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  • 1
    Electronic Resource
    Electronic Resource
    Interferon-neutralizing antibodies in a patient treated with human fibroblast interferon (1981)
    [s.l.] : Nature Publishing Group
    Nature 289 (1981), S. 496-497 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The IFN used in therapy was a diploid cell-strain IFN (HuIFN-0) (Rentschler Co., Laupheim) produced in human foreskin fibroblasts (FS-4) by superinduction with poly(I o C), cycloheximide and actinomycin D. The IFN preparations were purified and concentrated using perchloric acid and ammonium ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/289496a0
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  • 2
    Electronic Resource
    Electronic Resource
    Partial Purification of a Polypeptide Extract Derived From Ovine Pineal That Suppresses the Growth of Human Melanoma Cells In Vitro (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of pineal research 6 (1989), S. 0 
    Details
    ISSN: 1600-079X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A combination of gel filtration and reverse-phase high performance liquid chromatography with postcolumn antitumour assay has been developed. A melatonin insensitive human melanoma cell strain was used to guide the purification of the antitumour effect of an ovine pineal aqueous extract (MW 1,000 to 10,000) that possessed the ability to decrease the hypophysiotropic activity of rat and mice hypothalami in vitro. This allows a specific identification of a pined factor (MW 2,000 to 6,000) that inhibits the growth of human melanoma cells at a dose of 0.47 mg/ml medium. It was shown that the activity of this pineal compound differs from structures known to be present in the pineal, such as melatonin, pteridines, and β-carbolines. There appears to be evidence for a peptidic nature of this pineal antitumour factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-079X.1989.tb00435.x
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  • 3
    Electronic Resource
    Electronic Resource
    Growth-inhibiting effect of crude pineal extracts on human melanoma cellsin vitro is different from that of known synthetic pineal substances (1987)
    Springer
    Journal of neural transmission 69 (1987), S. 299-311 
    Details
    ISSN: 1435-1463
    Keywords: Pineal extracts ; human melanoma cells ; growth-inhibiting effect ; synthetic pineal compounds and analogues ; in vitro experiments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effect was studied of a number of synthetic indoleamines, pteridines,β-carbolines, of AVT and of crude extracts from rat and ovine pineal glands on human melanoma cellsin vitro. The identified pineal substances as well as some of their analogues showed an inhibitory effect only at non-physiologically high concentrations. However, crude pineal extracts were more active than the synthetic pineal substances tested. They contain a compound which may have a tumor-inhibiting potency comparable to that of methotrexate but a different mechanism of action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01244350
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  • 4
    Electronic Resource
    Electronic Resource
    Partial purification of (a) low molecular weight ovine pineal compound(s) with an inhibiting effect on the growth of human melanoma cells in vitro (1988)
    Springer
    Journal of neural transmission 73 (1988), S. 135-155 
    Details
    ISSN: 1435-1463
    Keywords: Growth inhibition ; purification ; melanoma cells ; anti-tumor factor ; ovine pineal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An in vitro human melanoma cell assay was used to work up the partial purification of (a) low molecular weight (MW) substance(s) from aqueous extracts of ovine pineal tissue shown to contain a growth-inhibiting activity. A combination of paper chromatography, ion-exchange and reversephase high performance liquid chromatography with post-column antitumor assay has been developed. This allows a specific identification of an ovine pineal factor (MW〈500) which inhibits the growth of human melanoma cells in vitro. The substance was partially purified to about 1,000 times as compared to the IC100-value of the starting material (retentate5). The growth inhibition of human melanoma cells in culture was complete at a dose of 0.1 μg/ml of purified pineal factor(s). It was demonstrated that the activity of this pineal compound differs from some substances known to be present in the pineal, such as melatonin, serotonin, peridines and β-carbolines. The activity was not destroyed by treatment with proteolytic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243385
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  • 5
    Electronic Resource
    Electronic Resource
    Myelopoiesis in vitro is suppressed by hepatitis A virus (1992)
    Springer
    Annals of hematology 64 (1992), S. A132 
    Details
    ISSN: 1432-0584
    Keywords: Hepatitis A virus ; In vitro myelopoiesis ; Long-term bone marrow cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Perturbations of hematopoietic regulation ranging from transient granulocytopenia to rare cases of bone marrow failure are associated with infections due to hepatitis A virus (HAV). In an attempt to elucidate the pathogenetic mechanisms we had previously established that HAV has a direct suppressive effect on human bone marrow progenitors (CFU-GM, -GEMM, BFU-E). These studies were extended to long-term bone marrow cultures (LTBMC): Inoculation of bone marrow mononuclear cells with HAV did not interfere with the establishment of an adherent stromal layer, nor did the inoculation of already established layers cause any morphologically recognizable changes to the stroma. In contrast, a significant and progressive decline of the CFU-GM content in the culture supernatants was demonstrated. HAV antigen was detected by APAAP stain in a subpopulation of stromal cells, and sequential estimations of virus titers in the supernatants provided evidence for viral replication in primary bone marrow cultures. Interferon-gamma and tumor necrosis factor-alpha levels of infected cultures did not differ from those of uninfected controls. These findings argue for a direct suppression of (pre-) CFU-GM by HAV in a model system (LTBMC) lacking an immune defense which would limit viral replication.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01715366
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  • 6
    Electronic Resource
    Electronic Resource
    Hepatitis A-virus in cell culture (1984)
    Springer
    Medical microbiology and immunology 172 (1984), S. 207-213 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Hepatitis A-virus produced in Frhk-4/R cells is used and compared with stool-derived HAV as an antigen in diagnostic test systems. It is shown that the two antigens react identically in the anti-HAV test with anti-HAV-IgG positive human sera. In the anti-HAV-IgM test, too, stool-derived HAV and cell-culture-produced HAV react very similarly. The titres of anti-HAV-IgG and anti-HAV-IgM positive sera, obtained with stool and cell-culture HAV in quantitative antibody determinations, are shown to be identical. It is shown that the problem of HAV antigen production can now be solved by propagation of HAV with Frhk-4/R cells and that this antigen is very useful in diagnostic test systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02123715
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  • 7
    Electronic Resource
    Electronic Resource
    Hepatitis A-virus particles in stools of patients from a natural hepatitis outbreak in Germany (1977)
    Springer
    Medical microbiology and immunology 163 (1977), S. 209-214 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract During a hepatitis outbreak in Southern Germany 27 nm particles were visualized by immune electron microscopy in stools of two patients. These particles were sereologically identical or similar to hepatitis A-virus particles identified in the USA. The buoyant density of these particles was 1.34 g/cm3 as shown by cesium chloride density centrifugation. The particles were first observed in small numbers in a stool obtained 11 days, and in large numbers in stools obtained 6 and 7 days before the onset of jaundice. Few particles were seen on the day of the onset of jaundice and none thereafter. In both patients a sereoconversion to hepatitis A-virus as judged by immune electron microscopy could be demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02126679
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  • 8
    Electronic Resource
    Electronic Resource
    Hepatitis A-virus in cell culture (1984)
    Springer
    Medical microbiology and immunology 173 (1984), S. 9-17 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A test system for the detection of neutralizing antibodies against hepatitis A-virus (anti-HAV-Nt) is presented. The anti-HAV-Nt assay is performed with Frhk-4/R cells and the hepatitis A virus (HAV) strain GBM/Frhk-4/R which has been adapted to these cells. Non-neutralized HAV is demonstrated 14 days after infection of Frhk-4/R cells using a radio-immunoassay for detecting newly grown HAV. The influence of differing amounts of HAV on the anti-HAV-Nt titre and the effect of variations in incubation time of virus-serum mixtures are described. The time course of anti-HAV-Nt is shown in sera from a hepatitis-A patient which were taken at different stages of the disease. Anti-HAV-Nt is compared with anti-HAV and anti-HAV-IgM. It is shown that anti-HAV-Nt correlates closely with anti-HAV and separated anti-HAV-IgG, but only slightly with anti-HAV-IgM. The test system presented makes possible the demonstration of neutralizing antibodies against HAV in gamma-globulin preparations and during vaccination studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02123564
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  • 9
    Electronic Resource
    Electronic Resource
    Application of a solid-phase radioimmunoassay and immune electron microscopy for hepatitis A in diagnosis and research (1978)
    Springer
    Medical microbiology and immunology 166 (1978), S. 187-194 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract With crude virus suspensions from stool and antibodies from hepatitis-A patients, a solid-phase radioimmunoassay (RIA) for detection of hepatitis virus A (HVA) and antibodies against hepatitis virus A (anti-HVA) has been developed. Examples for the application of this test are demonstrated. Virus particles from the stools of the two patients were further characterized. Serologically, they were identical or very similar to the MS-1 strain. Isopycnic CsCl-gradient centrifugation of both strains revealed two peaks, but the particles of different densities did not differ in size or serologically. A modification of the RIA was also useful for determination of IgM antibodies in patients' sera fractionated by sucrose-density centrifugation. The application of the RIA method for serologic epidemiology is demonstrated by a comparison of anti-HVA prevalence in German and non-German women residing in Germany.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02121149
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  • 10
    Electronic Resource
    Electronic Resource
    Hepatitis A virus in cell culture (1981)
    Springer
    Medical microbiology and immunology 170 (1981), S. 83-89 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The propagation and adaptation of hepatitis A virus (HAV) in human embryo kidney cells (HKC) is shown. The growth curve of HAV in the first passage through HKC is compared to the growth curve in the tenth passage through HKC. It is shown that in the course of 18 passages through HKC, HAV adapted to these cells causing the virus to grow much more rapidly. The cell-bound HAV is compared to the HAV released in the cell-culture supernatant during the ninth passage through HKC. The HAV from the tenth passage through HKC is shown to be able to replicate also in a human embryo fibroblast strain (HFS). Furthermore, adaptation of the HAV to HFS is demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02122672
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