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  • radioimmunoassay  (5)
  • Cell & Developmental Biology  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Occurrence, distribution and nature of neuropeptide Y in the rat pancreas (1985)
    Springer
    Cellular and molecular life sciences 41 (1985), S. 1554-1557 
    Details
    ISSN: 1420-9071
    Keywords: Immunocytochemistry ; neuropeptide Y ; radioimmunoassay ; rat pancreas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Significant quantities of a newly discovered peptide, neuropeptide Y, were found in the rat pancreas, where they were localized to nerves in the exocrine parenchyma and around arterial and ductal structures. Although unaffected by surgical parasympathectomy, the periarterial and periductal nerves were abolished by chemical sympathectomy, suggesting that NPY is partially costored with sympathetic transmitters in nerve fibers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01964804
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  • 2
    Electronic Resource
    Electronic Resource
    Introduction (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 723-724 
    Details
    ISSN: 1420-9071
    Keywords: Autonomic/sensory nerves ; chromatography ; endocrine cells ; enzymes ; immunocytochemistry ; neuropeptides ; neurotransmitters ; processing of peptides ; radioimmunoassay ; regulatory peptides ; quantification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01945348
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  • 3
    Electronic Resource
    Electronic Resource
    The location of VIP in the pancreas of man and rat (1980)
    Springer
    Diabetologia 18 (1980), S. 73-78 
    Details
    ISSN: 1432-0428
    Keywords: Immunocytochemistry ; radioimmunoassay ; pancreas ; gut hormones ; vasoactive intestinal polypeptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary VIP has powerful stimulatory effects on both endocrine and exocrine pancreas but its localisation within the gland has not been established. In this study, human pancreas was obtained fresh at surgery (eleven) or within four hours of death (seven). The pancreas was also removed from rats (twenty-two). Immunocytochemical staining showed VIP to be present in fine nerve fibres in all areas of the pancreas. Many fibres were seen in the exocrine pancreas, running between the acini, and around ducts and blood vessels. In addition, dense networks of fibres were observed forming meshes around islets and occasional ganglia were found containing immunoreactive cell bodies. In general, there were fewer VIP fibres in the rat pancreas than in the human, but overall distribution was identical. The mean VIP content of whole human pancreatic tissue was 42±10 pmol/g wet weight (38±9 pmol/g in head, 49±6 pmol/g in body and 42±11 pmol/g in tail). Whole rat pancreatic tissue contained 28±7 pmol/g wet weight while preparations of isolated islets were found to contain 374±30 pmol/g. It is possible that the heavy VIP innervation of the islets described here indicates a role in the regulation of islet hormone release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01228307
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  • 4
    Electronic Resource
    Electronic Resource
    Elevated levels of vasoactive intestinal peptide in the eye and urinary bladder of diabetic and prediabetic Chinese hamsters (1985)
    Springer
    Diabetologia 28 (1985), S. 302-307 
    Details
    ISSN: 1432-0428
    Keywords: VIP ; radioimmunoassay ; immunocytochemistry ; eyes ; urinary bladder ; prediabetes ; diabetic Chinese hamsters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The eyes and urinary bladder of non-diabetic, prediabetic and diabetic Chinese hamsters were evaluated by radioimmunoassay and immunocytochemistry to determine the content and distribution of vasoactive intestinal peptide (VIP). The average concentration of VIP was increased in the eyes of all diabetic (pmol/g = 68%, pmol/organ = 50%) and prediabetic (pmol/g = 152%, pmol/organ = 115%) hamsters compared with age-matched non-diabetic animals. Immunocytochemistry showed that the elevation of VIP was primarily related to greater intensity of fluorescence of the nerve fibres in the vasculature of the choroid. The average content of VIP in the urinary bladder was greater in diabetic animals only on the basis of pmol/organ (135%) and in prediabetics on the basis of pmol/g (87%) compared with non-diabetic animals. Qualitative immunocytochemistry suggested that the elevated level of VIP was related to a larger distribution of nerve fibres in the urinary bladder of diabetic hamsters. The high level of VIP in the eyes and urinary bladder of diabetic and prediabetic hamsters is an interesting observation which should receive further study to determine whether it is an aetiological agent underlying the pathogenesis of ophthalmic complications and neurogenic bladder or the result of some pathological process which affects these organs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271690
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  • 5
    Electronic Resource
    Electronic Resource
    Distribution of a novel pituitary protein (7B2) in mammalian gastrointestinal tract and pancreas (1988)
    Springer
    Digestive diseases and sciences 33 (1988), S. 718-723 
    Details
    ISSN: 1573-2568
    Keywords: gastrointestinal tract ; pancreas ; 7B2 ; radioimmunoassay ; gel chromatography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The distribution of a novel pituitary protein (7B2) was determined in the gastrointestinal tract and pancreas of four mammalian species (man, pig, guinea pig, and rat) by a specific radioimmunoassay. The highest concentrations of cross-reacting immunoreactive 7B2 (IR-7B2) were observed in the pancreas and the proximal gut (antrum or duodenum). While the intestinal concentrations varied widely among species, pancreatic IR-7B2 concentrations appeared to be similar in all four species. In the rat, pancreatic islets were found to contain high concentrations of IR-7B2 (5.73± 0.14 fmol/islet, mean± sem). Neonatal capsaicin treatment and enteric nerve section did not affect the concentrations of IR-7B2 in the rat intestine. Layer separation of human gut showed that IR-7B2 is mainly (71± 8%) present in the epithelial fraction. Chromatographic analysis of intestinal and pancreatic extracts from the four species on Sephadex G-100 showed the presence of two immunoreactive peaks at Kav 0.3 and 0.6, but there were both inter- and intraspecies variations in the proportions of the larger and smaller molecular forms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01540436
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  • 6
    Electronic Resource
    Electronic Resource
    Lung endocrine cell markers, peptides, and amines (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 236 (1993), S. 169-175 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092360120
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  • 7
    Electronic Resource
    Electronic Resource
    The innervation of human teeth and gingival epithelium as revealed by means of an antiserum for protein gene product 9.5 (PGP 9.5) (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 189 (1990), S. 146-154 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The innervation of human teeth and oral mucosa has been studied in the past by different methods, none of which offered a clear description of the precise morphology of nerve fibers and terminals and of nerve organization as a whole. Recently, interesting findings have been obtained by means of immunohistochemical investigations for neurofilaments and S-100 proteins. A new brain-specific molecule, protein gene product 9.5 (PGP 9.5), has been used for the first time in the present research to investigate the distribution of nerves in human oral mucosa and decalcified teeth, about which there is a paucity of information. The data provided in this study, confirming previous work in other species, may be of value for understanding the anatomy of human oral innervation. In the oral mucosa, the antiserum labels nerve fibers, corpuscles, and neuroendocrine (Merkel) cells. In sections of decalcified teeth, numerous PGP 9.5 positive fibers are demonstrated in the pulp and in the inner 100 μm of dentin. The novel nerve tissue protein used, PGP 9.5, thus appears to be a reliable marker for studies of nerve fibers in human tissues and not to be affected by decalcification procedures. It could then be used for investigations on the innervation of normal and pathological calcified human tissues.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001890205
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  • 8
    Electronic Resource
    Electronic Resource
    An immunocytochemical study of cutaneous innervation and the distribution of neuropeptides and protein gene product 9.5 in man and commonly employed laboratory animals (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    American Journal of Anatomy 191 (1991), S. 369-383 
    Details
    ISSN: 0002-9106
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The cutaneous nerves of rat, cat, guinea pig, pig, and man were studied by immunocytochemistry to compare the staining potency of general neural markers and to investigate the density of nerves containing peptides. Antiserum to protein gene product 9.5 (PGP 9.5) stained more nerves than antisera to neurofilaments, neuronspecific enolase (NSE), and synaptophysin or histochemistry for acetylcholinesterase (AChE). Peptidergic axons showed species variation in density of distribution and were most abundant in pig and fewest in man. However, the specific peptides in nerves innervating the various structures were consistent between species. Nerve fibers immunoreactive for calcitonin gene-related peptide (CGRP) and/or vasoactive intestinal polypeptide (VIP) predominated in all the species; those immunoreactive to tachykinins (substance P and neurokinin A [NKA]) and neuropeptide tyrosine (NPY) were less abundant. Neonatal capsaicin, at the doses employed in this study, destroyed approximately 70% of CGRP- and tachykinin-immunoreactive sensory axons; whereas 6-hydroxydopamine (6-OHDA) at the doses employed resulted in a complete loss of NPY and tyrosine hydroxylase (TH) immunoreactivity without affecting VIP, CGRP, and tachykinins.Thus, this study confirms that antiserum to PGP 9.5 is the most suitable and practical marker for the demonstration of cutaneous nerves. Species differences exist in the density of peptidergic innervation, but apparently not for specific peptides. Not all sensory axons immunoreactive for CGRP and substance P/NKA are capsaicin-sensitive. However, all sympathetic TH- and NPY- immunoreactive axons are totally responsive to 6-OHDA; but no change was seen in VIP-immunoreactive axons, suggesting some demarcation of cutaneous adrenergic and cholinergic sympathetic fibers.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aja.1001910404
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