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  • 2000-2004  (8)
  • 1990-1994  (9)
  • 1955-1959  (4)
  • 1940-1944
  • particle bombardment
  • 1
    Digitale Medien
    Digitale Medien
    Transformation of the tropane alkaloid-producing medicinal plant Hyoscyamus muticus by particle bombardment (2000)
    Springer
    Transgenic research 9 (2000), S. 163-168 
    Details
    ISSN: 1573-9368
    Schlagwort(e): Hyoscyamus muticus ; particle bombardment ; transformation ; tropane alkaloids
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We report an efficient whole plant transformation system for Hyoscyamus muticus, an important medicinal plant of the Solanaceous family. We developed a system using a plasmid carrying the nptII and gusA genes, which was delivered into leaf explants by particle bombardment. Ten percent of bombarded leaf explants formed kanamycin-resistant callus, from which putative transgenic plants were recovered. The nptII gene conferring kanamycin resistance was found to be incorporated into the genome of all transgenic plants screened. Over 50% of the kanamycin resistant plants showed strong expression of the non-selected gusA gene. The majority of transgenic plants reached maturity, could be self pollinated, and produced fertile seed. A simple and efficient whole plant transformation system for this medicinal plant is an important step in furthering our understanding of tropane alkaloid production in plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008912330067
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  • 2
    Digitale Medien
    Digitale Medien
    Direct transformation and plant regeneration of the haploid liverwort Marchantia polymorpha L. (2000)
    Springer
    Transgenic research 9 (2000), S. 179-185 
    Details
    ISSN: 1573-9368
    Schlagwort(e): direct transformation ; haploid plant ; Marchantia polymorpha ; particle bombardment ; plant regeneration ; stable integration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Thalli of the haploid liverwort Marchantia polymorpha were successfully used for direct particle bombardment with plasmid pMT, which carries a hygromycin phosphotransferase gene (hpt) controlled by the CaMV 35S promoter and the NOS polyadenylation region. Hygromycin-resistant cell masses arose from the thallus surface and developed directly into hygromycin-resistant thalli. Southern blot analyses indicated that these thalli carried at least 1–4 copies of the hpt gene, which were stably transmitted to their asexual thallus progenies via gemma propagation for three generations. This transformation and direct plant regeneration protocol is expected to be a valuable tool for the molecular analysis of this lower land plant.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008963410465
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  • 3
    Digitale Medien
    Digitale Medien
    Enhanced insect resistance in Thai rice varieties generated by particle bombardment (2000)
    Springer
    Molecular breeding 6 (2000), S. 391-399 
    Details
    ISSN: 1572-9788
    Schlagwort(e): cotransformation ; gna gene ; insect resistance ; Oryza sativa L. ; particle bombardment ; transgenic Thai rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract We used particle bombardment to transform two elite Thai rice varieties, Khao Dawk Mali 105 (KDML105) and Supanburi 60 (SP60), with the snowdrop lectin gene gna (Galanthus nivalis agglutinin). This gene confers resistance to sap-sucking insects such as the brown planthopper (BPH; Nilaparvata lugens), which is one of the most damaging pests of rice. Traditionally, KDML105 and SP60 have been regarded as recalcitrant to transformation, and this is the first account of successful gene transfer to these varieties. By molecular analysis, we confirmed the recovery of over thirty gna-transgenic lines. GNA protein expression was characterised by western blot analysis, and we achieved expression levels of up to 0.25% total soluble protein. GNA-producing R1 transgenic plants were significantly more resistant to BPH than control plants (P〈0.0001), with 37% and 42% reduction in nymphal survival for constitutive and phloem-specific expression, respectively. Transferring the gna gene to these superior rice varieties thus represents a major step forward for crop improvement in Thailand, and should help to reduce the damage caused by rice pests, and hence increase yields for this vital domestic and export market.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1009633703157
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  • 4
    Digitale Medien
    Digitale Medien
    Cereal crops as viable production and storage systems for pharmaceutical scFv antibodies (2000)
    Springer
    Plant molecular biology 42 (2000), S. 583-590 
    Details
    ISSN: 1573-5028
    Schlagwort(e): transgenic cereals ; molecular pharming ; antibodies ; carcinoembryonic antigen ; single-chain Fv fragment ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract This report describes the stable expression of a medically important antibody in the staple cereal crops rice and wheat. We successfully expressed a single-chain Fv antibody (ScFvT84.66) against carcinoembryonic antigen (CEA), a well characterized tumor-associated marker antigen. scFv constructs were engineered for recombinant antibody targeting to the plant cell apoplast and ER. Up to 30 μg/g of functional recombinant antibody was detected in the leaves and seeds of wheat and rice. We confirmed that transgenic dry seeds could be stored for at least five months at room temperature, without significant loss of the amount or activity of scFvT84.66. Our results represent the first transition from model plant expression systems, such as tobacco and Arabidopsis, to widely cultivated cereal crops, such as rice and wheat, for expression of an antibody molecule that has already shown efficacy in clinical applications. Thus, we have established that molecular pharming in cereals can be a viable production system for such high-value pharmaceutical macromolecules. Our findings provide a strong foundation for exploiting alternative uses of cereal crops both in industrialized and developing countries.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1006301519427
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  • 5
    Digitale Medien
    Digitale Medien
    Identification of a Novel Plant Virus Promoter using a Potyvirus Infectious Clone (2000)
    Springer
    Virus genes 20 (2000), S. 11-17 
    Details
    ISSN: 1572-994X
    Schlagwort(e): plant viral promoter ; caulimovirus ; strawberry vein banding virus ; zucchini yellow mosaic virus ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract A putative promoter from the strawberry vein banding caulimovirus (SVBV) genome was identified by its ability to drive infection with full-length cDNA of the zucchini yellow mosaic RNA potyvirus (ZYMV). A high rate of infection was obtained with the cDNA under control of the SVBV promoter using particle bombardment technology. The SVBV promoter shows 60% homology to the cauliflower mosaic virus 35S promoter in the domain spanning the conserved motifs of CCACT (at −83) and the TATA box (at −31), to the transcription start. The 3′-end one-third of the putative promoter (328 bp) was sufficient to invoke full infectivity with the ZYMV clone, and drove transient reporter gene expression in Solanaceae and Cucurbitaceae transformed with a binary plant transformation vector. Stable expression of a reporter gene (GUS) under control of the truncated SVBV promoter was shown in transformed tobacco shoots in roots, leaves and stems.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008199805099
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  • 6
    Digitale Medien
    Digitale Medien
    The effect of DNA/gold particle preparation technique, and particle bombardment device, on the transformation of barley (Hordeum vulgare) (2000)
    Springer
    Euphytica 111 (2000), S. 67-76 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Barley ; Hordeum vulgare ; transformation ; particle bombardment ; particle gun
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Immature embryos of the spring barley variety GoldenPromise, were bombarded with three different particledelivery systems and both transient and stabletransformation examined. In addition, a range oftechniques for the preparation of the DNA coated goldparticles was examined. Fertile transgenic barleyplants were obtained using three particle preparationtechniques which differed in the amount of gold andDNA used for each bombardment. However, only one ofthe particle delivery systems, the PDS 1000/He device,appeared to be effective in yielding transformedbarley plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1003700300235
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  • 7
    Digitale Medien
    Digitale Medien
    Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns (2000)
    Springer
    Transgenic research 9 (2000), S. 11-19 
    Details
    ISSN: 1573-9368
    Schlagwort(e): particle bombardment ; minimal cassette ; cotransformation ; transgenic rice ; integration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Whole plasmids are used in both Agrobacterium-mediated transformation and direct DNA transfer, generally leading to the integration of vector backbone sequences into the host genome along with the transgene(s). This is undesirable, as vector backbone sequences often have negative effects on transgene or endogenous gene expression, and can promote transgene rearrangements. We, therefore, bombarded rice tissue with two constructs: a plasmid containing the bar gene, and a linear DNA fragment isolated from the same plasmid, corresponding to the minimal bar gene expression cassette (promoter, open reading frame and terminator). We recovered phosphinothricin-resistant plants from both experiments, showing that the selectable marker was efficiently expressed. Transformation with such constructs resulted in predominantly 'simple' integration events (one or two bands on Southern blots), producing low-copy-number transgenic plants with a low frequency of transgene rearrangements. Conversely, transformation with supercoiled or linearized whole plasmids generated plants with 'complex' integration patterns, that is, higher copy numbers and frequent transgene rearrangements. We monitored transgenic lines through to the R4 generation and observed no silencing in plants carrying minimal constructs. We also carried out experiments in which rice tissue was simultaneously bombarded with minimal linear hpt and gusA cassettes. We observed robust GUS activity in hygromycin-resistant plants, confirming co-expression of the selectable and nonselectable markers. Furthermore, the efficiency of cotransformation using minimal constructs was the same as that using supercoiled plasmid cointegrate vectors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008993730505
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  • 8
    Digitale Medien
    Digitale Medien
    Overexpression of The Calcium-Dependent Protein Kinase OsCDPK2 in Transgenic Rice is Repressed by Light in Leaves and Disrupts Seed Development (2000)
    Springer
    Transgenic research 9 (2000), S. 453-462 
    Details
    ISSN: 1573-9368
    Schlagwort(e): calcium-dependent ; light regulation ; particle bombardment ; protein kinase ; seed development ; transgenic rice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Independent transgenic rice lines overexpressing the rice CDPK isoform OsCDPK2 were generated by particle bombardment. High levels of OsCDPK2 were detected in leaves removed from etiolated plants, as well as in stems and flowers. However, there was no overexpression in green leaves that had been exposed to light, confirming that OsCDPK2 protein stability was subject to light regulation. The morphological phenotype of transgenic plants producing high levels of recombinant OsCDPK2 was normal until the onset of seed development. Flowers developed normally, producing well-shaped ovaries and stigmas, and mature anthers filled with pollen grains. However, seed formation in these plants was strongly inhibited, with only 3–7% of the flowers producing seeds. Seed development was arrested at an early stage. We discuss these data with respect to the possible requirement for specific CDPK isoforms during rice seed 4.4ptdevelopment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1026555021606
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  • 9
    Digitale Medien
    Digitale Medien
    Fertile transgenic barley by particle bombardment of immature embryos (1994)
    Springer
    Plant molecular biology 24 (1994), S. 317-325 
    Details
    ISSN: 1573-5028
    Schlagwort(e): fertile transgenic barley ; gene transfer ; Hordeum vulgare ; neomycin phosphotransferase II ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Transgenic, fertile barley (Hordeum vulgare L.) from the Finnish elite cultivar Kymppi was obtained by particle bombardment of immature embryos. Immature embryos were bombarded to the embryonic axis side and grown to plants without selection. Neomycin phosphotransferase II (NPTII) activity was screened in small plantlets. One out of a total of 227 plants expressed the transferred nptII gene. This plant has until now produced 98 fertile spikes (T0), and four of the 90 T0 spikes analyzed to date contained the nptII gene. These shoots were further analyzed and they expressed the transferred gene. From green grains, embryos were isolated and grown to plantlets (T1). The four transgenic shoots of Toivo (the T0 plant) produced 25 plantlets as T1 progeny. Altogether fifteen of these T1 plants carried the transferred nptII gene as detected with the PCR technique, fourteen of which expressed the nptII gene. The integration and inheritance of the transferred nptII gene was confirmed by Southern blot hybridization. Although present as several copies, the transferred gene was inherited as a single Mendelian locus into the T2 progeny.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020170
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  • 10
    Digitale Medien
    Digitale Medien
    Stable transformation ofArabidopsis with thebar gene using particle bombardment (1994)
    Springer
    Transgenic research 3 (1994), S. 279-286 
    Details
    ISSN: 1573-9368
    Schlagwort(e): Arabidopsis thaliana ; transgenic plants ; particle bombardment ; bar gene ; phosphinothricin acetyltransferase ; late-flowering mutant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A plasmid pARK 22 harbouring thebar gene encoding phosphinothricin acetyltransferase (PAT) under the control of the cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator was constructed and introduced into root sections ofArabidopsis thaliana using the pneumatic particle gun. The root sections that had been bombarded with this plasmid gave four to eight times higher yield of drug-resistant calluses than those sections bombarded with pCaMVNEO or pCH, which respectively contain the neomycin phosphotransferase and hygromycin phosphotransferase genes. Among a number of primary transformant (T0) plants obtained from independent bialaphos-resistant calluses, three were studied by Southern blot hybridization and PAT enzyme activity analyses, confirming the stable integration of the foreign gene into theArabidopsis genome and its expression in plants. The progeny analysis showed transmission of the foreign gene and its expression in up to the T2 generation. Some of the T1 progeny showed morphological abnormalities. Thus, thebar gene can be used effectively to allow selection of transgenicA. thalianna plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01973587
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  • 11
    Digitale Medien
    Digitale Medien
    The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells (1993)
    Springer
    Plant cell reports 12 (1993), S. 506-509 
    Details
    ISSN: 1432-203X
    Schlagwort(e): particle bombardment ; Hordeum vulgare L. ; promoter screening ; transient expression ; gus ; cereal transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoterintron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00236096
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  • 12
    Digitale Medien
    Digitale Medien
    Transgenic roots produced by introducing Ri-rol genes into cucumber cotyledons by particle bombardment (1993)
    Springer
    Transgenic research 2 (1993), S. 147-152 
    Details
    ISSN: 1573-9368
    Schlagwort(e): rol genes ; transgenic root ; Cucumis sativus ; particle bombardment ; hairy root
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Transformed roots ofCucumis sativus were obtained from cotyledon tissues that had been bombarded with gold particles coated with plasmid pE7.4 using a pneumatic particle gun. This plasmid containsrolA, rolB, rolC genes and ORF 13 of the 7.4 kbEco RI fragment of T-DNA of pRi 1724 isolated fromAgrobacterium rhizogenes MAF 03-01724. The nature of the tissue and the composition of the culture media used greatly influenced the recovery of transformed roots. The transgenic nature of the derived roots was confirmed by the vigorous. highly-branched growth seen on a phytohormone-free medium. The stable integration ofrol genes into the cucumber genome was confirmed by Southern blot analysis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01972608
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  • 13
    Digitale Medien
    Digitale Medien
    Agrobacterium tumefaciens-mediated expression ofgusA in maize tissues (1993)
    Springer
    Transgenic research 2 (1993), S. 252-265 
    Details
    ISSN: 1573-9368
    Schlagwort(e): Agrobacterium tumefaciens ; maize ; GUS ; gusA/intron ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract To develop a system forAgrobacterium-mediated transformation of maize (Zea mays L.), we have investigated histochemically the transient expression of β-glucuronidase (GUS) activity in maize seedling tissue segments using binary vectors that allow minimal (pKIWI105 and pCNL1) or undetectable (p35S-GUS-INT and pCNL56) levels of GUS activity inA. tumefaciens. Tissue segments from three- to five-day-old sterile seedlings of maize genotype A188 were inoculated withA. tumefaciens. Four days after inoculation, transient expression of GUS activity was found in mesocotyl segments originating from the intercalary meristem region. This GUS activity was specific to the vascular cylinder and was not found in the internal cortical or epidermal layers, nor was it found in mature mesocotyl tissue (segments 5 mm below the coleoptilar node). Transient GUS activity was also detected in leaf and coleoptile tissues of shoot segments, but not in the shoot apexper se or in leaves younger than the first leaf. Maize tissues inoculated withA. tumefaciens strains that harbourgusA-containing binary vectors but no Ti-plasmid did not show GUS activity, supporting evidence from previous work thatvir gene activity was essential for the observed GUS activity.A. tumefaciens strains containing different types of Ti-plasmids were also tested. A strain harbouring an agropine-type Ti-plasmid was the most effective for expressing GUS activity in mesocotyl segments, whereas a strain harboring a nopaline-type Ti-plasmid was most effective for expression of GUS activity in the apical meristem-containing segment. These results indicate that different interactions occurred between the differentA. tumefaciens strains and the susceptible plant tissues. Maize genotype specificity for GUS activity in mesocotyl tissues was observed; variations in the cocultivation medium had a profound effect on the frequency of expression of GUS activity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01968838
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  • 14
    Digitale Medien
    Digitale Medien
    The biotechnology of crop legumes (1993)
    Springer
    Euphytica 74 (1993), S. 165-185 
    Details
    ISSN: 1573-5060
    Schlagwort(e): transgenic legumes ; genetic engineering ; particle bombardment ; Agrobacterium ; direct DNA transfer ; crop legumes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The absence of variety-independent gene transfer methods for major agronomic species has, until now, limited the usefulness of recombinant DNA techniques to crop improvement programs. Until recently, only Solanaceous crops could be used to study fundamental and applied problems in plant sciences. During the past five years rapid advances in cell biology, in combination with the development of novel gene transfer methodology allowed utilization of the tools of plant molecular biology in conventional breeding programs. Cereal and leguminous species were considered to be recalcitrant to genetic manipulation. As a result of the development of direct DNA transfer methodology into organized tissue, we are now in a position to introduce any foreign gene into almost all of the major cereals and legumes. This can be achieved efficiently, often in a variety-independent fashion. The object of this review is to provide a comprehensive account of the state of the art in gene transfer for the cultivated leguminous crops. Important oilseed and feed species primarily in industrialized countries, as well as minor but equally important species for sustaining growth populations in developing countries will be examined. Advantages of the various gene transfer methods that were shown to be useful for specific crops, as well as limitations and problems associated with each crop and gene transfer method will be discussed. Data from field trials of transgenic legumes, where available, will be presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040399
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  • 15
    Digitale Medien
    Digitale Medien
    Development of the Particle Inflow Gun (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 237-246 
    Details
    ISSN: 1573-5044
    Schlagwort(e): embryogenic suspension culture ; Glycine max ; particle bombardment ; stable transformation ; transient expression ; Zea mays
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A simple and inexpensive particle acceleration apparatus was designed for direct delivery of DNA to plant cells. The Particle Inflow Gun (PIG) is based on acceleration of DNA-coated tungsten particles directly in a helium steam. High levels of transient expression of theβ-glucuronidase gene were obtained following bombardment of embryogenic suspension cultures of maize and soybean, and leaf tissue of cowpea. Stable transformation of soybean and maize has also been obtained using this bombardment apparatus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02319007
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  • 16
    Digitale Medien
    Digitale Medien
    Direct DNA transfer using electric discharge particle acceleration (ACCELL™ technology) (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 227-236 
    Details
    ISSN: 1573-5044
    Schlagwort(e): dicot transformation ; monocot transformation ; particle bombardment ; transgenic cotton ; Gossypium hirsutum ; transgenic riceOryza sativa ; transgenic soybeanGlycine max
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Direct DNA transfer methods based on particle bombardment have revolutionized plant genetic engineering. Major agronomic crops previously considered recalcitrant to gene transfer have been engineered using variations of this technology. In many cases variety-independent and efficient transformation methods have been developed enabling application of molecular biology techniques to crop improvement. The focus of this article is the development and performance of electric discharge particle bombardment (ACCELL™) technology. Unique advantages of this methodology compared to alternative propulsion technologies are discussed in terms of the range of species and genotypes that have been engineered, and the high transformation frequencies for major agronomic crops that enabled the technology to move from the R&D phase to commercialization. Creation of transgenic soybeans, cotton, and rice will be used as examples to illustrate the development of variety-independent and efficient gene transfer methods for most of the major agronomic crops. To our knowledge, no other gene transfer method based on particle bombardment has resulted in variety-independent and practical generation of large numbers of independently-derived crop plants. ACCELL™ technology is currently being utilized for the routine transfer of valuable genes into elite germplasm of soybean, cotton, bean, rice, corn, peanut and woody species.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02319006
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  • 17
    Digitale Medien
    Digitale Medien
    Particle bombardment-mediated transient expression of a Brazil nut methionine-rich albumin in bean (Phaseolus vulgaris L.) (1992)
    Springer
    Plant molecular biology 20 (1992), S. 357-359 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Brazil nut ; 2S albumin gene ; gene transfer ; Phaseolus vulgaris ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Bean (Phaseolus vulgaris L.) mature embryos were transformed using biolistic methods with a plasmid containing 2S albumin and β-glucuronidase structural sequences, both under the control of the 35S CaMV promoter. We have shown that chimaeric tissues could be obtained and that both structural sequences were expressed to similar levels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00014508
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  • 18
    Digitale Medien
    Digitale Medien
    Transformation studies in Hordeum vulgare using a highly regenerable microspore system (1955)
    Springer
    Euphytica 85 (1955), S. 113-118 
    Details
    ISSN: 1573-5060
    Schlagwort(e): Hordeum vulgare ; isolated microspores ; particle bombardment ; transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A highly regenerable, isolated microspore system for barley, Hordeum vulgare L. cv. Igri, has been developed which is amenable to transformation studies using particle bombardment. The system allows DNA to be delivered to microspores at the single cell stage and both transient and stable transformation events have been demonstrated. The potential advantages of using isolated microspores as the target tissue in routine transformation systems are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023938
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  • 19
    Digitale Medien
    Digitale Medien
    Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants (1955)
    Springer
    Euphytica 85 (1955), S. 81-88 
    Details
    ISSN: 1573-5060
    Schlagwort(e): gene transfer ; Hordeum vulgare ; neomycin phosphotransferase II ; particle bombardment ; transgenic barley
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Transgenic barley plants (Hordeum vulgare L. cv. Kymppi) were obtained by particle bombardment of various tissues. Immature embryos and microspore-derived cultures were bombarded with gold particles coated with plasmid DNA carrying the gene coding for neomycin phosphotransferase II (NPTII), together with plasmid DNA containing the gene for β-glucuronidase (GUS). Bombarded immature embryos were grown to plants without selection and NPTII activity was screened in small plantlets. One plant proved to be transgenic (T0). This chimeric plant passed the transferred nptII gene to its T1 progeny. The presence of the nptII gene was demonstrated by the PCR technique and enzyme activity was analyzed by an NPTII gel assay. Four T0 spikes and 15 T1 offspring were transgenic. The integration and inheritance was confirmed by Southern blot hybridization. Transgenic T2 and T3 plants were produced by isolating embryos from green grains of transgenic T1 and T2 plants, respectively and growing them to plants. After selfing, the ratio of transgenic to non-transgenic T2 offspring was shown to follow the rule of Mendelian inheritance. The general performance of transgenic plants was normal and no reduction in fertility was observed. Microspore-derived cultures were bombarded one and four weeks after microspore isolation. After bombardment, cultures were grown either with or without antibiotic selection (geneticin R or kanamycin). When cultures were grown without selection and regenerated plants were transferred to kanamycin selection in rooting phase, one out of a total of about 1500 plants survived. This plant both carried and expressed the transferred nptII gene. The integration was confirmed by Southern blot hybridization. This plant was not fertile.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023933
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  • 20
    Digitale Medien
    Digitale Medien
    Genetic engineering of cereal crop plants: a review (1955)
    Springer
    Euphytica 85 (1955), S. 35-44 
    Details
    ISSN: 1573-5060
    Schlagwort(e): cereals ; protoplast transformation ; tissue electroporation ; particle bombardment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Many aspects of basic and applied problems in plant biology can be investigated by transformation techniques. In dicotyledonous species, the ability to generate transgenic plants provides the tools for an understanding of plant gene function and regulation as well as for the directed transfer of genes of agronomic interest. For many dicotyledonous plants Agrobacterium tumefaciens can be routinely used to introduce foreign DNA into their genome. However, cereals seem to be recalcitrant to Agrobacterium-mediated transformation. In cereals, many efforts have been made in recent years to establish reliable transformation techniques. Several transformation techniques have been developed but to date only three methods have been found to be suitable for obtaining transgenic cereals: transformation of totipotent protoplasts, particle bombardment of regenerable tissues and, more recently, tissue electroporation. The current state of transformation methods used for cereals will be reviewed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023928
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  • 21
    Digitale Medien
    Digitale Medien
    Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment (1955)
    Springer
    Euphytica 85 (1955), S. 13-27 
    Details
    ISSN: 1573-5060
    Schlagwort(e): gene transfer ; crop species ; particle bombardment ; transgenic plants ; cereals ; legumes ; woody plants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The limiting component in the creation of transgenic crops has been the lack of effective means to introduce foreign genes into elite germplasm. However, the development of novel direct DNA transfer methodology, by-passing limitations imposed by Agrobacterium-host specificity and cell culture constraints, has allowed the engineering of almost all major crops, including formerly recalcitrant cereals, legumes and woody species. The creation of transgenic rice, wheat, maize, barley, oat, soybean, phaseolus, peanut, poplar, spruce, cotton and others, in an efficient and in some cases, variety-independent fashion, is a significant step towards the routine application of recombinant DNA methodology to the improvement of most important agronomic crops. In this review we will focus on key elements and advantages of particle bombardment technology in order to evaluate its impact on the accelerated commercialization of products based on agricultural biotechnology and its utility in studying basic plant developmental processes and function through transgenesis. Fundamental differences between conventional gene transfer methods, utilizing Agrobacterium vectors or protoplast/suspension cultures, and particle bombardment will be discussed in depth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023926
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