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  • 21
    Electronic Resource
    Electronic Resource
    Gas–Liquid Chromatographic Separation of Sulphur Compounds (1964)
    [s.l.] : Nature Publishing Group
    Nature 201 (1964), S. 817-818 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We have found that gas?liquid chromatography is applicable to the separation of sulphide?sulphoxide?sulphone mixtures. Further, by using an internal hydrocarbon standard almost positive identification of the sulphur derivative can be obtained. If weight response factors between each sulphur ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/201817a0
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  • 22
    Electronic Resource
    Electronic Resource
    Glycation of rat sciatic nerve tubulin in experimental diabetes mellitus (1991)
    Springer
    Diabetologia 34 (1991), S. 387-389 
    Details
    ISSN: 1432-0428
    Keywords: Diabetes ; glycation ; tubulin ; microtubule ; sciatic nerve ; brain ; axonal transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Diabetic neuropathy is associated with some early defects of axonal transport in experimental animals. Axonal transport is dependent on intact microtubules, and unsubstituted lysine residues of tubulin are essential for microtubule polymerization. As lysine residues are the major target for the non-enzymatic attachment of glucose, the effect of diabetes on the extent of glycation of tubulin was investigated. There was a more than four-fold increase in the extent of glycation of tubulin in the sciatic nerve of rats with streptozotocin-induced diabetes of 2 weeks duration compared with control rats. In contrast, no such increase in glycation was observed in brain microtubule protein from diabetic rats at that stage of diabetes. Incubation of brain microtubule protein with glucose prior to in vitro polymerization showed that the early stages of glycation were not associated with inhibition of microtubule assembly. The observed glycation of peripheral nerve tubulin in early experimental diabetes may nevertheless contribute to axonal transport abnormalities through an as yet undetermined impairment of microtubule function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403175
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  • 23
    Electronic Resource
    Electronic Resource
    Inheritance of mitochondrial DNA in lentil (Lens culinaris Medik.) (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 206-210 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Mitochondrial DNA ; Maternal inheritance ; Biparental mitochondrial inheritance ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. culinaris ‘ILL5588’, and one restriction fragment polymorphism distinguished L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. orientalis ‘LO4’. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an ‘Eston’ x ‘ILL5588’ cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225143
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  • 24
    Electronic Resource
    Electronic Resource
    Paternal plastid DNA can be inherited in lentil (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 607-610 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Chloroplast DNA Maternal plastid inheritance ; Biparental plastid inheritance ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment analysis was used to study the inheritance of chloroplast DNA (cpDNA) in F1 progeny from crosses between Lens culinaris ssp. orientalis and L. culinaris ssp. culinaris. Twenty-five combinations of 11 restriction enzymes and three heterologous probes from Petunia hybrida cpDNA were used to screen six accessions of L.c. culinaris and one accession of L. c. orientalis for restriction fragment length polymorphisms (RFLPs). No variation in cpDNA was observed within the subspecies L. c. culinaris, but the L. c. orientalis accession was unambiguously distinguished from all six L. c. culinaris accessions by two RFLPs. Of ten F1 progeny from L. c. orientalis x L. c. culinaris crosses, nine had only maternal cpDNA restriction fragments but one F1 plant inherited cpDNA fragments from both parents. Nuclear DNA inheritance was biparental in all ten F1 progeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222122
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  • 25
    Electronic Resource
    Electronic Resource
    Changes in specific radioactivities of corn-leaf metabolites during photosynthesis in 14CO2 and 12CO2 at normal and low oxygen (1974)
    Springer
    Planta 120 (1974), S. 113-123 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The 12CO2- and 14CO2-exchange of illuminated corn leaf discs were measured at normal (21%) and low (1%) oxygen. After periods of exposure to 14CO2 or to 14CO2 followed by 12CO2, the discs were killed and the specific activities of some metabolites were determined. At both O2 concentrations the specific activity of 3-PGA increased and decreased rapidly during the first 5 min of 14CO2-feeding or 12CO2-flushing but did not equilibrate with that of the CO2 in the assimilation chamber even after 15 min. The specific activity of aspartic acid also showed bimodal kinetics during both feeding and flushing. The specific activities of 3-phosphoglyceric acid (3-PGA), aspartic acid and alanine were higher at 1% O2 than at 21% O2, but glycine and serine were lower in specific activity at 1% O2. The results are in agreement with the proposed initial fixation of CO2 into C4-dicarboxylic acids and subsequent transfer of this carbon to 3-PGA. Indirect evidence supports the idea that at 21% O2, CO2 was produced by the corn leaf discs in the light and was refixed into C4-dicarboxylic acids. At 1% O2, the photorespiratory process could also have been active although the flux of carbon through the glycolate pathway was probably smaller than at 21% O2.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384921
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  • 26
    Electronic Resource
    Electronic Resource
    Non-destructive semi-quantitative determination of acetylene reduction in the field (1980)
    Springer
    Plant and soil 56 (1980), S. 335-340 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Field method ; Nitrogen fixation ; Non-destructive ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Direct injection of acetylene into soil around plant roots, followed by determination of ethylene/acetylene ratios in the soil atmosphere has been tested as a rapid, non-destructive method of estimating acetylene reducing activity. In pots of artificial media as well as in field soil, the ratios determined within 10 min. after injection were significantly correlated with the rates of acetylenedependent ethylene production in detached roots. The method may be useful in preliminary screening of large numbers of plant-bacteria combinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02205862
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  • 27
    Electronic Resource
    Electronic Resource
    Über den Nachweis von Verfälschungen der Butter mit pflanzlichen Ölen (1955)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 147 (1955), S. 284-284 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01858073
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  • 28
    Electronic Resource
    Electronic Resource
    Ion separation on a thread (1960)
    Springer
    Microchimica acta 48 (1960), S. 831-835 
    Details
    ISSN: 1436-5073
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Vorläufige Ergebnisse zeigen, daß die Möglichkeit besteht, die Empfindlichkeit der Kationentrennung durch stufenweise Extraktion zu steigern, indem man die Extrakte auf einem Nähfaden konzentriert. Chromatographische Trennung auf dem Faden kann dazu benutzt werden, den Nachweis der einzelnen Metalle zu vereinfachen.
    Abstract: Résumé Des essais préliminaires indiquent que la sensibilité de la séparation par extraction avec un solvant peut être améliorée en concentrant les extraits sur un fil. La séparation chromatographique peut être ajoutée pour simplifier l'identification des métaux.
    Notes: Summary Preliminary trials indicate that the sensitivity of separation by solvent extraction may be improved by concentrating the extracts upon a thread. Chromatographic separation may be added to simplify the identification of the metals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01216072
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