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  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructural Evidence for Transport of Protein across the Epithelium of the Rat Caput Epididymidis (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 513 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb25099.x
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  • 2
    Electronic Resource
    Electronic Resource
    Fraction of H-bonded segments of N-ethylpyrrolidone, oligomeric and polymeric vinylpyrrolidone adsorbed from CHCl3 on silica measured by IR spectrometry (1985)
    Springer
    Colloid & polymer science 263 (1985), S. 372-380 
    Details
    ISSN: 1435-1536
    Keywords: Polymer adsorption ; IR spectrometry ; fraction of adhered segments ; silica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The adsorption of N-ethylpyrrolidone (NEP), oligomeric and polymeric vinylpyrrolidone (OVP, PVP) on silica (Aerosil 200) from CHCl3 solution is investigated by IR spectrometry. The influence of the annealing temperature of the silica on the adsorbed amount and on the fraction of adhered segments was studied. The dependences of the amount adsorbed on solution concentration result for NEP in a Langmuir isotherm for OVP and PVP in high affinity isotherms. The fraction of adhered H-bridged carbonyl groups determined by compensation procedures, the fraction of surface SiOH groups occupied and the multiple interaction quotientQ show different dependences on the amount adsorbed for the measured adsorptives, indicating different interactions of the monomeric segments with the surface groups. This behaviour is explained by comparing the amount adsorbed in saturation with monolayer capacities, surface concentration of SiOH groups and frequency shifts. A splitting of the band of the bound carbonyl groups in a double peak was observed with OVP and PVP, referring to an additional interaction of polymer segments neighboured to specifically bound segments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01410383
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  • 3
    Electronic Resource
    Electronic Resource
    Microcalorimetric studies of the adsorption of N-ethylpyrrolidone, oligomeric and polymeric vinylpyrrolidone from CHCl3 on silica (1985)
    Springer
    Colloid & polymer science 263 (1985), S. 381-387 
    Details
    ISSN: 1435-1536
    Keywords: polymer adsorption ; microcalorimetry ; enthalpy of adsorption ; fraction of adhered segments ; silica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Adsorption enthalpies of N-ethylpyrrolidone, oligomeric and polymeric vinylpyrrolidone from CHCl3 solution on silica interfaces (Aerosil 200) have been measured at 25 °C by microcalorimetry. The dependence on surface coverage has been examined using measured adsorption isotherms. Binding enthalpies are calculated with fractions of adhered segments obtained by IR spectrometry and wetting enthalpies received from calorimetry. Fractions of adhered segments are derived from the adsorption enthalpies assuming equal binding enthalpies of monomers and polymer segments and considering desorption enthalpies of the solvent depending on the surface area of the polymer segments. The resulting enthalpies are compared with literature values from other solvents and the incongruity of the fraction of adhered segments obtained by IR, ESR, NMR and microcalorimetry is explained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01410384
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  • 4
    Electronic Resource
    Electronic Resource
    Tracer permeability of rat cortical blood vessels during regional hypothermia (1987)
    Springer
    Acta neuropathologica 73 (1987), S. 349-356 
    Details
    ISSN: 1432-0533
    Keywords: Blood-brain barrier ; Cortex ; Lanthanum tracer ; Potassium ; Ion-selective micro-electrode
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using lanthanum nitrate as a tracer, the permeability of rat cortical blood vessels was investigated during regional hypothermia of the cerebral cortex by electron microscopy. The concentration of K+ ions in the extracellular space of the cortex was determined using an ion-selective micro-electrode. Only at temperatures below about 7°C was an extravasation of tracer observed in a number of cortical capillaries and arterioles, where some of the tight junctions became widened and permeable. In a few cases penetration of the tracer into vacuoles of phagocytosing pericytes or macrophages was found. The extravasation of the tracer could mainly be observed in vessels on and below the cortical surface, less often in deeper cortical parts. At the same degree of hypothermia, the K+ concentration in the extracellular space increased, reaching a maximal value of ca. 6 mmol/l at a depth of about 200 μm. As could be shown in one previous experiment, the K+ concentration reached normal values again after rewarming to 37°C for 15 min, but an extravasation of the tracer was still observed. The role of the endothelial tight junctions and of the astrocytes together with the pericytes (or macrophages) is discussed regarding their importance for the effectiveness of the blood-brain barrier system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688258
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  • 5
    Electronic Resource
    Electronic Resource
    Besprechungen (1935)
    Springer
    Naturwissenschaften 23 (1935), S. 132-135 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01496971
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  • 6
    Electronic Resource
    Electronic Resource
    Besprechungen (1936)
    Springer
    Naturwissenschaften 24 (1936), S. 714-718 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01491563
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  • 7
    Electronic Resource
    Electronic Resource
    The cruciate ligaments in knee replacement (1989)
    Springer
    International orthopaedics 13 (1989), S. 13-16 
    Details
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Les ligaments croisés du genou ont été réséqués avec leurs insertions osseuses au cours d'arthroplasties totales du genou réalisées sur douze sujets atteints de graves lésions d'arthrose ou de polyarthrite rhumatoïde. Les ligaments ont été étudiés sur le plan histologique et biomécanique, et dix spécimens provenant d'adultes sains ont été utilisés comme contrôle. On a mis en évidence des différences significatives de la résistance et des propriétés visco-élastiques des ligaments entre ceux des patients arthrosiques et les autres. Les ligaments des genoux rhumatoïdes présentaient une résistance nettement plus faible comparativement à ceux des genoux arthrosiques. L'arthroplastie totale du genou qui remplace également la fonction ligamentaire, peut donc être efficace dans les genoux rhumatoïdes, grandement endommagés.
    Notes: Summary The cruciate ligaments were resected with their bony insertions during total knee arthroplasty carried out in 12 patients with severe rheumatoid or osteoarthritis. The ligaments were examined histologically and biomechanically, using ten specimens from healthy adults as a control. A significant difference was found in the tensile stiffness and viscoelastic properties of the ligaments between the arthritic and the control group. The ligaments in the rheumatoid knees had a distinctly inferior tensile strength compared with the osteoarthritic knees. Total knee replacement, which also replaces ligament function, should therefore be considered in severely damaged rheumatoid knees.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266716
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  • 8
    Electronic Resource
    Electronic Resource
    Protein transport to the epididymal lumen (1987)
    Springer
    Cell & tissue research 248 (1987), S. 527-530 
    Details
    ISSN: 1432-0878
    Keywords: Epididymal ultrastructure ; Peroxidase ; Protein transport ; Fluid-phase endocytosis ; Sprague-Dawley rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Experiments were performed to clarify the debate over the entry of circulating proteins into the epididymal lumen by use of the marker horseradish peroxidase (HRP). Epididymal tubules from the caput epididymidis of the rat were immersed in medium TC 199 containing HRP (3.5 mg/ ml) for 5 min to 3 h at 33° C. Sections were examined for the presence of tracer within the epithelial cells by electron microscopy. From 5 min to 3 h, vesicles containing peroxidase reaction products were found throughout the cytoplasm of the principal cells. Vesicles occurred close to both the basal and apical membranes, and many were found opening into the interstitial space and lumen, depending on the length of incubation. By 5 min labelled vesicles were infrequently found in the apical part of the cells. Reaction product was observed in the epididymal lumen adhering to the microvilli from 30 min of incubation onwards. At all periods of incubation peroxidase was present at the base of the epithelium and between the cells, but it was never found within the tight junctional complexes, and no reaction deposits were found within epithelial cells of tubules incubated in the absence of peroxidase. It is concluded that large molecules leaving the capillaries may enter the epididymal lumen in the caput by means of fluid-phase endocytosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00216479
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  • 9
    Electronic Resource
    Electronic Resource
    Photoperiod-dependent changes in TSH-like immunoreactivity of cells in the hypophysial pars tuberalis of the Djungarian hamster, Phodopus sungorus (1988)
    Springer
    Cell & tissue research 251 (1988), S. 183-187 
    Details
    ISSN: 1432-0878
    Keywords: Photoperiod ; Pituitary gland, Pars tuberalis ; TSH ; Immunohistochemistry ; Phodopus sungorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Certain secretory cells in the hypophysial pars tuberalis of the Djungarian hamster display marked circannual structural alterations. The present investigation deals with the immunohistochemical properties of this cell group. A distinct TSH-like immunoreactivity was found in secretory cells of this type in the pars tuberalis of animals exposed to long photoperiods, whereas under short photoperiods the TSH-like immunoreactivity was nearly absent. In the pars distalis, the number and distribution of TSH-positive cells did not differ significantly between animals maintained under long and under short photoperiods. LH-and FSH-positive cells could not be detected in the pars tuberalis, but they are clearly present in the pars distalis of both groups of hamsters. Our immunocytochemical results suggest that photoperiodic stimuli influence the secretory activity of TSH-like immunoreactive cells in the pars tuberalis. A connection with the neuroendrocrine-thyroid axis is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00215463
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  • 10
    Electronic Resource
    Electronic Resource
    Pattern of compartmentation in human seminiferous tubules showing dislocation of spermatogonia (1989)
    Springer
    Cell & tissue research 256 (1989), S. 183-190 
    Details
    ISSN: 1432-0878
    Keywords: Testis ; Spermatogonia, dislocation ; Compartmentation, pattern ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pattern of compartmentation of the seminiferous epithelium was investigated, using a lanthanum tracer technique, in human testicular biopsies of adult infertile men (age 27 to 44 years), where dislocation of spermatogonia from the basal lamina occurred. Spermatogonia type A and B were found in a two-or three-layered arrangement, in aberrant locations throughout the seminiferous epithelium, and in intratubular positions associated with fragments of Sertoli cell cytoplasm. Tracer impregnation was found around spermatogonia in a multilayered arrangement, indicating the extension of the basal compartment in a luminal direction. Single spermatogonia within the second or third layer of the seminiferous epithelium were regularly found to be surrounded by tracer. The junctional complex between the lateral membranes of adjacent Sertoli cells was devoid of tight junctions. Tracer penetration around spermatogonia in a more luminal position was prevented by intact Sertoli cell junctional complexes; tracer was also absent from intraluminal located spermatogonia associated with cytoplasmic fragments of Sertoli cells. The luminal extension of the basal compartment associated with the dislocation of spermatogonia clearly differs from the pattern of compartmentation during the movement of primary spermatocytes within undisturbed epithelium. There is a strong incidence of elevated serum levels of folliclestimulating hormone (〉7 U/l), indicating a suppression of Sertoli cell function; this may be the cause for the dislocation of spermatogonia and the changes of compartmentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224733
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