ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Ion-pair chromatography
;
Peak compression
;
Remoxipride metabolite
;
Conjugate
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary FLA 908 was identified as a metabolite of remoxipride in human urine after enzymatic hydrolysis. The identity was proven by comparison of its retention time in LC and its UV and mass spectra to authentic FLA 908. The concentration of FLA 908 in human urine was determined using chromatographic conditions where a peak compression effect was obtained. This effect, giving an extremely narrow peak for FLA 908, made it possible to determine low concentrations of the compound in enzymatically hydrolyzed urine. The limit of quantitation was improved more than by a factor of 5 compared to conventional chromatography and the precision was good with a coefficient of variation of 〈5%. Less than 1% (0.44–0.91%) of the administered remoxipride dose was found to be excreted as conjugated FLA 908 while only trace amounts (≤0.01%) of nonconjugated FLA 908 were seen.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02268775
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