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HLA-DR genotyping by restriction fragment length polymorphism analyses (1985)

Hui, K. ; Festenstein, H. ; Klein, A. ; [et al.]

Springer

Immunogenetics 22 (1985), S. 231-239

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ISSN: 1432-1211

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract We have established unique restriction fragment length polymorphism (RFLP) patterns characteristic of homozygous typing cells (HTCs) for HLA-DR-1 through HLA-DR-8 haplotypes. These RFLP patterns were found to segregate in family members and correlate 100% with HLA-DR antibody phenotyping. The RFLP patterns were used to type chronic myelocytic leukemic cells which have a Philadelphia translocation from 23 randomly selected Caucasoid patients. The results show an alternative method for the determination of the HLA-DR types without using live cells and to study disease association with the HLA-DR region.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00404482

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