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  • 1
    Electronic Resource
    Electronic Resource
    Identity of HML-1 Antigen on Intestinal Intraepithelial T Cells and of B-ly7 Antigen on Hairy Cell Leukaemia (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 32 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Immunoprecipitation of radioiodinated hairy cell leukaemia (HCL) cell lysates with monoclonal antibody (MoAb) HML-1. originally reported to recognize intraepithelial T cells, and with MoAb B-ly7, originally reported to react with HCL. led to identical biochemical characteristics. In SDS PAGE under reducing conditions, a major band of 143 kDa. a broad band ranging from 112 to 122 kDa. and two additional faint bands of 175 and 100 kDa could be determined. Deglycosylation of N-linked sugar moieties by treatment of immunoprecipitates with endoglycosidases indicated that the two main protein cores of the antigen are predominantly if not exclusively glycosylated by complex and hybrid types of oligosaccharide chains. Competitive binding inhibition demonstrated that both MoAb are directed against different epitopes. Immunohistochemically. the staining patterns obtained with both MoAb in normal tissues, in T-and B-cell lymphomas, and in HCL were identical except for a single case of HCL which was HML-1−/B-ly-7+, We conclude that MoAb HML-1 and B-1y7 recognize the same antigen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02896.x
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  • 2
    Electronic Resource
    Electronic Resource
    Npm/alk fusion mRNA expression in Hodgkin and Reed–Sternberg cells is rare but does occur: Results from single-cell cDNA analysis (1997)
    Springer
    Annals of oncology 8 (1997), S. 83-87 
    Details
    ISSN: 1569-8041
    Keywords: clonal heterogeneity ; Hodgkin's disease ; NPM/ALK ; single cell PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The translocation t(2;5)(p23;q35) leads to the fusion of thenucleophosmin gene (NPM) on chromosome 5q35 to the recently describedreceptor kinase ALK 2p23. It is characteristic of a subgroup of CD30+large-cell anaplastic non-Hodgkin's lymphoma (ALCL). Since some cases ofHodgkin's disease (HD) and ALCL share common features, a common pathogenesishas been proposed in a report of the expression of NPM/ALK fusion mRNA in11/13 Hodgkin's lymphomas. Patients and methods: We approached this question by micromanipulatoryisolation of single Hodgkin and Reed–Sternberg (H-RS) cells andsubsequent RT-PCR amplification of NPM/ALK fusion cDNA from these singlecells. Results: Specificity of cell selection was shown by the HD-specificpattern of EBV-gene expression in single H-RS cells. In 4 out of 7 cases,NPM/ALK fusion cDNA was detected in the RNA from whole lymph node tissue. In2 out of 9 cases, NPM/ALK fusion sequences were amplified from single H-RScells, albeit in a very low frequency (〈5%). Conclusions: These data indicate that NPM/ALK fusion transcripts donot play an early role in the pathogenesis of HD. Whether the rare expressionof NPM/ALK is the result of clonal heterogeneity or an indication for clonalevolution and progression toward ALCL can only be answered by the repeatedanalysis of indicator cases during the course of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008246719680
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  • 3
    Electronic Resource
    Electronic Resource
    Npm/alk fusion mRNA expression in Hodgkin and Reed–Sternberg cells is rare but does occur: Results from single-cell cDNA analysis (1997)
    Springer
    Annals of oncology 8 (1997), S. 83-87 
    Details
    ISSN: 1569-8041
    Keywords: clonal heterogeneity ; Hodgkin's disease ; NPM/ALK ; single cell PCR
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The translocation t(2;5)(p23;q35) leads to the fusion of the nucleophosmin gene (NPM) on chromosome 5q35 to the recently described receptor kinase ALK 2p23. It is characteristic of a subgroup of CD30+large-cell anaplastic non-Hodgkin's lymphoma (ALCL). Since some cases of Hodgkin's disease (HD) and ALCL share common features, a common pathogenesis has been proposed in a report of the expression of NPM/ALK fusion mRNA in11/13 Hodgkin's lymphomas. Patients and methods: We approached this question by micro manipulatory isolation of single Hodgkin and Reed–Sternberg (H-RS) cells and subsequent RT-PCR amplification of NPM/ALK fusion cDNA from these single cells. Results: Specificity of cell selection was shown by the HD-specific pattern of EBV-gene expression in single H-RS cells. In 4 out of 7 cases, NPM/ALK fusion cDNA was detected in the RNA from whole lymph node tissue. In2 out of 9 cases, NPM/ALK fusion sequences were amplified from single H-RS cells, albeit in a very low frequency (〈5%). Conclusions: These data indicate that NPM/ALK fusion transcripts do not play an early role in the pathogenesis of HD. Whether the rare expression of NPM/ALK is the result of clonal heterogeneity or an indication for clonal evolution and progression toward ALCL can only be answered by the repeated analysis of indicator cases during the course of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008246719680
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  • 4
    Electronic Resource
    Electronic Resource
    Buchbesprechungen (1990)
    Springer
    Naturwissenschaften 77 (1990), S. 597-598 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01133736
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  • 5
    Electronic Resource
    Electronic Resource
    Cathepsin D and E co-expression in sinus histiocytosis with massive lymphadenopathy (Rosai-Dorfman disease) and Langerhans' cell histiocytosis: further evidences of a phenotypic overlap between these histiocytic disorders (1994)
    Springer
    Virchows Archiv 424 (1994), S. 601-606 
    Details
    ISSN: 1432-2307
    Keywords: Cathepsin D ; Cathepsin E ; Rosai-Dorfman disease ; Langerhans' cell histiocytosis ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Nosological classification of sinus histiocytosis with massive lymphadenopathy (SHML; Rosai-Dorfman disease) is difficult, and the normal cellular counterpart of Rosai-Dorfman (RD) cells is uncharacterised. The peculiar S-100+ phenotype of RD cells suggests a relationship with the dendritic cell family. Recent investigations have revealed cathepsin E to be selectively concentrated in antigen-presenting cells, whereas cathepsin D was found to be expressed in cells of macrophage lineage. Cathepsin D and E distribution was investigated by immunohistochemistry in a series of SHML biopsies and in two types of dendritic cell proliferative lesions: dermatopathic lymphadenitis (DL) and Langerhans' cell histiocytosis (LCH). In SHML biopsies, RD cells and monocyte-related elements of the sinuses and pulp coexpressed cathepsin D and E. LCH cells also stained for both these aspartic proteinases. Conversely, in DL cathepsin E and D were localised to separate cells that resembled Langerhans' cells (LC) or macrophages, respectively, in morphology and distribution. Our data outline the peculiar immunophenotype of RD and LCH cells and suggest that caution should be exercised in the identification of their normal cellular counterpart. The common expression of cathepsin D and E and of S-100 protein suggests some phenotypic overlap between SHML and LCH cells, despite their striking morphological divergence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00195773
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  • 6
    Electronic Resource
    Electronic Resource
    In situ expression of β1, β3 and β4 integrin subunits in non-neoplastic endothelium and vascular tumours (1994)
    Springer
    Virchows Archiv 425 (1994), S. 375-384 
    Details
    ISSN: 1432-2307
    Keywords: Endothelium ; Vascular tumours ; Integrins Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Endothelial cells play an important role in adhesive interactions between circulating cells and extracellular matrix proteins. In vitro studies have shown that many of these processes are mediated by a superfamily of αβ heterodimeric transmembrane glycoproteins called integrins. The distribution patterns of β1, β3 and β4 integrin subunits in endothelial cells (EC) in situ were examined immunohistochemically on serial forzen sections of a wide range of non-neoplastic tissues and of vascular tumours, both benign and malignant. Expression of the β1 subunit was a constitutive feature of EC. Among the β1-associated α subunits, α5 and α6 were broadly distributed in EC, irrespective of vessel size and microenvironment. The α3 subunit displayed intermediate levels of expression with a slight preference for small vessel EC. Presence of α1 was confined to EC of capillaries and venules/small veins. Expression of α2 in EC was inconsistent. With rare exceptions, the α4 chain was absent in EC. The β3 and αv subunits were expressed in most EC, though not always concomitantly. In contrast to the β1 chain, however, these integrin subunits were absent in EC of glomerular capillaries and were expressed variably in sinusoidal EC. The β4 chain was evenly present in the great majority of EC, except for those of large vessels. In vascular tumours, the patterns of β1 and α1 to α6 subunit expression generally corresponded to those found in their non-neoplastic counterparts. Expression of β3, αv and β4 chains, however, decreased in neoplasia, especially in angiosarcomas. These data show that EC dispose of broad and at the same time differential repertoires of integrin subunits that presumably reflect vessel-type associated functional differences among these cells. In vascular tumours, the orthologous distribution patterns of β1 and α1 to α6 chains are conserved in most instances while the amounts of β3, αv and β4 subunits expressed in EC tend to decrease in the course of malignant transformation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189575
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  • 7
    Electronic Resource
    Electronic Resource
    Comparative evaluation of integrin α- and β-chain expression in colorectal carcinoma cell lines and in their tumours of origin (1994)
    Springer
    Virchows Archiv 425 (1994), S. 229-236 
    Details
    ISSN: 1432-2307
    Keywords: Colorectal carcinoma ; Integrin receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The integrin family consists of broadly expressed cell surface adhesion receptors, each member of which is composed of a non-covalently linked α/β heterodimer. Integrin receptors are involved in the interaction with matrix proteins and may contribute to invasion and metastasis of carcinomas. To examine the biological role integrins play in colorectal carcinoma we compared the expression of integrin α- and β-subunits in situ and in vitro. Eight newly established cell lines derived from immunohistochemically characterized colorectal carcinomas together with two sublines obtained after nude mouse passage and the commonly used colon carcinoma lines HT-29, SW480, SW620, and COLO 205 were investigated by immunocytochemistry and flow cytometry. The carcinomas in situ expressed α1-, α2-, α3-, α6-, αv-and β1-subunits in variable amounts while being devoid of α4, α5 and β3. The individual integrin profile of the tumour in tissue was essentially maintained in vitro. However, a neo-expression of the α5 chain was found, together with an induction or increase in α1, α2, α3, αv and β1 levels. No decrease in integrin subunit expression was observed. Standard-serum and serum-free medium revealed no striking differences in α- and β-chain expression in the cell lines HT-29 and COLO 205. In serum-free medium, SW480 showed a slight increase of α1 and α5 and a decrease of α3 and αv while SW620 expressed more α1. We conclude that the great variability of adhesion receptor expression of the integrin family in colorectal carcinomas in situ is essentially maintained in vitro, although culture conditions which are only marginally influenced by serum factors unpredictably lead to some increase in expression or even induction of several integrin subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196144
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  • 8
    Electronic Resource
    Electronic Resource
    Expression of CD44 splice variants in human skin and epidermal tumours (1996)
    Springer
    Virchows Archiv 428 (1996), S. 141-149 
    Details
    ISSN: 1432-2307
    Keywords: CD44 variant isoforms ; Skin ; Basal cell carcinoma ; Spindle cell carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Splice variants of the adhesion molecule CD44 (CD44v) are important in the lymphatic spread of rat carcinoma cells. In several human tumours expression of CD44v correlates with tumour progression. However, little is known about the physiological functions of distinct variant exons. Here we report on the immunohistological evaluation of CD44 expression in normal human skin and epidermal tumours which do not metastasise, or do so vary rarely. Frozen tissues were stained with a panel of monoclonal antibodies, recognizing epitopes of the CD44 standard isoform, as well as of variant exons v5, v6, v7, v7–v8 and v10. Stratum basale and spinosum as well as the root shaft of hairs reacted strongly with the whole panel of anti-CD44 antibodies. Stratum corneum, acinar cells of sebaceous and eccrine sweat glands stained with anti-CD44v5, anti-CD44v6 and anti-CD44v7, but not with anti-CD44v10, the latter recognizing the “epithelial isoform” (CD44v8–v10) of CD44. Ductal cells of glands and apocrine glands did not express CD44v. Compared with its expression in normal human skin, CD44v expression was reduced in basal cell carcinoma and squamous cell carcinoma of the skin. This was particularly true of CD44v10. The expression of CD44v in normal skin and dermal appendages indicates that not all combinations of variant exons are involved in tumour progression. Since the epithelial isoform is particularly downregulated in basal cell carcinoma and squamous cell carcinoma of the skin, it is unlikely that exons v8–v10 play a role in tumour progression. Rather, they may be of functional importance in maintenance of the epidermal structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200656
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  • 9
    Electronic Resource
    Electronic Resource
    Immunohistochemical reclassification of anaplastic carcinoma reveals small and giant cell lymphoma (1990)
    Springer
    World journal of surgery 14 (1990), S. 291-294 
    Details
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Trente et un cas de tumeurs malignes de la thyroïde, classifiés à l'origine comme des carcinomes anaplasiques, ont été réexaminés en immunohistochimie pour la présence d'anticorps IgM, IgG, IgA, de cytocératine, calcitonine, lysocyme, et alpha1-antitrypsine en utilisant la méthode péroxydase/antipéroxydase. Les résultats de cette reclassification ont été comparés avec des données cliniques: symptômes cliniques, modalités thérapeutiques, et évolution clinique. On a employé la radiothérapie postopératoire dans 80% des cas et la chimiothérapie dans aucun cas. Sept des 31 tumeurs étaient positives pour les anticorps IgM (n=4), IgG (n=2), et IgA (n=1). Dans tous ces cas, les marqueurs épithéliaux étaient négatifs. De cette manière, on a pu reclasser comme lymphomes malins primitifs non seulement 3 tumeurs à petites cellules mais aussi 4 tumeurs avec des zones de cellules géantes.
    Abstract: Resumen Treinta y un casos de neoplasias malignas de la glándula tiroides originalmente clasificadas como carcinoma anaplásico fueron reexaminados por inmunohistoquímica utilizando métodos de peroxidosa:antiperoxidasa (PAP) para IgM, IgG, IgA, citoceratina, calcitonina, lisocina, y alfa-1-antitripsina. Los resultados de la reclasificación fueron comparados con la información clínica, incluyendo sintomatología, modalidades de tratamiento, y evolución final. Se realizó radioterapia postoperatoria en más de 80% de los casos, quimioterapia en ninguno. Siete de 31 tumores exhibieron tinción positiva para anticuerpos IgM (n=4), IgG (n=2), e IgA (n=1). Todos estos casos fueron negativos para marcadores epiteliales. Sorprendentemente, no sólo la totalidad de los tumores de células pequeñas (n=3) sino también 4 tejidos con áreas predominantes de células gigantes, estuvieron en el grupo de aquellos reclasificados como linfoma maligno.
    Notes: Abstract Thirty-one cases of thyroid malignancies which were originally classified as anaplastic carcinoma were reexamined immunohistochemically using PAP methods (peroxidase:antiperoxidase) for IgM, IgG, IgA, cytoceratin, calcitonin, lysozyme and alpha-1-antitrypsin. The reclassification results were compared with patient data such as clinical symptoms, treatment modalities, and clinical outcome. Postoperative radiotherapy was carried out in more than 80% of cases, chemotherapy in none. Seven of 31 tumors showed a positive staining for IgM (n=4), IgG (n=2), and IgA (n=1) antibodies. All of these cases were negative for epithelial markers. Surprisingly, not only all small cell tumors (n=3) but also 4 tissues with predominantly giant cell areas were among those reclassified as primary malignant lymphoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01658506
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  • 10
    Electronic Resource
    Electronic Resource
    PMA-activation of peripheral blood and tonsillar B lymphocytes induces large adhesive cells reminiscent of large extrafollicular (monocytoid) B cells (1994)
    Springer
    Virchows Archiv 424 (1994), S. 195-204 
    Details
    ISSN: 1432-2307
    Keywords: Extrafollicular B lymphocytes ; Monocytoid B cell ; Phorbol 12-myristate 13-acetate ; CD11c ; CD62L (L-selectin)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Extrafollicular (EF) B lymphocytes differ in size and morphology depending on the lymphatic organ involved and the kind of inflammatory reaction. On reevaluating EF B cells in various sites and conditions we discriminated three forms: a small (lymphoid) and intermediate (centrocytoid), and a large (monocytoid) variant. Immunohistochemically, these variants could be discriminated by their differential expression of adhesion molecules CD62L (L-selectin) and CD11c: small EF B cells were strongly L-selectin+ and CD11c−; intermediate cells were moderately CD62L+ and CD11c−; large cells were faintly CD62L+ or − but expressed CD11c. In 72 h cultures of normal peripheral and tonsillar B cells, cross-linking surface immunoglobulin in the presence of interleukin-2 or interleukin-4 led to formation of clusters in vitro together with an increase in cell size and a slight up-regulation of CD11c, as determined by flow cytometry. Stimulation with phorbol 12-myristate 13-acetate (PMA), however, gave rise to large, plastic adherent cells which also showed strong homotypic adhesion, expressed CD62L at minimal levels and CD11c at comparably highest levels and altogether mimicked the large cell variant of EF B cells. We conclude that EF B cells are subjected to cytokine-induced metamorphosis and that differences in cell size and morphology reflect their state of activation and activation-associated adhesion properties. Our data suggest that EF B cells in all anatomical sites are functionally closely related cells which — possibly mediated by CD11c/CD18 — may become sessile and proliferate locally once activated by appropriate signals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00193500
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