ISSN:
1432-1106
Keywords:
Cerebellum
;
Purkinje cell
;
Spine
;
Freeze-fracture
;
Membrane
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The number and freeze-fracture membrane organization of spines on Purkinje cell dendrites were studied in rats after destruction, by intraperitoneal injection of 3-acetyl-pyridine (3-AP), of the climbing fiber afferents to the cerebellum. The results obtained show that: (1) there is a 1.6 fold increase in the total number of dendritic spines in the 3-AP-treated animals as compared to controls, but no change in the total number of spine synaptic profiles; (2) the spines from main dendrites in both control and 3-AP-treated animals have more than 1,000 IMP/μm2 of P-face membrane; and, (3) the spines formed on Purkinje cell dendrites in the absence of their presynaptic axons have the same P-face membrane organization (i.e. more than 1,000 IMP/μm2) as spines normally innervated by afferent climbing fibers. These data suggest that each type of Purkinje cell spine has a specific P-face membrane organization which does not appear to be influenced by presynaptic terminals.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00237221
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