ISSN:
1432-2013
Keywords:
Lacrimal gland
;
Cl− activity
;
Acetylcholine
;
Cl− permeability
;
Ca2+ ionophore
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Using double-barreled Cl−-sensitive microelectrodes, intracellular Cl− activity (A Cl i ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA Cl i was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (V m) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedV m to −63 mV and decreasedA Cl i to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inV m andA Cl i to those induced by ACh. It was concluded that Cl− was actively accumulated in the acinar cells and, in the secretory phase, Cl− efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl− permeability across the cell membrane.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00584530
