity for CD44 can be detected on the basolateral plasma membranes of osteoclasts. There is considerably less reactivity observed in the area of the plasma membrane that is in direct contact with bone. The pre-embedding electron-microscopical method has revealed that CD44 is mainly localized on the basolateral plasma membrane of osteoclasts. However, the ruffled border and clear zone show little immunoreactivity. A CD44-positive reaction can be detected on both plasma membranes in the contact region between osteoclasts and osteocytes. These findings suggest that: 1) cells of the osteoblast lineage express CD44 in accordance with their morphological changes from osteoblasts into osteocytes; 2) osteoclasts express CD44 on their basolateral plasma membrane; 3) CD44 in osteoclasts and osteocytes may play an important role in cell-cell and/or cell-matrix attachment via extracellular matrices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307793
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  • 45
    Electronic Resource
    Electronic Resource
    Ultrastructural investigation of nitric oxide synthase-immunoreactive nerves associated with coronary blood vessels of rat and guinea-pig (1995)
    Springer
    Cell & tissue research 280 (1995), S. 575-582 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Nitric oxide synthase ; Coronary vasculature ; Electron microscopy ; Immunocytochemistry ; Rat (Sprague Dawley) ; Guinea-pig (Dunkin Hartley)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Ultrastructural investigation of nitric oxide synthase-immunoreactive nerves closely associated with blood vessels in rat and guinea-pig hearts revealed many labelled nerve fibres in the walls of the main branches of the coronary arteries, and in arterioles, capillaries and post-capillary venules. The number of nitric oxide synthase-containing nerve fibres associated with different vessels, even those of the same calibre, varied. Terminal regions of nitric oxide synthase-immunoreactive fibres were observed in the endocardium and myocardium. Nitric oxide synthase-labelled fibres displayed electron-dense immunoproduct in both varicose and intervaricose regions. Immunoreactive axonal varicosities contained both small and large synaptic vesicles. The characteristics of the nitric oxide synthase-immunoreactive nerve fibres observed in the heart and the possibility that these fibres represent the processes of intracardiac neurones and/or sensory neurones of extrinsic origin are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318361
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  • 46
    Electronic Resource
    Electronic Resource
    Ultrastructural study of periodic lamellar granules in human neutrophils (1995)
    Springer
    Cell & tissue research 281 (1995), S. 69-76 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Periodic lamellar granule ; Primary granule ; Chronic myeloproliferative disorder ; Neutrophils ; Electron microscopy ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Granules consisting of periodically arranged membranous lamellae and amorphous electron-opaque material, i.e., periodic lamellar granules, are present in human neutrophils. To date, no extensive ultrastructural studies have been carried out on these granules because of their infrequent presence in neutrophils. The bone marrow of 18 cases of chronic myeloproliferative disorders, including one case of chronic neutrophilic leukemia in which periodic lamellar granules were frequently seen in neutrophils, was investigated by electron microscopy. Periodic lamellar granules were seen in neutrophils in 12 of the 18 cases at varying frequencies. They were preferentially seen in immature neutrophils. The transverse profiles of these granules revealed concentric complete/incomplete rings or periodic parallel straight lines, i.e., various patterns of lamellar arrangement were present. Periodic lamellar granules were positive for myeloperoxidase and lysozyme at the electron-microscopic level. These results suggest that these granules represent a primary neutrophil granule subtype. However, their functional and pathologic significance remains unknown.
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    URL: http://dx.doi.org/10.1007/BF00307959
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  • 47
    Electronic Resource
    Electronic Resource
    Immunocytochemical colocalization of adhesive proteins with clathrin in human blood platelets: further evidence for coated vesicle-mediated transport of von Willebrand factor, fibrinogen and fibronectin (1995)
    Springer
    Cell & tissue research 279 (1995), S. 453-457 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Blood platelets ; Immunocytochemistry ; Electron microscopy ; Coated vesicles ; Clathrin ; Adhesive proteins ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Coated membranes and vesicles play an important role in receptor-mediated endocytosis and intracellular trafficking in various cell types, and are also present in blood platelets. Platelets take up certain proteins from the blood plasma, such as von Willebrand factor and fibrinogen, and these substances are transferred to storage granules. The receptors for these plasma proteins on the platelet plasma membrane have been well characterized, but morphological evidence for their transport to the storage granules is not yet available. In an attempt to clarify this aspect, we employed postembedding immunocytochemistry on platelets embedded in the acrylic resin LR White. Clathrin as the major coat component of coated vesicles was localized in the cytoplasm, on the plasmic faces of α-granules and the open canalicular system, and on the plasmic face of the plasma membrane. Colocalizations of the adhesive proteins, von Willebrand factor, fibrinogen and fibronectin, with clathrin could be observed at the same typical locations as coated vesicles were seen in Araldite-embedded material. These colocalizations have not been reported to date and furnish further evidence for a coated vesicle-mediated transport of blood plasma-derived adhesive proteins from their receptors on the outer plasma membrane to the α-granules.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050304
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  • 48
    Electronic Resource
    Electronic Resource
    Cranial meninges of goldfish: age-related changes in morphology of meningeal cells and accumulation of surfactant-like multilamellar bodies (1995)
    Springer
    Cell & tissue research 281 (1995), S. 349-358 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Meninges ; Cerebrospinal fluid ; Electron microscopy ; Aging ; Multilamellar bodies ; Surfactant ; Goldfish ; Carassius auratus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. In the optic tectum of goldfish, the outer, middle and inner layers of the endomeninx were evident in animals ranging in age from 1 month to several years. The outer layer in young animals consisted of closely overlapping cells with intertwined processes, whereas in the older animals it contained large extracellular spaces. The intermediate layer cells were always arranged in a single continuous layer, but in young animals they overlapped extensively with one another toward their edges whereas in the oldest animals they became extremely flat and non-overlapping. The inner layer included an outer tier of cells with their bases adhering to the intermediate layer, and an inner tier of cells detached from both the intermediate layer and the basal lamina overlying the brain parenchyma. Inner layer cells contained many large vacuoles that were in continuity with the extracellular space. With age, the extracellular space and the vacuolar system expanded, and the inner layer evolved into a meshwork of attenuated cytoplasmic processes embedded in the granular extracellular matrix. Another age-related feature was the accumulation adjacent to the basal lamina of uniform disc-shaped membranous structures, resembling multilamellar bodies of lung surfactant. These ”disc bodies” were apparently generated by the coalescence of vesicles formed at the surface of the inner layer cells, possibly as a by-product of protein secretion by these cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00583403
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  • 49
    Electronic Resource
    Electronic Resource
    Immunogold labelling of estradiol receptor in MCF7 cells (1995)
    Springer
    Cell & tissue research 279 (1995), S. 445-452 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Estradiol receptor ; Breast cancer cells ; Cell culture ; Ultrastructure ; Electron microscopy ; Immunohistochemistry ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an es- tradiol-induced translocation of this ligand-dependent transcription regulator.
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    URL: http://dx.doi.org/10.1007/s004410050303
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  • 50
    Electronic Resource
    Electronic Resource
    Immunogold labelling of estradiol receptor in MCF7 cells (1995)
    Springer
    Cell & tissue research 279 (1995), S. 445-452 
    Details
    ISSN: 1432-0878
    Keywords: Estradiol receptor ; Breast cancer cells ; Cell culture ; Ultrastructure ; Electron microscopy ; Immunohistochemistry ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The distribution of estradiol receptor in serial sections of estradiol-deprived and estradiol-stimulated MCF7 cells was studied by using mouse monoclonal antibodies reacting with different domains of the receptor and goat-antimouse IgG/6 nm gold. In the nucleus and the cytoplasm of estradiol-deprived cells, the receptor was detected by all three monoclonals (13H2, HT 65 and MA1-310). The antibodies 13H2 and MA1-310 detected receptor associated to the microfilament bundles in the cytoplasm. Higher densities of antireceptor attachment to the nuclear areas were accompanied by a reduction in the attachment to the cytoplasm after estradiol stimulation of the cells. The results confirm earlier observations on the presence of cytoplasmic estrogen receptor in estradiol-deprived cells and support the premise of an estradiol-induced translocation of this ligand-dependent transcription regulator.
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    URL: http://dx.doi.org/10.1007/BF00318156
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  • 51
    Electronic Resource
    Electronic Resource
    Immunocytochemical colocalization of adhesive proteins with clathrin in human blood platelets: further evidence for coated vesicle-mediated transport of von Willebrand factor, fibrinogen and fibronectin (1995)
    Springer
    Cell & tissue research 279 (1995), S. 453-457 
    Details
    ISSN: 1432-0878
    Keywords: Blood platelets ; Immunocytochemistry ; Electron microscopy ; Coated vesicles ; Clathrin ; Adhesive proteins ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Coated membranes and vesicles play an important role in receptor-mediated endocytosis and intracellular trafficking in various cell types, and are also present in blood platelets. Platelets take up certain proteins from the blood plasma, such as von Willebrand factor and fibrinogen, and these substances are transferred to storage granules. The receptors for these plasma proteins on the platelet plasma membrane have been well characterized, but morphological evidence for their transport to the storage granules is not yet available. In an attempt to clarify this aspect, we employed postembedding immunocytochemistry on platelets embedded in the acrylic resin LR White. Clathrin as the major coat component of coated vesicles was localized in the cytoplasm, on the plasmic faces of α-granules and the open canalicular system, and on the plasmic face of the plasma membrane. Colocalizations of the adhesive proteins, von Willebrand factor, fibrinogen and fibronectin, with clathrin could be observed at the same typical locations as coated vesicles were seen in Araldite-embedded material. These colocalizations have not been reported to date and furnish further evidence for a coated vesicle-mediated transport of blood plasma-derived adhesive proteins from their receptors on the outer plasma membrane to the α-granules.
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    URL: http://dx.doi.org/10.1007/BF00318157
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  • 52
    Electronic Resource
    Electronic Resource
    Investigation of the structural and functional features of splenic ellipsoids in rainbow trout (Oncorhynchus mykiss) (1995)
    Springer
    Cell & tissue research 279 (1995), S. 469-474 
    Details
    ISSN: 1432-0878
    Keywords: Ellipsoids ; Spleen ; Electron microscopy ; Fluorescent microspheres ; Oncorhynchus mykiss (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The ultrastructure of ellipsoids in the spleen of rainbow trout (Oncorhynchus mykiss) is described. The endothelium of terminations of arterioles bulged into the lumen, and gaps between the endothelial cells were evident. A continuous basal lamina was not present, and there were extensive interdigitations between the endothelial cells and surrounding reticular cells. The interdigitating processes were rich in microfilaments. Intravenously injected colloidal carbon, approximately 0.03 μm in diameter, was held in the reticular matrix of the ellipsoidal wall and taken up by macrophages that extended cellular processes among the reticular and endothelial cells. The intravenous injection of fluorescent polystyrene microspheres of known diameter showed that microspheres with a diameter of 0.5 or 1.0 μm were localised in the red pulp, whereas microspheres with a diameter of 0.15 μm were retained in ellipsoidal walls. Thus, the terminations of splenic arterioles in rainbow trout were found to be consistent with descriptions of ellipsoids in other vertebrates in that they possessed a speciallised cuboidal endothelium, lacked a continuous basal lamina, were surrounded by a sheath of macrophages and reticular cells, and had a sheath of macrophages and reticular cells, and had a role in the selective filtration and retention of bloodborne particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318160
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  • 53
    Electronic Resource
    Electronic Resource
    Electron-microscopic localisation of thiol and disulphide groups by direct monomaleimido-nanogold labelling in the spermatozoa of a marsupial, the tammar wallaby (Macropus eugenii) (1995)
    Springer
    Cell & tissue research 282 (1995), S. 291-296 
    Details
    ISSN: 1432-0878
    Keywords: Spermatozoa ; Acrosome ; Thiols ; Disulphides ; Electron microscopy ; Monomaleimido nanogold ; Macropus eugenii (Marsupialia)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract This study utilised a commercially available monomaleimido-nanogold reagent to directly label cellular thiol groups (SH) of marsupial (tammar wallaby) spermatozoa before and after reduction of disulphides (S-S) with mercaptoethylamine hydrochloride (MEA). The sperm surface, mitochondrial membranes, axoneme and tail fibres were all labelled with gold particles before MEA treatment and the label intensity was increased after S-S reduction. The acrosomal membranes and matrix of spermatozoa contained no detectable SH prior to MEA treatment. However, after moderate MEA treatment (1 mg/ml) gold label was associated with the acrosomal membrane and invaginated acrosomal membrane within the acrosomal matrix. After exposure to 5 and 10 mg/ml MEA, gold particles heavily labelled the acrosomal matrix. Thus, the acrosomal membranes and matrix of tammar wallaby spermatozoa both contain S-S cross-linked structures, and this may contribute to the unusual stability of the marsupial acrosome. Under all treatment conditions the nucleus remained unlabelled. This is consistent with early studies which indicated that cysteine was absent from the nuclear protamines. The study also demonstrated that monomaleimido-nanogold can be used to resolve SH- and S-S-rich cellular structures directly, in addition to its use to label antibodies and Fab fragments for immunochemical localisation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00319119
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    Dynamics of secretory granules in somatotrophs of rats after stimulation with growth hormone-releasing factor: a stereological analysis (1995)
    Springer
    Cell & tissue research 282 (1995), S. 493-501 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Somatotrophs ; Growth hormone ; Growth hormone-releasing factor ; Secretory granule ; Electron microscopy ; Morphometry ; Rat (Fischer 344)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The anterior pituitary tissue of male rats injected with growth hormone-releasing factor (GRF) was either processed for stereology at the light- and electron-microscopic levels, or homogenized for growth hormone (GH) assay 2–60 min after GRF injection. Secretory granules of somatotrophs became smaller but increased in numerical density 2 min after GRF injection. Their volume density began to increase at 5 min. The frequency of exocytosis of the granules was most prominent as early as 2 min after GRF injection and reduced thereafter. GH levels in the tissue were lowest at 2–5 min, and returned to the control value by 60 min. Serum GH levels were highest at 15 min; even at 60 min, this value was higher than in the controls. These findings suggest that secretory granules in somatotrophs are stimulated to divide by GRF, resulting in a decrease in size and an increase in number. The discrepancy between the earlier formation of new secretory granules and the later restoration of intracellular GH levels implies that GRF first stimulates the synthesis of constituents of granules other than GH, and only later the synthesis of GH, and that newly formed small secretory granules contain less GH. From the clearance rate of serum GH and the frequency of granule exocytosis, it can be estimated that about a half million granules are released to maintain 1 ng/ml of serum GH in rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050500
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    A comparative electron microscopic study of chemical techniques for preservation of auditory ossicles as homografts (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 239-243 
    Details
    ISSN: 1434-4726
    Keywords: Homograft auditory ossicles ; Preservative solutions ; Shelf life ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The ultrastructure of preserved ossicles at different shelf lives was compared. Homograft ossicles were harvested and preserved in 70% alcohol, 1:5000 Cialit (sodium-2-ethyl-mercuri-mercapto-benzoxazole-5-carboxylate), and buffered 4% formaldehyde at pH 7. The ultrastructure of each ossicle was studied by both scanning and transmission electron microscopy and was compared to that of fresh controls placed in 0.9 N saline solution. The results indicated clearly the superiority of 70% alcohol as a long-term preservative and 1:5000 Cialit as a short-term preservative.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00179918
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    Morphological studies on the pathogenesis of Reinke's edema (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 469-474 
    Details
    ISSN: 1434-4726
    Keywords: Reinke's laryngeal edema ; Electron microscopy ; Immunohistochemistry ; Pathogenesis Neobursa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Light microscopy of vocal cord mucosa in patients with Reinke's edema revealed highly ramified fissured spaces in the subepithelial tissue that were generally lined with flat cells. The ultrastructure of the parietal cells resembled fibroblasts whose cytoplasmic extensions overlapped in two to three layers in some places. Cell contacts were not observed. Neither electron microscopy nor immunohistochemical testing with antibody against laminin demonstrated a basal membrane. It was possible to distinguish between light and dark cells in the specimens examined. The cytoplasm of the light cells contained intermediate filaments, mitochondria, lysosomes, coated vesicles, caveolae and broad cisternae of rough endoplasmic reticulum. The dark cells were more numerous and typically exhibited a well-developed endoplasmic reticulum and free ribosomes. The parietal cells showed no immunoreaction against human vascular endothelial cells. Immunohistochemical demonstration of mesenchymal intermediate filaments using antibody against vimentin yielded a positive reaction for some of the cells in the walls of the crevices and subepithelial tissue. It was also possible to demonstrate a few cells with monoclonal antibody against macrophages (KiM6). These findings contradict the concept of lymphatic distension in cases of Reinke's edema. Since the parietal cells seen resembled synoviocytes in their structure and immunohistochemical reactions, findings indicate that the hollow spaces of Reinke's edema develop like neobursae from mechanical strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02114753
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    Immunohistochemical and fine structural characterization of primary carcinoid tumors of the larynx (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 229-235 
    Details
    ISSN: 1434-4726
    Keywords: Larynx ; Neuroendocrine carcinoma ; Carcinoid tumor ; Immunohistochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Carcinoid tumors belong to the group of neuroendocrine tumors of epithelial origin, i.e., neuroendocrine carcinomas. These neoplasms usually occur in the gastrointestinal tract or bronchial system but are very rare neoplasms in the larynx. Since carcinoid tumors in this latter site may appear to be undifferentiated by light microscopy, they may possibly be misinterpreted and their neuroendocrine characteristics may remain unrecognized. Using immunohistochemical methods, three carcinoid tumors were studied and showed positive immunostaining for markers of epithelial origin (cytokeratins, epithelial membrane antigen, carcino-embryonic antigen) and, in particular, for markers of neuroendocrine differentiation (chromogranin, synaptophysin, neuron-specific enolase). All tumors expressed calcitonin-, serotonin- and adrenocorticotropic-hormone-like immunoreactivity. In contrast, three poorly differentiated squamous cell carcinomas showed positive immunostaining for epithelial markers but did not show any immunoreactivity with markers of endocrine characteristics. Fine structurally, carcinoid tumor cells contained neurosecretory-type granules scattered throughout the cytoplasm. The present study demonstrated that (1) carcinoid tumors of the larynx possess distinct immunohistochemical characteristics that allow a clear classification, (2) it is advisable to use a battery of primary antibodies rather than rely on specificity and sensitivity of a single marker to establish diagnosis and (3) the fine structural demonstration of neurosecretory-type granules serves as a reliable adjunct to diagnosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00179916
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    Recurrent amyloid tumor of the parotid gland (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 53-56 
    Details
    ISSN: 1434-4726
    Keywords: Parotid gland ; Amyloidosis ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A case of an organ-limited amyloid tumor of the left parotid gland is described with a history of recurrence. A slowly growing parotid mass was the only symptom. After 5.5 years following local excision, the patient was readmitted with a slowly growing recurrence in the superficial lobe of the previously treated gland. Lateral parotidectomy was performed with wide excision of the infiltrated tissue and preservation of the facial nerve. Primary amyloidosis of the AL type was confirmed with immunohistochemical studies revealing staining for lambda but not kappa light chains of immunoglobulins. There has been no clinical or laboratory evidence of systemic amyloidosis or recurrence after 2 years. To the best of our knowledge, this is the first report of a recurrent amyloid tumor of the parotid gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00171441
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    Cell-surface glycoconjugate layer in the tubotympanic mucosa of the guinea pig as revealed by wheat germ agglutinin/gold labelling (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 249-254 
    Details
    ISSN: 1434-4726
    Keywords: Middle ear ; Glycoconjugate ; Wheat germ agglutinin ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the protective function of the mucous blanket (MB) against lectin substances, we examined at the ultrastructural level whether intraluminal colloidal gold-labelled wheat germ agglutinin (WGA) could enter the MB-covered epithelial cell surface of the guinea pig tubotympanic mucosa. Post-embedding staining with WGA/gold on thin tissue sections was done in parallel for comparison. The cell surface glycoconjugate of the eustachian tubal and transitional epithelium had a typical bilayered structure: the outer MB and the microvilli-associated glycocalyx (MAG), which were interposed by the interciliary fluid zone. In squamous epithelium of the distal middle ear, the MB adhered to the MAG, thereby forming a monolayered coat of glycoconjugates at the cell surface. In the pre-embedding staining, WGA/gold did not bind with the MB and MAG in the eustachian tube, and exclusively bound with MB in the transitional area. Direct binding was also found with MAG and the apical plasmic membrane in the squamous epithelium. These findings indicate that MAG is occluded by MB lined with the interciliary fluid zone for luminal access of lectin at the proximal lumina of the tubotympanic epithelium. It is also suggested that MB existing at two sites possesses a different WGA-binding capacity: shielding as a “dust cover” in the eustachian tube and entrapping as a “flypaper” against lectin in the transitional area of the middle ear.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00179920
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    Morphology and evolutionary implications of the annual cycle of secretion and sperm storage in spermathecae of the salamander Ambystoma opacum (Amphibia: Ambystomatidae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 35-46 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Females of the marbled salamander, Ambystoma opacum, store sperm in exocrine glands called spermathecae in the roof of the cloaca. Eggs are fertilized by sperm released from the spermathecae during oviposition. Some sperm remain in the spermathecae following oviposition, but these sperm degenerate within a month and none persists more than 6 mo after oviposition. Thus, sperm storage between successive breeding seasons does not occur. Apical secretory vaculoes are abundant during the fall mating season and contain a substance that is alcian blue+ at pH 2.5. Production of secretory vacuoles decreases markedly after oviposition, and the glands are inactive by the summer months. Ambystoma opacum is a terrestrial breeder, and some mating occurs prior to arrival at pond basins where oviposition occurs. Mating prior to arrival at the ovipository site may prolong the breeding season, leading to fitness implications for both males and females. Females have opportunities for more matings, and the possibilities for sperm competition in the spermathecae are enhanced. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230106
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    Annual changes in fine structure of inner epithelial lining of the ovary of a marine sculpin, Alcichthys alcicornis (Teleostei: Scorpaeniformes), with internal gametic association (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 85-97 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Alcichthys alciocornis has a viscous ovarian fluid in the ovarian cavity, which plays an important role in its unique mode of reproduction called internal gametic association (i.e., internal insemination and sperm-egg association but a delay in the physiological fertilization until spawning). Seasonal changes in fine structure of the inner epithelial lining and capillary endothelium of the ovary revealed that ovarian fluid originated as a result of the secretory activity of the tissues. The ovarian cavity of A. alcicornis is lined with an ovigerous lamella epithelium and an ovarian wall epithelium. During the spawning period, both epithelia actively secreted proteinaceous substances which seemed to constitute the ovarian fluid. The substances appear to be synthesized in the rough-surfaced endoplasmic reticulum from the material which was transported from the blood capillary, taken into the epithelial cells by endocytosis, accumulated in secretory vesicles via Golgi apparatus in the cells, and finally released into the ovarian cavity by exocytosis. Microapocrine secretion was also observed to occur in both epithelia. Secretory activity of both epithelia by exocytosis and microapocrine secretion showed distinct seasonal changes. Active exocytosis and microapocrine secretion were observed during the spawning period (April-May). These activities slightly declined during the degeneration period (May-June) and were lost during the early recovery period (July). During the mid to late recovery period (October-March), there was some exocytosis but no microapocrine secretion. © 1995 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230108
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    Surface morphology of hamster (Mesocricetus auratus) decidual cells in vitro (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 167-174 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cell surface morphology of hamster decidual cells isolated from day 8 implantation swellings was studied, using both phase-contrast and scanning electron microscopy. Two kinds of cells, fibroblastic and epithelioid, were identified in cultures examined by phase-contrast microscopy. Fibroblastic cells were spindle-shaped, having pointed or blunt terminals on one end and bifid or webbed projections at the other end. Epithelioid cells, on the other hand, were flat and discoid, having a distinctively ruffled plasma membrane. Further, the plasma membrane of epithelioid cells formed rope-like or flange-like processes. The significance of such adaptations is discussed. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230205
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    Skeletal morphogenesis in the urodele skull: II. Effect of developmental stage in thyroid hormone-induced remodeling (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 149-166 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study investigates the effect of developmental stage on thyroid hormone (TH)-mediated remodeling in the skeletal tissues of hemidactyliine plethodontid urodeles. Rate of morphogenesis was quantified in 17 metamorphic tissues for three different size-age classes of Eurycea bislineata larvae immersed in a metamorphic dosage of T4. Extent of morphogenesis after a 3-week immersion was also quantified in these tissues plus four larval ones for the full size range of E. bislineata larvae and for less complete size ranges of E. wilderae, E. longicauda guttolineata, Gyrinophilus porphyriticus, and Pseudotriton ruber larvae. Although all tissues respond more slowly with decreasing size/age, two tissue-specific effects are evident in all species. Larval ossifications are less inducible than metamorphic ossifications, and progressive metamorphic events are more retarded and, in some cases, more prone to abnormal morphogenesis than regressive ones. The first effect agrees with the prediction that tissues that naturally remodel at metamorphosis are more responsive to a metamorphic dosage of TH than those that respond at a larval stage and lower TH. The second effect agrees with the prediction that progressive morphogenesis is more likely to be impaired at small size than regressive morphogenesis, although the frequent discrepancies between individuals of similar size implicate developmental age more than size in this effect. Collectively, these two effects provide only equivocal support for the hypothesis that direct development in plethodontids evolved via precocious TH activity. However, the unexpected transition from ceratobranchial replacement to ceratobranchial shortening in medium-sized larvae suggests that the former pathway requires a longer period of cell specification at low TH. Since ancestral plethodontids appear to have been distinguished by an exceptionally long larval period with exceptionally low TH activity, this developmental prerequisite may in turn be partly responsible for their singular evolution of ceratobranchial replacement. © 1995 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230204
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    Atomic force microscopy of the morphology of the matrix and mineral components of the otolith of Hyperoglyphe antarctica (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 203-214 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The sagittal otolith of Hyperoglyphe antarctica (Centrolophidae: Teleostei) has a prismatic structure in which the anti-sulcal growth axes of each prism consist of a series of nested cones each composed of a mineral layer followed by an organic matrix layer. Broken sections show the mineral layers to be composed of stacks of crystals. Otolith matrix that has been decalcified and air-dried, or critical-pont-dried, retains a periodic structure of repeating high and low matrix density. At high magnifications, both broken whole crystal surfaces and decalcified matrix surfaces have a granular structure. Chloroxbleached whole otoliths also show a granular crystalline structure. At higher magnifications, the air-dried matrix showed a parallel fiber structure with similar dimensions to keratin fibers. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230208
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    Cellular ultrastructure and catecholamine histofluorescence of the heart of the Australian lungfish, Neoceratodus forsteri (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 191-201 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ultrastructural descriptions of the dipnoan heart are lacking. Many ultrastructural features of the heart of the Australian lungfish, Neoceratodus forsteri, resemble those of other lower vertebrates. The epicardial cells appear to be adapated for the exchange of material with the pericardial fluid. The most prominent features of the endocardial cells are numerous moderately electron-dense vesicles found within the cytoplasm. These organelles might have an endocrine function. The myocardiocytes are typically small. The banding pattern of the sarcomere is shared with most fish. The intercalated disc has a convoluted path and consists of desmosomes and fascia adherens. Caveolae are a prominent feature of the sarcoplasm. The sarcoplasmic reticulum is sparse, and T-tubules are lacking. Atrial myocardial dense bodies occur in vast numbers throughout the atrium and are occasionally seen in the ventricle. These vesicles are chromaffin-positive but fail to show catecholamine fluorescence. They are likely to contain peptides related to ANP. Subendothelial cells exhibiting catecholamine-specific fluorescence are scattered throughout the atrium. Ultrastructurally these cells contain many chromaffin-positive granules. Chromaffin cells represent another cell type with a probable endocrine function within the heart of N. forsteri. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052230207
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    Masthead (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052230301
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    Morphology of complex lymphoepithelial organs of the anal canal (“anal tonsil”) in the bottlenose dolphin, Tursiops truncatus (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 263-268 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A complex of lymphoepithelial organs, the “anal tonsils,” is a consistent structure in the anal canal of the bottlenose dolphin, Tursiops truncatus. This complex occurs as a circumferential cluster of discrete tonsil like aggregations of lymphoid tissues, together with epithelial ducts (“crypts”) and occasional mucus secretory units in the extreme lower portion of the intestinal tract. These structures are concentrated in the segment lined by stratified squamous epithelium and extend for a variable distance cephalad from the anal aperture. The tonsils appear to be most active, judged by the amount of lymphoid tissue present, in young animals. Depletion of lymphocytes and cystic enlargement of the crypts, probably representing functional as well as morphological involution, is a consistent feature of older animals. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230304
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    Prenatal development of the integument in Delphinidae (Cetacea: Odontoceti) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 269-287 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The prenatal development of epidermis, dermis, and hypodermis was studied in embryos of different ago of two delphinid species (Stenella attenuata, Delphinus delphis), using light and transmission electron microscopical methods. The delphinid embryo is covered by a multilayered tissue formed by four different epidermal generations (periderm, stratum intermedium-I, str. intermedium-II, str. spinosum) produced by the str. basale. The first layer appears at about 40-50 mm of body length, the second type (s.i.-I) about 60-160 mm, and the third type (s.i.-II) is present at 160-500 mm. The first spinosal cells are produced at 225-260 mm body length; thenceforth, the epidermis increases continuously in thickness. Epidermal ridge formation begins about 400-mm body length. The development of the dermis is characterized by the early production of thin connective tissue fibers (40- 70-mm body length) and simultaneously the cutaneuous muscle matures in structure. Vascular development intensifies between embryos of 150-225 mm, and collagen production increases markedly in fetuses of 225-260-mm length. These events are paralledled by an increase in dermal thickness. The first elastic fibers can be recognized in the skin from the abdomen at about 600-mm body length. The development of the hypodermis is marked by very rapid and constantly progressing growth, beginning about 60-mm body length. The first typical fat cells appear in animals of 360-400 mm. Regional differences are obvious for all skin layers with regard to the flippers, where structural maturation proceeds more rapidly than in dorsal or abdominal regions. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230305
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    Design of the predatory legs of water bugs (Hemiptera: Nepidae, Naucoridae, Notonectidae, Gerridae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 289-302 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Functional comparative morphology of predatory legs in five species of water bugs (Ilyocoris cimicoides, Nepa cinerea, Ranatra linearis, Notonecta glauca, and Gerris lacustris) has been investigatd adn the following peculiarities of leg design were revealed.1Subcoxal articulation may be monoaxial (G. lacustris, N. glauca), or, in contrast to walking leg type, biaxial (N. cinerea, R. linearis, I. cimicoides); the first axis is oriented along the coxa (torsion axis), the second one is perpendicular to the first (non-torsion axis).2In contrast to walking leg type, which is characterized by cross suspension of the axis of coxal rotation in thoracal skeleton, this axis in G. lacustris is placed vertically. Non-torsion coxal axis in R. linearis is oriented strongly transversal. This axis directs the leg strike forward.3Legs in the majority of species are planar: Torsion axes of the coxa, femur, and tibia are placed in the same plane. Axes of rotation of consequent joints in I. cimicoides are reciprocally sloped. Therefore, the end of the leg outlines the spiral trajectory, when all angles of joints are opening (closing). This is an adaptation for clinging to the stems of water plants.4Passive adduction of the femur in the trochanter-femoral joint in N. glauca allows it to go around protuberances of the body wall, when the leg is sliding along them; recurrent femur movement during releasing from the obstacele is active due to the rt.fe muscle.5Only R. linearis has predatory legs, which permit the high-speed pursuit of potential prey; other species realize this function using the swimming legs, whereas the forelegs are used for the manipulation movements.6Muscle arrangement in the prothorax of different species reflects both leg construction and constructional constraints of body design. Powerful flexor muscles (co1, co2, co3, co5, fl.ti, et.ti in R. linearis; fl.ta, fl.ti in N. glauca; fl.ti in I. cimicoides) have long tendons and short muscle bundles, which originate on the leg wall. As a result, the powerful force is developed along the muscle tendon.7Some features of the predatory leg are common for the species studies: elongation of coxae, thickening of femora, and increase of the degree of junction of tibia and tarsus. The muscles, which move the distal segment of the leg, are reinforced and the sclerite of the fl.ti tendon is enlarged. The joint angle of the distal segment is increased to 120°. © 1995 Wiley-Liss, Inc.
    Additional Material: 27 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230306
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    Allometry and developmental integration of body growth in a piranha, Pygocentrus nattereri (Teleostei: Ostariophysi) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 341-355 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Piranhas, like many teleosts, change their diets on both ontogenetic and phylogenetic time scales. Prior studies have suggested that pervasive morphological changes in body form on a phylogenetic time scale may be related to changes in diet, but previous reports have found little shape change in piranhas on an ontogenetic time scale. We re-examine the post-transformational allometry of body form in one piranha, Pygocentrus nattereri (Kner), using the method of thin-plate splines decomposed by their partial warps. We find substantial evidence of allometry, primarily elongation of the mid-body relative to the more anterior and posterior regions, elongation of the postorbital and nape regions relative to the more anterior head and posterior body, and deepening of the head relative to the body. In addition to these pervasive changes throughout the body, there are some that are more localized, especially elongation of the postorbital region relative to eye diameter and snout, and an even more localized elongation of the snout relative to eye diameter. Initial dietary transitions are associated with changes in head and jaw proportions, but rates of shape change decelerate through growth, so that the final transition to a diet increasingly dominated by small whole fish appears associated with change largely in overall body size. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230309
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    Ultrastructurally different muscle cell types in Eisenia foetida (Annelida, Oligochaeta) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995), S. 87-96 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Muscles in the body wall, intestinal wall, and contractile hemolymphatic vessels (pseudohearts) of an oligochaete anelid (Eisenia foetida) were studied by electron microscopy. The muscle cells in all locations, except for the outer layer of the pseudohearts, are variants of obliquely striated muscle cells. Cells comprising the circular layer of the body wall possess single, peripherally located myofibrils that occupy most of the cytoplasm and surround other cytoplasmic organelles. The nuclei of the cells lie peripherally to the myofibrils. The sarcomeres consist of thin and thick myofilaments that are arranged in parallel arrays. In one plane of view, the filaments appear to be oriented obliquely to Z bands. Thin myofilaments measure 5-6 nm in diameter. Thick myofilaments are fusiform in shape and their width decreases from their centers (40-45 nm) to their tips (23-25 nm). The thin/thick filament ratio in the A bands is 10. The Z bands consist of Z bars alternating with tubules of the sarcoplasmic reticulum. Subsarcolemmal electron-dense plaques are found frequently. The cells forming the longitudinal layer of the body wall musculature are smaller than the cells in the circular layer and their thick filaments are smaller (31-33 nm centrally and 21-23 nm at the tips). Subsarcolemmal plaques are less numerous. The cells forming the heart wall inner layer, the large hemolymphatic vessels, and the intestinal wall are characterized by their large thick myofilaments (50-52 nm centrally and 27-28 nm at the tips) and abundance of mitochondria. The cells forming the outer muscular layer of the pseudohearts are smooth muscle cells. These cells are richer in thick filaments than vertebrate smooth muscle cells. They differ from obliquely striated muscle cells by possessing irregularly distributed electron-dense bodies for filament anchorage rather than sarcomeres and Z bands and by displaying tubules of smooth endoplasmic reticulum among the bundles of myofilaments. © 1995 Wiley-Liss, Inc.
    Additional Material: 21 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052240110
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    Internal fertilization, testis and sperm morphology in glandulocaudinae fishes (Teleostei: Characidae: Glandulocaudinae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995), S. 131-145 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this report, the gonads of 32 glandulocaudine species, representing 18 genera, are compared with 11 outgroup characiform species. Through the presence of spermatozoa within the ovarian cavity, internal fertilization of the female is confirmed for the 16 genera for which mature ovaries were available. No outgroup ovary studied contains spermatozoa. All mature glandulocaudine testes have a large portion of the posterior testis, which is devoid of developing germ cells and spermatocysts (aspermatogenic), devoted to sperm storage, with the degree of partitioning in that region varying greatly within the group. All outgroup species examined have spermatozoa with spherical nuclei. With the exception of the species of the genus Planaltina, which also have spherical nuclei, all glandulocaudines have elongated nuclei, which vary among the species from 3.6 μm to 31.6 μm in length. Distinct sperm packets (spermatozeugmata) are formed in five genera by two different methods. In the genera Xenurobrycon, Tyttocharax, and Scopaeocharax, all of the tribe Xenurobryconini, the spermatozeugmata are formed within the spermatocysts and released fully formed. In all genera of the tribe Glandulocaudini, which includes Glandulocauda and Mimagoniates, loose spermatozoa are released which cluster into spermatozeugmata within the posterior storage areas. These morphological specializations are discussed within a phylogenetic framework as adaptations for internal fertilization and are hypothesized to be independently derived. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052240203
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    Presence of interdigitating cells in thymus of the sea bass, Dicentrarchus labrax L. (Teleost) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995), S. 199-203 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Interdigitating cells in the thymus of the sea bass, Dicentrarchus labrax, occur principally in the internal zone and in the border with the external zone. Ultrastructurally, the most characteristic cytological features of these cells are their low electron density, complicated labyrinthine membrane-membrane contacts, scantiness of cytoplasmic organelles, presence of Birbeck-like granules, juxtanuclear tubulo-vesicular complex, and phagocytic capacity. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052240208
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    Morphological, histochemical, and ultrastructural characterization of the salivary glands and proboscises of three species of glossiphoniid leeches (Hirudinea: Rhynchobdellida) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 1-18 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Morphological and ultrastructural features of the salivary glands and proboscises of Placobdella ornata, Placobdella parasitica, and Desserobdella picta were studied by light and electron microscopy. Chemical composition of the salivary cells was investigated using a variety of histochemical techniques. Placobdella ornata and P. parasitica have compact salivary glands with discrete pairs of anterior and posterior glands, while the salivary cells contain one mucous and three proteinaceous secretions. Salivary glands of D. Picta are diffusely arranged and contain two mucous and two proteinaceous secretions. A cobalt-lysine forward-filling technique revealed that individual salivary cells consist of a roughly spherical soma and an elongated ductule. The majority of the internal space in a salivary soma is densely packed with spherical secretory granules which displace the cytoplasm to the periphery of the cell. Bundles of individual ductules enter the base of the proboscis on opposite sides and extend anteriorly. The ductules, also packed with secretory granules, are surrounded by microtubules associated with agranular endoplasmic reticulum, and merge with deep invaginations of the proboscis cuticle. The secretory granules are released at the end of these invaginations or pores. Pores were found on the tip, along the body, and on the luminal wall of the proboscises in all three species. © 1995 Wiley-Liss, Inc.
    Additional Material: 24 Ill.
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    In vivo strain in the humerus of pigeons (Columba livia) during flight (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 61-75 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Longitudinal and principal strain recordings were made in vivo at three sites (dorsal, anterior, and ventral) on the humeral midshaft of pigeons executing five modes of free flight: Take-off, level flight, landing, vertical ascent, and near-vertical descent. Strains were also recorded while the birds flew carrying weights that were 33%, 50%, or 100% of their body weight. The relative distribution of strain measured at the three surface midshaft sites and across the bone's cortex was found to be similar for all flight modes. Principal strains recorded in the dorsal and ventral humerus indicated considerable torsion produced by aerodynamic loading of the wing surface posterior to the bone. Measured torsional shear strains (maximum: 2,700-4,150 μ ε during level flight) were 1.5 times greater than longitudinal strains. In addition to torsion, the humerus is also subjected to significant dorsoventral bending owing to lift forces acting on the wing during the downstroke. Analysis of the cross-sectional distribution of longitudinal strains at the humeral midshaft cortex shows that the orientation of bending shifts in a regular manner during the downstroke, indicating that the wing generates progressively more thurst (vs. lift) later in the downstroke. This shift is less during take-off and vertical ascent when greater lift is required. Peak principal and longitudinal strains increased by an average of only 50% from landing to vertical ascending flight and take-off (e.g., dorsal humerus: -1,503 to -2,329 μ ε) and did not exceed -2,600 μ epsiv; at any site, even when the birds flew carrying twice their body weight. Strains recorded when birds flew at two times their body weight (100% BW load) were similar in magnitude to those recorded during vertical ascent and take-off and likely represent those developed during maximal performance. Strains developed within the midshaft were maximal in the anterodorsal and posteroventral cortices, not at the dorsal, ventral, and anterior sites at which strain was recorded. Consequently, maximum strains experienced by the bone are probably 20-25% greater than those recorded (ca. 3,200 μ ε), indicating a safety factor of about 3.5 for compressive strain failure. The much higher shear strains, however, indicate a lower safety factor (1.9), in which the bone's torsional strength is its most critical design feature. Finally, the magnitude and distribution of strains developed in the humerus of pigeons are generally similar to those recorded in the humerus of large fruit-eating bats during flight. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052250106
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    Cephalometric correlates of echolocation in the chiroptera: II. Fetal development (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 107-123 
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    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cephalometry was used to detect patterns of cranial growth in fetal bats that were stained differentially for bone and cartilage. Three developmental features distinguish embryos of taxa that echolocate nasally from embryos of taxa that echolocate orally: (1) the basicranium is retained ventral to the cervical axis, (2) the rostrum is retained below the basicranial axis, and (3) the lateral semicircular canals are rotated caudally. Together, the first two actions align the fetal nasal cavity with what will be the long axis of the adult body in flight. The third action aligns the lateral semicircular canals with the horizontal. In contrast, skulls of oral-emitting taxa are constructed such that the oral cavity is aligned with the long axis of the body in flight. The evolution of head posture and skull form in microchiropteran bats has been constrained by the demands of vocalization, i.e., ultrasonic echolocation. Accordingly, the ontogeny of the microchiropteran skull has been canalized along two distinct developmental paths - oral-emitting and nasal-emitting Baupläne. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052250109
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    Masthead (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052250301
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    Effects of caffeine treatment on the fine structure, silica secretion, and shell morphology of the testate amoeba Assulina muscorum (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 251-260 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Assulina muscorum secretes morphologically altered shells when cultured in a medium with 5 mM caffeine. The siliceous scales, normally distributed in a regular overlapping pattern, are disorganized, thicker and wider than normal, and occasionally have incompletely silicified surfaces that appear irregular in profile in transmission electron microscopic ultrathin sections. The shape of the silica deposition vesicles (SDVs) in the cytoplasm is altered and they are less regularly arranged. The swollen appearances of the SDVs, and of nearby Golgi tubules, give additional evidence that caffeine affects the fine structural morphology of membranous secretory organelles and can disrupt their normal depositional activity. In addition to the greater thickness and width of the siliceous scales in caffeine-treated cells, the length and width of the shell are larger compared to controls, but the aspect ratio (length / width) is smaller. The latter is attributed to a larger increase in width relative to the increase in length of the caffeine-reated cells. Since some of the scales are deposited with the long axis laterally on the shell surface, in addition to being greater in width, this raises the interesting question of whether the morphology of the SDVs and the siliceous products influences the size and morphogenesis of the shell. Further research is needed to clarify the interaction of the SDVs with the cytoplasmic cytoskeletal system during shell morphogenesis. © 1995 Wiley-Liss, Inc.
    Additional Material: 12 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052250302
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    Spinal cord central canal of the German shepherd dog: Morphological, histological, and ultrastructural considerations (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995), S. 205-212 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study deals with some macroscopical, microscopical, and ultrastructural aspects of the spinal cord central canal of the German shepherd dog. The caudal end of the spinal cord is constituted by the conus medullaris, which may extend to the first sacral vertebra, the terminal ventricle, and the filum terminale. The latter structure is considered as internum (second to third sacral vertebrae) or externum (fifth caudal vertebra), according to its relation to the dura mater. Occasionally, there is a second anchorage which is close to the level of the sixth caudal vertebra. The central canal is surrounded by a ciliated ependymal epithelium, which differs depending upon the levels. The most caudal part of the filum terminale bears a columnar ciliated ependymal epithelium surrounded by two layers of glia and pia mater, which separate the central canal from the subarachnoid space. Microfil injections show a communication between the cavity and the subarachnoid space, as the plastic is able to pass through the ependymal epithelium. At the level of the terminal ventricle there are real separations of the ependymal epithelium, which seem to connect the lumen of the spinal canal with the subarachnoid space. These structures probably constitute one of the drainage pathways of the cerebrospinal fluid. The diameter of the central canal is related to the age of the animal. However, even in very old animals the spinal cord central canal reaches the tip of the filum terminale and remains patent until death. At the ultrastructural level the ependymal cells present villi, located on cytoplasmic projections, cilia, dense mitochondria, and oval nuclei. © 1995 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052240209
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    Developing embryo and cyclic changes in the uterus of Peripatus (Macroperipatus) acacioi (Onychophora, Peripatidae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995), S. 179-198 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Female reproductive tracts of the viviparous neo-tropical onychophoran Peripatus acacioi have been examined at different times throughout the year, and the altering relationship between the developing embryo and the uterus is described. Depending on her age and time of year, the female may have one or two generations of embryos within her uterus. The uterine wall consists of a thin outer epithelium and basal lamina, three layers of muscles, and a thick basal lamina beneath an inner epithelium lining the uterus lumen. These layers are consistent along the length of the uterus apart from the inner epithelial lining, which varies according to position in the uterus and the developmental stage of embryos contained in the uterus. Early embryos are positioned along the length of the uterus and therefore have space in which to grow. During cleavage and segment formation, each embryo is contained within a fluid-filled embryo cavity that increases in size as the embryo grows. Morulae and blastulae are separated by lengths of empty uterus in which the epithelial lining appears vacuolated. Until the process of segment formation is complete, the embryos are attached to a placenta by a stalk and remain in the same part of the upper region of the uterus. As these embryos grow, the lengths of vacuolated cell-lined uterus between them decrease. Each embryo cavity is surrounded by the epithelial sac, the maternal uterine epithelium, which becomes overlaid by a thin layer of cells, the embryo sac, which is believed to be of embryonic origin. The placenta is a syncytial modification of the epithelial sac located at the ovarian end of each embryo cavity covered by the embryo sac and is analogous to the mammalian noninvasive epitheliochorial placenta. Segment-forming embryos have their heads directed toward the ovary. As the embryo gets longer during segment formation, its posture changes from coiled to flexed. Once segment formation is complete, the embryo loses contact with its stalk, an embryonic cuticle forms, and the embryo turns around so that its head is directed toward the vagina. The embryo escapes from its embryo sac and moves to the lower part of the uterus. In the lower part of the uterus, the straightened fetuses are first unpigmented but subsequently become pigmented as the secondary papillae on the body surface form and an adult-type cuticle forms beneath the embryonic cuticle. While the embryos are contained within their embryo cavities, nutrients are supplied by the placenta. Throughout development the mouth is open and in the mature fetus the gut is lined by peritrophic membrane and material is present in the gut lumen. Trachea have been observed only in fetuses that were ready for birth. Insemination, cyclical changes in the uterine epithelium, and the nature of the cuticle shed at parturition are discussed. © 1995 Wiley-Liss, Inc.
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    Mechanics of the avian propatagium: Flexion-extension mechanism of the avian wing (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 91-105 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The supporting elements of the avian propatagium were examined in intact birds and as isolated components, using static force-length measurements, calculated models, and airflow observations. The propatagial surface supported between Lig. propatagiale (LP) and brachium-antebrachium is equally resistant to distortion over the range of wing extension used in flight. The lengths LP assumes in flight occur across a nearly linear, low-stiffness portion of the force-length curve of its extensible pars elastica. In an artificial airflow, intact wings automatically extend; their degree of extension is roughly correlated with the airflow velocity. Comparisons between geometric models of the wing and the passive force-length properties of LPs suggest that the stress along LP blances the drag forces acting to extend the elbow. The mechanical properties (stiffness) of the LP vary and appear to be tuned for flight-type characteristics, e.g., changes in wing extension during flight and drag. Lig. limitants cubiti and LP combine to limit elbow extension at its maximum, a safety device in flight preventing hyperextension of the elbow and reduction of the propatagium's cambered flight surface. Calculations using muscle and ligament lengths suggest that M. deltoideus, pars propatagialis, via its insertions onto both the propatagial ligaments, controls and coordinates propatagial deployment, leading edge tenseness, and elbow/wing extension across the range of wing extensions used in flight. The propatagial ligaments and M. deltoideus, pars propatagialis, along with skeleto-ligamentous elbow/carpus apparatus, are integral components of the wing's extension control mechanism. © 1995 Wiley-Liss, Inc.
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    Comparative embryology of teleostean fishes. I. Development and staging of the american shad, Alosa sapidissima (Wilson, 1811) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 125-167 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A new approach to devising embryological staging systems allows improved phylogenetic comparisons of developmental patterns. As in traditional approaches, morphological features provide the defining criteria for stages, but unlike traditional normal tables, each stage is defined by no more than one or two morphological criteria that are fundamental developmental features of all teleosts. Additional developmental features that occur concurrently with the defining criteria of a stage are treated as variables potentially uncoupled from the defining criteria for that stage. This system is well suited to detect phylogenetic heterochrony and promises to increase our understanding of conserved vs. labile features in teleostean embryology. In this study, I explain the defining criteria for American shad and then make comparisons with other clupeoids. The development of American shad includes 35 stages extending from fertilization to metamorphosis. Comparisons with other clupeoids indicate that the developmental pattern of shad is representative of the early ontogeny of many clupeoids during the embryonic and yolk sac periods and may be conservative for the group. However, several concurrent features, particularly hatching, formation of neuromasts, and opercular development, vary in developmental timing among clupeoids. Comparisons indicate that shad embryos delay the development of these concurrent features relative to other clupeoids. Modifications of the developmental pattern for different species of clupeoids are heterochronic but their phylogenetic and adaptive significance is unknown. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052250202
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    Fiber-type composition of hindlimb muscles in the turtle, Pseudemys (Trachemys) scripta elegans (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 193-211 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A description is provided of the fiber-type composition of several hindlimb muscles of the adult turtle, Pseudemys (Trachemys) scripta elegans. In addition, cross-section areas of each fiber type and an estimation of the relative (weighted) cross-section area (wCSA) occupied by the different fiber types are also provided. Seven muscles were selected for study, based on their suitability for future neurophysiological analysis as components of the segmental motor system, and on their homologies with muscles in other vertebrates. The test muscles were iliofibularis (ILF), ambiens (AMB), external gastrocnemius (EG), extensor digitorum communis (EDC), flexor digitorum longus (FDL), tibialis anterior (TA), and peroneus anterior (PA). Serial sections of these muscles were stained for myosin adenosine triphosphatase (ATPase), NADH-diaphorase, and alpha-glycerophosphate dehydrogenase (α-GPDH), thereby enabling fiber-type classification on the basis of indirect markers for contraction speed and oxidative (aerobic) vs. glycolytic (anaerobic) metabolism. All muscles contained three fiber types: Slow oxidative (SO; possibly including some non-twitch tonic fibers); fast oxidative glycolytic (FOG); and fast glycolytic (Fg). There were at least 30% FOG and 50% FOG + Fg fibers in the seven muscles, the extreme distributions being the predominantly glycolytic ILF vs. the predominantly oxidative FDL muscle (ILF - 15.5% SO, 35.2% FOG, 49.3% Fg vs. FDL - 49.1% SO, 41.1% FOG, 9.8% Fg). As in other species, the test muscles exhibited varying degrees of regional concentration (compartmentalization) of the different fiber types. This feature was most striking in ILF. Pronounced compartmentalization was also observed in AMB, EG, PA, TA, and EDC, whereas the distribution of fiber types in the highly oxidative FDL was homogeneous. In five of the seven muscles, fiber size was ranked with Fg 〉 FOF 〉 SO. In terms of wCSA, which provides a coarse-grain measure of the different fiber types' potential contribution to whole muscle peak force, all muscles exhibited a higher Fg and lower SO contribution to cross-section area than suggested by their corresponding fiber-type composition. The largest relative increases in wCSA vs. fiber-type composition were in the ILF and AMB muscles. We conclude that the turtle hindlimb provides some interesting possibilities for testing for a division of labor among different muscles during different movements (e.g., sustained vs. ballistic), and for study of the behavior of the different fiber (and motor unit) types under normal and perturbed conditions. The relationships between the present results and previous findings on homologous muscles of the mammalian (cat, rat) and reptilian (lizard) hindlimb are discussed. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052250205
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    Occurrence of ampulla in the ductus deferens of the Indian garden lizard Calotes versicolor daudin (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 261-268 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: During the breeding season, the terminal end of the ductus deferens of Calotes versicolor appears swollen and is comparable to the ampulla of the mammalian ductus deferens. Its anatomy was studied from paraffin sections. It differentiates along its length into five zones. The first has thick smooth muscle and pesudostratified epithelium; the second has luminal trabeculae with an epithelium showing evidence of secretory activity; the third has the epithelial mucosa abutting against the smooth muscle in the form of pocketlike indentations; the fourth has crypts between epithelial folds; and the fifth zone is a sphincter. The anatomy of this ampullary region is indicative of secretory as well as spermatophagous roles. It undergoes seasonal change and appears to be androgen-dependent. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052250303
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    Perinatal changes in avian muscle: Implications from ultrastructure for the development of endothermy (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 357-367 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Endothermic heat production and the capacity to shiver develop soon after hatching in birds, permitting chicks to regulate their body temperature. Physiological studies have not clearly identified the developmental events causing this change in function. Here, we use electron microscopy to examine the development of structures involved in muscle activation, contraction, and metabolism coincident with the development of shivering thermogenesis. A stereological study was used to compare the ultrastructure of chicken iliofibularis before endothermic heat production was present (24 h before hatching) and 120 h later, when the iliofibularis had substantial capacity for shivering. Profound increases were found in the t-tubule system and terminal cisternae, mitochondrial cristae, and lipids. The number of triadic profiles increased 3.8-fold (7.6 ± 1.31/100 μm2 to 28.5 ± 2.90/100 μm2 fiber area). The surface area of cristae per mitochondrial volume doubled (12.0 ± 1.50 pm2/pm3 to 25.7 ± 1.84 μm2/μm3). Lipid droplets were rare in the iliofibularis of embryos about to hatch, but accounted for 4.4% of the muscle fiber volume in day 4 birds. We suggest that these ultrastructural changes more fully activate the iliofibularis, allow it to produce more heat both from calcium pumping and from contraction, and increase its endurance, thus permitting the muscle to be effective in thermogenesis. © 1995 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052250307
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    Masthead (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 226 (1995) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052260101
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    Sensory receptors on the adult labial and maxillary palpi and galea of Cicindela sexguttata (Coleoptera: Cicindelidae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 226 (1995), S. 25-31 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Five types of sensilla are situated on the apical area of the labial and maxillary palpi and galea of Cicidela sexguttata. Large, conical, and peg-like sensilla are in rows on the central region of each palpus. These sensilla have a hollow cuticular peg, with an apical pore and multi-innervation. This central region of palpal sensilla is surrounded by campaniform sensilla that are disc-shaped and small conical peg sensilla. A similar type of conical sensillum as the found in the palpal central region is situated around the periphery of the palpal apex and apex of the galea. This conical peg sensillum is located in a shallow depression and is structurally similar to the other peg sensilla, but it has a mechanoreceptor neuron attached to the cuticular base of the sensillum. A long, single, trichoid sensillum is situated in the center of the galea and is hollow, thick-walled, porous, and multi-innervated. The apices of the palpi and galea have a large number of dermal gland openings that actively secrete a substance during the feeding process of the tiger beetle. © 1995 Wiley-Liss, Inc.
    Additional Material: 15 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052260103
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    Seasonal variation in ovarian histology of the viviparous lizard Sceloporus torquatus torquatus (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 226 (1995), S. 103-119 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Changes in ovarian histology during the reproductive cycle of the viviparous lizard Sceloporus torquatus torquatus are described. In general, the variation in follicular histology observed during the seasonal cycle is similar to that of other lizards. Sceloporus t. torquatus exhibits a cycle in which small, previtellogenic follicles exist in the ovary from December to August. Vitellogenesis occurs between September and November, followed by ovulation from late November to early December. Parturition occurs the following spring. After ovulation, the remaining follicular cells form the corpus luteum and luteolysis did not occur until April-May. Follicular atresia is commonly observed in previtellogenic follicles with polymorphic granulosa, but occurs less frequently in follicles during late vitellogenesis. There are two germinal beds in each ovary. The yolk nucleus is evident in young oocytes as is a vacuolated ooplasma prior to vitellogenesis. Extensive polymorphism is observed in yolk platelets. Mast cells and secretory cells are observed in the thecal layer of the follicular wall as are melanocytes in the ovarian stroma. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052260107
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  • 89
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    Cytological aspects of vascular invasion by the trophoblast of Calomys callosus in hepatic tissues (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 226 (1995), S. 159-171 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Ectoplacental cones isolated from embryos on the seventh day of pregnancy wer transplanted beneath the hepatic capsule of recipeint adult animals to document the morphological patterns of vascular invasion by the trophoblast in the absence of the maternal environment and the influence of its peculiar vasculature. Females, and females and males of Calomys collosus, a cricetid rodent, were used, respectively, as embryo donors and recipient animals. Three to 5 days later, the grafted regions were excised and processed for light and electron microscopy. Invasion of the liver parenchyma by the trophoblast progressed along the vascular beds, associated with gradual phagocytosis of hepatic cells, greatly favoring the morphological characterization of invasive steps exhibited by the trophoblast to access the different kinds of vessels, to trespass the various vascular components and the different levels of the surrounding hepatic parenchyma. It is possible that either in utero during the establishment of embryomaternal circulation in early pregnancy or ex utero under experimental conditions, the trophblast exhibits similar vascular invasion behavior. In view of this, our findings may contribute to a better understanding of trophoblast cell migration to the maternal blood supply as well as the role of the trophoblast in the establishment of the placental circulation during pregnancy. © 1995 Wiley-Liss, Inc.
    Additional Material: 17 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052260205
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    A commentary on the segmental motor system of the turtle: Implications for the study of its cellular mechanisms and interactions (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 225 (1995), S. 213-227 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A commentary is provided on the segmental motor system of the turtle Pseudemys (Trachemys) scripta elegans with an emphasis on neuronal, neuromuscular, and muscular mechanisms that control the development of force under normal, fatiguing, and pathophysiological conditions. For the central neuronal component of the segmental motor system, it has recently been shown that intracellular analysis of the firing properties of motoneurons and interneurons can be undertaken for relatively long periods of time in in vitro slices of the lumbosacral spinal cord of the adult turtle. In other less reduced in vitro preparations, analyses are available on complex motor behaviors generated by the isolated spinal cord. These behaviors of spinal neuronal networks are analogous in key aspects to those generated by the isolated in vivo cord, and by the cord in intact preparations. These results suggest that the neuronal components of the segmental motor system can now be studied from the cellular/molecular level of analysis in in vitro slice preparations to the systems level in conscious, freely moving animals. The in vitro approach can also be used for the analysis of cellular mechanisms in suprasegmental brain structures, which contribute to the control of voluntary movement. For the peripheral neuromuscular component of the segmental motor system, information is now available on muscle fiber types and selected aspects of sensory innervation, and it is feasible to study the mechanical and biochemical properties of motor units. As such, the turtle presents a valuable model for exploring interrelations between the neuronal and mechanical components of the segmental motor system of the generalized tetrapod. A prominent feature of these recent developments is the extent to which they have been deriven by findings that have emphasized an evolutionary conservation of motor-control mechanisms extending from ion channels, at the cellular level, to the control of multijointed movements at the systems level of analysis. © 1995 Wiley-Liss, Inc.
    Additional Material: 1 Tab.
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    URL: http://dx.doi.org/10.1002/jmor.1052250206
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    Diversification of brain morphology in antarctic notothenioid fishes: Basic descriptions and ecological considerations (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 47-83 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The Notothenioidei, a perciform suborder of 120 species, dominates the ichthyofauna of the Southern Ocean around Antarctica. Unlike most teleost groups, notothenioids have undergone a corresponding ecological and phyletic diversification and therefore provide an excellent opportunity to study the divergence of the nervous system in an unusual environment. Our goal is to evaluate notothenioid brain variation in light of this diversification. To provide a baseline morphology, we examine the gross morphology and histology of the brain of Trematomus bernacchii, a generalized member of the family Nototheniidae. We then examine the variation in brain gross anatomy (32 species) and histology (10 species) of other notothenioids. Our sample represents about 27% of the species in this group and includes species from each of the six families, as well as species representing diverse ecologies. For comparison we reference the well-studied brains of two species of temperate perciformes (Perca flavescens and Lepomis humilis). Our results show that, in general, notothenioid brains are more similar to the brains of temperate perciforms than to the unusual brains of cave-dwelling and deep-sea fishes. Interspecific variation in gross brain morphology is comparable to that in Old World cyprinids and is illustrated for 17 species. Variation is especially noteworthy in the ecologically and geographically diverse family Nototheniidae. Measurements indicate that sensory regions (olfactory bulbs, eminentia granularis, and crista cerebellaris) exhibit the most pronounced variation in relative surface area. Association areas, including the corpus cerebelli and the telencephalon, exhibit moderate variation in size, shape, and lobation patterns. Regulatory areas of the brain, including the saccus vasculosus and the subependyma of the third ventricle, are also variable. These regions are best developed in species living in the subfreezing water close to the continent. In some species the expanded ependymal lining forms ventricular sacs, not previously described in any other vertebrate. Three species, including two nototheniids (Eleginops maclovinus and Pleuragramma antarcticum) and the only artedidraconid in our sample, have distinctive brains. The unique brain morphology of Pleuragramma is probably related to a sensory (lateral line) specialization for feeding. Within the Nototheniidae, a phyletic effect on cerebellar morphology is evident in the Coriiceps group and in the Pleuragramminae. Neither phyletic position nor ecological factors (water temperature, position in the water column, dietary habits) alone fully expalin the pattern of notothenioid brain diversification. © 1995 Wiley-Liss, Inc.
    Additional Material: 12 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230107
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    Announcement (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. I 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052230202
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    Skeletal morphogenesis in the urodele skull: I. Postembryonic development in the hemidactyliini (Amphibia: Plethodontidae) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 125-148 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The metamorphic species Eurycea bislineata is presented as a standard for evaluating the relationship between cranial ontogeny and life history in hemidactyliine plethodontid urodeles. Past and present descriptions are combined into a comprehensive summary of postembryonic skull development for this species, and the sequence, sizes of onset, and morphogenetic pathways are documented for all major remodelling events. Developmental series are also compared intraspecifically, between two populations of E. bislineata that differ in metamorphic size, and interspecifically, with species having different larval periods (Hemidactylium scutatum, Gyrinophilus porphyriticus, Pseudotriton ruber, E. wilderae, E. longicauda guttolineata) and with epigean (E. tynerensis, E. nana, and E. neotenes) and subterranean perennibranchiates (Haideotriton wallacei and Typhlomolge rathbuni). Cranial ontogeny is largely conserved in these hemidactyliines despite their disparate life histories. Outside the dichotomy of metamorphic and perennibranchiate development, variation is limited to interspecific differences involving the nasolacrimal duct, the repeated loss of the scleral cartilage, and minor dissociation of several metamorphic and postmetamorphic remodelling events. Some, but not all, of this variation is consistent with evolutionary differences in metamorphic size and age. The comparison of metamorphic and perennibranchiate hemidactyliines reveals a unique cranial ontogeny for urodeles, characterized by the abrupt and synchronous timing of almost all postembryonic remodelling, including the nasal skeleton, and the complete absence of these events in perennibranchiate forms. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230203
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    Cytological and morphological ontogenesis and involution of the thymus in cichlid fishes (Cichlidae, Teleostei) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 175-190 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ontogenesis and involution of thymus in cichlid fishes was studied with the aim of comparing development in the bottom-spawning species Tilapia zillii and T. tholloni, and in the mouth-brooding species Oreochromis auratus, O. niloticus, O. mossambicus, and Sarotherodon galilaeus. For comparison, data are also given on bottom-spawning Cichlasoma spp. from America and mouth-brooding Pseudotropheus auratus and Aulonocara nyassae from Africa. Developmental changes were followed histologically by means of light and electron microscopy of sections, beginning with embryos 24 h after fertilization, up to 14-year-old specimens of O. auratus. In all these fish, the anlagen of the thymus glands begins from the third and fourth gill pouches, and their development shows a high correlation with the pace of general organogenesis, which differs greatly in the bottom-spawning and mouth-brooding cichlids. In juveniles of bottom-spawners of 20-40 mm total length and in mouth-brooders of 40-60 mm total length, three cell types are present in the thymus: thymocytes, with large, dense nuclei; epitheliocytes, with long cell extensions containing bundles of tonofibrils; and reticulocytes, with short, granulated cell extensions. Hassall's corpuscles start to develop in larvae of T. zillii at 20-35 mm total length, and in specimens of 40 mm and more total length the corpuscles are typical, formed by inner and outer rings of epitheliocytes. At 30-45 mm total length, cell debris starts to accumulate in the interior of the corpuscles as an early sign of regression. As involution continues, macrophages accummulate within and around the Hassall's corpuscles. The epitheliocyte rings are eventually completely broken down. Isles of thymocytes persist in tilapias from the age of 1-14 years, but most of the thymus volume is occupied by blood lacunae and pigmented macrophage aggregations. The morphology is similar in the mouth-brooding species Pseudotropheus beginning at 1.5 years of age. © 1995 Wiley-Liss, Inc.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230206
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    Ultrastructure of the ventral sucker of Schistosoma mansoni cercaria (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 215-223 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The ventral sucker of Schistosoma mansoni cercaria is a cupshaped structure that is attached to the ventral surface of the organism by a homogeneous connective tissue that surrounds the acetabular glands. The sucker consists of an extensive complex of circular and longitudinal muscles. The longitudinal muscles extend outwoard in a radial pattern to form the cup of the organ. Intermingled with the muscles are nerve bundles and subtegumental cells (cytons). Dendritic nerve fibers connect to sensory papillae which are found on the surface tegument. Two types of sensory papillae are present: a commonly found unsheathed uniciliated papilla, and a previously unidentified tegumental encapsulated structure. Tegument with spines covers the ventral sucker, although the tegumental encapsulated sensory papilla lacks spines. © 1995 Wiley-Liss, Inc.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230209
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    Announcement (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. I 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052230302
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    Anatomy of neurons crossing the tritocerebral commissures of the cockroach Periplaneta americana (Blattaria) (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 225-242 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The neuronal connections of the tritocerebral commissures of Periplaneta americana were studied in the brain-suboesophageal ganglion complex and the stomatogastric nervous system by means of heavy metal iontophoresis through cut nerve ends followed by silver intensification. The tritocerebral commissure 1 (Tc1) contains mainly the processes of the subpharyngeal nerve (Spn) whose neurons are located in both tritocerebral lobes and in the frontal ganglion. Some neurons of the frontal ganglion project through the Tc1 to the contralateral tritocerebrum. A few fibers in this commissure were observed projecting to the protocerebrum and the suboesophageal ganglion. There are tritocerebral neurons which pass through the Tc1 or the tritocerebral commissure 2 (Tc2) and extend on into the stomatogastric nervous system. One axon of a descending gaint neuron appears in the Tc2. This neuron lies in the tritocerebrum and connects the brain to the contralateral side of the ventral nerve cord. In addition, sensory fibers of the labral nerve (Ln) traverse both commissures to the opposite tritocerebrum. The anatomical and physiological relevance of the identified neuronal pathways is discussed. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052230210
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    Skeletal morphogenesis in the urodele skull: III. Effect of hormone dosage in TH-induced remodeling (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 243-261 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: This study examines the dosage dependency of thyroid hormone (TH)-mediated remodelling in the cranial skeleton of the hemidactyliine plethodontid urodele, Eurycea bislineata. One set of experiments quantifies morphogenetic responses in 21 tissues for four size-age classes of larvae immersed in four different T4 concentrations. A second set varies both the period and concentration of T4 treatment to evaluate the effect of different TH profiles on adult tissue shape. The tissues surveyed in this study exhibit a 100-fold range in TH sensitivity. Those in regressive morphogenesis have tissue-specific sensitivities which correlate with the timing of their remodelling in natural development: bone resorption is more sentitive than cartilage resorption and is initiated earlier in metamorphosis. In contrast, the TH sensitivities of tissues in progressive morphogenesis vary within each tissue type and even within some tissues, and they do not correlate with timing in natural development. Some explanation for this discrepancy is offered by the constant spatial and temporal relationships between nasal cartilage and dermal bone, which suggest that some TH-mediated ossification may additionally require induction by cartilage. Also, the failure of nasolacrimal duct morphogenesis at all but the lowest dosage correlates with the inductdion of integumentary changes that may preclude duct formation. Variable T4 treatments produce no effect upon the adult skull, other than loss of the nasolacrimal duct and/or foramen. These results have two developmental implicatons. First, the dosage dependencies of the nasolacrimal duct, ossification sequences, and cranial remodelling patterns all support a TH profile with exceptionally low levels at larval stages and at least a 100-fold increase at metamorphosis. Second, a small change in the rate of TH activity has the potential to effect a large-scale rearranggement and restructuring of TH-dependent remodelling. The lack of such transformations in metamorphic plethodontids suggests that TH activity is highly conserved in this group. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
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    URL: http://dx.doi.org/10.1002/jmor.1052230303
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    Masthead (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 224 (1995) 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052240101
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    Cephalic morphology of the honey possum, Tarsipes rostratus (Marsupialia: Tarsipedidae); an obligate nectarivore (1995)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 223 (1995), S. 303-323 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The sharply tapering skull of the honey possum is delicately constructed and has only a few, minute teeth; its mandible is reduced to a thin, flexible rod. The mandibular fossa has been diplaced caudally to the caudomedial corner of the squamosal. Head skeletons of the feathertail glider and western pygmy-possum, omnivores that are closely related to the honey possum, bear greater resemblance to the distantly related carnivorous fat-tailed dunnart than to the honey possum.Selected muscles associated with the jaws, hyoid, and tongue of these four mouse-sized (9-22 g) marsupials are described for the first time. The honey possum is characterized by a greatly reduced temporalis that is almost completely hidden by the eye. Its digastric consists of a single belly that inserts onto the caudal margin of the mylohyoid. The lateral pterygoid is relatively long as it extends caudally to insert onto the elongated mandible. The stylohyoid originates high up on the caudal surface of the tympanic bulla; it curves around the caudal and ventral surfaces of the bulla to reach the basihyoid. The insertion of the genioglossus is restricted to the caudal quarter of the tongue. Homologous muscles of the feathertail glider and western pygmy-possum are more similar to those of the fat-tailed dunnart. In addition to the very different musculoskeletal system, the honey possum has an unusual tongue that tapers to a fine point. © 1995 Wiley-Liss, Inc.
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    URL: http://dx.doi.org/10.1002/jmor.1052230307
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