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  • 1
    Electronic Resource
    Electronic Resource
    Secretory tapetum of Brassica oleracea L.: polarity and ultrastructural features (1991)
    Springer
    Sexual plant reproduction 4 (1991), S. 28-35 
    Details
    ISSN: 1432-2145
    Keywords: Tapetal cells ; Brassica oleracea L ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructure of the secretory, binucleate tapetum of Brassica oleracea in the micro spore mother cell (MMC) stage through to the mature pollen stage is reported. The tapetal cells differentiate as highly specialized cells whose development is involved in lipid accumulation in their final stage. They start breaking down just before anther dehiscence. Nuclei with dispersed chromatin, large nucleoli and many ribosomes in the cytoplasm characterize the tapetal cells. The wall-bearing tapetum phase ends at the tetrade stage. The dissolution of tapetal walls begins from the inner tangential wall oriented towards the loculus and proceeds gradually along the radial walls to the outer tangential one. The plasmodesmata transversing the radial walls between tapetal cells persist until the mature microspore, long after loss of the inner tangential wall. After wall dissolution, the tapetal protoplasts retain their integrity and position within the anther locule. The tapetal cell membrane is in direct contact with the exine of the microspores/pollen grains and forms tubular evaginations that increase its surface area and appear to be involved in the translocation of solutes from the tapetal cells to the microspores/ pollen grains. The tapetal cells exhibit a polarity expressed by spatial differentiation in the radial direction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194568
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  • 2
    Electronic Resource
    Electronic Resource
    Esthesioneuroblastoma: Ultrastructural, immunohistological and biochemical investigation of one case (1984)
    Springer
    European archives of oto-rhino-laryngology and head & neck 239 (1984), S. 133-144 
    Details
    ISSN: 1434-4726
    Keywords: Esthesioneuroblastoma ; Intermediate filaments ; Secretory granules ; Ultrastructure ; Immunohistology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of esthesioneuroblastoma, the pathological diagnosis of which almost always causes great difficulties, was investigated ultrastructurally, biochemically, and immunohistologically, using antibodies against the five known types of intermediate filaments [keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and neurofilaments]. The tumour cells did not react with antibodies against any of the five intermediate filament proteins. Ultrastructural investigations showed dense cored secretory granules in the cytoplasm and cell processes. Thus, immunohistology offers by “exclusion” a differential diagnosis to avoid often misdiagnosed tumours (undifferentiated carcinomas, embryonal rhabdomyosarcomas, and malignant lymphomas), since carcinomas react with antikeratin, embryonal rhabdomyosarcomas with antibodies to desmin and malignant lymphomas show immunofluorescence with antibodies to vimentin. The biological behaviour (age distribution, tendency to metastasize), the normal values of biochemical parameters, homovanillic acid and vanilmandelic acid (HVA, VMA), and the absence of neurofilaments distinguish this type of tumour from the peripheral sympathetic neuroblastoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463554
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  • 3
    Electronic Resource
    Electronic Resource
    Meningeal granulocytic sarcoma without evidence of leukemia (1981)
    Springer
    Virchows Archiv 392 (1981), S. 111-118 
    Details
    ISSN: 1432-2307
    Keywords: Granulocytic sarcoma ; Chloroma ; Meningeal sarcoma ; Ultrastructure ; Myelogenous leukemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An unusual case of meningeal granulocytic sarcoma without evidence of Leukemia is presented. The patient, a 40 year old female, presented with a chronic subdural haematoma. Three months later a large meningeal tumor was discovered adjacent to the previous haematoma and was found to be a granulocytic sarcoma by the use of electron microscopy. The tumor was treated by surgical excision followed by radiotherapy and chemotherapy. The patient remains free of symptoms and without evidence of leukemia in the peripheral blood or bone marrow 9 months after the diagnosis was established. The ultrastructural findings in the tumor and diagnostic difficulties often encountered are emphasized.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00430554
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  • 4
    Electronic Resource
    Electronic Resource
    Gastrointestinal autonomic nerve tumours and their separation from other gastrointestinal stromal tumours: an ultrastructural and immunohistochemical study of seven cases (1995)
    Springer
    Virchows Archiv 426 (1995), S. 27-35 
    Details
    ISSN: 1432-2307
    Keywords: Gastrointestinal autonomic nerve tumours ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Gastrointestinal stromal tumours (GIST) represent a heterogeneous group whose classification frequently requires ultrastructural and immunohistochemical studies. In a retrospective study of the ultrastructural findings of 24 gastrointestinal stromal tumours, whose light microscopic study has yielded ambiguous results and in which accurate diagnosis had required ultrastructural support, seven were found to have the characteristics of gastrointestinal autonomic nerve (GAN) tumours. In all of them the diagnosis was based on the presence of dendritic processes with dense neuroendocrine granules. Immunohistochemically, the seven tumours were negative for smooth-muscle markers. All stained positively for vimentin. NSE, chromogranin, and synaptophysin were positive in most of them, while S-100 protein was positive only in two cases. We present the ultrastructural and immunohistochemical features of seven GANT against the background of the GISTs of our series. We conclude that GAN tumours cannot be diagnosed by light microscopy alone but this tumour group displays characteristic electron microscopic and immunohistochemical features and appears to represent a distinct type of GIST.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194695
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  • 5
    Electronic Resource
    Electronic Resource
    Type C retroviral expression in spontaneous feline olfactory neuroblastomas (1990)
    Springer
    Acta neuropathologica 80 (1990), S. 547-553 
    Details
    ISSN: 1432-0533
    Keywords: Feline ; Olfactory neuroblastoma ; Type C retrovirus ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Three cases of spontaneous olfactory neuroblastoma (ONB) in domestic cats were morphologically and immunocytochemically characterized. Diagnostic light microscopic features included Flexner and Homer-Wright rosettes, while ultrastructurally the cells had neuritic processes, intracellular intermediate filaments, and intercellular junctions. Immunocytochemically, the tumors stained positively for neuron-specific enolase, cytokeratins, and S-100 protein antigens. In each case, a key finding was the identification of numerous mature type C retroviral particles within the tumors. In one case, budding of viral particles from the plasmalemma of tumor cells suggested the source of mature particles. This cat and one other were tested, and both were serologically positive for feline leukemia virus (FeLV). The virus in the tumors was identified as FeLV by polymerase chain reaction and immunocytochemistry. No other neoplasms were found in any of the cats, nor was there similar evidence of active viral infection in other non-tumor tissues, including the brain. Although the relationship between FeLV infection and ONB is uncertain, our findings indicate that FeLV should be investigated as an etiologic agent of ONB.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294617
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  • 6
    Electronic Resource
    Electronic Resource
    Ontogenesis of the rabbit pituitary intermediate lobe (1993)
    Springer
    Anatomy and embryology 187 (1993), S. 87-97 
    Details
    ISSN: 1432-0568
    Keywords: Pituitary intermediate lobe ; Rabbit ; Ontogenesis ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The development of the intermediate lobe of the rabbit pituitary was investigated by light and electron microscopy and by using immunocytological techniques. The first immunoreactive melanotrophic cells were detected at the fetal day 17 in the dorsal zone of Rathke's pouch epithelium facing the neural lobe; this coincided ultrastructurally with the appearance in this area of a few cells exhibiting secretory vesicles and granular condensations in the Golgi saccules. The differentiation of the gland probably required an infundibular inductive effect. Secretory cells increased in number following a dorsoventral gradient during the next fetal and neonatal stages until postnatal day 20, the stage at which the intermediate lobe exhibited its definitive organization. The gland innervation occurred during the first days after birth. The advent of these oxytocin- and neurophysin-immunoreactive fibres coincided with an obvious stimulation of the synthetic activity of the melanotrophic cells. The possible neurotrophic effect of these cells on their innervating system remains to be established.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00208199
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  • 7
    Electronic Resource
    Electronic Resource
    Cell wall formation in zoospores of Allomyces arbuscula (1974)
    Springer
    Archives of microbiology 98 (1974), S. 147-158 
    Details
    ISSN: 1432-072X
    Keywords: Allomyces ; Zoospores ; Cell Wall ; Wall Formation ; Lomasome ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ultrastructural observations on encysting haploid zoospores of Allomyces arbuscula are presented with special reference to cell wall deposition. Multivesicular bodies are observed in the cytoplasm of zoospores 15 min after inoculation, lomasomes after 30 min and fine membrane profiles between the plasmalemma and the cyst wall are observed after 4 h indicating a possible system for secretion of cell wall components.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425277
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  • 8
    Electronic Resource
    Electronic Resource
    Mitochondrial abnormalities of muscle tissue in mice with juvenile visceral steatosis associated with systemic carnitine deficiency (1995)
    Springer
    Virchows Archiv 426 (1995), S. 271-279 
    Details
    ISSN: 1432-2307
    Keywords: JVS mouse ; Systemic carnitine deficiency ; Mitochondrial abnormality ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A mouse with juvenile visceral steatosis (the JVS mouse) has been recognized as a novel animal model for systemic carnitine deficiency. We examined cardiac, skeletal and smooth muscle cells in JVS and control mice by light and electron microscopy. Cardiac and skeletal muscle cells of these mice at 4 weeks of age exhibited a ragged-red appearance after trichrome staining. Electron microscopy, demonstrated increased numbers of mitochondria and lipid droplets in the cells. Compression or distortion of the myofibril bundles, primarily due to the increased number of mitochondria, suggests the possible existence of a functional disturbance of the cardiac and skeletal muscle. In the urinary bladder, only one or two large lipid droplets and slightly increased number of mitochondria were recognized in the perinuclear region of the smooth muscle cells. At 8 weeks of age, the mouse enzyme histochemistry specific for mitochondria, such as cytochrome c oxidase and succinic dehydrogenase, and oil red O staining, confirmed further increases in the number of mitochondria and lipid droplets in the heart. However, the accumulation of these organelles in the skeletal and smooth muscle cells was no greater than that noted in JVS mice at 4 weeks of age. In the cardiac muscle cells, autolysosomes or autophagic vacuoles containing electron-dense membranous, lamellar or whorled structures closely associated with mitochondria and pseudoinclusion bodies in the nucleus were recognized, and bundles of myofibrils were buried under numerous mitochondria, suggesting the existence of disturbed contractile function in the heart of JVS mice. These results indicate that this murine strain associated with systemic carnitine deficiency exhibits a generalized mitochondrial abnormality in the muscle system especially in the heart.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191365
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  • 9
    Electronic Resource
    Electronic Resource
    The hypothalamo-hypophysial system of hypophysectomized rats (1974)
    Springer
    Cell & tissue research 155 (1974), S. 541-554 
    Details
    ISSN: 1432-0878
    Keywords: Hypophysectomized rats ; Reorganized stalk ; Ultrastructure ; Salt load
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The reorganized stalk of hypophysectomized rats, either “intact” or subjected to a 6-day salt load, has been studied by light and electron microscopy. PAF-positive neurosecretory material is seen mainly in the periphery of the reorganized stalk, usually in 2–5 μ nerve swellings of animals operated two months before. Nerve swellings make contact with a dense capillary network. Three types of nerve fibres and their swellings are distinguished according to the size of elementary granules. Peptidergic A1 and A2 types contain granules with a maximum diameter of 170 and 130 nm respectively; monoaminergic B type fibres have granules of 100 nm in diameter. A1 type axonal swellings are the most numerous and can be tentatively divided into four subtypes according to their ultrastructure and relationships with capillaries and pituicytes. Swellings of types b and c, making direct contact with capillaries or pituicyte perikarya, usually contain few granules and numerous synaptic vesicles arranged frequently in clusters in the “active” zones. It is assumed that they are especially active in the release of peptide neurohormones. It is also hypothesized that in the hypophysectomized rats peptide neurohormones are released not only directly into the pericapillary space but also into the intercellular cleft, especially at sites of neuron-pituicyte junctions. Nerve swellings of type a, separated from the pericapillary space by thin pituicyte processes, usually contain numerous neurosecretory granules. It is suggested that vascular “end-feet” of pituicytes may somehow reduce neurohormone release. The depletion of PAF-positive material as well as the decreased granule content even after a salt load of only 1 per cent shows that the reorganized stalk is functional as a neurohaemal organ, but has a diminished hormone reserve. This, together with a primitive cytomyeloangioarchitecture speaks for morphological and functional imperfection of the reorganized stalk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227014
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  • 10
    Electronic Resource
    Electronic Resource
    Characterization of dendritic cells, isolated from normal and stimulated lymph nodes of the rat (1985)
    Springer
    Cell & tissue research 242 (1985), S. 469-474 
    Details
    ISSN: 1432-0878
    Keywords: Lymph node ; Antigen stimulation ; Dendritic cells ; Enzyme- and immunocytochemistry ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Non-lymphoid dendritic cells were isolated from normal and paratyphoid vaccine-stimulated lymph nodes draining the rat skin. They were studied using enzymecytochemical, immunocytochemical and electron-microscopical methods. These cells had an irregular outline and an eccentrically situated nucleus. All showed acid phosphatase activity in a central area and expressed Ia antigen on the plasma membrane. Birbeck granules were exclusively present in dendritic cells isolated from lymph nodes in the induction phase of the immune response. This observation concurs with the presence of Birbeck granules in interdigitating cells in situ during the same period of the immune response. It is concluded that the dendritic cells are the in-vitro equivalents of the non-actively phagocytizing population of interdigitating cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225411
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