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  • 1
    Electronic Resource
    Electronic Resource
    Incompatibility and resistance to woolly apple aphid in apple (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The study investigated the reported linkage of the locus for resistance to woolly apple aphid with the locus for incompatibility. Apple seedlings from the cross ‘Northern Spy’(heterozygous for resistance) בTotem’(susceptible) were scored for resistance, and for incompatibility genotype, by analysis of stylar ribonucleases, and for Got-1, the isoenzyme marker for incompatibility. Cosegregation analysis provided no evidence that the loci for resistance and incompatibility are linked. Two rootstock cultivars,‘M9’and ‘Merton 789′, which in early work had been reported to give poor set in crosses with ‘Northern Spy’, were found to have the same incompatibility genotype as ‘Northern Spy’, namely S1S3.‘M4’and ‘Irish Peach’, two other cultivars that had given poor set when crossed on to ‘Northern Spy’, appeared to be homozygous at the incompatibility locus and to have the genotypes S3S3 and S1S1, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00442.x
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  • 2
    Electronic Resource
    Electronic Resource
    Human T-Cell Epitopes on the Mycobacterium tuberculosis Secreted Protein MPT64 (1996)
    Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 43 (1996), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Mycobacterium tuberculosis secretes a number of proteins into the extracellular envirornment, some of which are restricted to the M. tuberculosis complex. These proteins are targets for T- and B-cell immune responses in tuberculosis (TB) patients and their contacts. The authors have mapped the immunogenic regions of the MPT64 protein of M. tuberculosis using peripheral blood mononuclear cells (PBMC) from TB patients and a set of overlapping peptides encompassing the complete sequence of the protein. T-cell epitopes which induced proliferation or interferon-gamma (IFN-γ) release were distributed over the full length of the protein. A C-terminal region of the protein, however, contains sequences recognized in the context of multiple HLA-DR phenotypes by more than 80% of the subjects tested. The nature of the T-cell response was further investigated by generating MPT64-specific T-cell lines. These lines also identified the T-cell epitopes in the C-terminal region of the protein. On stimulation with antigen the lines secreted IFN-γ, but not interleukin 4 (IL-4). A minority of TB patients (6/32) mounted an antibody response to MPT64. Sera from half (3/6) of these identified two linear antibody binding sites. These results confirm the significance of this protein in the immune response to tuberculosis infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3083.1996.d01-260.x
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  • 3
    Electronic Resource
    Electronic Resource
    Wide spectrum screening keratin as a marker of metaplastic spindle cell carcinoma of the breast: an immunohistochemical study of 24 patients (2002)
    Oxford UK : Blackwell Science Ltd
    Histopathology 40 (2002), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Wide spectrum screening keratin as a marker of metaplastic spindle cell carcinoma of the breast: an immunohistochemical study of 24 patients Aims: Metaplastic spindle cell carcinomas may be difficult to distinguish histologically from other spindle cell lesions in the breast. Variable staining with cytokeratin immunomarkers has been reported for metaplastic carcinomas. We evaluated the diagnostic utility of anti-cytokeratin polyclonal antibody, wide spectrum screening keratin, to assess spindle cell breast lesions. Methods and results: Twenty-four patients with spindle cell breast carcinoma and 31 patients with benign or malignant spindle cell tumours were studied using a panel of antibodies directed against multiple cytokeratins (AE1/AE3, CAM5.2, wide spectrum screening keratin), epithelial membrane antigen (EMA), and vimentin. Sites of origin for the 31 controls included breast, bone, and soft tissue. All but one (95.8%) metaplastic carcinomas stained positively with wide spectrum screening keratin. Only rare or focal immunoreactivity was observed with AE1/AE3 in four cases; however, sensitivity of AE1/AE3 was improved in 13 cases using steam EDTA as an antigen retrieval technique. Three cases were immunoreactive with CAM5.2 and eight cases were immunoreactive with EMA. All control cases lacked immunoreactivity with the cytokeratin panel and EMA. The spindle cells in the metaplastic breast tumours (88%) and in the controls (97%) stained with vimentin. Conclusions: Wide spectrum screening keratin may be the most useful and convenient antibody in differentiating metaplastic spindle cell carcinoma from other spindle cell lesions in the breast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2559.2002.01417.x
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  • 4
    Electronic Resource
    Electronic Resource
    Cavitation in superfluid helium-4 at low temperature (1998)
    Springer
    The European physical journal 2 (1998), S. 381-391 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 67.20.+k Quantum effects on the structure and dynamics of nongenerate fluids (e.g., normal phase liquid 4He) - 47.55.Bx Cavitation - 64.60.Qb Nucleation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: We have studied the nucleation of bubbles in pure superfluid helium-4 at temperatures down to 65 mK. We have found that the nucleation is a stochastic process, and that at temperatures below 600 mK the nucleation rate is independent of temperature. These results are consistent with the assumption that the nucleation takes place via quantum tunneling.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100510050261
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  • 5
    Electronic Resource
    Electronic Resource
    Shot-noise statistics in diffusive conductors (2002)
    Springer
    The European physical journal 27 (2002), S. 393-398 
    Details
    ISSN: 1434-6036
    Keywords: PACS. 72.70.+m Noise processes and phenomena – 73Electronic structure and electrical properties of surfaces, interfaces, thin films, and low-dimensional structures – 71.10.Fd Lattice fermion models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract: We study the full probability distribution of the charge transmitted through a mesoscopic diffusive conductor during a measurement time Δ. We have considered a semi-classical model, with an exclusion principle in a discretized single-particle phase-space. In the large Δ limit, numerical simulations show a universal probability distribution which agrees very well with the quantum mechanical prediction of Lee et al. [Phys. Rev. B 51, 4079 (1995)] for the charge counting statistics. Special attention is given to its third cumulant, including an analysis of finite size effects and of some experimental constraints for its accurate measurement.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1140/epjb/e2002-00170-5
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  • 6
    Electronic Resource
    Electronic Resource
    Crowned dens syndrome: a manifestation of hydroxy-apatite rheumatism (1995)
    Springer
    Acta neurochirurgica 135 (1995), S. 126-130 
    Details
    ISSN: 0942-0940
    Keywords: Crowned odontoid ; hydroxy-apatite rheumatism ; occipitocervical region ; odontoid process
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Crowned dens syndrome is defined as an association of acute cervical pain and calcifications in the peri-odontoid space. The authors report one case of this rare localization of hydroxy-apatite rheumatism and review 12 similar cases in the literature. This disease affects only adult females. Patients present with inflammatory signs, can be treated with non-steroid anti-inflammatory drugs and recover without sequela. Calcium pyrophosphate dihydrate deposition can also lead to this syndrome. Other perioodontoid calcifications and ossifications, usually asymptomatic, appear only as a radiologically crowned dens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02187755
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  • 7
    Electronic Resource
    Electronic Resource
    Cauda equina paraganglioma with subsequent intracranial and intraspinal metastases (1996)
    Springer
    Acta neurochirurgica 138 (1996), S. 475-479 
    Details
    ISSN: 0942-0940
    Keywords: Cauda equina ; cerebrospinal fluid metastases ; paraganglioma ; spinal tumour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of cauda equina paraganglioma is described; subsequent intracranial and intraspinal metastases occurred after partial resection and adjunctive radiotherapy. Cerebrospinal fluid dissemination is a rare complication of spinal paragangliomas. Factors predictive of this unusual biological behaviour are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01420312
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  • 8
    Electronic Resource
    Electronic Resource
    Giant central neurocytoma with tetraventricular and extra-axial extension (1995)
    Springer
    Acta neurochirurgica 133 (1995), S. 95-100 
    Details
    ISSN: 0942-0940
    Keywords: Central neurocytoma ; intraventricular tumour ; neuronal tumour ; synaptophysin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The central neurocytoma is a recently recognized benign intraventricular tumour of young adults. The authors report a unique case of a panventricular neurocytoma with extension to the interpeduncular and prepontique cisterns which developed in a 35-year-old woman with a 7-year history of headaches and amenorrhea. They review the different pathological and topographical patterns of previously published neurocytomas and discuss the histogenesis of this rare tumour.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404957
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  • 9
    Electronic Resource
    Electronic Resource
    RFLP and RAPD markers linked to the rosy leaf curling aphid resistance gene (Sd1) in apple (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 528-533 
    Details
    ISSN: 1432-2242
    Keywords: Key words Apple ; Dysaphis devecta ; Insect resistance ; Marker-assisted selection ; Molecular markers ; Plant breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Sd 1 is a dominant gene for resistance to biotypes 1 and 2 of the rosy leaf curling aphid, Dysaphis devecta Wlk., which can cause economic damage to apple trees. This report describes the identification of three RFLP and four RAPD markers linked to Sd 1 in a cross between the D. devecta susceptible variety ‘Prima’ (sd 1 sd 1) and the resistant variety ‘Fiesta’ (Sd 1 sd 1). Potted trees were artificially infested in the glasshouse, and the ratio of resistant:susceptible plants supported the hypothesis that the resistance was under the control of a single dominant gene. The position of the gene was mapped to a single locus on a ‘Fiesta’ chromosome, within 2 cM of three tightly linked RFLP markers (MC064a, 2B12a and MC029b); the four RAPD markers were located further away (between 13 and 46 cM). This is the first report of molecular markers for an aphid resistance gene in tree fruit crops. The potential application of these markers in a marker-assisted resistance breeding programme is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050447
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  • 10
    Electronic Resource
    Electronic Resource
    Multiple field and glasshouse assessments increase the reliability of linkage mapping of the Vf source of scab resistance in apple (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 699-708 
    Details
    ISSN: 1432-2242
    Keywords: Key words Malus ; Apple ; Venturia ; Scab resistance ; Linkage mapping ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Apple scab, caused by the fungus Venturia inaequalis (Cke.) Wint., is an important disease in commercial apple production. A mapping population of 155 individuals, derived from a cross between the apple varieties ‘Prima’ (resistant)בFiesta’ (susceptible), was scored for response to the disease in replicated field and glasshouse trials throughout Europe. Twenty data sets were selected and cluster analysis was used to form a consensus score for the population fitting a 1 : 1 segregation ratio of resistance:susceptibility. The progeny were scored with molecular markers. A detailed map covering 54 cM of the ‘Prima’ linkage group containing the Vf gene for scab resistance was constructed using 24 molecular markers linked to the resistance gene. One isoenzyme marker (Pgm-1), six RFLP markers and 17 RAPD markers formed a linkage group with the consensus measure of resistance to scab. Four marker bridges were established with the corresponding ‘Fiesta’ linkage group with additional markers (one isozyme, one RFLP, three RAPD and one AFLP). A low chi-square value indicated a good fit of the marker ordering, which was in close agreement with previously reported linkage positions for some of the markers and Vf. Differences were observed in the ability of different scoring methods to resolve susceptible and resistant classes. The results obtained for the consensus classification of resistance to scab for the population may suggest the presence of virulent inocula at some sites, which could overcome the Vf gene for resistance. The consequences of relying on individual scoring occasions for studying Vf scab resistance are discussed in the context of linkage analysis, conventional breeding selection, and marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050791
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