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  • 11
    Electronic Resource
    Electronic Resource
    Cerebral metabolic changes in delayed carbon monoxide sequelae studied by proton MR spectroscopy (1994)
    Springer
    Neuroradiology 36 (1994), S. 104-106 
    Details
    ISSN: 1432-1920
    Keywords: Carbon monoxide poisoning ; Delayed carbon monoxide sequelae ; Proton magnetic resonance spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The pathogenesis of delayed sequelae of carbon monoxide (CO) exposure is still unknown. We repeatedly examined a 55-year-old woman with the interval form of CO poisoning, using proton magnetic resonance spectroscopy (MRS). When the clinical picture was severe, MRS revealed markedly lowered N-acetyl-asparatate (NAA)/creatine and phosphocreatine (Cr) ratio and slightly increased choline containing compounds (Cho)/Cr ratio. Subsequently, NAA and Cho/Cr ratio tended to return to normal, reflecting clinical improvement. Proton MRS shows the previously unrecognised neuronal activity in CO poisoning and precisely reflects the severity of symptoms. We stress the superiority of proton MRS over the conventional radiological examinations in CO poisoning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00588070
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  • 12
    Electronic Resource
    Electronic Resource
    Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures (1990)
    Springer
    Archives of virology 114 (1990), S. 293-298 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cytotropism of two strains, GDVII and DA, of Theiler's murine encephalomyelitis viruses (TMEV) was studied in the oligodendrocyte-enriched murine neural cell cultures. Both GDVII and DA caused cytopathic effects in the neural cell cultures, and double immunostaining for galactocerebroside (Gal-Cer), a marker molecule for oligodendrocyte, and viral antigens disclosed a dual expression of Gal-Cer and viral antigens in over 80% of cells in both cultures 24h after infection with either GDVII or DA. The kinetics of cell-free and cell-associated infectivity were not significantly different between two cultures. These in vitro observations suggest that neither replication in oligodendrocyte nor cell-associated infectivity is a sole factor in discriminating those two subgroups of TMEV with regard to the demyelinating activity, and that virus cell binding may play an important role in virus persistence and TMEV-induced demyelination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310760
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  • 13
    Electronic Resource
    Electronic Resource
    Search for human T-cell leukemia virus type I (HTLV-I) proviral sequences by polymerase chain reaction in the central nervous system tissue of HTLV-I-associated myelopathy (1992)
    Springer
    Archives of virology 124 (1992), S. 31-43 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using the polymerase chain reaction (PCR), proviral DNA sequences of thepol andenv regions of human T-cell leukemia virus type I (HTLV-I) were directly amplified in paraffin-embedded and frozen tissue sections of active inflammatory central nervous system (CNS) lesions in three autopsy cases of HTLV-I-associated myelopathy (HAM) with serological confirmation. In parallel, the enumeration of UCHL-1 (monoclonal antibody reactive to T-cells) positive cells in the tissue sections subjected to PCR were carried out. Although the control DNA sequence of parathyroid hormone-like peptide gene was definitely amplified, no signals for HTLV-I proviral sequences were detected in these specimens. The number of UCHL-1 positive cell nuclei was almost on the border line of our PCR sensitivity in formalin-fixed tissue, which was estimated to be 20–200 copies. Therefore, it is unlikely that the central nervous system tissue damage in HAM/TSP is a consequence of productive infection of HTLV-I in the CNS tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01314623
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  • 14
    Electronic Resource
    Electronic Resource
    Ultrastructural study on the sequence of human cytomegalovirus infection in human diploid cells (1973)
    Springer
    Archives of virology 40 (1973), S. 311-324 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The sequence of human CMV infection in WI 38 cells at high input multiplicity was studied by electron microscopy. The majority of entering virus particles were degraded within the cytoplasmic vacuoles but few particles released their capsids into the cytoplasm. Unenveloped particles with a distinct core were observed to persist over a period of 24 hours post-infection. Virus particles in the nucleus were only detectable 48 hours post-infection and thereafter. Process of formation and segregation of primary lysosome-like dense bodies was observed in association with the termination of the virus assembly cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01242551
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  • 15
    Electronic Resource
    Electronic Resource
    Reappraisal of regional thiamine content in the central nervous system of the normal and thiamine-deficient mice (1993)
    Springer
    Metabolic brain disease 8 (1993), S. 45-59 
    Details
    ISSN: 1573-7365
    Keywords: thiamine content ; thiamine deficiency ; HPLC analysis ; Wernicke-Korsakoff syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The regional distribution of thiamine and its phosphate esters was measured in the central nervous system (CNS) of normal and thiamine-deficient mice. Twelve small areas were punched out from frozen sections and they were individually analyzed by high performance liquid chromatography (HPLC). Regional difference was noted in both the content and ratio of thiamine and its phosphate esters in the normal CNS. In pyrithiamine-induced thiamine deficiency, thiamine pyrophosphate (TPP) content in all the areas was reduced to less than 13% of the control values on day 10, when the neurological signs developed. Although there were considerable regional variations in the reduction rate of thiamine and its phosphate esters, no correlation was established between the severity of tissue damage and the magnitude of thiamine reduction in individual areas. These results indicate that a derangement in TPP-dependent processes, either alone or in combination with other factors, plays a more critical role in the neuronal damage under thiamine deficiency than depletion of thiamine compoundsper se.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01000529
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  • 16
    Electronic Resource
    Electronic Resource
    Estimation of distribution of vinyl groups in micron-sized monodisperse polymer microspheres by bromine titration method (1991)
    Springer
    Colloid & polymer science 269 (1991), S. 1126-1132 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; microsphere ; vinylgroup ; brominetitration ; dispersionpolymerization ; seededpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The distribution of vinyl groups in polystyrene/poly(styrene-divinylbenzene) (PS/P(S-DVB)) composite microspheres produced by seeded copolymerization of S and DVB in the presence of 2.1-μm monodisperse PS seed particles was estimated from the amount detected with bromine titration method as a function of the reaction time under various conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00654120
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  • 17
    Electronic Resource
    Electronic Resource
    Interpretation of COHb concentrations in the left and right heart blood of cadavers (1992)
    Springer
    International journal of legal medicine 105 (1992), S. 65-68 
    Details
    ISSN: 1437-1596
    Keywords: Fire victim ; Exposure before or after death ; Carbon monoxide hemoglobin ; Gas chromatography ; Brandopfer ; Antemortale und postmortale Exposition ; Kohlenmonoxid-Hämoglobin ; Gaschromatographie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe der Gaschromatographie wurde die Kohlenmonoxid-Konzentration im Blut des rechten und linken Herzens vergleichend untersucht. Das Material stammt von Leichen mit antemortaler oder nicht-antemortaler Feuer- oder CO-Exposition. Das Konzentrationsverhältnis von COHb zwischen linkem und rechtem Herzblut (L/R-Quotient) scheint ein nicht brauchbares Kriterium zu sein, um festzustellen, ob der Tod sich vor oder nach der Feuer-Exposition ereignet hat; mit der Ausnahme von solchen Fällen, wo bei makrokopischer Untersuchung kein Ruß in den Atemwegen gefunden wird und wo die COHb-Konzentration innerhalb jener Grenzen ist, welche für Zigaretten-Raucher als normal angesehen werden.
    Notes: Summary Carbon monoxide hemoglobin (COHb) concentrations in left and right heart blood samples from cadavers both exposed and not exposed to fire or CO gas were analyzed by the gas chromatographic method. The COHb concentration ratio between samples of left and right heart blood (L/R ratio) does not appear to be useful for establishing whether death has occurred before or after exposure to fire with the exception of cases where no soot can be detected in the airways by the naked eye and the COHb concentration in the blood sample is within the level considered normal for tobacco smokers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02340825
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  • 18
    Electronic Resource
    Electronic Resource
    Preparation of micron-size monodisperse polymer microspheres having cationic groups (1992)
    Springer
    Colloid & polymer science 270 (1992), S. 733-737 
    Details
    ISSN: 1435-1536
    Keywords: Polymeremulsion ; microsphere ; chloromethylgroup ; polyamine ; cationicgroup ; dispersionpolymerization ; seededpolymerization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Micron-size monodisperse crosslinked polymer microspheres having chloromethyl groups were prepared by seeded copolymerization of styrene, divinylbenzene, and chloromethylstyrene in the presence of 2.1-μ monodisperse polystyrene seed particles produced by dispersion polymerization. The modification reaction of chloromethyl groups on the surfaces with polyamines such as triethylenetetramine and ethylenediamine was carried out. From the measurements of ξ potential and the amount of chloride ion released, the introduction of a large number of cationic groups at the surfaces was confirmed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00776143
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