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  • 1995-1999  (43)
  • 1980-1984  (110)
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  • 1996  (43)
  • 1984  (110)
  • seaweed
  • 1
    ISSN: 1573-5176
    Keywords: compression ; LiCl ; Porphyra ; seaweed ; tension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mechanical properties of various differentiated regions of thePorphyra perforata thallus and the effect of LiCl were studied by performing compression and tension tests. Among the various differentiated tissues, the holdfast area was high in its ‘compressive modulus of elasticity’ and ‘tensile modulus of elasticity’, possibly related to its thick matrix. Vegetative non-dividing tissue and vegetative dividing tissue were the most flexible and strong, showing the highest ‘percentage elongation at break’ and ‘tensile strength’. The patch area, which is a transition zone leading to sexually mature tissue, had moderate values of tensile properties. Meanwhile, sexually differentiated male and female tissues had the highest ‘compressive modulus of elasticity’ and lowest tensile properties. Thes tisues tended to crumble easily. Treatments in LiCl, as used for DNA extraction, resulted in a decrease in both ‘compressive modulus of elasticity’ (87%) and ‘tensile modulus of elasticity’ (54%). After treatment of tissue for chromosome staining in a method using LiCl, there was a marked decrease in ‘tensile modulus of elasticity’ (49%), while the ‘compressive modulus of elasticity’ remained unchanged. Such mechanical changes verify the softening effect of LiCl on the seaweedP. perforata tissue.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 321 (1996), S. 77-81 
    ISSN: 1573-5117
    Keywords: seaweed ; Durvillaea antarctica ; sulfated polysaccharides ; fucose ; anticoagulant activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Durvillaea antarctica was extracted with hot water and a sulfate containing polymer was obtained after separation of laminaran and alginic acid with cetrimide and 2% calcium chloride solutions, respectively. Gel electrophoresis showed that the acidic polysaccharide (23.5% sulfate, 4.5% uronic acid) was not homogeneous. It was fractionated by anion-exchange chromatography on DEAE Sephadex with increasing concentrations of KC1 solutions affording seven fractions. All the fractions showed blood-anticoagulant activity. The fraction eluted with 1.2 M KC1 (34.85% sulfate, 1.4% uronic acid) showed the highest thrombin time. The plaque inhibition and the transcription assays of the whole acidic polysaccharide and the partial hydrolysis product against the infectious pancreatic necrosis virus showed a slight antiviral effect. The fucose-containing polymer from D. antarcica is a mixture of sulfated polysaccharides with a different content of sulfate groups and uronic acids. A correlation was observed between the anticoagulant effect uronic acids content.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 371-379 
    ISSN: 1573-5117
    Keywords: Chile ; cultivation ; exploitation ; Gracilaria ; landings ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the last fourteen years the production of seaweeds in Chile has ranged from 74 000 to 229 000 wet metric tons per year and has included about twenty species belonging to Phaeophyta and Rhodophyta. The only source of this production has been the exploitation of natural beds, except for Gracilaria, which is the only case of commercial cultivation and contributes significant quantities to total production. Initially most of the raw material was exported but currently important quantities of Gracilaria and several carrageenophytes are being processed by local industry. Changes in production of the main resources are analyzed with consideration of potential demand, level of knowledge about natural beds, and the situation of total Gracilaria farming, in order to attempt predictions for the supply. Current possibilities of applying new technologies to cultivate other economically important Chilean seaweeds are also analyzed and discussed.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 387-392 
    ISSN: 1573-5117
    Keywords: fusion ; histocompatibility ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fully developed thalli of Chondrus crispus, Gracilaria chilensis, Gymnogongrus furcellatus and Mazzaella laminarioides were used to assess tissue compatibility. The effect of thallus polarity on grafting and regeneration was also evaluated. Fusion did occur between fragments of the same life history phase in C. crispus, G. chilensis, G. furcellatus and M. laminarioides. Fusion between sporophytic and gametophytic tissue occurred in C. crispus, G. chilensis and M. laminarioides. Intergeneric fusion was observed between C. crispus and M. laminarioides, but not between G. chilensis and G. furcellatus. Outer cell wall, cortex and medulla were continuous at the contact face in compatible combinations. Medullary cells in the attached fragments were thinner and longer than normal cells, forming an interwoven scar plate. Thallus polarity did not modify fusion and regeneration.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 429-436 
    ISSN: 1573-5117
    Keywords: codon usage ; Gracilaria verrucosa ; intron ; nuclear protein-coding genes ; poly(A) signal ; promoter ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Single-copy nuclear genes encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase (GapC), triosephosphate isomerase (TPI1) and polyubiquitin (UBI6R), plastid-localized GAPDH (GapA), and mitochondrial aconitase (m-ACN), together with their corresponding cDNAs, have been cloned or PCR-amplified from Gracilaria verrucosa, sequenced, and characterized by genomic Southern hybridization. Three spliceosomal introns were found, one each near the 5′ end of GapA, GapC, and m-ACN. Codon usage is biased, with third-position A's underrepresented. Nucleotide compositions of the genes are balanced except in TPI1. Upstream promoter structure, downstream poly(A) processing signals, intron splice junctions, and one translation initiation site were analyzed. Poly(A) processing features of these red algal genes resemble those of green plants more than those of animals.
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  • 6
    ISSN: 1573-5117
    Keywords: antimicrobial activity ; Caribbean ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Antibacterial activity of lipid extracts from three species of Caribbean marine algae, Spyridia filamentosa, S. hypnoides and Wrangelia bicuspidata was evaluated monthly for one year. Activities were tested for whole plant extracts and TLC-separable zones. Whole plant extracts demonstrated monthly variability in activity with respect to both habitat and life history phase in addition to periods of similarity. No consistency was seen regarding activity against different microorganisms. TLC analyses of the extracts led to the identification of twenty-seven chromatographically distinct regions (TLC zones) each from both S. filamentosa and S. hypnoides and twenty-five from W. bicuspidata, which demonstrated antimicrobial activity. Between these species, twenty-one active TLC zones appeared to be shared based on their similar chromatographic characteristics. Individual TLC zones also demonstrated variable activity throughout the sampling period with respect to habitat and life history phase as well as periods of similarity. Algal antibiosis in these species is recognized as being highly complex, involving numerous chemical compounds, each of which is highly variable in terms of its presence and/or probable concentration.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 491-495 
    ISSN: 1573-5117
    Keywords: acidic polysaccharide ; Leptosomia simplex ; Palmaria decipiens ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Palmaria decipiens, one of the most abundant red seaweeds of the chilean Antarctic, was collected in King George Island. The hot water extract (26% yield) showed by acid hydrolysis to contain xylose, galactose and traces of glucose. Fractionation with cetrimide gave a soluble neutral xylan and an insoluble fraction. The insoluble fraction afforded an acidic polysaccharide that contained 4.8% of uronic acids, 2.8% of sulfate and 18.9% of protein. Polyacrylamide gel electrophoresis showed that it was homogeneous. The GLC and HPLC analysis of the total acidic hydrolysis products showed that the acidic polysaccharide was composed of the neutral sugars galactose and xylose in the molar ratio 8.2:1.0 and of galacturonic and glucuronic acid in the ratio 1.5:1.0. The second-derivative FT-IR spectrum showed the characteristic amide I, II and III bands of proteins. Alkaline cleavage with 0.1 M NaOH indicated the presence of a glycoprotein with O-glycosidic linkage. Results found in this work suggest that the acidic polysaccharide extracted from Palmaria decipiens is an acidic xylogalactan-protein complex.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 401-406 
    ISSN: 1573-5117
    Keywords: CO22 ; HCO inf3 sup− ; light ; Lomentaria ; Palmaria ; seaweed ; sunflecks
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Palmaria palmata, which is able to use HCO inf3 sup− as a carbon source for photosynthesis, and Lomentaria articulata, which is dependent on diffusive uptake of dissolved CO2, were grown under constant light and light with sunflecks designed to model wave-induced fluctuations of near-shore underwater light. Both species exhibited significantly increased stable carbon isotope discrimination (more negative values of δ13C relative to PDB) when grown with sunflecks. More negative δ13C values were associated with decreased growth rate of P. palmata but not of L. articulata. The contrasting effects of sunflecks on the carbon-use characteristics of the two species are discussed in terms of the energetic cost of HCO inf3 sup− use and the susceptibility of CO2 diffusion-dependent species to photoinhibition.
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  • 9
    ISSN: 1573-5117
    Keywords: diatoxanthin ; Gracilaria ; photoinhibition ; phytoplankton ; seaweed ; xanthophyll-cycle ; zeaxanthin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microalgae and macrophytes adapt their pigment content to the environment because excessive light could limit their photosynthetic rate by inducing photoinhibition. Carotenoids participate in the photoadaptative response especially through the operation of xanthophyll cycles (violaxanthin-zeaxanthin or diadinoxanthin-diatoxanthin). An increasing gradient of diatoxanthin in phytoplankton chromophytes is found from the inshore to the offshore waters, less turbid in relation to the different light penetration in seawater. In addition, a nyctemeral cycle is noted, with a suppression of diatoxanthin at night and its accumulation with the increase of the light. Similarly the vertical distribution, on the French Brittany coasts, of several Gracilaria and Gracilariopsis species corresponds to increasing zeaxanthin amounts in the seaweeds living at the upper zones, which are more resistant to photoinhibition as shown by fluorescence and oxygen evolution analysis. An operating xanthophyll cycle should be regarded as a regulatory mechanism involved in stress response for the dissipation of excessive excitation energy through deepoxidated xanthophylls such as zeaxanthin or diatoxanthin.
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  • 10
    ISSN: 1573-5117
    Keywords: agar ; agarophyte ; Gelidium ; phosphate ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phosphate concentration of the growth medium was found to affect the growth rate and agar yield of a clone of Gelidium robustum grown in the laboratory. To study differences in growth we used phosphate concentrations from 0 to 200 µM. To determine the effect of phosphate on agar yield and its properties we used concentrations from 0 to 20 µM. Growth rates generally increased with increasing phosphate concentration, with the highest growth rate (21% d−1) obtained at 150 µM. Agar yield as percentage of fresh weight was highest (10%) in the algae grown with low phosphate concentrations, but agar yield as percentage of dry weight was highest(43%) at 20 µM of phosphate. Gel strength increased with phosphate concentration with a maximum of 160 g m−2 for 0.75% gels for the cultures at 20 µM. Melting and gelling temperatures of the gels were also affected by phosphate concentration of the growth medium. Starch yield was highest in algae grown in low phosphate concentrations.
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  • 11
    ISSN: 1573-5117
    Keywords: airborne ; algorithm ; CASI ; intertidal ; imaging spectrometry ; seaweed ; Spectron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this paper is to test the ability of imaging radiometers to describe the principal seaweed and seagrass beds along the coast of Brittany (France). In this work we used CASI, an instrument with programmable narrow bands. On the ground, regions of homogeneous vegetation cover were mapped using differential GPS positioning. Ground spectra were recorded with a field spectroradiometer (Spectron SE 590), for substratum and different species. Their analysis shows variations in relation to pigment characteristics, vegetation structure and environmental conditions. An algorithm sequence based on field work and according to the technical sensor characteristics, was developed to discriminate the dominant species. The classified CASI image was compared with ground data. The main results are the following P ] (a) the visible wavelengths allow good discrimination between green, red and brown algae. (b) the infrared wavelengths allow separation of two main types of brown species, seagrasses and the identification of floating seaweed.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 191-197 
    ISSN: 1573-5117
    Keywords: epiphytes ; niche partitioning ; rocky shore ; seaweed ; temporal and spatial variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seasonal variation in a Fucus serratus assemblage was studied on the Isle of Man, UK over a 13-month period. Algal cover varied greatly, related to shedding reproductive fronds and winter storms. Peak reproduction was recorded in the autumn. There was little change in density of plants, the population was dominated by a bank of germlings (〈20 cm) and an adult mode between 50–70 cm. An indistinct third component of recruiting juveniles was present when gaps in the canopy formed. Seasonal variation in the algae was reflected in the population dynamics of the mobile epiphytes such as Littorina mariae and Lacuna pallidula, which exhibited annual life histories. Sessile species (such as bryozoans) utilized the algae year round, dominating the perennial stipes and lower fronds, which were not shed. Other species utilized the algae in a transient fashion: some feeding on the algae (e.g. Idotea), or other epiphytic animals (e.g. Nucella). Fucus serratus, therefore, provides a variety of spatially and temporally variable niches that explain the diversity of epiphytic animal species that exploit this alga.
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  • 13
    ISSN: 1573-5117
    Keywords: herbivory ; intra-population variability ; kelp ; Lessonia ; phenols ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Micropopulation differences in phenol content between intertidal and subtidal individuals of the kelp Lessonia nigrescens were found. Subtidal plants showed: (1) significantly higher phenol content than intertidal individuals, in vegetative and reproductive tissues, (2) intra-plant differences, with higher content in apical frond tissues, (3) higher resistance to consumption by herbivorous fishes. The microscopic progeny of subtidal plants showed the same trend as adult plants: (1) haploid spores from subtidal plants had higher phenol content than spores from intertidal individuals, and (2) the microscopic sporophytes derived from subtidal spores and gametophytes were less consumed by herbivorous snails (Tegula tridentata) than those derived from intertidal plant propagules. No increase in phenol content was detected after mechanical injury to experimental fronds, or after transplantation to the subtidal environment. In addition to the absence of inducible responses, the different phenol content between intertidal and subtidal individuals, in adult diploid plants and also in the haploid progeny, suggests that both environments differ someway enough to fix the mentioned features on the plants of Lessonia nigrescens. It is likely that the differences in herbivory between the two distributional extremes contributed to the observed pattern.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 326-327 (1996), S. 29-34 
    ISSN: 1573-5117
    Keywords: Codium ; introduced species ; rDNA ; ITS ; RFLPs ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Analysis of DNA can help to distinguish those morphological characters indicative of species difference from those representing retained traits or parallel evolution. This can be of great value in detecting recent invaders. The choice of which DNA characters to examine not only dictates the methodology to be used but must also be appropriate for the detection level sought. Restriction endonuclease fragment comparisons of plastid DNA have been used to assess Codium species; the results show C. fragile subsp. tomentosoides from east and west coast North America to be identical while sympatric endemic Codium species each display their own unique set of fragments. For species of other algae, plastid DNA fragment patterns are not necessarily identical across a morphological species, e.g. Pandorina morum. Such repetitive element probes as M13 and the use of RAPDs are more appropriate for analysis of populations within species. DNA base sequence comparisons of nuclear rDNA genes often yield too few variant bases between closely related species for reliable identifications. Analysis of the more variable Internal Transcribed Spacer (ITS) region, lying between the small and large ribosomal subunit genes in nuclear DNA, yields more extensive base pair variation between species and relatively little within species; it may be an alternative choice for endonuclease restriction fragment analysis or for sequencing.
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  • 15
    ISSN: 1573-5117
    Keywords: biological interaction ; depth distribution ; Laminaria ; light ; seaweed ; Streblonema ; upper limit ; UV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The kelp Laminaria saccharina dominates soft bottoms in 4–10 m depth in Kiel Bay. Experimental sporophytes transplanted to 2 and 5 m depth showed the typical annual growth pattern of Laminaria species. Surprisingly, 2 m plants died after the first resting phase, whereas 5 m plants survived and showed outgrowth of a new blade generation. Thalli at both depths were infected with the brown algal endophyte Streblonema aecidioides, with host deformations being significantly stronger in 2 m plants. Growth rates of infected sporophytes were reduced. Exclusion of UV light in 2 m depth resulted in less infected thalli. Discs excised from L. saccharina and cultivated in different photon fluence rates from 10–600 µmol m−2 s−1 did not differ in growth rate, photosynthesis or dark respiration. Hence, an exclusion of L. saccharina from shallow depths caused by high light cannot be concluded. We suggest the biological interaction with the endophyte S. aecidioides, amplified by UV light, to be most important for the exclusion of L. saccharina from shallow depths in the western Baltic.
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  • 16
    ISSN: 1573-5117
    Keywords: Ascophyllum nodosum ; Nova Scotia ; Canada ; population structure ; recruitment ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the structure of the Ascophyllum nodosum population at Pubnico, southwest Nova Scotia, Canada, at an experimental site subjected to mechanical harvest and at two control sites never subjected to mechanical harvest were monitored from 1991 to 1994. A bimodal population structure measured in terms of plant length was characteristic of all these sites before the experiment. The population structure of the experimental site became unimodal immediately after experimental harvest by machine and remained unimodal for the subsequent two years. However, a bimodal population structure began to appear in the third year. A bimodal population structure remained evident at the control site with bedrock as the substratum but was less evident at the other control site where the substratum is made up of boulders and cobbles. Movement of loose rocks with rockweeds still attached may have contributed to the less distinct modal structure of this control site. Other sites with different harvesting histories monitored in the summer of 1992 showed some interesting patterns. A unimodal population structure was evident in Argyle Sound and Pubnico Point South and at Charlesville, which had been harvested one and two years before, respectively. A bimodal population structure was more evident at Frenchman's Point, which had been harvested three years prior. The rate of change from a unimodal to a bimodal population structure may depend on the intensity of harvest. Extensive canopy removal in intensively harvested areas may be conducive to an influx of recruits and to regeneration from the holdfast. Hence, plant length modal structure may be a useful measure of the relative state of recovery of a harvested population.
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  • 17
    ISSN: 1573-5117
    Keywords: competition ; seaweed ; theoretical models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Lotka-Volterra competition model was used to represent the interaction between Laurencia obtusa and Hypnea spinella. A new model that considers effects of competition on algal carrying capacity is suggested. To test the models, data from field experiments conducted in an intertidal region at Cabo Frio Island, Rio de Janeiro, Brazil, were used. Both models showed that Hypnea was a stronger competitor than Laurencia. The model of interaction through the carrying capacity showed a stable coexistence between the algal populations and better represented the experimental data.
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  • 18
    ISSN: 1573-5117
    Keywords: ammonium ; competition ; eutrophication ; Gracilaria ; seaweed ; Ulva
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In spring (August) 1993 a bloom of Ulva lactuca appeared for the first time in Saldanha Bay, South Africa, and persisted through summer. Ulva wash-ups contaminated the beach and part of the commercial Gracilaria beach-cast had to be discarded. The biomass and distribution of Gracilaria and Ulva are described in relation to the seasonal water chemistry of the bay. Gracilaria survives in deeper water in summer by the pulsing of nutrients on an approximately 6-day cycle of movement of the thermocline that separates nutrient-rich bottom water from warm oligotrophic surface water. Ammonium-rich fish-factory discharge into this surface layer in a sector of the bay provided localised conditions for Ulva to out-compete Gracilaria at depths of 2–5m, demonstrating the powerful disruptive effect of eutrophication in this strongly stratified system.
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  • 19
    Electronic Resource
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    Springer
    Hydrobiologia 326-327 (1996), S. 305-310 
    ISSN: 1573-5117
    Keywords: Clyde ; environmental impact ; marine pollution ; metals ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this work is to describe changes in heavy metal concentrations in Ascophyllum nodosum from 1964 to 1994. Samples were collected from three sites in the Firth of Clyde and analysed for zinc, manganese, iron, copper, lead and nickel. The results were analysed using the multivariate technique Principal Components Analysis (PCA). At the Wemyss Bay site there was a trend towards increasing lead and nickel over the study period, which could not be accounted for by local industrial activity. At the Hunterston site, two groups were well separated by the PCA ordination, based on manganese and zinc concentrations, which corresponded to land reclamation activities in the area. The separation of samples at the Ardneil Bay site correlated well with copper concentration and this corresponded to the termination of industrial effluent with heavy copper loadings. Other changes in industrial effluent were also reflected in the Hunterston and Ardneil Bay site ordinations. The PCA technique highlighted the interplay between metals. The work demonstrated the potential for using multivariate analysis of seaweed metal concentrations in monitoring a posteriori the environmental impact of industrial change.
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  • 20
    ISSN: 1573-5117
    Keywords: Gracilaria ; morphotypes ; RAPD analysis ; RFLP analysis ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The extreme phenotypic variability recognized among the species of Gracilaria has highlighted the need for the application of refined methods to help solve taxa identifications. In Chile, there still exists uncertainty about the exact number of Gracilaria species. Our investigations are centered on DNA analyses of morphotypes collected from different geographical locations, namely Lenga and Isla Santa María, Region VIII (36°00′ S to 38°00′ S), and Maullín, Region X (39°30′ S to 43°40′ S). These two regions of Chile are considered as areas of confluence of G. chilensis, G. verrucosa, and a species of Gracilariopsis. In this study four morphotypes, from a natural bed located in Maullín, were analyzed for RFLP of plastid DNA and the results compared with data of four morphotypes from a bed in Lenga. The DNA banding patterns from each enzyme digest were identical irrespective of morphotypes and/or locations. In an attempt to unravel the nature of the morphological differences found among Lenga and Maullín morphotypes, RAPD analyses of nuclear DNA were also performed; however, no polymorphism has been found yet. Therefore, the data of this study, as well as concurrent data from preliminary interfertility tests, suggest that all morphotypes belong to a single taxon, Gracilaria chilensis.
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  • 21
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    Hydrobiologia 326-327 (1996), S. 353-354 
    ISSN: 1573-5117
    Keywords: aquaculture ; biomass ; Gracilaria ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes experiments to grow a local and still unidentified species of Gracilaria in shrimp hatcheries in Ecuador. The experiments used outdoor tanks of 1 and 18 m3 capacity, with continuous aeration and water renewal every two and five days, respectively. The sea water (salinity 34 ppt) was enriched with Guillard's f/2 medium; light and temperature were monitored but not controlled. One kg of fresh seaweed, inoculated into each tank, produced a biomass of ca. 3 kg in a period of 35 days in the 1 m3 tank and 18 kg in 43 days in the 18 m3 tank. We therefore believe that it is technically feasible to use the large infrastructure of existing shrimp hatcheries in Ecuador to produce Gracilaria.
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  • 22
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    Hydrobiologia 326-327 (1996), S. 367-370 
    ISSN: 1573-5117
    Keywords: culture ; Gelidium rex ; re-attachment ; seaweed ; spores
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Research on the culture of Gelidium rex was approached from two points of view, growth of thalli from spores and growth from re-attachment. Mollusk shells, which are very easy to handle, were used in both systems. The results achieved by these methods showed that reattachment is the technique that obtains larger plants in a shorter time. This is the first stage in developing culture and cultivation techniques aimed at commercial exploitation of this species in Chile.
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  • 23
    ISSN: 1573-5117
    Keywords: agar ; Brazil ; Gelidiales ; Pterocladia ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pterocladia capillacea forms dense intertidal belts in southeastern Brazil, on moderately exposed rocky coasts. The studied population extends along a gradient of water exposure, where slightly different morphotypes can be recognized. Specimens were collected monthly from 3 points along the exposure gradient of its distribution (lower, medium and higher exposure), and analyzed for agar, sulfate and 3,6 anhydrogalactose content. Agar varied from 5–32% of dried seaweed with lower yields in the winter, and higher yields in late spring/early summer. Specimens from the surf side of the distribution had a consistently higher agar content throughout the year. Sulfate varied from 1–5%, and 3,6 AG from 27–48% of dried agar, without a clear variation among the sites.
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  • 24
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    Hydrobiologia 326-327 (1996), S. 511-514 
    ISSN: 1573-5117
    Keywords: Delesseria ; seaweed ; sterol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On Normandy coasts, the red alga Delesseria sanguinea perennates by its stipe; fronds grow in January and disappear in June. Seasonal variations in sterol composition in relation to the biology of D. sanguinea are reported. Sterols in cellular membranes are free or conjugated by esterification with fatty acids, heterosides or lipid complexes like phospholipids. Both kinds of sterols were analyzed by GC-MS. The major sterol (80%) found in fronds was cholesterol whereas in stipes, cholesterol was also the major sterol in spring, but in September, an important reduction in cholesterol yield was noted with proportional increase in sitosterol content. It appears that cholesterol is synthesized in fronds in spring, then transferred to the stipe, which loses an important amount of cholesterol with loss of the blades.
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  • 25
    ISSN: 1573-5117
    Keywords: alkali-soluble alginate ; block structure ; boiling water-soluble alginate ; brown alga ; Kjellmaniella crassifolia ; M:G ratio ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Kjellmaniella crassifolia, the edible macro-brown alga in Japan contained nearly 27% of alginates of which nearly 7% was extractable from the fronds with boiling water for 6 h and the residual alginates in the frond were almost exhaustively extracted with a dilute alkali at 60 °C for 6 h. The alginates dissolved in all these extracts with both boiling water and dilute alkali were purified by fractionation with MgCl2 and alcohol. The content of MM blocks in the boiling water-soluble alginate sample increased remarkably during heating for 6 h while that of GG blocks from the same sample decreased. In contrast, MM blocks in the alkali-soluble alginate sample decreased during 6 h heating while GG blocks continued to increase. Since the amounts of MG blocks showed slight fluctation, the M:G ratio of alginates extracted with boiling water increased towards the end of extraction whereas the reverse is true for the alkali-soluble alginates.
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  • 26
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    Hydrobiologia 326-327 (1996), S. 35-57 
    ISSN: 1573-5117
    Keywords: carrageenan ; Eucheuma ; filtered carrageenan ; Kappaphycus ; natural grade carrageenan ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carrageenan has annual sales of over US$ 200 million, about 15% of the world use of food hydrocolloids. The market for carrageenan has grown exponentially at 5% per year for at least 25 years: 5 500 metric tons in 1970, and over 20 000 metric tons expected in 1995. The industry has become dominated by very large, multi-product companies with carrageenan factories in Europe and the US, but factories are now springing up in the Philippines and Chile, where red seaweeds grow in abundance. About 80 000 tons of dry red seaweeds are needed to produce 20 000 tons of carrageenan. About 40 000 tons comes from the Philippines, 15 000 tons from Indonesia, 15 000 tons from Chile, and 10 000 tons from elsewhere. Carrageenan growth depends on food fads like the McLean hamburger and food winners like processed pork and turkey. Carrageenan is a regulated food additive, and current health concerns focus on the minimum safe molecular weight for carrageenan when eaten. The most innovative development in carrageenans in recent years has been the introduction of a food grade version of lower cost natural grade carrageenan. Its acceptance, however, has been hampered by strong resistance from conventional carrageenan producers.
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  • 27
    ISSN: 1573-5117
    Keywords: biofilter ; floating enclosed systems ; integrated aquaculture ; mussels ; salmon ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A theoretical model linking the production of salmon, mussels and seaweed in floating, enclosed units is based on field data, laboratory tests and literature data. First step is the production of 300 metric tons of salmon in 12 production units of 500 m3 each and a total water flow of 60 m3 min−1. Using a standard high energy dry feed with a feed conversion factor of 1, a production of 300 metric tons of salmon is calculated to produce 15 metric tons of nitrogen (N) and 2.4 metric tons of phosphate (P), of which 13 metric tons of N and 0.7 metric tons of P are dissolved. Outlet water is transferred from the salmon units to 12 closed units (500 m3 each) with blue mussels. A standing stock of 112.5 metric tons of mussels (WW) is necessary to filtrate 60 m3 min−1. If all particles are filtrated through the mussels, 25% of the nitrogen is accumulated as increased biomass. 25–30% is released as faeces and 45–50% as dissolved matter. Particles are removed by a sedimentation trap. Outlet water from the 12 mussel filter units, containing 13.9 metric tons of dissolved N (0.9 metric tons from the mussels), is transferred to closed units (1000 m3) with seaweeds. Concentration of N is calculated to 0.44 g N m−3 and hydraulic retention time in the seaweed production unit is 4 hours. With a DW of 20% (4% N of DW) and an estimated growth rate of 10% d−1 during the production period, a standing stock of 45 metric tons (FW) of seaweed is theoretically needed to bind up all dissolved N from the salmon and mussel production.
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  • 28
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    Hydrobiologia 326-327 (1996), S. 145-148 
    ISSN: 1573-5117
    Keywords: algal taxonomy ; Brazil ; Gracilaria ; Gracilariopsis ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Most of the agar production in the world now comes from terete species of Gracilariaceae, a notoriously difficult group with regard to species circumscription. We have studied different populations of such algae from Brazil and from abroad in order to ascertain their identity and relationship. We based our taxonomic concepts on traditional morphological markers and attempted crosses between putatively interspecific and geographically isolated intraspecific populations. Data from the crosses helped in the recognition of genera and species with convergent morphologies and between disjunct populations. Our results show that configuration of male reproductive structures and some features of cystocarp anatomy are reliable taxonomic characters whereas some morphological features, such as gross thallus morphology and branching pattern, are not.
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  • 29
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    Hydrobiologia 326-327 (1996), S. 173-178 
    ISSN: 1573-5117
    Keywords: diversity ; endemism ; global ; Phaeophyta ; seaweed ; upwelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Brown algal species diversity is compared in 100 km sections of the coastlines of four warm temperate regions: southern Australia, California, southwestern Africa, north-central Chile. The highest diversity (over 140 species per section) is found in southern Australia. California has a reasonable diversity (around 70 species per section), and both southern Australia and California have high regional endemism. Sections of north-central Chile and southwestern Africa have similar patterns, with low diversity (〈 30 species per 100 km section), low endemism, few or no fucoids, and up to 25% of the brown algal flora are environmentally tolerant species of Scytosiphonales. Species turnover between contiguous sections of coast is generally related to relative change in temperature regime. Thus the high diversity of southern Australia is due to high species diversity within the 100 km sections, with little turnover, except for a rapid reduction in eastern Victoria likely to be related to lack of rocky substatum. It is hypothesized that low diversity and endemism in Chile and southwestern Africa can be explained by the occurrence of major environmental perturbations (upwelling and El Nino effects) in these regions, producing variable inter-annual temperature conditions that select out tolerant species from the local floras.
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  • 30
    ISSN: 1573-5117
    Keywords: Fucus serratus ; Himanthalia elongata ; iteroparous ; reproductive allocation ; reproductive effort ; seaweed ; semelparous
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Reproductive allocation, the proportion of total dry weight allocated to receptacle tissue and reproductive effort, the proportion of reproductive carbon requirement contributed by receptacle photosynthesis, were measured in two fucoid algal species Fucus serratus and Himanthalia elongata at sites in NE Scotland. Reproductive development takes over ten months in H. elongata, a semelparous (single reproductive event) species, and reproductive allocation at receptacle maturity is over 98%. Following gamete release, the whole thallus dies. In contrast, reproductive development in F. serratus takes four months. Fucus serratus is iteroparous (capable of multiple reproductive events), reproductive allocation is 38.6% for the first reproductive event and 50.5% for the following year's event. In Fucus serratus, the receptacles appear to make a major contribution to their own carbon requirements after the first month of reproductive development. The receptacles of Himanthalia elongata contribute only a small proportion of the receptacle carbon requirements in the first four months of reproductive development, after which the contribution made through receptacle photosynthesis increases.
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  • 31
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    Hydrobiologia 326-327 (1996), S. 213-215 
    ISSN: 1573-5117
    Keywords: Argentina ; seaweed ; southwest Atlantic ; Undaria pinnatifida ; wakame
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In December 1992, some sporophytes of the Asian kelp Undaria pinnatiftda were found growing subtidally at 6 m depth below ‘A. Storni’ Port, Puerto Madryn, Argentina. During the winter of 1994, the species expanded significantly from its original location. Sporelings appear in early autumn and attain their maximum size (1.65 ± 0.10 m) during winter and early spring, when most of them become fertile. The fronds are lost in summer, with only some holdfasts and sporophylls surviving, and these disappear by the end of summer. The occurrence of U. pinnatida in Golfo Nuevo is reportedly due to an accidental introduction by cargo ships or fishing vessels arriving from Asian ports.
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  • 32
    ISSN: 1573-5117
    Keywords: aliens ; Atlantic ; Laminariales ; seaweed ; Undaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Since its first introduction into the Atlantic for farming purposes in 1983, the brown alga Undaria pinnatifida has been found at several sites along the European coasts. The present study deals with an ecological experiment that was performed at the island of Ushant (Brittany, France) in order to check the ability of Undaria to recruit to a rocky substratum in situ. On the one hand, the authors were unsuccessful in getting Undaria to colonize a previously denuded area of shore, which was subsequently colonized by other native species, a result that may speak for a competition between Undaria and the local kelps. On the other hand, evidence is presented that Undaria pinnatifida has become a member of the Atlantic flora for good. The sporophytes can be found growing from low water mark of neap tide (+1.5 m) down to at least −15 m below lower water mark of spring tide. Its propagation is thought to occur in at least two ways: either step by step from cultivation sites along the coasts, or from the hulls of ships sailing from harbour to harbour. The preference of Undaria for settlement is on artificial structures.
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  • 33
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    Hydrobiologia 326-327 (1996), S. 223-228 
    ISSN: 1573-5117
    Keywords: fecundity ; Gelidium sesquipedale ; Portugal ; reproduction ; seaweed ; size ; spore recruitment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gelidium sesquipedale fecundity was quantified by counting tetrasporangial sori and cystocarps per meter squared and by estimating the number of spores contained inside them. These were obtained by regression on a size metric of reproductive structures. Tetrasporangial sori length and cystocarp thickness were the best estimators of spore number. To assess spore recruitment, 12 pottery tiles were fixed to the bottom, and the appearance of small fronds was monitored. No clear seasonal pattern of reproduction was found. Tetraspore production peaked in March 1990 with 10.4 × 106 spores m−2, whereas the carpospore peak was lower, 4.9 × 105 spores m−2 in July 1989. Recruitment followed tetraspore peaks. The probability of a G. sesquipedale tetraspore making the transition to a recruit was 4.7 × 10−5. Frond length was significantly related to tetrasporangial sori number, while cystocarp number was only related to frond branching order. Minimum size for reproduction was 6.9 cm for gametophytes and 5.4 cm for tetrasporophytes; very rarely were cystocarpic fronds smaller than 9 cm, while tetrasporic fronds were often longer than 15 cm. Cystocarpic fronds were significantly shorter and had more branches than tetrasporic fronds.
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  • 34
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    Hydrobiologia 326-327 (1996), S. 253-258 
    ISSN: 1573-5117
    Keywords: Gigartina skottsbergii ; management ; seasonal variation ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study reports the variations in biomass and reproductive phenology in a natural bed of Gigartina skottsbergii over a period of a year and correlates these variations with the abiotic factors solar radiation, number of daylight hours, water temperature and salinity. The results obtained show an annual production cycle with maximum biomass values in spring-summer, correlated with high solar radiation and to a lesser extent with salinity. Sexual reproduction was maximum in autumn-winter, correlated with low temperature and short-day conditions. The population showed a large gametophytic dominance according to size and biomass, which suggests that there is not ecological equivalence between the life history phases of the species. Finally, based on the results, a restriction of harvesting to spring-summer is suggested.
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  • 35
    ISSN: 1573-5117
    Keywords: Chile ; management ; Rhodophyta ; Sarcothalia ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The present paper describes growth dynamics in a natural bed of the resource ‘luga negra’ (Sarcothalia crispata) in Guapilinao, southern Chile (41°57′ S, 73°31′ W). This resource is currently harvested and exported as raw material for the production of carrageenan. Seasonal variation in biomass, frond size, density and phenology was determined by periodic sampling. Natural recruitment was evaluated on different substrata added to the field; at the same time, substrata were inoculated under greenhouse conditions. Results showed that ‘luga negra’ has seasonal growth: biomass increased from a minimum in spring to a maximum in mid to late summer. On the other hand, density was minimal in winter (200 ind. m−2) and increased to 2000 ind. m−2 in late spring. Peak abundance of mature tetrasporic fronds occurred in late summer, whereas that of cystocarpic fronds occurred in winter. Recruitment began in summer and extended into winter. Survival on different substrata were compared. Gametophytes had better survival rates on clam shells and 5 mm rope while tetrasporophytes had the best survival rate on clam shells and secondarily on boulders.
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  • 36
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    Hydrobiologia 326-327 (1996), S. 259-265 
    ISSN: 1573-5117
    Keywords: biomass-density relationship ; Gigartinaceae ; Mazzaella cornucopiae ; seaweed ; self-thinning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The negative relationship between stand biomass and plant density observed in terrestrial plants was tested among fronds of a clonal red alga, Mazzaella cornucopiae. Stand biomass and frond density were estimated bimonthly for 1 year on 7 permanent quadrats. A positive linear correlation was found between biomass and density for the whole data set, suggesting the lack of self-thinning among fronds of this intertidal alga at natural densities. Higher frond densities could favor increased water retention among fronds, thus minimizing desiccation during low tides. In this way, stands could maintain higher production of biomass. Fronds may also be cushioned better against wave action at higher frond densities, thus decreasing the detachment of larger fronds. The temporal variation of the relationship between biomass and density was plotted separately for these 7 quadrats. Four quadrats showed positive linear correlation between both variables (the other three quadrats showed non-significant positive linear correlation). Their four slopes are statistically similar to that found for the entire data set. It is possible, then, that there is only one positive slope for the biomass-density relationship in this population. If this is true, standing biomass could be estimated from the density of fronds.
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  • 37
    ISSN: 1573-5117
    Keywords: Antarctica ; biomass ; Iridaea cordata ; Rhodophyta ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Results of a seasonal study on biomass in an infralittoral population of Iridaea cordata from Terra Nova Bay (Ross Sea, Antarctica) are reported. Thalli were collected during the IX Italian Antarctic Expedition (austral summer 1993–94). The population studied is that living at depths of 4 to 6 m, where the highest density of plants occurred. The highest value of biomass (wet weight 3440 g m−2) was found at the beginning of summer. In that period 72.5% of biomass was from 128 specimens belonging to weight classes 8 (〉16 to 32 g) to 10 (〉64 g), corresponding to 13.4% of the population in numbers. Small (〈1 g) and medium (1 to 8 g) specimens provided the remaining biomass of 5% and 22.5%, respectively. During the month of January, the number of heavy specimens decreased. At the end of that month biomass reached a minimum of 2225 g m−2. In February the biomass increased to 3169 g m −2, 72% of which was from 120 specimens belonging to weight classes 7 (〉8 to 16 g) to 9 (〉32 to 64 g), which numerically represented 18.5% of the population. Data showed that biomass depended mainly on the presence of large heavy specimens, even though they were always few in number. Moreover, the occurrence of such large thalli at the beginning of summer suggests that Iridaea cordata continues to grow during the long antarctic winter.
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  • 38
    ISSN: 1573-5117
    Keywords: Exxon Valdez ; Fucus ; intertidal ; oil spill ; pollution ; restoration ; rockweed ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Exxon Valdez oil spill in March 1989 and subsequent cleanup caused injury to intertidal Fucus gardneri populations especially in the upper intertidal. A survey in 1994 in Prince William Sound, Alaska showed that the upper boundary of Fucus populations at oiled sites was still an average of 0.4 m lower than the upper boundary at unoiled sites. Restoration of severely damaged Fucus populations was started on a small-scale at a heavily oiled rocky site in Herring Bay, Prince William Sound. Experiments employed mats of biodegradable erosion control fabric to act as a substratum for Fucus germlings and to protect germlings from heat and desiccation stress. A series of plots was covered with mats made from a resilient coconut-fiber fabric in June 1993. Half of the mats were inoculated with Fucus zygotes. A series of uncovered control plots was also monitored. There was no enhancement of Fucus recruitment on the rock surfaces under the mats. Dense populations of Fucus developed on the surface of all of the mats by the summer of 1994. The natural rock surfaces in the control plots, both inoculated and not, were barren of macroscopic algal cover. By September 1994, the juvenile thalli on the mats were approximately 2 cm in length. Inoculating the mats had an effect only in the upper region of the intertidal. It is expected that the thalli will become fertile during the 1995 season. These thalli may serve as a source of embryos to enhance the recovery of new Fucus populations in this high intertidal area.
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  • 39
    ISSN: 1573-5117
    Keywords: biotest ; brackish ; Gracilaria ; growth inhibition ; marine ; photosynthesis ; Rhodophyta ; seaweed ; toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A growth inhibition test method was developed using the macroalga Gracilaria tenuistipitata as the test organism. This alga was chosen because of its high laboratory growth rates, commonly 30–40% d−1, which are reached in salinities between 5 and 40‰, and its epiphyte resistance. The toxicity of a number of substances, including heavy metals, herbicides and complex wastewaters towards the alga was assayed. Anti-fouling paints were tested with a modification of the method. EC50 values for heavy metals varied between 0.05 and 17 mg l−1 and for herbicides between 0.002 and 0.02 mg l−l. The sensitivity to the toxicant was generally higher at low salinity. Omitting nitrogen and phosphorus additions to the test medium increased the sensitivity and a semi-static performance was possible with maintained or increased sensitivity. Preliminary tests done with a computerised photosynthesis inhibition method produced promising results. In conclusion, this is a simple, sensitive and reproducible test method for assessing the toxicity of substances, wastewaters and anti-fouling paints in brackish and marine environments.
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  • 40
    ISSN: 1573-5117
    Keywords: Chondrus crispus ; carbon demand ; pH regulation ; seaweed ; tank cultivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The injection of exogenous carbon into intensively cultivated algal tanks is necessary to insure a maximum growth rate by stabilizing the dissolved inorganic carbon (DIC) pool, but represents the major part of the cultivation cost (ca. 73%). This study was conducted in paddle-wheel tanks ranging in size from 260 m2 to 1000 m2. Additional carbon was provided by carbon dioxide mixed into the incoming sea water through a tubular reactor. Production vs pH was analysed on 120 growth measurements covering two years of continuous cultivation. Whereas production peaked at pH 8.0–8.2, the economic optimum for pH regulation was in the range 8.4–8.5, where CO2 injection was greatly reduced (−29%) for only a slight decrease in production (−4%). Expressed as a function of pH level, the specific carbon injection (g c gdw−1 of Chondrus produced) showed an inverse exponential relationship, whereas gross photoconversion ratio (gdw mol photons−1) varied according to a second degree equation with a low amplitude. The photoconversion ratio was not improved when the culture was maintained at a DIC concentration higher than the natural equilibrium (0.64 ± 0.11 gdw mol photons−1 at 2.35 mM and 0.65 ± 0.15 gdw mol photons−1 at 3.19 MM). A complementary source of carbon was found in underground salt water with a high and stable DIC concentration (10.15 ± 0.25 mmole Cl−1). The mixing of the well water with natural sea water allowed another economy of CO2 (−20% at pH 8.5) and nutrients (−12%), the total unitary cost of production being cut by about 17%.
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  • 41
    ISSN: 1573-5117
    Keywords: Baltic ; disease ; endophyte ; Laminaria ; Phaeophyceae ; seaweed ; Streblonema
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The brown alga Laminaria saccharina is the dominant subtidal macroalga in Kiel Bay, western Baltic. It is infected by the microscopic brown alga, Streblonema aecidioides. Infected thalli may show symptoms of Streblonema disease, i.e. alterations of blade and stipe, ranging from dark spots to heavy deformations and completely crippled thalli. Samples taken from a single locality all year round show that (i) the host population is infected at a high rate of 87±13% (SD), but that (ii) a considerable proportion of thalli containing Streblonema does not show disease symptoms, and that (iii) juvenile hosts, which mainly appear in autumn, are infected at almost the same rate. Thus the infection seems to occur early in the host's life. Juveniles in nature show fewer symptoms of the disease than adults. Two months after infection, oxygen production and growth in laboratory-raised experimentally infected juvenile hosts was not different from uninfected controls. Experimental thalli showed more severe morphological alterations than uninfected controls only four months after infection. Both field and laboratory observations indicate that a lag phase exists between infection and outbreak of the disease.
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  • 42
    ISSN: 1573-5117
    Keywords: agar ; Gracilaria changii ; mariculture ; reproduction ; seasonality ; seaweed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gracilaria changii, recorded from Malaysia and Thailand, is one of the more abundant agarophytic seaweeds found in Malaysia. A wild population of Gracilaria changii growing in mangroves was monitored for seasonal variation in agar content and gel strength as well as spore production. Agar yield and gel strength ranged from 12 to 25% dry weight and 294 to 563 g cm−2, respectively, over a 15-month period. Gel strength but not yield was positively correlated with amount of rainfall. Cystocarps were observed throughout the study, but use of sporetraps showed that spore release peaked around July–August and January–February after the two monsoon periods. Mariculture of Gracilaria changii in shallow ponds in the mangroves, in an irrrigation canal and in a shrimp farm pond was conducted. Average growth rate of cuttings tied to monofilament lines was 3.3 ± 1.7% d−1, 8.4 ± 1.8% d−1 and 3.6 ± 1.6% d−1 respectively. Cuttings were lost to siltation, wave action, predation and heavy epiphytisation. These studies show that Gracilaria changii has potential commercial application in the agar industry.
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  • 43
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    Hydrobiologia 326-327 (1996), S. 361-365 
    ISSN: 1573-5117
    Keywords: culture ; Gelidiaceae ; Gelidium sclerophyllum ; seaweed ; vegetative propagation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Results of the first phase of production of "seed" for a project of marine culture of species of Gelidiales are presented. Vegetative fragments of different sizes and from different parts of the thallus of Gelidium sclerophyllum from the Mexican tropical Pacific coast were cultured under nine treatments with different N and P concentrations. In treatments without N all fragments died; good vegetative growth was present in all remaining treatments. Growth in length and branch production were favored in medial fragments, and rhizoid production was favored in apices. Growth in length and biomass increase were stimulated by high N concentrations (2–3 mM), and branch and rhizoid production were stimulated by high P concentrations (100–150 µM).
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  • 44
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    Hydrobiologia 116-117 (1984), S. 563-567 
    ISSN: 1573-5117
    Keywords: seaweed ; Carrageenan ; Eucheuma ; structure ; enzymatic analysis
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  • 45
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    Hydrobiologia 116-117 (1984), S. 572-575 
    ISSN: 1573-5117
    Keywords: seaweed ; porphyran ; agarases ; 13C-NMR
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    Topics: Biology
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  • 46
    ISSN: 1573-5117
    Keywords: seaweed ; Eucheuma gelatinae ; carrageenan ; viscosity ; electron microscopy
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  • 47
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    Hydrobiologia 116-117 (1984), S. 576-579 
    ISSN: 1573-5117
    Keywords: seaweed ; agarases ; Pseudomonas atlantica
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  • 48
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    Hydrobiologia 116-117 (1984), S. 580-583 
    ISSN: 1573-5117
    Keywords: seaweed ; Ulva ; urease ; inhibition ; hydroxyurea ; hydroxamic acid
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  • 49
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    Hydrobiologia 116-117 (1984), S. 584-587 
    ISSN: 1573-5117
    Keywords: seaweed ; algae ; Porphyra ; glutamate dehydrogenase ; NADP-dependent ; enzyme properties
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  • 50
    ISSN: 1573-5117
    Keywords: seaweed ; biliprotein ; phycocyanin ; phycoerythrin ; spectroscopy ; denaturation
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  • 51
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    Hydrobiologia 116-117 (1984), S. 594-596 
    ISSN: 1573-5117
    Keywords: seaweed ; R-phycocyanin ; molecular weight ; isoelectric point ; absorption spectra ; fluorescence spectra
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  • 52
    ISSN: 1573-5117
    Keywords: seaweed ; Phaeophyceae ; polyphloroglucinols ; physodes ; tannins
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  • 53
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    Hydrobiologia 116-117 (1984), S. 603-605 
    ISSN: 1573-5117
    Keywords: seaweed ; chloroplast DNA ; kelp ; restriction endonucleases ; gel electrophoresis ; autoradiography
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  • 54
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    Hydrobiologia 116-117 (1984), S. 55-58 
    ISSN: 1573-5117
    Keywords: seaweed ; Gracilaria ; geographical distribution ; western Atlantic ; red algae
    Source: Springer Online Journal Archives 1860-2000
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    Hydrobiologia 116-117 (1984), S. 59-62 
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    Keywords: seaweed ; Gracilaria ; taxonomy ; systematics ; marine algae ; morphology
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    Keywords: seaweed ; Gracilaria ; reproduction ; abundance ; seasonality ; Caribbean marine algae
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    Hydrobiologia 116-117 (1984), S. 72-76 
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    Keywords: seaweed ; Gracilaria ; raft cultivation ; growth rate ; planting time ; algal weeds
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    Hydrobiologia 116-117 (1984), S. 77-89 
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    Keywords: seaweed ; Gracilaria ; marine plants ; production ecology ; resource management ; marine algae
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    Hydrobiologia 116-117 (1984), S. 89-94 
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    Keywords: seaweed ; Gracilaria ; photosynthesis ; mariculture ; agar ; carbon limitation
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    Hydrobiologia 116-117 (1984), S. 95-101 
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    Keywords: seaweed ; Porphyra ; cultivation ; preservation ; pigments ; vitamin C
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    Hydrobiologia 116-117 (1984), S. 135-140 
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    Keywords: seaweed ; green algae ; antibiotics ; cytotoxic compounds ; bioactive terpenoids ; Udoteaceae ; Chlorophyta
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    Hydrobiologia 116-117 (1984), S. 149-152 
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    Keywords: seaweed ; hypocholesterolemic effect ; Monostroma nitidum ; feeding experiment ; plasma cholesterol ; fractionation ; β-homobetaine
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    Hydrobiologia 116-117 (1984), S. 152-154 
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    Keywords: seaweed ; economic marine algae ; herbal medicine
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    Hydrobiologia 116-117 (1984), S. 155-158 
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    Keywords: seaweed ; GABA-mimetic molecules ; red algae ; Porphyra ; metamorphic inducers ; molluscan larvae
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    Hydrobiologia 116-117 (1984), S. 158-168 
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    Keywords: seaweed ; algae ; antibiotics ; biologically active compounds
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    Hydrobiologia 116-117 (1984), S. 168-171 
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    Keywords: seaweed ; bromophenol ; diterpenes ; Dictyota indica ; dictyotriol A ; dictyotriol B
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    Hydrobiologia 116-117 (1984), S. 171-174 
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    Keywords: seaweed ; agar ; agarose ; Gracilaria ; porphyran
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    Hydrobiologia 116-117 (1984), S. 175-178 
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    Keywords: seaweed ; carrageenans ; life history phase ; antibodies ; 13C-NMR spectroscopy ; IR spectroscopy ; Gigartinaceae ; Phyllophoraceae
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    Hydrobiologia 116-117 (1984), S. 178-186 
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    Keywords: seaweed ; carrageenan ; optical rotation ; viscosity ; light scattering ; chain conformation ; polysaccharide
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  • 70
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    Keywords: seaweed ; Acetabularia ; Boergesenia ; Laurencia ; Cystoseira ; radioecology ; tritium
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  • 71
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    Keywords: seaweed ; Acetabularia ; marine alga ; apicobasal gradient ; chloroplast differentiation ; DNA synthesis
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    Keywords: seaweed ; Acetabularia ; red and blue light ; rRNA synthesis ; rRNA migration ; transcellular potential
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    Hydrobiologia 116-117 (1984), S. 197-200 
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    Keywords: seaweed ; Acetabularia calyculus ; culture ; cysts ; Dasycladaceae ; gametes
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    Hydrobiologia 116-117 (1984), S. 201-208 
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    Keywords: seaweed ; conchocelis ; endolith ; Rhodophyta ; culture ; development ; taxonomy ; morphology ; influence of substrate mineralogy ; morphometry
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    Hydrobiologia 116-117 (1984), S. 209-212 
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    Keywords: seaweed ; conchocelis ; Porphyra ; comparative morphology ; cell types ; chromatophores
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    Hydrobiologia 116-117 (1984), S. 213-217 
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    Keywords: seaweed ; carpospore ; carposporangia ; fertilization canal ; Porphyra ; prototrichogyne ; spermatium
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  • 77
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    Keywords: seaweed ; cell wall thickenings ; Chondria ; Husseyella ; algal taxonomy
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    Hydrobiologia 116-117 (1984), S. 229-232 
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    Keywords: seaweed ; Ulva ; taxonomy ; phenology
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    Hydrobiologia 116-117 (1984), S. 233-236 
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    Keywords: seaweed ; Gloeophycus koreanum ; Rhodophyta ; life history ; culture
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    Hydrobiologia 116-117 (1984), S. 243-245 
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    Keywords: seaweed ; Eucheuma ; coral reefs ; commercial cultivation ; China
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    Hydrobiologia 116-117 (1984), S. 246-2481 
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    Keywords: seaweed ; Gracilaria ; phycocolloid ; agar
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    Hydrobiologia 116-117 (1984), S. 237-242 
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    Keywords: seaweed ; Eucheuma ; agar ; distribution ; annual production
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    Hydrobiologia 116-117 (1984), S. 249-251 
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    Keywords: seaweed ; Gracilaria ; G. debilis ; G. domingensis ; seamoss ; cultivation ; Caribbean
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    Hydrobiologia 116-117 (1984), S. 252-254 
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    Keywords: seaweed ; marine algae ; cultivation ; seasonal growth ; depth ; planting density ; Gracilaria
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    Hydrobiologia 116-117 (1984), S. 255-258 
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    Keywords: seaweed ; Porphyra ; cultivation ; monospores ; light intensity
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    Hydrobiologia 116-117 (1984), S. 276-281 
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    Keywords: seaweed ; Enteromorpha ; sewage treatment ; nutrient accumulation ; growth ; effect of salinity ; light and temperature
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    Hydrobiologia 116-117 (1984), S. 29-40 
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    Keywords: seaweed ; algae ; pharmaceutical activity ; chemical constituents ; drugs
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  • 88
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    Keywords: seaweed ; microtubule assembly ; antitumor activity ; tubulin ; colchicine ; vinblastine ; Phaeophyceae
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    Hydrobiologia 116-117 (1984), S. 288-291 
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    Keywords: seaweed ; Hypneaa ; Chondruss ; cultivation ; nitrogen assimilation
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  • 90
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    Keywords: seaweed ; antifouling ; crustose coralline algae ; grazing activity ; Rhodophyceae ; scanning electron microscopy ; sea urchin
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    Hydrobiologia 116-117 (1984), S. 363-370 
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    Keywords: seaweed ; nitrogen uptake ; phosphorus uptake ; macroalgae ; seasonal fluxes ; Baltic Sea ; functional groups
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    Notes: Summary The budget calculations showed that: - the high uptake rates by the annual macroalgae, which have comparatively low biomasses, make this group play the greatest role in the total macroalgal uptake. The much higher biomasses of the perennials do not compensate for their lower uptake rates. - in spite of the decreased nutrient concentrations in late spring and summer, the total macroalgal uptakes are still high mostly thanks to the then increased biomasses of the annuals. - for all macroalgae, NH 4 + contributes about half of the N taken up during late spring to midautumn. - the perennials have their main period of nutrient uptake during late autumn and winter.
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    Hydrobiologia 116-117 (1984), S. 521-5241 
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    Keywords: seaweed ; Dictyotales ; Phaeophyceae ; antifungal ; antibacterial
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    Hydrobiologia 116-117 (1984), S. 19-28 
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    Keywords: seaweed ; alginate ; carrageenan ; gel ; ion binding ; osmometry ; viscometry ; light scattering ; multinuclear NMR ; macromolecular conformations ; polysaccharides
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  • 94
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    Keywords: seaweed ; cultivation ; Chondruss crispus ; carrageenan ; environmental simulation ; growth prediction
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    Hydrobiologia 116-117 (1984), S. 295-298 
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    Keywords: seaweed ; aeration ; carbon dioxide ; Gracilaria ; mariculture ; nutrients ; water exchange
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    Hydrobiologia 116-117 (1984), S. 299-302 
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    Keywords: seaweed ; farm ; film ; deserts ; economics ; boundary layer
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    Hydrobiologia 116-117 (1984), S. 303-307 
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    Keywords: seaweed ; red algae ; growth characteristics ; cultures
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    Hydrobiologia 116-117 (1984), S. 7-18 
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    Keywords: seaweed ; phycoculture ; China ; Laminaria ; Porphyra ; conchocelis ; Sargassum ; alginate
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    Topics: Biology
    Notes: Abstract The term ‘seaweed industry’ is employed in a broad sense and includes production both of commercial seaweeds such as Laminaria and Porphyra by phycoculture and of processed seaweed products, such as algin, agar and carrageenan. Before the founding of the People's Republic, China had a very insignificant seaweed industry, producing small quantities of the purple laver Porphyra and the glueweed Gloiopeltis by the primitive rock-cleaning method and the kelps Laminaria and Undaria by the primitive stone-throwing method, both aiming at enhancing the growth of the wild seaweeds. Also, a small quantity of agar was manufactured by the traditional Japanese method of gelling, freezing, thawing and drying the product. The small production was not sufficient to meet the demand of the Chinese people who for ages have appreciated seaweeds and their products for food. Therefore, large quantities of seaweeds and seaweed products had to be imported from various countries, for instance, Eucheuma and Gracilaria from Indonesia and other southeastern Asian countries, Laminaria and agar from Japan, even Porphyra from the USA. Annual Laminaria import from Japan generally amounted to over 10 000 tons and in some years approached 20 000–30 000 tons. Some quantities of the glueweed Gloiopeltis and the vermifuge weed Digenea simplex were exported, mainly to Japan. Since the founding of the People's Republic of China in October, 1949, China has exerted efforts to build up a self-supporting seaweed industry. Now after a lapse of 30-some years, a sizable seaweed industry has been developed. China is now able to produce by phycoculture more than one million tons of fresh seaweeds, including Laminaria, Undaria, Porphyra, Eucheuma, Gracilaria etc. and several thousand tons of seaweed extracts, including algin, agar, carrageenan, mannitol and iodine. At present, China still imports some quantities of seaweeds and seaweed products from various countries but is able to produce sufficient quantities to meet the people's need and even to export some quantities of the seaweeds Laminaria, Undaria and Porphyra and the seaweed products algin and mannitol. At the Tenth International Seaweed Symposium, I presented a paper on the Marine Phycoculture of China, in which I emphasized on the methods of cultivation (Tseng 1981b). Therefore I would like to take this opportunity to supplement the last lecture by presenting a paper on the role of phycological research in the development of China's seaweed industry.
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    Hydrobiologia 116-117 (1984), S. 308-313 
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    Keywords: seaweed ; Porphyra ; cell-separation ; propagation ; aquaculture
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    Hydrobiologia 116-117 (1984), S. 314-316 
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    Keywords: seaweed ; somatic cells ; clone ; callus ; totipotency ; dedifferentiation
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