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  • Articles: DFG German National Licenses  (2)
  • Diffusionskammertechnik  (1)
  • Gonadal toxicity  (1)
  • 1
    ISSN: 1432-1440
    Keywords: Germ cell tumors ; Gonadal toxicity ; Chemotherapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The impact of aggressive chemotherapy on reproductive and endocrine gonadal function was prospectively studied in 44 patients with germ cell tumors. Diagnostic procedures to determine gonadal toxicity consisted of hormone determinations, semen analyses, interviews with a standardized questionnaire, and gonadal histology. After chemotherapy all patients showed elevated serum levels of follicle-stimulating hormone (FSH) and azoospermia due to germ cell and stem cell loss. Recovery of spermatogenesis, as indicated by normalization of serum FSH levels and sperm density, occurred in 77% of the patients 25–60 months after cessation of chemotherapy. In all patients serum testosterone and luteinizing hormone (LH) values remained within normal limits after therapy indicating resistance of Leydig cells to cytotoxic drugs. Three patients fathered four healthy children after completion of chemotherapy. These data suggest significant reproductive dysfunction in all men treated for germ cell tumors. However, most patients showed late and complete recovery of spermatogenesis. In contrast, endocrine gonadal function was unaffected after chemotherapy in all patients. FSH and LH are feasible markers to assess drug-induced gonadal toxicity.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 38 (1979), S. 383-389 
    ISSN: 1432-0584
    Keywords: Diffusionskammertechnik ; periphere Blutzellen ; Normalwerte ; Diffusion chambers ; Peripheral blood cells ; Normal values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Growth of mononuclear cells from human peripheral blood from 10 normal individuals was tested in diffusion chamber culture over a period up to 17 or 21 days. After an initial decrease during the first few days an increase of the total cell number was observed with maximal values on day 13. In all individuals growth of undifferentiated blast cells, lymphocytes, plasma cells, immature and mature granulopoietic cells, macrophages, and megakaryocytes occurred. In all individuals the different cell types had similar growth patterns in diffusion chamber culture. The considerable numerical variations which were seen in the granulopoietic cells were probably due to different stem cell concentrations in the peripheral blood of the investigated individuals. The results indicate that the diffusion chamber technique is a valuable method for the detection of haemopoietic stem cells and the culture of lymphocytic cells in man.
    Notes: Zusammenfassung Das Wachstum mononukleärer Zellen des peripheren Blutes in der Diffusionskammerkultur wurde bei 10 Normalpersonen über einen Zeitraum von 17 bis 21 Tagen untersucht. Während der Kultur kam es nach einem initialen Abfall zu einem Anstieg der Gesamtzellzahl mit einem Maximum am 13. Tag. Bei allen untersuchten Individuen zeigte sich eine gute übereinstimmung in den Wachstumsmustern der einzelnen Zellarten. Erhebliche numerische Schwankungen wurden jedoch bei den granulopoetischen Zellen beobachtet, wo sie auf unterschiedliche Stammzellkonzentrationen im Blut der untersuchten Personen zurückgeführt werden müssen. Die Ergebnisse zeigen, da\ die Diffusionskammertechnik eine wertvolle Methode zum Nachweis hämopoetischer Stammzellen und zur Kultur lymphatischer Zellen beim Menschen darstellt.
    Type of Medium: Electronic Resource
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