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  • Articles: DFG German National Licenses  (17)
  • 1985-1989  (11)
  • 1980-1984  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Epstein-Barr Virus-induced Lymphoblastoid Cell Lines from Patients with Primary Immunodeficiency Diseases (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 11 (1980), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Peripheral lymphocytes from eight patients with congenital immunodeficiency diseases were infected with Epstein-Barr virus (EBV) in an attempt to establish B lymphoblastoid cell lines (LCL). These patients included three boys with congenital agammaglobulinaemia, two girls with hypogammaglobulinaemia, one boy with common variable immunodeficiency, one boy with severe combined immunodeficiency with adenosine deaminase deficiency, and one boy with DiGeorge syndrome. Five of the patients bore no surface immunoglobulins (slg) on their peripheral lymphocytes. LCL were established from seven of the eight patients. All the LCL established formed rosettes with EAC3 and had the ability to produce cytoplasmic immunoglobulins (clg) of various classes. Culture supernatants concentrated up to 100-fold developed precipitin bands by Ouchterlony's method with antisera to human Ig in all the established LCL. These results suggested that both slg-, clg- and C3+ cells and slg+, clg- and C3+ cells might be the target cells for EBV and that slg-, clg- and C3+ cells might be the precursor cells of B lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1980.tb00221.x
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  • 2
    Electronic Resource
    Electronic Resource
    The effects of forsterite film on total loss of grain-oriented 3% silicon iron (1985)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 57 (1985), S. 4214-4216 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Two types of mill glass, one of which is conventional and the other is characterized by its irregular surface morphology and stronger tension, were prepared on the surface of high permeability 3%Si-Fe sheets in order to clarify the effects of the mill glass on core loss and domain structure. The results are twofold. Firstly, the mill glass of the latter type was, by itself, found to reduce the total loss in the induction range of 1.7 T. Secondly, considerable change in magnetization, characterized by its heavily constricted curve occurred, and corresponding change in the domain structure was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.334617
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    d-lactic acidosis in two patients with short bowel syndrome: Bacteriological analyses of the fecal flora (1982)
    Springer
    European journal of pediatrics 138 (1982), S. 324-326 
    Details
    ISSN: 1432-1076
    Keywords: d-lactic acidosis ; Short bowel syndrome ; Lactobacillus ; Kanamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Two cases of d-lactic acidosis associated with short bowel syndrome are described. The administration of kanamycin to the patients showed a decrease in d-lactate in blood and urine in parallel with disappearance of metabolic acidosis. Bacteriological analyses of the fecal flora showed an increase in Lactobacillus buchneri in the first patient and Lactobacillus fermenti IVa in the second; both bacteria were sensitive to kanamycin. Quantification of in vitro production of d-lactate by each species of bacteria isolated from the feces revealed that Lactobacillus produced more d-lactate than other species of bacteria. These observations indicate that Lactobacillus may play an important role in the induction of d-lactic acidosis in patients with short bowel syndrome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442509
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  • 4
    Electronic Resource
    Electronic Resource
    Serum bile acids and their conjugates in breast-fed infants with prolonged jaundice (1985)
    Springer
    European journal of pediatrics 144 (1985), S. 37-40 
    Details
    ISSN: 1432-1076
    Keywords: Serum bile acid ; Breast milk jaundice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Serum bile acids and their conjugates were analysed in 20 breast-fed infants with prolonged jaundice. The mean total bile acid levels in serum were increased in the breast-fed infants with jaundice, as compared with those in either breastor bottle-fed infants without jaundice. However, there were no significant differences between the groups. All the breast-fed infants examined, regardless of association with jaundice, had a bile acid pattern dominated by taurine conjugates (the ratio of glycine- to taurine-conjugated bile acid, G/T ratio, less than 1.00). In contrast, the bottle-fed infants without jaundice had a pattern dominated by glycine conjugates (G/T ratio, more than 1.00). Among the breast-fed infants with jaundice, the mean G/T ratio in those who had serum bilirubin levels over 10 mg/100 ml was significantly lower than that in those who had serum bilirubin levels of less than 10 mg/100 ml. The altered bile acid metabolism might be associated with the pathology of breast milk jaundice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491922
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Significance of serum lipoprotein-X and gammaglutamyltranspeptidase in the diagnosis of biliary atresia. A preliminary study in 27 cholestatic young infants (1986)
    Springer
    European journal of pediatrics 145 (1986), S. 54-57 
    Details
    ISSN: 1432-1076
    Keywords: Lipoprotein-X ; γ-Glutamyltranspeptidase ; Biliary atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract As simple and nonsurgical means of differentiating biliary atresia (BA) from intrahepatic cholestasis of unknown origin (IC), liver function tests including serum lipoprotein-X (LP-X) and γ-glutamyltranspeptidase (GGTP) were done and evaluated for their usefulness in the diagnosis of 27 cholestatic Japanese young infants. Except for LP-X and GGTP levels (P〈0.01, P〈0.001), there were no significant differences between the BA (n=11) and IC (n=13) groups. When values of mean plus 4 standard deviations were used to differentiate BA from IC (89 mg/100 ml for LP-X and 194 IU/l for GGTP), all BA patients gave positive results for either the crtical LP-X of GGTP values. On the other hand, all IC patients gave negative results for both levels, although patients with a paucity of intrahepatic biliary ducts (n=3) were also positive for either the critical LP-X or GGTP values. The combination test with serum LP-X and GGTP is recommended for helping to differntiate BA from IC in cholestatic young infants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441853
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Successful transplantation of soy bean agglutinin-fractionated, histoincompatible, maternal marrow in a patient with severe combined immunodeficiency and BCG infection (1985)
    Springer
    European journal of pediatrics 143 (1985), S. 291-294 
    Details
    ISSN: 1432-1076
    Keywords: Severe combined immunodeficiency ; HLA-haplotype mismatched bone marrow transplantation ; Soy bean agglutinin ; BCG infection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A successful transplantation of soybean agglutinin (SBA) and sheep red blood cell (SRBC)-fractionated, maternal marrow in a patient with severe combined immunodeficiency (SCID) is reported. The engraftment of HLA-haplotype mismatched marrow cells was obtained without apparent graft versus host disease (GVHD). With immunological reconstituion the patient recovered from a BCG infection, which might have been caused by a BCG inoculation before his bone marrow transplantation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442303
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  • 7
    Electronic Resource
    Electronic Resource
    Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells (1981)
    Springer
    Archives of virology 70 (1981), S. 33-41 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Morphogenesis of human rotavirus type 2 Wa strain in MA 104 cells was observed. The virus antigen in the cytoplasm was detected by indirect immunofluorescence twelve hours after infection. The cytopathic effect occurred 24 hours after infection when virus particles were detected by EM in the culture fluid as well as in thin sections of the infected cells. Virus particles were observed in the dilated RER, nuclear envelope (perinuclear space), viroplasm, and a lysosome-like body. Three types of virus particles were noted: double-shelled particles 75–85 nm in diameter, single-shelled particles 64–68 nm in diameter and electron-dense nucleoids or cores 32–40 nm in diameter. The outer shell of virus particles was acquired by budding through the membrane of the dilated RER. Tubular structures, similar in diameter to the single-shelled particles, were found in the cytoplasm and nucleus of infected MA 104 cells. Bundles of filaments or the leaflet-like inclusion bodies of membrane-bounded bundles of filaments were found in the cytoplasm and seemed to be associated with virus particles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01320791
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Evidence for endocytosis-independent infection by human rotavirus (1987)
    Springer
    Archives of virology 97 (1987), S. 93-99 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of five lysosomotropic drugs (NH4Cl, chloroquine, methylamine, amantadine and dansylcadaverine) and cytochalasin B on human rotavirus (HRV KUN strain) and vesicular stomatitis virus (VSV Indiana strain) infection in monkey MA 104 cells were examined. These drugs had little effect on HRV yield but greatly reduced VSV yield. The results strongly suggest that HRV does not require endocytotic activity and intracellular acidic vesicles for the initial stage of infection and support our postulate that HRV enters the target cell by direct penetration of its nucleoid through the cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310737
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Further observations on the morphogenesis of human rotavirus in MA 104 cells (1984)
    Springer
    Archives of virology 79 (1984), S. 147-159 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human rotavirus “KUN” strain was cultivated in a fetal rhesus monkey kidney cell line, MA 104 cells. Four types of virus particles in cells infected with KUN strain were clearly identified: nucleoid cores, single-shelled particles, double-shelled particles, and membrane band, “enveloped” particles. “Enveloped” particles were found only in the thin sections of infected cells. When first visible, the virus precursors appeared at the ribosome free membrane of rough endoplasmic reticulum (RER), increasing in size while simultaneously being coated with nucleocapsid, inner shell. Single-shelled particles were also synthesized within bundles of filaments of viroplasm in the cytoplasma. During subsequent virus maturation two types of “budding” processes were observed. Double-shelled particles arising at the RER membrane entered the cisternae of the RER through an exocytosis-like process. In contrast, the “enveloped” particles developed in the cisternae by being completely enclosed with RER membrane, and later during cytolysis released the single-shelled particles. These “enveloped” virus particles appeared to be the result of inefficient virus maturation at the last stage of outer capsid formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310808
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  • 10
    Electronic Resource
    Electronic Resource
    Patterns of polypeptide synthesis in human rotavirus infected cells (1986)
    Springer
    Archives of virology 90 (1986), S. 29-40 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Polypeptide analysis of three strains of human rotavirus (KUN, Wa and MO) were conducted using a hypertonic culture which suppressed host protein synthesis and unmasked rotavirus specific protein synthesis. As a result, eleven human rotavirus specific polypeptides (Vp 1–Vp 11) were detected by pulselabeling infected cells with [14C]-leucine. Among the 11 polypeptides, three polypeptides (Vp 7, Vp 10 and Vp 11) underwent post-translational processing, and two (Vp 7 and Vp 10) were glycosylated. Six polypeptides (Vp 1, 2, 3, 4, 6 and 7) were identified as viral structural proteins. Comparisons of three strains of different serotypes revealed that their polypeptide profiles differed from each other in electrophoretic mobility; in particular, profiles of the glycosylated polypeptide, Vp 7, were distinct among the three strains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314142
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