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  • Immunohistochemistry  (2)
  • Imaging procedures  (1)
  • Key words. Serotonin; ligand-gated receptor; G-protein-coupled receptor; patch-clamp; cerebral cortical neuron.  (1)
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Material
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Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Differential effects of the serotonin receptors on cultured rat cerebral cortical neurons (1997)
    Springer
    Cellular and molecular life sciences 53 (1997), S. 233-236 
    Details
    ISSN: 1420-9071
    Keywords: Key words. Serotonin; ligand-gated receptor; G-protein-coupled receptor; patch-clamp; cerebral cortical neuron.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Effects of serotonin (5-HT) on cerebral cortical neurons were examined by patch clamp techniques. 5-HT produced a variety of responses such as outward (19/73 patches/neurons), slow inward (15/73 patches/neurons), fast inward (8/73 patches/neurons), and mixed currents (initially fast inward deflection followed by an outward response: 2/73 patches/neurons), with a latency of 12 sec, 15 sec, 0 sec, and 0 sec respectively, at a holding potential of −60 mV in whole-cell patches. The fast inward currents were again evoked by a selective 5-HT3 receptor agonist, 1-(m-chlorophenyl)-biguanide hydrochloride (CPBG). In the cell-attached patch clamp configuration, 5-HT inside the patch pipette elicited single channel currents with slope conductances of 42 pS and 132 pS (4/42 patches/neurons). CPBG inside the patch pipette evoked inward single channel currents with a lower slope conductance of 41 pS (3/23 patches/neurons). In contrast, application of 5-HT or a 5-HT2 receptor agonist, α-methyl-5-hydroxytryptamine-maleate, outside the patch pipette induced outward single channel currents with a major slope conductance of 140 pS (8/30 patches/neurons) or 135 pS (6/20 patches/neurons), respectively. These results indicate that the outward and fast inward currents may be mediated respectively by the 5-HT2 receptor, which is coupled to a G-protein, and by the 5-HT3 receptor, which contains the non-selective cation channel, and that the mixed type may be caused by both the 5-HT2 and 5-HT3 receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00000596
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transferrin receptors in injured brain (1990)
    Springer
    Acta neuropathologica 79 (1990), S. 686-688 
    Details
    ISSN: 1432-0533
    Keywords: Transferrin receptor ; Brain injury ; Edema fluid ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recent studies have demonstrated the presence of transferrin receptors (Tf-R) in the central nervous system. The present study of Wistar rats with experimentally induced brain injuries, using immunohistochemistry at the light microscopy level, demonstrated the presence of Tf-R in regenerated endothelial cells, reactive astrocytes and in other cells, probably macrophages. Although Tf-R were seen in proliferating cells, Tf-R were also observed in nonproliferating cells, many of them macrophages. The receptors perhaps bind transferrin in edema fluid and play an important role in lesion repair.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294248
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cerebral endothelial regeneration following experimental brain injury (1989)
    Springer
    Acta neuropathologica 77 (1989), S. 397-401 
    Details
    ISSN: 1432-0533
    Keywords: Blood-brain barrier ; Bromodeoxyuridine ; Cerebral endothelium ; Immunohistochemistry ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary It is still unknown when and in which area endothelial regeneration occurs after brain injury, and to what extent such changes depend on the severity of the injury. We have, therefore, studied bromodeoxyuridine (BrdU) uptake by regenerating endothelial cells in two different groups of rats given cold lesions using immunohistochemistry employing anti-BrdU monoclonal antibody, anti-factor VIII-related antigen antibody and anti-glial fibrillary acidic protein antibody. The earliest evidence for the presence of BrdU-positive endothelial cells (BrdU+end) was observed at 2 days after injury, the injured endothelial cells regenerating from the edge toward the center of the lesion in both groups. We considered that edema fluid could act as an important factor, since at 2 days post-injury BrdU+end were not in contact with macrophages and were always found in Evans blue-stained areas. Study of endothelial cell kinetics also confirmed that the repair of injured endothelial cells was intimately involved in the reconstruction of the blood-brain barrier, since the time of disappearance of BrdU+end coincided with the disappearance of Evans blue-stained areas. The difference in the process of endothelial regeneration was first apparent on the 3rd day, there being no difference at 2 days.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00687374
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Laparoscopy preferable to imaging procedures in detecting metastases of a pancreas carcinoma to the liver (1994)
    Springer
    Surgical endoscopy and other interventional techniques 8 (1994), S. 1340-1342 
    Details
    ISSN: 1432-2218
    Keywords: Laparoscopy ; Imaging procedures ; Pancreas carcinoma ; Hepatic metastases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In two patients a pancreatic mass, 6 and 4 cm in diameter, respectively, was detected using imaging techniques. Computed tomography, ultrasound, and angiography revealed no evidence of a metastatic lesion in the liver. In the first patient, exploration was done prior to a possible radical operation. Multiple hepatic metastases forming thin, flat lesions on the surface of the liver were present and palliative surgery only was done. In the other patient, laparoscopy was done before surgical exploration and multiple hepatic metastases on the surface of the liver were clearly evident. Laparoscopy can help to avoid unnecessary explorations in patients with large pancreas cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188297
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    Paper (German National Licenses)
    Fulltext
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