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  • 1
    Electronic Resource
    Electronic Resource
    Alpha-fetoprotein-producing capacity, chromosomal and morphological properties in human hepatoma cells treated with various chemotherapeutic agents (1989)
    Springer
    Research in experimental medicine 189 (1989), S. 409-419 
    Details
    ISSN: 1433-8580
    Keywords: AFP ; Hepatoma ; Human cell line ; Antineoplastic agents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Alpha-fetoprotein (AFP)-producing capacity and some other properties of human hepatoma cell lines treated with chemotherapeutic agents, such as mitomycin C, Adriamycin, cisplatinum, and 5-fluorouracil were investigated. In the case of hepatoma cells that can be grown in culture following treatment with chemotherapeutic agents, their AFP-producing capacity was almost equal to that of untreated control cells with a few exceptions. On the other hand, in the case of similarly treated hepatoma cells that cannot be grown in culture, their AFP-producing capacity was quite different from that of untreated control cells. Under these conditions chromosomal and morphological aberrations were also observed in the treated cells. The present study shows that AFP-producing capacity of hepatoma cells can be changed by chemotherapeutic agents, probably through chromosomal mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01855008
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of various barbituric acid derivatives on survival of functional hepatocytes from adult rats in primary culture (1987)
    Springer
    Research in experimental medicine 187 (1987), S. 105-117 
    Details
    ISSN: 1433-8580
    Keywords: Primary culture of adult rat hepatocytes ; Barbituric acid derivatives ; Hepatocyte survival ; Albumin secretion ; Tyrosine aminotransferase activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Eighteen barbituric acid (BA) derivatives and three structurally related chemicals (non-BA-derivatives) were tested for their potency in supporting survival of functional hepatocytes from adult rats in primary culture. Of the 18 BA derivatives, nine drugs showed excellent maintenance effect on hepatocyte survival and function. Although four BA derivatives were also effective, their potency was relatively lower. The remaining five BA derivatives and three structurally related chemicals exhibited no maintenance effect. Thus, a correlation was found between the BA derivative structure and the potency for supporting hepatocyte survival in primary culture. The dose response curves of hepatocyte survival were generally biphasic in shape, as a function of BA derivative concentration. The optimum concentrations for observing the morphological and biochemical effects of the BA derivatives differed from each other. The maintenance of hepatocytes was attained only in the continuous presence of the BA derivatives in the medium. The nine excellent BA derivatives efficiently prevented hepatocytes from morphological degeneration which was observed in the control cultures. The surviving hepatocytes in the presence of these BA derivatives showed higher albumin secretion and retained higher basal levels of tyrosine aminotransferase (TAT) activity for at least 2 weeks in primary culture, as compared with control. Furthermore, the addition of dexamethasone (10 µM) caused a 2- to 4-fold induction of TAT activity for at least 2 weeks in primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851972
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  • 3
    Electronic Resource
    Electronic Resource
    Establishment and characterization of a cell line from a human cholangiocellular carcinoma (1988)
    Springer
    Research in experimental medicine 188 (1988), S. 367-375 
    Details
    ISSN: 1433-8580
    Keywords: Cell line ; Human (HuH-28) cells ; Cholangiocellular carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A cell line, HuH-28, was established in vitro from a patient with cholangiocellular carcinoma (CCC). This cell line has grown slowly, revealing a doubling time of approximately 80 h, and the serial passages were carried out 20 times within 10 months. Light microscopy revealed spindle and polygonal morphology of the cells. Chromosome number of the cells were distributed near the hypotriploid region at passages 3 and 14. HuH-28 cells were not transplantable into nude mice, but secreted some tumor markers including alkaline phosphatase (ALP), gamma glutamyltranspeptidase (GGT), β2-microglobulin (BMG), ferritin, elastase-1, and tissue polypeptide antigen (TPA). This HuH-28 cell line will represent a good model for the investigation of carcinogenesis, histogenes, and diagnosis of CCC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851205
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  • 4
    Electronic Resource
    Electronic Resource
    The effect of conditioned medium on fetal human liver cells in primary culture (1986)
    Springer
    Research in experimental medicine 186 (1986), S. 463-468 
    Details
    ISSN: 1433-8580
    Keywords: Conditioned medium ; Colony ; Human liver ; Primary ; Albumin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A medium conditioned by rat embryo cultures (RCM) promoted the adhesion of liver cells from human fetuses to plastic dish. Colonies were also formed in primary cultures of the same cells in the presence of RCM. The majority of the colonies formed were composed of large polygonal cells with a few colonies composed of both clear epithelial-like cells and fibroblast-like cells. RCM was superior to the rat embryo feeder layer for promotion of colony formation of cells. A number of colonies were formed from fetal human livers when a conditioned medium from human hepatoma cells (HCM) was used, but most of the colonies formed were composed of fibroblast-like cells. The cells derived from the polygonal cell colonies, which were formed in the presence of RCM, have been passaged four times and they are still growing with albumin-producing capacity. The effect of RCM was reduced by various physico-chemical treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852200
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  • 5
    Electronic Resource
    Electronic Resource
    Perfusion technique of suckling rat liver, and comparison of cytologic and biochemical properties between hepatocytes isolated from suckling and adult rats (1986)
    Springer
    Research in experimental medicine 186 (1986), S. 121-131 
    Details
    ISSN: 1433-8580
    Keywords: Suckling rat hepatocytes ; Adult rat hepatocytes ; Collagenaseliver-perfusion technique ; Primary culture ; Liver-specific functions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Collagenase-liver-perfusion technique, currently used with adult rat liver, was applied to isolation of hepatocytes from suckling rat liver. The total hepatocyte numbers isolated from suckling rats by collagenase-liverperfusion technique were 9-fold higher than those by non-perfusion technique. The yield and viability of isolated hepatocytes from suckling rats were 18.1 × 107 cells per gram liver and 95%, respectively. The cell yield per gram liver and viability from suckling rats were 185% and 112% of those from adult ones, respectively. In comparison with adult rat hepatocytes, suckling rat hepatocytes were smaller and more homogeneous. The percentage (3.1%) of binucleate cells in the hepatocytes isolated from suckling rats was about one tenth of that (30.7%) in the hepatocytes isolated from adult rats. The isolated suckling rat hepatocytes had higher tyrosine aminotransferase (TAT) and glucose-6-phosphate (G6Pase) activities than adult ones. Suckling and adult rat hepatocytes were transferred to primary culture to compare their cell number kinetics and functional longevities. The functional longevities of those hepatocytes were assessed by their capacity to secrete albumin and alpha-fetoprotein (AFP) into the culture medium and to express TAT and G6Pase activities up to day 6 of primary culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851993
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation of clones from T24 cells and test of their sensitivity to adriamycin (1988)
    Springer
    Research in experimental medicine 188 (1988), S. 239-246 
    Details
    ISSN: 1433-8580
    Keywords: T24 ; Heterogeneity ; Single cell clone ; Adriamycin ; Chemosensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, respectively. CL7 had a higher growth rate than CL3, as indicated by in vitro cell growth, plating efficiency, and growth of the tumors produced in nude mice. CL3 was slightly susceptible to adriamycin toxicity as compared to CL7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852272
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructure of concentric laminations in primary human brain tumors (1986)
    Springer
    Acta neuropathologica 71 (1986), S. 83-87 
    Details
    ISSN: 1432-0533
    Keywords: Astrocytes ; Brain neoplasms ; Oligodendroglioma ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ultrastructural concentric laminations have previously been thought to be specific to oligodendroglioma. However, these structures were also recognized in fibrillary astrocytomas, a mixed glioma and a glioblastoma. These laminations continued or closely related to attenuated processes or cytoplasm of astrocytic tumor cells. In addition, some lamellae contained glial filaments. It is considered that the concentric laminations are derived from attenuated astrocytic processes and have no relationship with myelin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687966
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  • 8
    Electronic Resource
    Electronic Resource
    Capillary hemangioblastoma: histogenesis of stromal cells (1987)
    Springer
    Acta neuropathologica 73 (1987), S. 370-378 
    Details
    ISSN: 1432-0533
    Keywords: Electron microscopy ; Factor VIII ; Glial fibrillary acidic protein ; Hemangioblastoma ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The histogenesis of stromal cells in capillary hemangioblastoma has been the subject of debate. The light and electron microscopic studies of hemangioblastomas presented here showed pericytic and leiomyoblastic features in stromal cells. Cells cultured by the monolayer method showed similar features to those of the original tumors. Immunohistochemical studies for glial fibrillary acidic protein and factor VIII/von Willebrand factor indicated that stromal cells were antigenically distinct from astrocytes and endothelial cells. These findings suggest that stromal cells are closely related to pericytes and smooth muscle cells, and support Rhodin's speculation that pericytes serve as a precursor to smooth muscle cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688262
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  • 9
    Electronic Resource
    Electronic Resource
    Intraoperative ultrasonography through a burr-hole (1985)
    Springer
    Acta neurochirurgica 77 (1985), S. 41-45 
    Details
    ISSN: 0942-0940
    Keywords: Intraoperative ultrasound ; burr-hole ; stereotactic techniques ; echoencephalography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Intraoperative ultrasound diagnosis through a burr-hole was performed in 22 cases using a 5 MHz electronic sector-scanning transducer. The pathology along with the landmarks, such as ventricles and the interhemispheric fissure, were visualized. In two cases, a second burr-hole was placed nearby, and aspiration of the fluid was accomplished under real-time ultrasonic monitoring using the first hole as an acoustic window. Our results suggest a possibility of ultrasound-guided stereotactic surgery of the brain through twin burr-holes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01402304
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  • 10
    Electronic Resource
    Electronic Resource
    2/8 translocation in a Japanese Burkitt's lymphoma (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 742-743 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A new translocation between chromsomes 2 and 8 t(2p−; 8q+), was found in fresh lymphoma cells from a Japanese patient with Epstein-Barr virus-carrying Burkitt's lymphoma, and in a lymphoma cell line derived from this patient. There was no 14q+ translocation, as has been previously described in African and North American Burkitt's lymphomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968217
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