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  • 1
    Electronic Resource
    Electronic Resource
    Effect of leaf surface wetness and wettability on photosynthesis in bean and pea (2004)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 27 (2004), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Leaf surface wetness that occurs frequently in natural environments has a significant impact on leaf photosynthesis. However, the physiological mechanisms for the photosynthetic responses to wetness are not well understood. The responses of leaf CO2 assimilation rate (A) to 72 h of artificial mist of a wettable (bean; Phaseolus vulgaris) and a non-wettable species (pea; Pisum sativum) were compared. Stomatal and non-stomatal limitations to A were investigated. A 28% inhibition of A was observed in the bean leaves as a result of a 16% decrease in stomatal conductance and a 55% reduction in the amount of Rubisco. The decrease of Rubisco was mainly due to its partial degradation. In contrast to the bean leaves, a 22% stimulation of A was obtained in the 72 h mist-treated pea leaves. Mist treatment increased stomatal conductance by 12.5% and had no effect on the amount of Rubisco. These results indicated that a positive photosynthetic response to wetness occurred only in non-wettable species and is due to the change in stomatal regulation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2004.01154.x
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  • 2
    Electronic Resource
    Electronic Resource
    Rabeprazole, amoxycillin and low- or high-dose clarithromycin for cure of Helicobacter pylori infection (2000)
    Oxford UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 14 (2000), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Rabeprazole sodium is a proton pump inhibitor.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To evaluate the efficacy and safety of 1-week triple therapy with rabeprazole, amoxycillin and clarithromycin for the eradication of Helicobacter pylori.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:A total of 100 subjects with H. pylori were randomly divided into two groups of 1-week triple therapy with rabeprazole 10 mg b.d., amoxycillin 750 mg b.d. and either clarithromycin 200 mg b.d. (RAC400, n=50) or clarithromycin 400 mg b.d. (RAC800, n=50). Endoscopic examination with four biopsies (two specimens from the antrum and two from the gastric body) was performed. The status of H. pylori infection was determined using culture and histology (Giemsa stain) of the biopsy specimens. Sensitivity to clarithromycin was determined using the E-test: MIC 〉 8 g/mL was considered to be resistant, whereas MIC 〈 2 g/mL was considered to be sensitive. Cure was defined as no evidence of H. pylori infection 1 month after completion of treatment.〈section xml:id="abs1-4"〉〈title type="main"〉Results:There were no significant differences in the clinical characteristics of the two groups. Eradication rates (intention-to-treat and per protocol, respectively) were: RAC400: 86% (95% CI: 76–95%) and 89% (95% CI: 80–97%); RAC800: 94% (95% CI: 87–100%) and 97% (95% CI: 94–100%). There was no significant difference between the eradication rates of either regimen. Three subjects with failed eradication in the RAC400 group were all infected with a clarithromycin-resistant strain before beginning the therapy. Haemorrhagic colitis was the only severe adverse event, which was observed in one patient in the RAC800 group.〈section xml:id="abs1-5"〉〈title type="main"〉Conclusion:One-week triple therapy with rabeprazole, amoxycillin and low-dose clarithromycin is effective for the eradication of H. pylori infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2000.00810.x
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  • 3
    Electronic Resource
    Electronic Resource
    A novel A97P amino acid substitution in α-galactosidase A leads to a classical Fabry disease with cardiac manifestations (2002)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 147 (2002), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Fabry disease results from a genetic deficiency of α-galactosidase A (GLA) activity. Phenotype–genotype correlations in this condition have not as yet been fully elucidated. Objectives To report a case of a male patient with classical Fabry disease and his mother, a heterozygous female with Fabry disease, showing cardiac involvement, and to identify the underlying GLA gene mutation in this particular phenotype. Patients/methods Genomic DNA was extracted from the patient, his mother and the unaffected family members. Biopsy specimens of skin, heart and kidney were examined using light and electron microscopy. The mutation was identified by polymerase chain reaction and direct sequencing and was confirmed by restriction enzyme fragment length polymorphism. Results The G→C transversion was identified in codon 97 of the GLA gene and resulted in an A97P amino acid substitution that was a novel pathogenic GLA gene mutation. The male patient who had classical Fabry disease was hemizygous and his mother was heterozygous for this mutation. Conclusions These results indicate that the A97P amino acid substitution in GLA might tend to induce classical Fabry disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2002.04902.x
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  • 4
    Electronic Resource
    Electronic Resource
    Fractionation of Two Metabolically Distinct Classes of Ribonucleic Acids in Animal Cells and its Bearings on Cancer (1960)
    [s.l.] : Nature Publishing Group
    Nature 186 (1960), S. 215-217 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] RECENTLY we have shown1 that the treatment of suspensions or homogenates of normal and malignant animal cells with aqueous phenol may lead to a fractionation of their ribonucleic acids into two components differing markedly in the nucleotide composition and the rate of uptake of phosphorus-32. The ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/186215a0
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  • 5
    Electronic Resource
    Electronic Resource
    Elektrische Untersuchungen am Nervus ampullaris lateralis bei Kaninchen (1960)
    Springer
    European archives of oto-rhino-laryngology and head & neck 177 (1960), S. 31-38 
    Details
    ISSN: 1434-4726
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Autoren legten N. amp. lat. des Kaninchens frei, führten dort die Mikro-elektrode ein und registrierten die Potentialänderung nach der calorischen Reizng des Labyrinthes. Abgesehen von der bereits bekannten Zu- und Abnahme der Grundaktivität konnten die negativen Potentialänderungen registrieert werden. Im Photogramm wurde die Elektronystagmographie mit der Potentialänderung gleichzeitig registriert, und in jedem Falle, wenn das Potential auftrat, wurde Nystagmus beobachtet. Die Genese dieser Potentialänderungen konnte noch nicht festgestellt werden, aber es ist anzunehmen, daß sich die Potentiale als eien Erscheinung im peripheren Labyrinth finden.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02105821
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  • 6
    Electronic Resource
    Electronic Resource
    Molecular analysis of the human orosomucoid gene ORM1*Q0köln responsible for incompatibility in a German paternity case (2000)
    Springer
    International journal of legal medicine 114 (2000), S. 114-117 
    Details
    ISSN: 1437-1596
    Keywords: Key words Isolated exclusion ; Null allele ; Orosomucoid gene ; Paternity case ; Reverse ; homozygosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract In a German paternity test, an alleged father was excluded only by reverse homozygosity of ORM1 phenotypes (mother ORM1 S, child ORM1 S and alleged father ORM1 F1) out of the 28 classical and DNA markers investigated. Without the ORM1 system the biostatistical probability of paternity was calculated to exceed 99.9999%. The intensity of the immunoprinted bands of the ORM1 protein for the child and alleged father after isoelectric focusing appeared to be reduced to about half. To identify a possible null allele, gene-specific amplification followed by single-strand conformation polymorphism and sequencing analyses were carried out. Deletion of one of the two copies of a 4 bp direct repeat sequence (GTCT) in exon 4 of the consensus sequence of ORM1*F1 was observed in the child and alleged father. Thus, the sharing of a rare mutant gene, ORM1*Q0köln, increased the probability of paternity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004149900118
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  • 7
    Electronic Resource
    Electronic Resource
    Proinsulin C peptide obviates sympathetically mediated suppression of splenic lymphocyte activity in rats (2000)
    Springer
    Diabetologia 43 (2000), S. 1512-1517 
    Details
    ISSN: 1432-0428
    Keywords: Keywords Atropine ; C peptide ; CRF ; footshock ; parasympathetic nerve ; sympathetic nerve.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Aims/hypothesis. To investigate whether proinsulin C peptide influences sympathetic nerve activity directly or indirectly through parasympathetic nerve activity. Methods. The proliferative response of splenic lymphocytes to Concanavalin A (ConA response) which is known to be suppressed by subjection of rats to footshock or intracerebroventricular injection of corticotropin-releasing factor through sympathetic nerve activation was measured. Effect of C peptide alone or before subjection to footshock or injection of corticotropin-releasing factor was examined. Results. Intraperitoneal injection of C peptide was without effect on the basal ConA response, while subjection to footshock or injection of corticotropin-releasing factor lowered it. In contrast, prior injection of C peptide obviated the footshock and corticotropin-releasing factor-induced suppression of the response. When given intracerebroventricularly, C peptide was also effective at much smaller doses. Prior injection of atropine cancelled the C-peptide effects. Conclusion/interpretation. Our results indicate that C peptide counteracts the sympathetic nerve-mediated suppression of splenic lymphocyte proliferation in an atropine-sensitive manner. Thus, C peptide probably activates the parasympathetic nervous system through the afferent mechanism, that in turn antagonizes the sympathetic nerve-mediated suppression of splenic lymphocyte functions. [Diabetologia (2000) 43: 1512–1517]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001250051562
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  • 8
    Electronic Resource
    Electronic Resource
    Survey of Mariner-Like Elements in the Housefly, Musca Domestica (2000)
    Springer
    Genetica 108 (2000), S. 81-86 
    Details
    ISSN: 1573-6857
    Keywords: mariner-like element ; Musca domestica ; transposable element
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The presence of mariner-like elements in four strains of the housefly, Musca domestica, was surveyed by PCR. Using the inverted terminal repeat (ITR) sequences of the Mos 1element as primers, DNAs were successfully amplified from all strains of the housefly. Southern blot analysis indicated that these amplified DNAs were repetitive sequences in the genome of M. domestica. Sequence analyses of cloned PCR products showed that they were 45% identical to the Mos 1element. These fragments appeared to be nonfunctional, because they contained no intact open reading frame (ORF) capable of encoding transposase. We conclude that these DNAs are degraded mariner-like elements (MLEs) in M. domestica. Because these endogenous MLEs in M. domesticado not encode any functional proteins, they probably would not affect the behavior of mariner-based vectors if such were introduced into this species as transformation vectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004076829873
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  • 9
    Electronic Resource
    Electronic Resource
    The rapid identification method of additives in polypropylene by mass spectrometry (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 15 (1971), S. 2065-2072 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: For the rapid identification of additives such as antioxidant, ultraviolet absorber, and slipping agent, polypropylene containing such additives was heated in a tube connected directly to the inlet system of a mass spectrometer. The vapor of the additives evaporating out of the polypropylene was led to the reservoir and then submitted to mass spectrometry to give spectra for identification.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1971.070150901
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  • 10
    Electronic Resource
    Electronic Resource
    The gel permeation chromatography of phenolic compounds (1971)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 15 (1971), S. 2513-2520 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Gel permeation chromatography, using polystyrene gel and tetrahydrofuran as solvent, has been applied to hydroxybenzophenones, esters of salicylic acid, alkylphenols, alkylated methylenediphenols, and phenol-formaldehyde condensation products. The difference between the calculated molecular volume of these phenolic compounds and that obtained by actual determination with GPC has been ascribed to tetrahydrofuran solvation of the phenolic hydroxyl group. Furthermore, it has become clear that THF solvation is affected by the steric hindrance of ortho-substituted phenol and by inactivation of the phenolic hydroxyl group resulting from internal hydrogen bonding.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1971.070151016
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