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Digitale Medien

Complex relation between triazine-susceptible phenotype and genotype in the weed Senecio vulgaris may be caused by chloroplast DNA polymorphism (1999)

Frey, J. E. ; Müller-Schärer, H. ; Frey, B. ; [weitere]

Springer

Theoretical and applied genetics 99 (1999), S. 578-586

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ISSN: 1432-2242

Schlagwort(e): Key words CpDNA polymorphism ; Heteroplasmy ; psbA gene ; Triazine resistance ; Senecio vulgaris

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract The weed Senecio vulgaris acquired high levels of resistance to triazine herbicides soon after the latter’s introduction. As in most weeds, triazine resistance is conferred by a point mutation in the chloroplast psbA gene that negatively affects the fitness of its carrier. To assess levels of triazine resistance in S. vulgaris field populations, we adopted a PCR-RFLP-based molecular diagnostic test recently developed for the triazine resistance-conferring region of the psbA gene of other weeds, including Brassica napus, Chenopodium spp. and Amaranthus spp., and compared these molecular results to the phenotypic response after triazine application. A highly significant linear correlation was found between phytotoxic symptoms and biomass reduction. Variability in phenotypic response was not only found between populations or inbred lines of S. vulgaris but also within replicates of the same inbred line. No clear relationship, however, was found between the DNA restriction pattern and the phenotypic response to triazine application, thereby throwing doubt on the use of such molecular diagnostic tests to track triazine resistance in S. vulgaris. Our results indicate that the chloroplast genome of S. vulgaris is polymorphic and that the level of polymorphism may be variable within single leaves of individual plants. We discuss the possible genetic basis of this polymorphism and its consequence for the acquisition and inheritance of chloroplast-based traits.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s001220051271

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Digitale Medien

Gadolinium enhancement: improved MRI detection of retinoblastoma extension into the optic nerve (1996)

Ainbinder, D. J. ; Haik, B. G. ; Frei, D. F. ; [weitere]

Springer

Neuroradiology 38 (1996), S. 778-781

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ISSN: 1432-1920

Schlagwort(e): Key words Retinoblastoma ; Optic nerve ; Magnetic resonance imaging

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract We performed T1-, T2-, proton density-weighted, and T1-weighted gadolinium-enhanced MRI on 24 patients with retinoblastoma, using a 1.5 T superconducting unit and head and orbital surface coil imaging. All patients underwent a complete ophthalmologic examination, including B-scan ultrasonography. CT was performed on 10 of 24 patients. Pathologic correlation was obtained in 18 patients who required enucleation. Contrast-enhanced T1-weighted MRI with fat suppression was the sequence most sensitive to optic nerve extension and provided the greatest differentiation between tumor and uninvolved extrascleral tissue. Retinoblastoma demonstrated contrast enhancement.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s002340050346

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Digitale Medien

Einflu\ von Absto\ungskrisen und immunsuppressiver Therapie auf die Lymphozytensubpopulationen von Patienten nach Nierentransplantation (1990)

Kiparski, A. ; Frei, D. ; Fierz, W. ; [weitere]

Springer

Journal of molecular medicine 68 (1990), S. 408-414

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ISSN: 1432-1440

Schlagwort(e): Renal transplantation ; Immunosuppression ; Cyclosporine A ; Immunologic monitoring ; T lymphocytes ; Cell surface antigens ; Monoclonal antibodies ; Flow cytometry

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Summary The lymphocyte subsets in the peripheral blood were examined 3 times a week in 17 patients receiving a cadaveric renal allograft using 2-color flow cytometry and several combinations of monoclonal antibodies. Patients who experienced a rejection crisis (n=12) had a significantly higher CD4/CD8-ratio (2.72±1.26 mean±SD) than patients with stable graft function (1.76±1.33, p〈0.05). 9/12 patients showed 0–3 days prior to the rejection episode an increase of the CD4/CD8-ratio (≥0.5) and/or a high ratio (≥2.5) with a decrease following antirejection therapy. The activation markers HLA-DR and IL-2 receptor on T cells were increased only during 3/12 rejection episodes. Patients with rejections resistant to prednisone pulse therapy (n=6) had significantly more lymphocytes/mm3 in the peripheral blood (1111.7±597.5) than successfully treated patients (n=6, 336.7±196.0, p〈0.02). Antirejection therapy with prednisone pulses and/or antithymocyte globuline resulted in a significant decrease of T lymphocytes (CD3+) with a selective reduction of T helper/inducer cells (CD4+). 6 months after renal transplantation the patients had a higher percentage of suppressor/cytotoxic cells (CD8+) compared to the pretransplant values (26.3±10.9% vs 17.7±6.2%, p〈0.02) and blood donors (16.3±6.2%, p〈0.01). Furthermore the percentage of T helper cells (CD4+/CD28−) was significantly higher and the T suppressor-inducer cells (CD4+/CD28+) were significantly lower compared to the controls. Serial flow cytometric determinations of lymphocyte subsets in renal allograft recipients may be helpful in some cases although rejection episodes could not be predicted in the individual patient.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01648582

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Digitale Medien

Akute Leukämie nach Nierentransplantation (1980)

Frei, D. ; Binswanger, U. ; Fehr, J. ; [weitere]

Springer

Annals of hematology 40 (1980), S. 343-348

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ISSN: 1432-0584

Schlagwort(e): Acute leukemia ; Immunosuppressive therapy ; Kidney allotransplantation ; Akute Leukämie ; Immunosuppression ; Nierenallotransplantation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Beschreibung / Inhaltsverzeichnis: Zusammenfassung In der Literatur sind vier Fälle mit Entwicklung einer akuten myeloischen und sechs Fälle mit Entwicklung einer chronischen myeloischen Leukämie unter Immunosuppression mit Azathioprin und Prednison bei nierentransplantierten Patienten beschrieben. Wir berichten über zwei weitere akute Leukämien während chronischer Immunosuppression bei Zustand nach Nierentransplantation. Der erste, 29jährige Patient entwickelte zehn Jahre nach erfolgreicher Nierentransplantation die Zeichen einer akuten lymphatischen Leukämie. Beim zweiten, 47jährigen Patienten wurden fünf Jahre nach erfolgreicher Nierentransplantation Hepatosplenomegalie und Pancytopenie beobachtet; nach Splenektomie wurde die Diagnose einer subakuten myeloischen Leukämie gestellt. Beide Patienten standen unter langdauernder immunosuppressiver Therapie (Azathioprin und Prednison),die in beiden Fällen nach der Diagnose einer Leukämie abgesetzt wurde. Ein Zusammenhang zwischen dem Auftreten der Leukämie und der chronischen Immunosuppression wird postuliert.

Notizen: Summary Four cases of acute myelogenous leukemia and six cases of chronic myelogenous leukemia after treatment with azathioprine and prednisone for renal allotransplantation have been described in the literature. We report another two cases of acute leukemia 10 and 5 years after successful renal allotransplantation. Patient 1, a 29-year-old farmer, exhibited the signs of acute lymphatic leukemia resistent to treatment with cytostatic agents. Death was due to pneumonia. Patient 2, a 47-year-old salesman, developed pancytopenia together with splenomegaly. After splenectomy an atypical subacute myeloid leukemia became apparent which was not treated due to withdrawal of the patient. He died 2 months after diagnosis. Both patients received long-term immunosuppressive therapy with azathiopine and prednisone until the leukemia was diagnosed. A relationship between long-term immunosuppression and the occurrence of leukemia is postulated.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF01025589

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