ISSN:
1432-0428
Keywords:
Membrane
;
cell coat
;
lectin-binding sites
;
exocytosis
;
cell web
;
B-cell
;
islet of Langerhans
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Using ferritin-labelledRicinus communis agglutinin to detect lectin-binding sites of the pancreatic B-cell surface, we show that limited regions of the plasma membrane are deprived of lectin-binding sites over marginated secretory granules. Such deprived regions increased during glucose stimulation of B-cells in monolayer culture: 56±8 of them were found in high (300 mg/100 ml) glucose as compared to only 27±5 in low (50 mg/100ml) glucose (p〈0.005). In addition, non-membrane, intracytoplasmic bridges were detected between the plasma membrane and the membrane of the marginated granule suggesting the involvement of cell web components in promoting the change in surface labelling.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01225464
