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  • 1985-1989  (2)
  • Acetylcholine  (1)
  • Insulin receptor  (1)
  • 1
    Electronic Resource
    Electronic Resource
    5′-flanking DNA of the human insulin receptor gene and long terminal repeat of mouse mammary tumour virus bind to the same nuclear protein(s) (1989)
    Springer
    Diabetologia 32 (1989), S. 877-880 
    Details
    ISSN: 1432-0428
    Keywords: Insulin receptor ; glucocorticoid responsive element ; gel mobility shift analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The interaction of nuclear protein extracted from rat liver and 5′-flanking DNA of the human insulin receptor gene was investigated with the aid of gel mobility shift analysis. When 5′-flanking DNA (-1255/-1206 or -385/-345 base pairs) was incubated with nuclear protein, two or three 32P-DNA species (protein binding DNA fragment(s) and free DNA fragment) were detected. These bands did not disappear in spite of increasing amounts of synthetic poly(dI-dC), showing that nuclear protein binds specifically to 5′-flanking DNA of the insulin receptor gene. Increasing amounts of long terminal repeat of mouse mammary tumour virus resulted in a reciprocal decrease in nuclear protein binding to 5′-flanking DNA of insulin receptor gene. These results suggest that 5′-flanking DNA of insulin receptor gene binds to the same nuclear protein to which long terminal repeat of mouse mammary tumour binds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297453
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Acetylcholine-induced change in intracellular Cl− activity of the mouse lacrimal acinar cells (1985)
    Springer
    Pflügers Archiv 405 (1985), S. 108-111 
    Details
    ISSN: 1432-2013
    Keywords: Lacrimal gland ; Cl− activity ; Acetylcholine ; Cl− permeability ; Ca2+ ionophore
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using double-barreled Cl−-sensitive microelectrodes, intracellular Cl− activity (A Cl i ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA Cl i was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (V m) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedV m to −63 mV and decreasedA Cl i to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inV m andA Cl i to those induced by ACh. It was concluded that Cl− was actively accumulated in the acinar cells and, in the secretory phase, Cl− efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl− permeability across the cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584530
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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