ISSN:
1432-0428
Keywords:
Insulin receptors
;
human adipocytes
;
insulin degradation
;
lipogenesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The binding of125I-labelled insulin to human adipocytes was studied at 37° C. The precipitability of the125I-labelled insulin preparation (0.03 nmol/l) in trichloroacetic acid and the concentration of biologically active insulin (7.5 nmol/l) remained constant in buffer incubated with human adipocytes (100 μl cells/ml suspension) for 30–60 minutes at 37° C, whereas more than half of the insulin was inactivated by rat fat cells under the same conditions. A constant level of binding of125I-labelled insulin (0.03 nmol/l) to human adipocytes was obtained after 45 minutes. The apparent dissociation constant of receptor binding was about 0.2 nmol/l as compared to about 2 nmol/l for rat adipocytes. Conversion of [U-14C]glucose to lipids was stimulated half-maximally by about 0.05 nmol/l of insulin (similar to rat adipocytes). Thus, half-maximal stimulation of human adipocytes was obtained with a receptor occupancy of about 20–30 per cent.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01236312
