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  • Electronic Resource  (8)
  • 1990-1994  (3)
  • 1980-1984  (5)
  • Immunohistochemistry  (4)
  • Acclimation  (1)
  • Arrector pili muscle  (1)
  • Astropecten latespinosus  (1)
  • Bloodstains  (1)
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Material
  • Electronic Resource  (8)
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Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Occurrence of tetrodotoxin in the starfishAstropecten latespinosus (1984)
    Springer
    Cellular and molecular life sciences 40 (1984), S. 1395-1396 
    Details
    ISSN: 1420-9071
    Keywords: Astropecten latespinosus ; starfish ; tetrodotoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A toxin causing paralysis was detected in the starfishAstropecten latespinosus. The toxin was purified by a method consisting of charcoal treatment and chromatography on CM-Sephadex C-25 and Bio-Rex 70. The toxin was identified as tetrodotoxin by its behavior in thin-layer chromatography and electrophoresis and its1H-NMR spectrum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01951908
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  • 2
    Electronic Resource
    Electronic Resource
    Polymorphism of coagulation factor XIII B subunit: Further occurrence of FXIIIB*15 in Japanese and phenotyping in bloodstains (1992)
    Springer
    International journal of legal medicine 104 (1992), S. 317-319 
    Details
    ISSN: 1437-1596
    Keywords: Polymorphism FXIIIB ; Population genetics ; Bloodstains ; Polymorphismus FXIIIB ; Populationsgenetik ; Blutspuren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Der Polymorphismus des FXIIIB wurde bei 555 unverwandten Japanern mit Hilfe der isoelektrischen Fokussierung und anschließendem Immunoblotting untersucht. Fünf allgemein vorkommende Phänotypen und die seltene Variante FXIIIB 15-3 wurden beobachtet. Die Allel-Frequenzen waren FXIIIB = 0,3063, FXIIIB2 = 0,0162, FXIIIB3 = 0,6766 und FXIIIB15 = 0,0009. Die Bestimmung der Phänotypen war auch an Blutspuren mit folgenden Lagerungsbedingungen möglich: Bei 37°C über einen Zeitraum bis zu 4 Monate, bei Raumtemperatur und bei 4°C länger als 6 Monate. Das FXIIIB-System kann einen neuen, aussagekräftigen genetischen Marker für gerichtsmedizinische Blutspurenuntersuchungen darstellen.
    Notes: Summary The polymorphism of FXIIIB was investigated in 555 unrelated Japanese individuals using isoelectric focusing and immunoblotting. Five common phenotypes and a rare variant type FXIIIB 15-3 were observed. The allele frequencies were FXIIIB*1 = 0.3063, FXIIIB*2 = 0.0162, FXIIIB*3 = 0.6766 and FXIIIB*15 = 0.0009. Phenotyping was also possible from bloodstains stored at 37°C for up to 4 months and from bloodstains stored at room temperature and at 4°C for over 6 months. The FXIIIB system can provide a new powerful genetic marker for the medicolegal grouping of bloodstains.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369549
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemical demonstration of the localization of corticotropin releasing factor-containing neurons in the hypothalamus of mammals including primates (1982)
    Springer
    Anatomy and embryology 165 (1982), S. 303-313 
    Details
    ISSN: 1432-0568
    Keywords: CRF neurons ; Hypothalamus ; Immunohistochemistry ; PAP ; Mammals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence of the CRF-containing neurons in the hypothalamus was investigated in four different species (cats, dogs, pigs, and monkeys) by the peroxidase-antiperoxidase technique using specific anti-serum to CRF. In all animals examined, CRF-containing perikarya were found mainly in the paraventricular and supraoptic nuclei, and a small number of the immunoreactive cells were observed in the accessory supraoptic nucleus and the lateral hypothalamic area. The size of the CRF-containing perikarya ranged from 20–35 μm in diameter. These findings suggest that the magnocellular paraventricular and supraoptic nuclei are the center not only of the classical neurosecretory system for the production of the posterior lobe hormones, but also that of the CRF neuronal system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305569
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  • 4
    Electronic Resource
    Electronic Resource
    A high concentration of Merkel cells in the bulge prior to the attachment of the arrector pili muscle and the formation of the perifollicular nerve plexus in human fetal skin (1993)
    Springer
    Archives of dermatological research 285 (1993), S. 261-268 
    Details
    ISSN: 1432-069X
    Keywords: Merkel cell ; Bulge ; Arrector pili muscle ; Nerve plexus ; Skin development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The distribution of Merkel cells in human fetal hair follicles was studied using whole mounts of separated epidermis with attached hair follicles. The technique had the advantage of enabling the elucidation of the spatial relationships of Merkel cells with other cells in the skin. In a 16-week-old fetus the hair anlagen had formed one or two epithelial swellings of variable size. In a 17-week-old fetus sebaceous glands and the bulge of the hair follicle were recognizable and immunoreactive Merkel cells were present in the bulge and surrounding the acrotrichium (intraepidermal follicular canal). In a 20-week-old fetus the sebaceous gland and bulge were well formed and immunoreactive Merkel cells were concentrated in the bulge and infundibulum. In vertical sections of a 20-week-old fetus immunoreactive Merkel cells were also situated in the vicinity of the bulge. Arrector pili muscles were first observable in a 24-week-old fetus being weakly stained with anti-desmin antibody. In a 24-week-old fetus, nerves were also stained within the arrector pili muscles with S-100 protein antibody. In the presumptive arrector pili muscle immunoreactivity for S-100 protein developed before or at the same time as immunoreactivity for desmin. Merkel cells or their products in the bulge may serve as attractants for the growing arrector pili muscle which contain peripheral nerves. Following our report that dermal Merkel cells influence the formation of the dermal nerve plexus, perifollicular Merkel cells near the bulge may also play an inductive and growth-stimulative role for the perifollicular nerve plexus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371594
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  • 5
    Electronic Resource
    Electronic Resource
    CRF-immunoreactive nerve fibers in the circumventricular organs of the monkey, Macaca fuscata (1983)
    Springer
    Cell & tissue research 232 (1983), S. 679-683 
    Details
    ISSN: 1432-0878
    Keywords: CRF-immunoreactive nerve fibers ; Circumventricular organs ; Immunohistochemistry ; Monkey, Macaca fuscata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The occurrence of CRF (corticotropin-releasing factor)-immunoreactive nerve fibers in the circumventricular organs of adult male monkeys, Macaca fuscata, was studied on serially sectioned brains, by means of the peroxidase-antiperoxidase technique in combination with a highly specific and sensitive CRF antiserum. CRF-containing nerve fibers were found in high concentrations in the infundibulum and, in addition, in small numbers in the posterior lobe, organum vasculosum laminae terminalis, subfornical organ, and area postrema; they were missing in the pineal body and the subcommissural organ. The CRF immunoreactive nerve fibers distributed in these organs were located in the proximity of the blood vessels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216438
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  • 6
    Electronic Resource
    Electronic Resource
    Changes in carp myosin ATPase induced by temperature acclimation (1990)
    Springer
    Journal of comparative physiology 160 (1990), S. 233-239 
    Details
    ISSN: 1432-136X
    Keywords: Temperature ; Acclimation ; Myosin ; Myosin heavy chain ; ATPase activity ; Carp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Myosins were isolated from dorsal ordinary muscles of carp acclimated to 10°C and 30°C for a minimum of 5 weeks and examined for their ATPase activities. Ca2+-ATPase activity was different between myosins from cold-and warm-acclimated carp, especially at KCl concentrations ranging from 0.1 to 0.2 M, when measured at pH 7.0. The highest activity was 0.32 μmol Pi·min-1·mg-1 at 0.2 M KCl for cold-acclimated carp and 0.47 μmol Pi·min-1·mg-1 at 0.1 M KCl for warm-acclimated fish. The pH-dependency of Ca2+-ATPase activity at 0.5 M KCl for both carp was, however, similar exhibiting two maxima around 0.3 μmol Pi·min-1·mg-1 at pH 6 and 0.4 μmol Pi·min-1·mg-1 at pH 9. K+(EDTA)-ATPase activity at pH 7.0 neither exhibited differences between both myosins. It increased with increasing KCl concentration showing the highest value of about 0.4 μmol Pi·min-1·mg-1 at 0.6–0.7 M KCl. Actin-activated myosin Mg2+-ATPase activity was markedly different between cold-and warm-acclimated carp. The maximum initial velocity was 0.53 μmol Pi·min-1·mg-1 myosin at pH 7.0 and 0.05 M KCl for cold-acclimated carp, which was 1.6 times as high as that for warm-acclimated carp. These differences were in good agreement with those obtained with myofibrillar Mg2+-ATPase activity between both carp. No differences were, however, observed in myosin affinity to actin. Differences in myosin properties between cold- and warm-acclimated carp were further evidenced by its thermal stability. The inactivation rate constant of myosin Ca2+-ATPase was 25·10-4·s-1 at 30°C and pH 7.0 for cold-acclimated carp, which was about 4 times as high as that for warm-acclimated carp. Light chain composition did not differ between both carp myosins. The differences in a primary structure of the heavy chain subunit was, however, clearly demonstrated between both myosins by peptide mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00302588
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  • 7
    Electronic Resource
    Electronic Resource
    Differences in the distributional pattern of CRF-, oxytocin-, and vasopressin-immunoreactive nerve fibers in the median eminence of the rat (1983)
    Springer
    Cell & tissue research 230 (1983), S. 247-258 
    Details
    ISSN: 1432-0878
    Keywords: CRF ; Oxytocin ; Vasopressin ; Immunohistochemistry ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Conspicuous differences in the distributional pattern of nerve fibers containing corticotropin-releasing factor (CRF) or posterior lobe hormones, respectively, were shown in the median eminence of the adult male rat by means of immunoperoxidase histochemistry, with the use of anti-CRF, anti-oxytocin, and anti-vasopressin sera. In the rostral and central divisions of the median eminence, a high concentration of CRF-immunoreactive nerve fibers was found in the median portion of the external layer; these fibers terminated on the capillary loops of the hypophysial portal system. In the caudal division of the median eminence, the CRF-immunoreactive nerve fibers were located in the median to paramedian portions of the external layer. Numerous oxytocin- and vasopressin-immunoreactive nerve fibers were observed evenly distributed throughout the internal layer of the median eminence. In the external layer, a small number of the oxytocin- and vasopressin-containing nerve fibers was found around the capillary loops, particularly in the median to paramedian portions. The distributional patterns of the CRF and the posterior lobe hormones in the hypothalamo-hypophysial system and their functional interrelationship are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213803
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  • 8
    Electronic Resource
    Electronic Resource
    Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus (1983)
    Springer
    Cell & tissue research 230 (1983), S. 239-246 
    Details
    ISSN: 1432-0878
    Keywords: CRF neurons ; Hypothalamus ; Immunohistochemistry ; PAP ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A specific rabbit anti-CRF serum and the immunoperoxidase technique were used to show that CRF-containing neurons are mainly distributed in the paraventricular and supraoptic nuclei of the rat hypothalamus. In addition, immunoreactive neurons are scattered in other hypothalamic regions. These neurons are 20–30 μm in diameter. From the present and previous investigations it may be concluded that the hypothalamic magnocellular nuclei, i.e., paraventricular and supraoptic, and other hypothalamic accessory nuclei, are the producing sites not only for vasopressin and oxytocin, but also for corticotropin-releasing factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00213802
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