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  • 1
    Electronic Resource
    Electronic Resource
    Aggregation of Neurofilaments in NF-L Transfected Neuronal Cells: Regeneration of the Filamentous Network by a Protein Kinase C Inhibitor (1996)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 67 (1996), S. 0 
    Details
    ISSN: 1471-4159
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract: Cytoplasmic inclusion bodies that are accumulations of neurofilaments are the pathological hallmark of many neurodegenerative diseases and have been produced in transgenic mice by overexpression of mouse (NF-L and NF-M; light and medium chains, respectively) and human (NF-M and NF-H; medium and heavy chains, respectively) neurofilament subunits. This report describes a neuronal culture model in which human NF-L was overexpressed to produce cytoplasmic accumulations of neurofilaments within cell bodies concomitant with the collapse of the endogenous neurofilament network. Electron microscopy showed that, within accumulations, neurofilaments retained a filamentous structure. The culture model thus provides a novel system in which the effect on neurofilament accumulations of manipulating protein phosphorylation can be studied. Treatment of cells containing neurofilament accumulations with bisindolylmaleimide, a specific protein kinase C inhibitor, resulted in regeneration of the filamentous network; this effect was not due to a change in the level of transfected NF-L expression. These findings lend support to the suggestion that an impairment in the regulation of protein phosphorylation may lead to the accumulation of neurofilaments seen in neurodegenerative disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1471-4159.1996.67051997.x
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  • 2
    Electronic Resource
    Electronic Resource
    Operating stability of light-emitting polymer diodes based on poly(p-phenylene vinylene) (1997)
    Woodbury, NY : American Institute of Physics (AIP)
    Applied Physics Letters 71 (1997), S. 34-36 
    Details
    ISSN: 1077-3118
    Source: AIP Digital Archive
    Topics: Physics
    Notes: We have developed modified poly(p-phenylene vinylene) (PPV) to improve its performance in organic electroluminescent devices. Encapsulated single-layer devices with this PPV on indium–tin oxide and a calcium cathode have been operated in air for 〉7000 h at 20 °C and 〉1100 h at 80 °C without noticeable degradation. Devices fabricated with this PPV, a conducting polymer layer as anode, and a sputtered low work-function alloy as cathode have efficiencies of over 2 lm/W, brightnesses of 〉600 Cd/m2 at 4 V, and lifetimes of 〉1400 h driven at ∼100 Cd/m2 initial brightness. © 1997 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.119483
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  • 3
    Electronic Resource
    Electronic Resource
    Delayed paraplegia following spinal anaesthesia (1996)
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 51 (1996), S. 0 
    Details
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report an extremely rare complication of regional anaesthesia, a spinal subdural haematoma, which resulted in permanent neurological damage occurring 8 days after dural puncture at T12–L1. Although spinal subdural haematoma following spinal anaesthesia and lumbar puncture has been described before, this is the first report of this complication occurring after dural puncture using a 25G atraumatic pencil point (Whitacre) needle. Contributory factors might have been the perioperative intermittent low dose aspirin therapy and the fact that spinal anaesthesia was performed at the T12-L1 level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2044.1996.tb13644.x
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  • 4
    Electronic Resource
    Electronic Resource
    Hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a Pseudomonas species (1997)
    Springer
    Archives of microbiology 166 (1997), S. 421-424 
    Details
    ISSN: 1432-072X
    Keywords: Key words Nitrification ; Hydroxylamine-cytochrome c oxidoreductase ; Ammonia monooxygenase ; Denitrification ; Pseudomonas sp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera Pseudomonas, Moraxella, Arthrobacter and Aeromonas. A hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the Pseudomonas and Aeromonas spp. and in total soluble fractions of the Arthrobacter sp. A monomeric 19-kDa non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the Pseudomonas species and shown to be similar to hydroxylamine-cytochrome c oxidoreductase of Paracoccus denitrificans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01682991
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  • 5
    Electronic Resource
    Electronic Resource
    Binding properties of SH3 peptide ligands identified from phage-displayed random peptide libraries (1996)
    Springer
    Molecular diversity 2 (1996), S. 5-12 
    Details
    ISSN: 1573-501X
    Keywords: SH3 domains ; Combinatorial peptide libraries ; cDNA expression libraries
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Combinatorial libraries have yielded high-affinity ligands for SH3 domains of a number of different proteins. We have shown that synthetic peptides containing these SH3 ligand sequences serve as specific probes of SH3 domains. Direct binding of the N-terminal biotinylated peptide ligands was conveniently detected in ELISA, filter-blotting, and dot-blotting experiments with the use of streptavidin-conjugated enzymes. In some cases, detection of peptide-SH3 interactions required that the biotinylated peptides first were preconjugated with streptavidin to form a multivalent complex. Interestingly, these nominally tetravalent SH3 peptide ligands cross-react to varying degrees with different SH3 domains. We have used such complexes to screen λcDNA expression libraries and have isolated clones that encode both known and novel SH3-domain-containing proteins. Based on the success of this methodology, we propose a general strategy by which ligands of a modular domain-containing protein can be isolated from random peptide libraries and used to screen cDNA expression libraries systematically for novel modular domain-containing proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718694
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  • 6
    Electronic Resource
    Electronic Resource
    Liquid phase behaviour in nonstoichiometric calcium-doped lanthanum chromites (1996)
    Springer
    Journal of materials science 31 (1996), S. 157-163 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The sinterability of (La,Ca)CrO3 is analysed by comparing the liquid phase behaviour in A-site excess and A-site deficient compositions (referring to the ABO3 formula). The analysis shows that a series of A-site excess and deficient compositions form distinct liquid phases belonging to the CaO-Cr2O3 phase system. Although both series experience grain growth and densification due to the presence of a liquid phase, the A-site excess compositions exhibit greater shrinkage and sinter to closed porosity, whereas the A-site deficient compositions remain porous. During the final stages of sintering, surplus liquid from the A-site excess material exudes to the free-surface forming a layer of uniform thickness. By comparison, the liquid in the A-site deficient composition segregates to the free-surface forming islands and leaving porous regions in the bulk matrix. It is concluded that the different liquid phase compositions in the A-site excess and A-site deficient (La,Ca)CrO3 have dissimilar wetting characteristics during the later stages of sintering. The A-site deficient liquid forms a premature solid phase, which accounts for the difference in sintering behaviour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00355140
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  • 7
    Electronic Resource
    Electronic Resource
    Structure and phase transformation of lanthanum chromate (1996)
    Springer
    Journal of materials science 31 (1996), S. 551-557 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Lanthanum chromate (LaCrO4) was synthesized as a low temperature ( 〈 700 °C) intermediate to perovskite-type, lanthanum chromite (LaCrO3). The lattice parameters, atom positions and bond lengths determined from X-ray powder diffraction show that LaCrO4 forms a monazite-type crystal structure. Lanthanum chromate forms a solid solution with Ca, having a solubility between 10 and 20 at%. Thermal analysis shows that at ambient oxygen pressure, LaCrO4 transforms to LaCrO3 at a temperature near 700 °C. It also indicates that (La,Ca)CrO4 initially transforms to LaCrO3 and CaCrO4 with the subsequent formation of a (La,Ca)CrO3 solid solution. Evidence of Ca segregation to the surface of (La,Ca) CrO3 particles is given by Auger electron spectroscopy and scanning electron microscopy.[/p]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01139176
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  • 8
    Electronic Resource
    Electronic Resource
    Immunolabeled microtubules and microfilaments are visible in multiple cell layers of rye root tip sections (1996)
    Springer
    Protoplasma 191 (1996), S. 215-219 
    Details
    ISSN: 1615-6102
    Keywords: Laser scanning confocal microscopy ; Multiple cell layers ; Plant microtubules ; Plant microfilaments ; Roots ; Tissue clearing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A protocol was developed to observe plant microtubules and actin microfilaments in large tissue samples without physical sectioning. Rye (Secale cereale L. cv. Rymin) root tip pieces from two-day-old seedlings were fixed and processed for immunolabeling. Incubation times of 24–48 h were required to insure adequate penetration of fixatives, antibodies, and washing buffers. Clearing of the tissue with methyl salicylate reduced background auto-fluorescence that would otherwise interfere with the resolution of cytoskeletal structures. Microtubules or microfilaments in 5–7 cell layers were visualized using the optical-sectioning capability of laser scanning confocal microscopy (LSCM) and projected as three-dimensional images. The three-dimensional character of the cytoskeletal elements is retained when viewing stained cells of intact tissue. The net-like character of a microfilament array radiating out from a single point into the cytoplasm is maintained when the cells are stained in intact root tip pieces and imaging is accomplished in situ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01281819
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  • 9
    Electronic Resource
    Electronic Resource
    ELASTIC ANALYSIS OF BURIED STRUCTURES SUBJECT TO THREE-DIMENSIONAL SURFACE LOADING (1996)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 20 (1996), S. 331-349 
    Details
    ISSN: 0363-9061
    Keywords: elasticity ; buried pipes ; surface heading ; soil-structure interaction ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A method is presented which may be used to compute the displacements, strains and moments (both in-plane and transverse) in buried structures such as pipelines and culverts subjected to longitudinal bending. This type of bending can occur if a surface loading such as a vehicular loading or an embankment loading is applied to the soil above the pipe or culvert.Fourier transforms are used to reduce the three-dimensional problem to one involving only two spatial directions, thereby reducing the data preparation and computation time. Conventional finite element analysis is used to approximate the field quantities in the transformed two-dimensional plane. Two Fourier integral element types have been developed which have many applications in geotechnical engineering.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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