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  • 1995-1999  (5)
  • 1980-1984  (4)
  • 1975-1979
  • Nuclear reactions
  • Recombinant DNA
  • 1
    Digitale Medien
    Digitale Medien
    Strategies for gene cloning (1999)
    Springer
    Urological research 27 (1999), S. 83-96 
    Details
    ISSN: 1434-0879
    Schlagwort(e): Key words Genitourinary malignancies ; Recombinant DNA ; Gene cloning ; DNA chip technology ; Diagnostics ; Gene therapy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Cancer, including genitourinary malignancy, is a consequence of accumulated genetic aberrations in genes involved in crucial regulatory pathways. The result is a deregulation of cellular behaviour, leading to neoplastic transformation, uncontrolled cell proliferation and acquisition of metastatic ability. The development and perfection of techniques in the field of recombinant DNA technology, gene cloning, (differential) analysis of gene expression, and sequencing of genes and proteins have provided a wealth of information about the genetic aberrations associated with cancer development. This “recombinant DNA and gene cloning” technology and the recently developed DNA chip technology may provide new molecular diagnostic tools. Furthermore, the technology of gene cloning in combination with the progress in in vivo gene delivery techniques offers new treatment modalities, like gene therapy, additional to conventional therapies. This review is intended to provide a general introduction to the fundamentals or strategies of recombinant DNA and gene cloning techniques as a basis for understanding the rapidly expanding range of new diagnostic and therapeutic opportunities. Some illustrative examples are provided, addressing basic and biomedical research and possible clinical applications in genitourinary oncology.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002400050093
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  • 2
    Digitale Medien
    Digitale Medien
    Cloning and expression of the Apa LI, Nsp I, Nsp HI, Sac I, Sca I, and Sap I restriction-modification systems in Escherichia coli (1998)
    Springer
    Molecular genetics and genomics 260 (1998), S. 226-231 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Restriction endonuclease ; Methylase selection ; Gene expression ; DNA methylation ; Recombinant DNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The genes encoding the ApaLI (5′-G^TGCAC-3′), NspI (5′-RCATG^Y-3′), NspHI (5′-RCATG^Y-3′), SacI (5′-GAGCT^C-3′), SapI (5′-GCTCTTCN1^-3′, 5′-^N4GAAGAGC-3′) and ScaI (5′-AGT^ACT-3′) restriction-modification systems have been cloned in E.␣coli. Amino acid sequence comparison of M.ApaLI, M.NspI, M.NspHI, and M.SacI with known methylases indicated that they contain the ten conserved motifs characteristic of C5 cytosine methylases. NspI and NspHI restriction-modification systems are highly homologous in amino acid sequence. The C-termini of the NspI and NlaIII (5′-CATG-3′) restriction endonucleases share significant similarity. 5mC modification of the internal C in a SacI site renders it resistant to SacI digestion. External 5mC modification of a SacI site has no effect on SacI digestion. N4mC modification of the second base in the sequence 5′-GCTCTTC-3′ blocks SapI digestion. N4mC modification of the other cytosines in the SapI site does not affect SapI digestion. N4mC modification of ScaI site blocks ScaI digetion. A DNA invertase homolog was found adjacent to the ApaLI restriction-modification system. A DNA transposase subunit homolog was found upstream of the SapI restriction endonuclease gene.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004380050890
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  • 3
    Digitale Medien
    Digitale Medien
    Mixing in Stars and the Evolution of the 3He Abundance (1998)
    Springer
    Space science reviews 84 (1998), S. 199-206 
    Details
    ISSN: 1572-9672
    Schlagwort(e): Nuclear reactions ; Nucleosynthesis ; Abundances ; Stars:Evolution ; Interior ; Rotation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract We first recall the observational and theoretical facts that constitute the so-called 3He problem. We then review the chemical anomalies that could be related to the destruction of 3He in red giants stars. We show how a simple consistent mechanism can lead to the destruction of 3He in low mass stars and simultaneously account for the low 12C/13C ratios and low lithium abundances observed in giant stars of different populations. This process should both naturally account for the recent measurements of 3He/H in galactic HII regions and allow for high values of 3He observed in some planetary nebulae. We propose a simple statistical estimation of the fraction of stars that may be affected by this process.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1005027519798
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  • 4
    Digitale Medien
    Digitale Medien
    Norms for patents concerning human and other life forms (1996)
    Springer
    Theoretical medicine and bioethics 17 (1996), S. 279-314 
    Details
    ISSN: 1573-1200
    Schlagwort(e): Recombinant DNA ; transgenesis ; patents ; human life forms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Philosophie
    Notizen: Abstract The rationale of patents on transgenic organisms leads to the startling notion of the human qua infringement. The moral reasons by which we may tenably reject such notion are not conclusive as to human life forms outside the body. A close look at recombinant DNA experimentation reveals ingenious processes, but not entities that the body lacks. Except for artificial genes, the genes of biotechnology are found on chromosomes, albeit nonconsecutively, and their uninterrupted transcripts appear in messenger RNA. An enhanced form of protection for ingenious processes, the “human methods patent,” is proposed and defended as a replacement for product patents. The proposed patent would pertain to biotechnology manufacturing and genetic intervention in somatic and germ cells. A counterpart could govern nonhuman life forms. It is argued that compulsory licensing protections should be a condition of such patent. Contrary to the conservative assumption that statutory sobriquets suffice, the reckoning of what qualifies as a patentable ingenious process will continue to require systematic scientific guidance.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00489450
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  • 5
    Digitale Medien
    Digitale Medien
    A novel strategy for the isolation of defined pyrG mutants and the development of a site-specific integration system for Aspergillus awamori (1995)
    Springer
    Current genetics 27 (1995), S. 536-540 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Recombinant DNA ; Filamentous fungi ; 5-fluoro-orotic acid ; Homologous transformation system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A homologous gene transfer system for Aspergillus awamori for site-specific integration is described, based on two components. First, a defined A. awamori pyrG mutant strain constructed by a selection strategy for gene-replacement in fungi. Second, a vector with a homologous pyrG selection marker containing a defined mutation at a site different from that of the mutations in the pyrG gene of the defined mutant strain. Defined mutation in the A. awamori pyrG gene, isolated from a genomic library by heterologous hybridisation with the A. niger pyrG gene as a probe, were introduced by specifically altering sequences at restriction sites in the coding region of the gene. After transformation of the A. awamori wild-type strain with vectors containing these mutated pyrG genes, and selection for 5-fluoro-orotic acid resistance (5-FOAR), on the average 60% of the 5-FOAR colonies originated from replacement of the wild-type pyrG gene by the mutated pyrG allele. After transformation of a mutant strain, carrying a mutation near the 5′ end of the pyrG gene with vectors containing a mutation near the 3′ end of the pyrG gene, 35% of the resulting transformants contained one copy of the vector at the pyrG locus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00314444
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  • 6
    Digitale Medien
    Digitale Medien
    Cloning of the ribokinase gene of Staphylococcus hyicus subsp. hyicus in Staphylococcus carnosus (1984)
    Springer
    Archives of microbiology 140 (1984), S. 218-224 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Recombinant DNA ; Cloning ; Staphylococcus carnosus and S. hyicus subsp. hyicus ; Ribose degradation ; Ribokinase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A gene library with DNA of Staphylococcus hyicus subsp. hyicus was established in S. carnosus by using the plasmid vector pCT20. Two clones of S. carnosus were isolated which were able to ferment d-ribose. The two hybrid plasmids (pRib 1) and (pRib 2) were isolated and characterized. They contained inserted DNA fragments of S. hyicus subsp. hyicus with sizes of 10.2 and 8.2 kb, respectively. d-Ribose uptake and enzyme activities were studied. All strains tested [S. hyicus subsp. hyicus, S. carnosus (wild type) and the two S. carnosus clones] possessed an inducible uptake system for d-ribose. S. hyicus subsp. hyicus possessed in addition enzyme activities of d-ribokinase and d-ribose-5-P isomerase. None of these enzyme activities could be detected in S. carnosus (wildtype). Only in the S. carnosus clones containing (pRib 1) or (pRib 2) could a d-ribokinase activity be demonstrated, indicating that the gene for d-ribokinase of S. hyicus subsp. hyicus was cloned in S. carnosus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00454931
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  • 7
    Digitale Medien
    Digitale Medien
    Cloning and analysis of recombinant plasmids containing genes for Aspergillus nidulans 5 S rRNA (1981)
    Springer
    Current genetics 4 (1981), S. 173-176 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Aspergillus ; 5S rRNA ; Recombinant DNA ; Restriction Analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genes coding for 5S rRNA were found to be dispersed in the Aspergillus nidulans genome. Three different recombinant plasmids hybridizing to 5S rRNA were isolated and their restriction enzyme maps were established.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420494
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  • 8
    Digitale Medien
    Digitale Medien
    Cloned ß-galactosidase gene of Escherichia coli is expressed in the yeast Saccharomyces cerevisiae (1980)
    Springer
    Current genetics 2 (1980), S. 109-113 
    Details
    ISSN: 1432-0983
    Schlagwort(e): Recombinant DNA ; 2 µm DNA ; Heterologous gene expression ; Eukaryotic host
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The expression of the lacZ gene of E. coli in S. cerevisiae has been studied. The enzymatic activity coded by the lacZ gene in E. coli, β-galactosidase, is detectable in yeast cells harboring a chimeric plasmid carrying the gene. On the basis of size and immunological criteria, no difference was detected between the Coli-in-yeast β-galactosidase and the E. coli enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00420622
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  • 9
    Digitale Medien
    Digitale Medien
    Use of an (n, α) nuclear reaction to study the long-distance transport of boron in trifolium repens after foliar application (1980)
    Springer
    Planta 150 (1980), S. 197-205 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Boron ; Foliar nutrition ; Nuclear reactions ; Transport (boron) ; Trifolium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The growth of white clover (Trifolium repens L.) is severely inhibited by boron starvation, but a foliar treatment with boric acid can transitorily alleviate the deficiency symptoms. The 10B(n ,α)7 Li nuclear reaction has been used to study boron transport in the plant after foliar application. More than 98% of the boron supplied remained at the point where it was applied to the leaves, and less than 2% was useful to the growth of the treated plant. This small “efficient” portion of boron was quite mobile. It was distributed to the different parts of the plant, then was transferred from the oldest parts to the newly formed leaves. Physiological and agronomical implications of these data are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00390826
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