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  • 1
    Digitale Medien
    Digitale Medien
    Isozyme variation and RFLPs at the β-amylase loci in wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 691-699 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Hexaploid wheat ; β-Amylase ; Isozymes ; RFLPs ; IEF
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a β-amylase probe using three restriction enzymes, and for mature grain β-amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain β-amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303514
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  • 2
    Digitale Medien
    Digitale Medien
    The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 342-348 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Hexaploid wheat ; RFLPs ; Homoeology ; Chromosome arm ; Alien additions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary To investigate the use of RFLP analysis in the Triticeae, a set of low copy number probes has been isolated from a wheat cDNA library. The probes identify each of the 14 homoeologous chromosome arms of wheat as determined by analysis of DNA fragments hybridizing to the probes in aneuploid lines of Chinese Spring. These probes can be used in RFLP analyses both for the assignment of homoeology of alien chromosomes or arms added to wheat, and for the determination of chromosome dosage in wheat aneuploids. Different chromosomes from various Triticeae species can therefore be followed in a wheat genetic background using a single technique. The potential uses of the set in facilitating the transfer of alien segments into wheat are outlined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265294
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular cloning and chromosomal location of genes encoding the “Early-methionine-labelled” (Em) polypeptide of Triticum aestivum L. var. Chinese Spring (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 43-48 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Em genes ; Group 1 chromosomes ; RFLPs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The “Early-methionine-labelled” (Em) polypeptide is the most abundant cytosolic polypeptide found in mature wheat embryos. Using a near full-length cDNA clone as a hybridisation probe to detect genomic sequences by Southern blotting of electrophoretic separations of genomic DNA derived from Triticum aestivum L. var. Chinese Spring and a series of its aneuploid derivatives, we demonstrate that the Em polypeptide is the product of a small multigene family in which the copies are located on each of the long arms of the homoeologous group 1 chromosomes. Screening of a variety of genotypes additionally reveals a number of restriction fragment length polymorphisms associated with these loci. Screening of a library of genomic DNA cloned in the vector λEMBL 4 has resulted in the isolation of a genomic fragment containing two closely linked Em genes. These are separated by ca. 2.5 kb. Analysis of restriction enzyme digests of this clones fragment has identified it as originating from chromosome 1A.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224014
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  • 4
    Digitale Medien
    Digitale Medien
    RFLP-based genetic maps of wheat homoeologous group 7 chromosomes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 495-504 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Genetic mapping ; RFLPs ; Isozymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Restriction fragment length polymorphism (RFLP) mapping was attempted using 18 cDNA clones, 14 anonymous and 4 of known function, which had been shown to have homologous DNA sequences on the group 7 chromosomes of wheat. The loci identified by these probes have been mapped on one or more chromosomes in this homoeologous group using linkage data derived from various F2, random inbred, doubled haploid and single chromosome recombinant populations. The maps also include three isozyme loci, five disease resistance loci, two anthocyanin pigment loci and a vernalisation response locus. The mapping data have been used to determine the extent of map co-linearity over the A, B and D genomes, the degree of RFLP variability in the three genomes and the relative efficiency of various restriction enzymes in detecting RFLPs in wheat. The strategy for future mapping in wheat, particularly the use of “alien” genomes or segments, such as that from Aegilops ventricosa used here, is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00290833
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  • 5
    Digitale Medien
    Digitale Medien
    Location of β-amylase sequences in wheat and its relatives (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 286-290 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Wheat ; Aegilops ; Rye ; β-Amylase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtained from wheat aneuploid and wheat-alien addition lines. Structural genes were identified on homoeologous group 4 and 5 chromosomes, confirming the results of isozyme studies. In addition, a further set of structural genes was found on homoeologous group 2 chromosomes. It is proposed that there are two homoeoallelic series, β-Amy-1 on group 4 or 5 chromosomes, and β-Amy-2 on group 2 chromosomes. Evidence is presented that each locus contains one or two β-amylase structural genes, and it is suggested that the large number of isozymes seen upon IEF are due to post-translational modifications.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303966
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  • 6
    Digitale Medien
    Digitale Medien
    The localisation of LH-RH neurones in the diencephalon of the domestic hen (1982)
    Springer
    Cell & tissue research 222 (1982), S. 283-298 
    Details
    ISSN: 1432-0878
    Schlagwort(e): LH-RH-neurones ; Diencephalon ; Immunohistochemistry ; Chicken
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Nerve fibres and perikarya containing LH-RH-like material were identified immunohistochemically in the diencephalon of the domestic hen using the peroxidase-anti-peroxidase technique. Perikarya were thinly scattered in bilateral bands close to the third ventricle extending from the nucleus praeopticus paraventricularis magnocellularis, passing in front of the anterior commissure into the septal area. In this latter area, the perikarya tended to spread out laterally. A few perikarya were seen in the anterior portion of the nucleus paraventricularis magnocellularis but were not found in the infundibular nuclear complex. Fibre tracts were seen running dorso-ventrally in the preoptic area apparently associated with the lamina terminalis. Fibres, possibly nerve terminals, were found in the lamina terminalis and in the external layers of the anterior and posterior divisions of the median eminence. A large number of fibres was seen distributed throughout the infundibular nuclear complex; scattered fibres were found close to the third ventricle in the anterior hypothalamus. Extrahypothalamic fibres were also observed to project from the septal area into other parts of the telencephalon.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00213213
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  • 7
    Digitale Medien
    Digitale Medien
    The distribution of nuclear progesterone receptor in the hypothalamus and forebrain of the domestic hen (1987)
    Springer
    Cell & tissue research 248 (1987), S. 201-205 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Progesterone receptor ; Hypothalamus ; Forebrain ; Immunohistochemistry ; Chicken
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Cell nuclei containing progesterone receptor were identified immunohistochemically in the hypothalamus and forebrain of the domestic hen using an antiserum to the steroid binding “B” subunit (110 kDa) of chicken oviduct progesterone receptor and the avidin-biotin complex procedure. Cell nuclei containing progesterone receptor were widely distributed in the anterior, medial and basal hypothalamus with the highest density occurring in the lamina terminalis and the preoptic area. Abundant, though less intensely reacting progesterone receptor was present in cell nuclei in the tuberal infundibular area and in the internal zone of the median eminence. A large group of cell nuclei containing progesterone receptor occurred in the dorsal anterior hypothalamus between the anterior commissure and the lateral ventricle. This group of nuclei extended anteriorly into the telencephalon. A small number of cell nuclei containing progesterone receptor was also found in the ventral telencephalon in the region of the nucleus accumbens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01239981
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