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  • Brassica napus  (4)
  • Electron microscopy  (4)
  • GASTRIC EMPTYING  (4)
  • Physics  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Estimation of the phospholipid distribution in the human platelet plasma membrane based on the effect of phospholipase A"2 from Naja nigricollis
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 856 (1986), S. 244-258 
    Details
    ISSN: 0005-2736
    Keywords: (Human, N. nigricollis) ; Electron microscopy ; Phospholipase A"2 ; Phospholipid asymmetry ; Platelet membrane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(86)90034-9
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  • 2
    Electronic Resource
    Electronic Resource
    The aggregation of human platelet induced by ganodermic acid S
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 986 (1989), S. 151-160 
    Details
    ISSN: 0005-2736
    Keywords: (Human blood) ; Aggregation ; Electron microscopy ; Ganodermic acid S ; Phosphoinositide metabolism ; Platelet membrane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0005-2736(89)90285-X
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  • 3
    Electronic Resource
    Electronic Resource
    The mode of action of primary bile salts on human platelets
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 1146 (1993), S. 282-293 
    Details
    ISSN: 0005-2736
    Keywords: (Human blood) ; Aggregation ; Component solubility ; Electron microscopy ; Leakage ; Optical absorbance ; Platelet membrane ; Primary bile salt
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(93)90367-9
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  • 4
    Electronic Resource
    Electronic Resource
    Changes in both calcium pool size and morphology of human platelets incubated in various concentrations of calcium ion. Calcium-specific bleb formation on platelet-membrane surface
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 940 (1988), S. 105-120 
    Details
    ISSN: 0005-2736
    Keywords: (Human blood) ; Bleb formation ; Calcium ion flux ; Electron microscopy ; Platelet membrane
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(88)90014-4
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  • 5
    Electronic Resource
    Electronic Resource
    Fatty acid inheritance in microspore-derived Populations of spring rapeseed (Brassica napus L.) (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 465-469 
    Details
    ISSN: 1432-2242
    Keywords: Microspore-derived population ; Fatty acids ; Inheritance ; Brassica napus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of major fatty acids in seed triglycerides was studied in three homozygous microspore-derived populations of spring rapeseed (Brassica napus L.). Crosses were made among parents with contrasting amounts of erucic, oleic, linoleic and linolenic acids. Microspores from F1 plants were cultured, and haploid plants were colchicine-doubled to provide homozygous populations reflecting F1 gametic arrays for fatty acid genotypes. Segregation ratios of the gametic arrays for specific fatty acid contents were compared to hypothetical models by the Chi-square test. Segregation pattern confirmed that erucic acid levels were controlled by two major loci, each having two alleles with additive effects. Oleic acid segregation indicated control of accumulation by at least two segregating genetic systems, one acting on chain elongation and the other involving desaturation. Accumulations of erucic acid and oleic acid were influenced by the same two loci, which control the chain elongation steps leading from oleic acid to erucic acid. Oleic acid was further influenced by at least two additional segregating loci involved in control of desaturation of oleic acid to form linoleic acid. Segregating alleles at loci involved in desaturation had a much smaller influence on oleic acid content than alleles segregating at loci controlling, the elongation of oleic acid to erucic acid. In a population free of erucic acid, the segregation pattern of linoleic acid levels fit a model involving segregating alleles at two loci. In contrast, segregation for linolenic acid content fits a three-locus additive model. In this study, microspore culture technology provided a rapid method of defining F1 gametic segregation for inheritance analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226746
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  • 6
    Electronic Resource
    Electronic Resource
    A comparison of traditional and haploid-derived breeding populations of oilseed rape (Brassica napus L.) for fatty acid composition of the seed oil (1990)
    Springer
    Euphytica 51 (1990), S. 59-65 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; oilseed rape ; doubled haploids ; fatty acids ; microspore-derived population ; single seed descent
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microspore embryogenesis technology allows plant breeders to efficiently generate homozygous micros-pore-derived breeding populations of oilseed rape (Brassica napus L.) without traditional generations of inbreeding. This study was conducted to compare the frequency distribution of microspore-derived population and single seed descent populations with respect to fatty acids of seed oil. Both microspore-derived populations and single seed descent populations were produced from each of three crosses made between selected parents containing contrasting amount of erucic, oleic, linoleic and linolenic acids. The fatty acid content of F3 plants derived lines (F5 seed) developed by single seed descent was compared to that of microspore-derived populations. The means, ranges and distribution pattern of seed fatty acid contents were similar in both populations for each fatty acid studied, although a few heterozygous lines were observed in the single seed descent populations. The results indicated that microspore-derived population form random, homozygous F1 plant derived gametic arrays for all fatty acids evaluated. Selection for altered fatty acid composition in microspore-derived and single seed descent homozygous populations should be equally efficient, in the absence of linkage of traits investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022893
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  • 7
    Electronic Resource
    Electronic Resource
    Evaluation of microspore-derived embryos as models for studying lipid biosynthesis in seed of rapessed (Brassica napus L.) (1991)
    Springer
    Euphytica 58 (1991), S. 145-155 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; rapeseed fatty acid accumulation ; microspore-derived embryo ; zygotic seed ; triacylglyceride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Microspore culture of rapeseed (Brassica napus L.) has provided a powerful tool not only for breeding but also in developmental studies. In this study, microspore-derived embryos (MDE) of B. napus were evaluated as a model in seed for studying accumulations of triacylglyceride (TAG) fatty acids in both a low and high erucic acid rapeseed line; and accumulations of TAG and free fatty acids (FFA) in a high erucic acid rapessed line. The accumulation patterns confirmed that MDE had a similar TAG fatty acid profile to seed during the embryo development within each genotype. The oil accumulation in MDE after 36 days in culture (DIC) approached levels similar to those in zygotic seed 25 days after flowering (DAF). Significant differences were detected in contents of both total free fatty acids and specific free fatty acids between MDE and seed. During the developmental period, total free fatty acids changed from 16% to 2.1% in MDE, but from 10.5% to 0.1% in seed. MDE had much higher percentage of free linolenic and erucic acids than seed, particularly during the late developmental stages. The current study indicated that MDE can be used as a model to study TAG and TAG fatty acids in seed but caution must be taken to study free fatty acid metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00022815
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  • 8
    Electronic Resource
    Electronic Resource
    Gastric Myoelectrical Activity in Healthy Children and Children with Functional Dyspepsia (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 2384-2391 
    Details
    ISSN: 1573-2568
    Keywords: ELECTROGASTROGRAPHY ; FUNCTIONAL DYSPEPSIA ; GASTROINTESTINAL MOTILITY ; GASTRIC EMPTYING
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this study was to investigateabnormalities in pediatric patients with functionaldyspepsia. Fifteen symptomatic pediatric patientsdiagnosed with functional dyspepsia and 17 aged-matchedhealthy controls were studied. Gastric myoelectricalactivity was recorded using surface electrogastrographyfor 1 hr in the fasting state and 1 hr after a testmeal. It was found that, in comparison with thecontrols, the children with functional dyspepsia had alower percentage of 2- to 4-cpm slow waves in bothfasting state (66.0 ± 4.7% vs 79.7 ± 3.1%,P 〈 0.07) and fed state (72.4 ± 5.4% vs 85.0± 2.9%, P 〈 0.04), and a significantly higherinstability of the dominant frequency in both fastingstate (0.50 ± 0.05 vs 0.31 ± 0.04, P 〈0.01) and fed state (0.39 ± 0.05 vs 0.25 ±0.03, P 〈 0.05). It was also found the postprandial increase inEGG dominant power in the patients was inverselycorrelated with the total symptom score (r = 0.63, P =0.03). It was concluded that abnormal gastricmyoelectrical activity may play a role in the pathogenesis ofpediatric functional dyspepsia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026661627304
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  • 9
    Electronic Resource
    Electronic Resource
    Efficiency and Efficacy of the Electrogastrogram (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 1023-1030 
    Details
    ISSN: 1573-2568
    Keywords: ELECTROGASTROGRAPHY ; GASTROINTESTINAL MOTILITY ; GASTRIC EMPTYING ; STOMACH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The efficiency and efficacy of theelectrogastrogram (EGG) involve a few practical factors,including recording length, sample size, and thecharacteristics of subjects. The aim of this study wasto investigate the effect of these factors on the accuracy ofEGG analysis. Gastric myoelectrical activity wasrecorded using electrogastrography in 24 subjects (ages22-91 years) for 1 hr in the fasting state and 2 hr after a test meal. Computerized spectralanalysis was performed to compute EGG parameters,including dominant frequency, dominant power, and thepercentage of 2-4 cycles per minute (cpm) slow waves. A parameter called misinterpretation was definedto investigate the effect of recording length. Theresults were as follows: (1) Using the recording lengthof 1 hr in each state as a gold standard, themisinterpretation for the recording length of 30 min was 27% forthe dominant frequency and 17% for the dominant power.When the recording length was reduced to 15 min, themisinterpretation increased to 61% for the dominant frequency and 38% for the dominant power. (2)With a sample size of 10 subjects and a recording lengthof 60 min, a statistically significant postprandialincrease was observed in the dominant frequency and power, and a trend in the postprandialincrease of the regularity of the EGG was noted. Whenthe sample size increased to 24 subjects, a significantpostprandial increase was found in all these parameters. (3) None of the EGG parameters exhibited anysignificant difference between the younger and oldersubjects or between men and women. In conclusion, arecording length of 30-60 min seems to be appropriate and produces reliable and predictable results.Age and gender do not affect any of the EGGparameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018830801785
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  • 10
    Electronic Resource
    Electronic Resource
    Impaired Postprandial Gastric Slow Waves in Patients with Functional Dyspepsia (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 1678-1684 
    Details
    ISSN: 1573-2568
    Keywords: ELECTROGASTROGRAPHY ; GASTROINTESTINAL MOTILITY ; FUNCTIONAL DYSPEPSIA ; GASTRIC EMPTYING
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this study was to investigate gastricmyoelectrical activity in patients with functionaldyspepsia. Thirteen healthy subjects and 14 patientswith functional dyspepsia participated in the study. The electrogastrogram (EGG) recording was madein each subject for 30 min in the fasting state and 120min after a standard test meal of 475 calories. Spectralanalysis methods were applied to derive quantitative EGG parameters. There was no difference in theEGG between the patients and controls in the fastingstate. However, abnormalities in the postprandial EGGwere found in the patients. The percentage of 2-4 cpmwaves was significantly lower (74.4 ± 4.0% vs85.7 ± 1.6%, P 〈 0.03) and the postprandialincrease in EGG dominant power was significantly less(–0.52 ± 0.92 dB vs 2.24 ± 0.88 dB,P 〈 0.03) in patients than in controls. It was alsofound that the percentage of postprandial 2-4 cpm wavescould be used to differentiate the patients withfunctional dyspepsia from the healthy controls with a specificity of 100% and a sensitivity of 43%. It was concluded that a subset of patients withfunctional dyspepsia have impaired gastric myoelectricalactivity in the fed state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018806913907
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