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  • 1
    Electronic Resource
    Electronic Resource
    Physiologic and metabolic results of pancreatic cold storage with Histidine-Tryptophan-Ketoglutarate-HTK solution (Custodiol)® in the porcine autotransplantation model (2000)
    Springer
    Transplant international 13 (2000), S. 98-105 
    Details
    ISSN: 1432-2277
    Keywords: Key words Pancreas preservation ; HTK-solution ; Segmental porcine pancreatic autotransplantation ; Delayed endocrine graft function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Delayed graft function (DEGF) remains an obscure phenomenon in organ transplantation. For the optimal washing of the compounds of the different organ flush solutions, adequate temperature and equilibrium of electrolytes have to be provided. A total of 29 landrace pigs weighing 37.3–5.4 kg were included in this study. According to the model, the left hemipancreas was perfused with Histidine-Tryptophan-Ketoglutarate (HTK)-solution and autotransplanted after 24 h (G1, n = 13) and 48 h (G2, n = 4) of cold storage (CS). Results were compared with grafts perfused with UW-solution and autotransplanted after 24 h (G3, n = 8) and 48 h (G4, n = 4) CS respectively. Daily measurements of glycemia, glucosuria, amylase and lipase were carried out. HTK perfusion resulted in an increase in wet weight of the grafts after 24 h and 48 h CS (P = 0.031 vs UW). Postoperative glycemia levels in pancreases flushed with HTK-solution were higher after 48 h than after 24 h CS until the 6th postoperative day, when the glycemia returned to normal range (P = 0.02), suggesting a delayed endocrine graft function. The mean IVGTT values attained after full function were comparable in G1 and in G3 (–1.22 ± 0.23 vs. –1.5 ± 0.65). The rises in serum amylase and lipase levels were more pronounced after 48 h CS in both HTK and UW groups, (P = n.s.). Appearance of interstitial and intracellular edema after CS and reperfusion did not influence the function.¶Conclusion: HTK-solution is suitable for 24 h pancreatic preservation in vivo; the perfusion requires at least 4 min for electrolyte equilibration. Long preservation time (48 h) resulted in a transitory DEGF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001470050669
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  • 2
    Electronic Resource
    Electronic Resource
    31P kernspinspektroskopie der magenwand nach proximaler selektiver vagotomie (1990)
    Springer
    Langenbeck's archives of surgery 375 (1990), S. 205-207 
    Details
    ISSN: 1435-2451
    Keywords: Proximal selective vagotomy ; Metabolism of the gastric wall ; 31P-NMR spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Im Experiment wurde der Einfluß der SPV auf den Phosphatmetabolismus der Magenwand bei Ratten mittels 31P-NMR Spektroskopie untersucht. Die SPV bewirkt unmittelbar nach der Operation eine Destruktion des Stoffwechsels der Magenwandzellen. Vier Tage nch der SPV kommt es zur Regeneration des Metabolismus. Eine Woche nach der Operation ist die funktionelle Zellregeneration abgeschlossen, der Stoffwechsel aber verlangsamt. Dies hat eine reduzierte Produktion von S`aure und Schutzfaktoren der Magenschleimhaut zur Folge. Experimentelle Läasionen der Magenschleimhaut durch Phenylbutazon bewirken ebenso eine Zerstörung des Stoffwechsels der Magenwandzellen bis zum kompletten Zelluntergang.
    Notes: Summary The phosphate metabolism of the gastric wall after proximal selective vagotomy (PSV) was investigated by means of 31P-NMR spectroscopy. The destruction of metabolism has been found just after PSV resulting in a significant decrease of adenosine diphosphates and adenosine triphosphates. 4 and 7 days after PSV the progress of metabolism regeneration was detected, nevertheless with the retardation of high energy phosphates ischemic degradation. The results indicate not only a hydrochloric acid activity reduction but diminution of gastric mucosa protective factors, too. In experimental gastric ulcers no energy phosphates have been found suggesting a mucosa cells necrobiosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187439
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  • 3
    Electronic Resource
    Electronic Resource
    Die Beziehung zwischen implantierter Betazellmasse und metabolischer Stoffwechsellage nach Transplantation pankreatischer Fragmente beim Hund (1987)
    Springer
    Langenbeck's archives of surgery 370 (1987), S. 211-221 
    Details
    ISSN: 1435-2451
    Keywords: Islet transplantation ; Dogs ; Metabolic function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Pancreata von 20 Hunden wurden total excidiert, mittels einer intraductalen Kollagenaseperfusionstechnik und mechanischer Zerkleinerung zu insulinproduzierenden Pankreasfragmenten verarbeitet und als Autotransplantat in die Milz eines jeden Tieres mittels intravenösem Reflux in die V. lienalis injiziert. Bei 75% aller transplantierten Tiere wurde eine langfristige normoglykämische Stoffwechsellage hergestellt, wobei die Glucosetoleranzteste im Vergleich zu 13 Kontrolltieren pathologisch verändert waren. Der Milzinsulingehalt der erfolgreich transplantierten Tiere war auf ca. 5 bis 15% des urspünglichen Pankreasinsulingehaltes reduziert, was für die Funktionseinschränkung verantwortlich sein dürfte. Die angewandte intraductale Kollagenaseperfusionstechnik ist eine zuverlässige Methode zur Wiederherstellung einer normoglykämischen Stoffwechsellage nach totaler Pankreatektomie im Autotransplantationsmodell. Zur Steigerung der Funktionsfähigkeit des Inseltransplantates ist eine erhöhte Inselimplantationsrate erforderlich.
    Notes: Summary 20 dogs underwent total pancreatectomy. Pancreatic fragments were produced using an intraductal collagenase perfusion technique and mechanical disruption of the pancreatic gland. The resulting tissue suspension was transplanted as an autotransplant to the spleen of each animal by intravenous reflux injection into the splenic vein. In 75% of all transplanted animals long-term function with normoglycemia was restored while i.v. glucose tolerance tests were impaired as compared to 13 controls. The spleen insulin content of these sucessfully transplanted animals corresponded to only 5 to 15% of the original pancreas insulin content which was responsible for the reduced function. Intraductal collagenase perfusion is a reliable method to restore normoglycemia after total pancreatectomy in the autotransplant model using pancreatic fragments. To improve the metabolic function of the islet transplant an increased number of islets has to be implanted.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01259309
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    Paper (German National Licenses)
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