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  • 23.20.Lv  (2)
  • Wheat  (2)
  • wheat  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Association of a DNA marker with Hessian fly resistance gene H9 in wheat (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 964-968 
    Details
    ISSN: 1432-2242
    Keywords: RAPD ; Wheat ; Hessian fly ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224525
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of RAPD markers linked to the gene PM 1 for resistance to powdery mildew in wheat (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 832-840 
    Details
    ISSN: 1432-2242
    Keywords: Key words Powdery mildew ; Wheat ; RAPD markers ; Related inbred lines ; Bulked segregant analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Powdery mildew caused by Blumeria graminis DC. f. sp. triticiÉm. Marchal is an important disease of wheat (Triticum aestivum L. em Thell). We report here the identification of three random amplified polymorphic DNA (RAPD) markers closely linked to a gene for resistance to B. graminis in wheat. RAPD-PCR (polymerase chain reaction) analysis was conducted using bulked segregant analysis of closely related lines developed from a segregating F5 family. The F5 family was derived from a cross between the susceptible cultivar Clark and the resistant line Zhengzhou 871124. Genetic analysis indicated that resistance of Zhengzhou 871124 to powdery mildew is conferred by the gene Pm1. After performing RAPD-PCR analysis with 1300 arbitrary 10-mer primers and agarose-gel electrophoresis, two RAPD markers, UBC320420 and UBC638550, were identified to be co-segregating with the disease resistance. No recombinants were observed between either of the RAPD markers and the gene for resistance to powdery mildew after analysis of 244 F2 plants. The third RAPD marker, OPF12650, was identified with denaturing gradient-gel electrophoresis (DGGE), and was determined to be 5.4±1.9 cM from the resistance gene. UBC320420 and UBC638550 were present in wheat powdery mildew differential lines carrying the gene Pm1, suggesting linkage between these markers and the Pm1 resistance gene. Co-segregation between Pm1 and the two markers UBC320420 and UBC638550 was confirmed in a segregating population derived from a cross with CI14114, the wheat differential line carrying Pm1. The method of deriving closely related lines from inbred families that are segregating for a trait of interest should find wide application in the identification of DNA markers linked to important plant genes. The RAPD marker UBC638550 was converted to a sequence tagged site (STS). RAPD markers tightly linked to target genes may facilitate selection and enable gene pyramiding for powdery mildew resistance in wheat breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050484
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  • 3
    Electronic Resource
    Electronic Resource
    Crossability and embryo rescue enhancement in wide crosses between wheat and three Agropyron species (1990)
    Springer
    Euphytica 49 (1990), S. 209-214 
    Details
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Agropyron spp. ; embryo rescue ; wide crosses ; crossability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soft winter wheat lines were crossed with Agropyron intermedium, A. elongatum and A. trichophorum using pollen from single plants of Agropyron spp. to pollinate wheat spikes. Not only species but also individual plants within varieties of Agropyron species differed in percent seed set with a wheat genotype. In two arrays of crosses between two phenotypically different plants of A. elongatum and nine wheat lines, one Agropyron plant gave higher seed set (overall=27.1%) than the other (overall=3.7%). The differences were significant in seven of the nine cross combinations. Results are consistent with the hypothesis that these two plants differ in their crossability as pollen parents with wheat, and suggest the possibility of occurrence of crossability genes in wheatgrasses. The success rate of hybrid embryo rescue was higher (87.5%) with cold treatment (4°C) than without cold treatment (75.0%) of excised embryos on culture media. Results underscore the significance of genotype of the alien species for crossing with low crossable wheats, and of the physical factors for improving embryo rescue in wide crosses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036290
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  • 4
    Electronic Resource
    Electronic Resource
    Wheat anther culture: effect of genotype and environmental conditions (1991)
    Springer
    Plant cell, tissue and organ culture 24 (1991), S. 233-236 
    Details
    ISSN: 1573-5044
    Keywords: anther culture ; cultivars ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-two cultivars and lines of winter and spring wheat (Triticum aestivum L.) were studied, most for the first time, for their anther culture response. The response was genotype dependent. Plants grown in the field gave higher callus induction frequency than those grown in the greenhouse and the controlled environment chamber. Donor plants grown in a season of low drought stress as compared to a season of severe drought stress resulted in a higher frequency of callus induction. Spherical microcalli were observed in two wheat genotypes in some of only those anthers that were placed with only one loculus in contact with the medium. Wheat lines that were more responsive to anther culture were identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033482
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  • 5
    Electronic Resource
    Electronic Resource
    Structure of theN=59 nucleus97Sr: coexistence of spherical and deformed states (1990)
    Springer
    The European physical journal 337 (1990), S. 149-159 
    Details
    ISSN: 1434-601X
    Keywords: 23.20.Lv ; 21.60.Ev ; 23.20.−g
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A band with a rotational pattern based on a state at 585.1 keV has been identified in theN=59 neutron-rich nucleus97Sr. Its properties lead to the [422] 3/2 Nilsson-orbital assignment for the band head. There is evidence for a second band with the head at 644.7 keV and the configuration [541] 3/2. Since the ground state and the lowest excited levels are spherical, shape coexistence is established for97Sr. A deformed nature of several levels at 500–600 keV results also from QRPA-model calculations. The structure of the low-lying spherical levels has been studied in the frame of the IBF model. The results of the present investigations lead to a better understanding of theN=59 isotones which constitute the link between the spherical and deformed nuclei atA∼100 as a species with shape coexistence but without any indications of particular softness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294286
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  • 6
    Electronic Resource
    Electronic Resource
    A new high-spin isomer in 39 97 Y58 (1986)
    Springer
    The European physical journal 323 (1986), S. 59-67 
    Details
    ISSN: 1434-601X
    Keywords: 25.85.Ec ; 23.20.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract An isomeric state at 3,523 keV excitation energy in97Y with a half-life of 144(10) ms has been discovered with the fission-product separator JOSEF. This isomer is depopulated through aγ transition of 162 keV. AnE3 multipolarity for this transition is consistent with the measured conversion coefficients ofα K =0.98(20) andα T =1.00(19). Subsequent electromagnetic transitions populate several new97Y levels which have high spins. For the isomer the three-quasiparticle configuration [πg 9/2⊗ν(h 11/2,g 7/2)]27/2− is proposed. TheE3 transition with a strength about 2 single-particle units is supposed to be of the typeh 11 2/+1 →d 5 2/−1 . These findings provide evidence for the rapid shape transition atA≃100 since they indicate shell-model character of 39 97 Y58 even at high excitation energies while the immediate neighbour 39 98 Y59 contains a rotational band based on a level at 495 keV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294555
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