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  • 1
    Electronic Resource
    Electronic Resource
    Identification of young megakaryocytes by immunofluorescence and cytophotometry (1978)
    Springer
    Annals of hematology 37 (1978), S. 265-270 
    Details
    ISSN: 1432-0584
    Keywords: Knochenmark ; Megakaryozytäre Vorläuferzellen ; Megakaryopoese ; Immunfluoreszenz ; Antithrombozytenserum ; Zytophotometrie ; Bone marrow ; Megakaryocytic precursor cells ; Megakaryopoiesis ; Immunofluorescence ; Antithrombocytic serum ; Cytophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The DNA-content of fluoresceine-labeled platelet antigen containing cells of mouse bone marrow was measured. For immunofluorescence highly specific anti-mouse-platelet-serum and fluoresceine-conjugated antigammaglobuline was used, applying the “sandwich” technique. Three hundred panoptically identifiable megakaryocytes served as control group. The DNA-polyploidization pattern of megakaryocytes and immunofluorescence positive cells was almost identical. However, among the immunofluorescence positive cells a considerable amount of cells showed DNA-values lower than 4c, whereas the megakaryocytes of the Pappenheim stained smears revealed no DNA values lower than 4c. The percentages of diploid and tetraploid cells, respectively, was 6 and 7% compared with 0 and 1% of panoptically identifiable megakaryocytes. The results suggest that young megakaryocytic cells with diploid and tetraploid DNA-values can be detected by immunofluorescence technique, indicating that the flow from the uncommited to the committed megakaryocytic precursor cell appears at this early stage of megakaryocyte production.
    Notes: Zusammenfassung Es wurde der DNA-Gehalt von fluoreszeinmarkierten, Thrombozytenantigen enthaltenden Zellen im Knochenmark der Maus untersucht. Die Immunfluoreszenz wurde mit hochspezifischem Anti-Mäuse-Thrombozyten-Serum nach der „sandwich“-Methode mit fluoreszeinkonjugiertem Antigammaglobulin durchgeführt. Als Vergleich dienten 300 nach panoptischen Kriterien differenzierbare Megakaryozyten. Das DNA-Verteilungsmuster der Megakaryozyten und der immunfluoreszenzpositiven Zellen war weitgehend identisch. Allerdings wiesen die immunofluoreszenzpositiven Zellen DNA-Werte auch unterhalb 4c auf, während die im Pappenheim-Präparat differenzierbaren Megakaryozyten keine DNA-Werte in diesem Bereich erkennen ließen. Der Anteil diploder bzw. tetraploider immunfluoreszenzpositiver Zellen betrug 6 bzw. 7% im Vergleich zu 0 bzw. 1% bei den panoptisch differenzierbaren Megakaryozyten. Die Ergebnisse lassen den Schluß zu, daß mit Hilfe der Immunfluoreszenz megakaryozytäre Zellen mit diploidem bzw. tetraploidem DNA-Gehalt nachgewiesen werden können. Dies deutet darauf hin, daß der Einstrom der Zellen aus dem undeterminierten Vorläuferzellkompartment in das determinierte Kompartment bereits auf dieser frühen Stufe der Megakaryozytenproduktion stattfindet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01539662
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  • 2
    Electronic Resource
    Electronic Resource
    Wissenschaftlicher Teil (1983)
    Springer
    Archives of gynecology and obstetrics 235 (1983), S. 234-361 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02428738
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  • 3
    Electronic Resource
    Electronic Resource
    Adjuvante Chemotherapie mit LF (Leukeran/Fluorouracil) beim primären Mammakarzinom mit positiven axillären Lymphknoten (kooperative Studie Heidelberg) (1981)
    Springer
    Archives of gynecology and obstetrics 232 (1981), S. 281-282 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02429567
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  • 4
    Electronic Resource
    Electronic Resource
    Combination chemotherapy (VAC/FMC) with immunostimulation in metastatic breast cancer: A randomized study comparing different times and routes of administration of corynebacterium parvum (1984)
    Springer
    Journal of molecular medicine 62 (1984), S. 162-167 
    Details
    ISSN: 1432-1440
    Keywords: Metastatic breast cancer ; Corynebacterium parvum ; Comparison of different times and routes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary From January 1978 to December 1980, 222 patients with metastatic breast cancer were included into a prospective multicenter trial. All patients were treated once a month with six cycles of VAC- (vincristine, adriamycin, cyclophosphamide) chemotherapy, followed by FMC (5-fluor-ouracil, methotrexate, cyclophosphamide) until progression was documented. By random assignment, the patients received immunostimulation with Corynebacterium parvum (CP) by one of four methods: subcutaneous (SC) on either day 1 or day 14, intravenous (IV) on either day 1 or day 14. The 214 evaluable patients were equally distributed to the four arms. The rates of complete or partial response to VAC/FMC plus CP did not differ significantly between the treatment groups. Of our patients, 22–33% were definite treatment failures. The Kaplan-Meier curves of duration of remission (medians 14 vs. 9 vs. 13 vs. 11 months) did not differ significantly. Only small differences in survival were noted among the four study groups (medians 15.4 vs. 17.5 vs. 17.2 vs. 13.0 months). However, complete and partial responders lived significantly longer (Log rank testP=0.008), when CP was given on day 14 by the SC rather than IV route (29+ vs. 14.3 months). Patients in the four study groups were treated with virtually identical doses of VAC/FMC chemotherapy. Patients receiving CP intravenously on day 14 experienced significantly lower mean leukocyte counts than patients in the other groups. Many patients suffered from high temperature (requiring treatment with antipyretics) and severe gastrointestinal toxicity, particularly when CP was given IV on day 1 together with the chemotherapy. Sixteen patients developed skin ulcers following repeated SC injections of CP. They showed a 4-month longer median survival than patients without these local reactions. Taken together, the results suggest that adding CP in the ways tested to monthly VAC/FMC chemotherapy is of no benefit to patients with metastatic breast cancer.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01731638
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  • 5
    Electronic Resource
    Electronic Resource
    Aktueller Stand der adjuvanten Chemo- und Immuntherapie des Kolonkarzinoms (1999)
    Springer
    Der Onkologe 5 (1999), S. 30-34 
    Details
    ISSN: 1433-0415
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Die Etablierung von adjuvanten Therapieverfahren bei kolorektalen Karzinomen seit Anfang der 90er Jahre ist das Ergebnis einer intensiven klinischen Forschung. Die uns heute vorliegenden Ergebnisse zahlreicher prospektiv randomisierter, multizentrischer Therapiestudien aus westlichen Ländern belegen eindrucksvoll, daß mit Hilfe postoperativer adjuvanter Therapien bei Mittelstadien kolorektaler Karzinome eine beträchtliche Verbesserung der Gesamtprognose erzielt werden kann.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007610050320
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  • 6
    Electronic Resource
    Electronic Resource
    Proliferation characteristics of erythroblasts in chronic renal failure (1977)
    Springer
    Annals of hematology 35 (1977), S. 45-50 
    Details
    ISSN: 1432-0584
    Keywords: Erythroblast proliferation ; Chronic renal failure ; Cytophotometry and autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das Proliferationsverhalten der Erythroblasten im Knochenmark von Patienten mit chronischem Nierenversagen wurde mit Hilfe der kombinierten zytophotometrischen DNA-Messung und autoradiographischen in vitro3H-TdR-Markierung untersucht. In den meisten Fällen wurde eine deutliche Verminderung der3H-TdR-Markierung gefunden. Dabei war der Anteil unmarkierter diploider (G0 + G1) und tetraploider Zellen erhöht. Die Ergebnisse lassen sowohl eine gestörte Induktion der DNA-Synthese der Ruhezellen als auch eine Blockierung der Zellen in G2 vermuten, die für die ineffektive Zellproduktion bei dieser Erkrankung ursächlich sind.
    Notes: Summary Proliferation of bone marrow erythroblasts in patients with chronic renal disease was studied by combined application of cytophotometric measurement of the DNA content and3H-TdR labeling in vitro. A striking decrease of3H-TdR labeling was found in most of the cases. The proportion of diploid (G0 + G1) as well as of tetraploid unlabeled cells (G2) was increased. The data suggest a disturbed induction of DNA synthesis of resting cells as well as an arrest of cells in G2 being responsable for ineffective cell production in this disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01006963
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  • 7
    Electronic Resource
    Electronic Resource
    Diploid and tetraploid precursors of megakaryocytes in normal human bone marrow detected by immunofluorescence (1987)
    Springer
    Annals of hematology 55 (1987), S. 459-466 
    Details
    ISSN: 1432-0584
    Keywords: Bone marrow ; Megakarocyte precursor cells ; Immunofluorescence ; Antiplatelet antibody ; Cytophotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A sequential preparation method is described which allows immunological identification, morphological characterization, cytophotometric determination of relative DNA content of the megakaryocyte lineage as well as quantitation of megakaryocyte precursors in human bone marrow aspirates. We compared several monoclonal (anti-GP IIIa and HD 19) and polyclonal (A 225, RAHPS) antiplatelet antibodies for immunoflurescent staining. Among the identified cells, a small number of cells showing a diploid and tetraploid DNA content were found which must be regarded as promegakaryoblasts, representing 2.5–4.7% of all megakaryocytes. The heterogenous morphology of these precursors in panoptically stained smears is described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00367464
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  • 8
    Electronic Resource
    Electronic Resource
    High-dose cytosine arabinoside and daunorubicin postremission therapy in adults with de novo acute myeloid leukemia Long-term follow-up of a prospective multicenter trial (1995)
    Springer
    Annals of hematology 71 (1995), S. 219-225 
    Details
    ISSN: 1432-0584
    Keywords: Key words De novo AML ; Adults ; HD-Ara-C/DNR consolidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  A total of 149 consecutive de novo AML patients aged 50 years or less (median age = 37 years) were enrolled in this prospective multicenter trial initiated in May 1985. All patients received the same induction and early consolidation therapy with daunorubicin (DNR), cytosine arabinoside (Ara-C), and etoposide (DAV). High-dose Ara-C/DNR therapy included Ara-C at 3 g/m2, in 12 doses (HD-Ara-C/DNR I) and eight doses (HD-Ara-C/DNR II), followed by DNR 30 mg/m2 for 3 days. A complete remission (CR) was achieved in 104 (70%) patients; 61 complete responders received at least one cycle with HD-Ara-C/DNR. If those patients who were transplanted in first CR (n = 26), were not considered, the median relapse-free-survival (MRFS) of the remaining 78 patients was 15 months, with a probability of relapse-free survival (RFS) at 116 months of 30% (95% CI, 20–40%) after a median follow-up of 95 months. The MRFS of the HD-Ara-C/DNR consolidated patients was 25 months, with a probability of RFS at 116 months of 37% (95% CI, 24–50%). If all patients who were transplanted (n = 44) were not considered, the median survival time (MST) was 18 months with a probability of being alive at 118 months of 24% (95% CI, 16–33%). MST of the HD-Ara-C/DNR consolidated patients was 58 months with a survival probability of 46% (95% CI, 31–60%) at 118 months. Prognostic factor analysis did not reveal any significant influence of age, sex, FAB subtype, white blood cell count, hemoglobin level, thrombocyte count, LDH, or response to the first induction course on RFS of the HD-Ara-C/DNR consolidated patients. In summary, HD-Ara-C/DNR consolidation can improve the long-term outcome of a subgroup of de novo AML patients. Further improvement of the outcome seems to depend on the identification of patients with an inferior outcome under that strategy who might benefit from alternative treatment strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01744371
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  • 9
    Electronic Resource
    Electronic Resource
    High-dose cytosine arabinoside and daunorubicin postremission therapy in adults with de novo acute myeloid leukemia (1995)
    Springer
    Annals of hematology 71 (1995), S. 219-225 
    Details
    ISSN: 1432-0584
    Keywords: De novo AML ; Adults ; HD-Ara-C/DNR consolidation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A total of 149 consecutive de novo AML patients aged 50 years or less (median age = 37 years) were enrolled in this prospective multicenter trial initiated in May 1985. All patients received the same induction and early consolidation therapy with daunorubicin (DNR), cytosine arabinoside (Ara-C), and etoposide (DAV). High-dose Ara-C/DNR therapy included Ara-C at 3 g/m2, in 12 doses (HD-Ara-C/DNR I) and eight doses (HD-Ara-C/DNR II), followed by DNR 30 mg/m2 for 3 days. A complete remission (CR) was achieved in 104 (70%) patients; 61 complete responders received at least one cycle with HD-Ara-C/DNR. If those patients who were transplanted in first CR (n=26), were not considered, the median relapsefree-survival (MRFS) of the remaining 78 patients was 15 months, with a probability of relapse-free survival (RFS) at 116 months of 30% (95% CI, 20–40%) after a median follow-up of 95 months. The MRFS of the HD-Ara-C/DNR consolidated patients was 25 months, with a probability of RFS at 116 months of 37% (95% CI, 24–50%). If all patients who were transplanted (n=44) were not considered, the median survival time (MST) was 18 months with a probability of being alive at 118 months of 24% (95% CI, 16–33%). MST of the HD-Ara-C/DNR consolidated patients was 58 months with a survival probability of 46% (95% CI, 31–60%) at 118 months. Prognostic factor analysis did not reveal any significant influence of age, sex, FAB subtype, white blood cell count, hemoglobin level, thrombocyte count, LDH, or response to the first induction course on RFS of the HD-Ara-C/DNR consolidated patients. In summary, HD-Ara-C/DNR consolidation can improve the long-term outcome of a subgroup of de novo AML patients. Further improvement of the outcome seems to depend on the identification of patients with an inferior outcome under that strategy who might benefit from alternative treatment strategies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01744371
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  • 10
    Electronic Resource
    Electronic Resource
    A multicentre study on intensive induction and consolidation therapy in acute myelogenous leukaemia (1988)
    Springer
    Annals of hematology 56 (1988), S. 233-236 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320111
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