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  • Hyperacute xenograft rejection  (2)
  • Mamma carcinoma  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Prevention of hyperacute xenograft rejection in orthotopic xenotransplantation of pig hearts into baboons using immunoadsorption of antibodies and complement factors (2000)
    Springer
    Transplant international 13 (2000), S. S508 
    Details
    ISSN: 1432-2277
    Keywords: Key words Immunoadsorption ; Ig-Therasorb ; Hyperacute xenograft rejection ; Baboon ; Orthotopic xenotransplantation ; Xenoreactive antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To prevent hyperacute xenograft rejection (HXR) caused by preformed natural antibodies (XNAb) after orthotopic heart xenotransplantation (oXHTx) of landrace pig hearts into baboons, we used immunoadsorption of immunoglobulins IgG, IgM and IgA and complement with the reusable Ig-Therasorb column. In addition to functional data, tissue was sampled for histological, immunohistochemical and electron microscopical analysis. We performed three oXHTx of landrace pig hearts to baboons using extracorporeal circulation (ECC) connected to the immunoadsorption unit. Intraoperative treatment consisted of four cycles of immunoabsorption (IA). One oXHTx of a baboon without IA served as a control. A mismatch of donor and recipient heart size was prevented by selecting a 30–40 % lower body weight of donor pigs than recipients. Four cycles of IA removed more than 80 % of IgG, IgM and IgA, 86 % of anti-pig antibodies and 66 % of complement factors C3 and C4 from plasma. The graft of the control animal failed after 29 min. Orthotopic xenotransplantation with IA was selectively terminated after 100 min, 11 h and 21 h, respectively without any histological signs of HXR in light and electron microscopy. After weaning off from ECC these donor xenografts showed sufficient function with normal ECG and excellent cardiac output in echocardiography and invasive measurement (1.93 ± 0.035 l/min). The myocardium of the control xenograft demonstrated more deposits of Ig and complement components (C3, C4) than in the IA group. Baboons survive HXR after orthotopic pig heart xenotransplantation due to antibody depletion by reusable Ig-Therasorb column treatment. Long-term survival in an orthotopic baboon xenotransplantation model after IA, especially in combination with transgenic pig organs, could be a reliable preclinical trial for future clinical xenotransplantation programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001470050392
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of ischemic time on hyperacute xenograft rejection of pig hearts in a working heart perfusion model with human blood (2000)
    Springer
    Transplant international 13 (2000), S. S494 
    Details
    ISSN: 1432-2277
    Keywords: Key words Ischemic time ; Hyperacute xenograft rejection ; Xenotransplantation ; Ig-Therasorb column ; Immunoadsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In xenotransplantation long ischemic time of grafts is supposed to have a marked influence on hyperacute rejection (HXR). We investigated the influence of different cold ischemic times on HXR of ex vivo “working pig hearts” perfused with human blood. Xenoreactive natural antibodies (XNAb) as a trigger of HXR were eliminated by Ig-Therasorb immunoadsorption (IA). Explanted Landrace pig hearts of group G1 and group G3 (with additional IA) underwent 4 h of cold ischemia prior to xenoperfusion. Control groups G2 and G4 (with IA) were kept ischemic for only 46.6 ± 15.8 and 51.2 ± 4.2 min, respectively. Ischemic time prolonged the perfusion time in our working heart model (G1: 356 ± 46.1 min; G2: 125 ± 31 min; P 〈 0.05). IA had no additional impact on perfusion time but was effective by itself. The heart weight increased fourfold more in G2 as compared to the other groups. IA without ischemia significantly improved cardiac output in G4 (G3: 198.8 ± 15.4 mL/min; G4: 338.5 ± 16.0 mL/min). Coronary flow in G2 was significantly lower than in G1 (G1: 157.9 ± 9.15 mL/min; G2: 59.4 ± 20.1 mL/min). Histological signs of HXR (light and electron microscopy) could be found in G2 in contrast to the other groups. Parameters of serological damage showed a minimum in G4 and the maximum in G2. In G1 XNAb were nearly equally eliminated immediately after the start of xenoperfusion as in IA groups G4 and G3. Four hours of ischemic time showed beneficial effects in preventing HXR, possibly caused by changes of the endothelial cell surface (for example, glycosylation or loss of α1–3Gal epitopes with a hapten effect).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001470050390
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Seltene Differentialdiagnose bei einem Mammatumor Riesenzelltumor der Rippen (1997)
    Springer
    Langenbeck's archives of surgery 382 (1997), S. 64-68 
    Details
    ISSN: 1435-2451
    Keywords: Key words Osteoclastoma ; Mamma carcinoma ; Breast tumor ; Chest wall defect ; Schlüsselwörter Osteoklastom ; Mammakarzinom ; Brusttumor ; Thoraxwanddefekt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir berichten über 1 Patientin, welche unter dem Verdacht eines Mammakarzinoms biopsiert wurde, wobei ein äußerst seltener Riesenzelltumor der Rippen festgestellt wurde. Der semimaligne Knochentumor rezidiviert gerne. Die Klinik ist unspezifisch; die Erstdiagnose erfolgt zumeist spät. Als Differentialdiagnose kommen primäre und sekundäre Knochenerkrankungen und Tumoren der Thoraxorgane in Betracht. In unserem Fall wurde der Tumor unter Erhalt der Brust in toto mitsamt den Rippen reseziert und der Defekt mit einer Koriumplastik gedeckt. Es wird auf verschiedene Methoden zur Thoraxwanddefektdeckung eingegangen.
    Notes: Abstract We report a case of a breast tumor. As carcinoma of the breast was suspected, a biopsy was taken and a very rare osteoclastoma originating in the rib was identified. Semimalignant bone tumors tend to recur locally. The symptoms are nonspecific; the initial diagnosis is often made late. To differentiate the diagnosis, one should think about primary and secondary bone diseases and tumors of the organs of the thorax. In our case, the tumor was completely resected, including the ribs, and the defect was covered with a corium plasty. In this way, we are able to save the breast. We discuss different methods for covering chest wall defects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00008020
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Seltene differentialdiagnose bei einem Mammatumor Riesenzelltumor der Rippen (1997)
    Springer
    Langenbeck's archives of surgery 382 (1997), S. 64-68 
    Details
    ISSN: 1435-2451
    Keywords: Osteoclastoma ; Mamma carcinoma ; Breast tumor ; Chest wall defect ; Schlüsselwörter ; Osteoklastom ; Mammakarzinom ; Brusttumor ; Thoraxwanddefekt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir berichten über 1 Patientin, welche unter dem Verdacht eines Mammakarzinoms biopsiert wurde, wobei ein äußerst seltener Riesenzelltumor der Rippen festgestellt wurde. Der semimaligne Knochentumor rezidiviert gerne. Die Klinik ist unspezifisch; die Erstdiagnose erfolgt zumeist spät. Als Differentialdiagnose kommen primäre und sekundäre Knochenerkrankungen und Tumoren der Thoraxorgane in Betracht. In unserem Fall wurde der Tumor unter Erhalt der Brust in toto mitsamt den Rippen reseziert und der Defekt mit einer Koriumplastik gedeckt. Es wird auf verschiedene Methoden zur Thoraxwanddefektdeckung eingegangen.
    Notes: Abstract We report a case of a breast tumor. As carcinoma of the breast was suspected, a biopsy was taken and a very rare osteoclastoma originating in the rib was identified. Seminalignant bone tumors tend to recur locally. The symptoms are nonspecific; the initial diagnosis is often made late. To differentiate the diagnosis, one should think about primary and secondary bone diseases and tumors of the organs of the thorax. In our case, the tumor was completely resected, including the ribs, and the defect was covered with a corium plasty. In this way, we are able to save the breast. We discuss different methods for covering chest wall defects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02465090
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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