ISSN:
1432-1440
Keywords:
Pulmonary sarcoidosis
;
Immunology
;
Lung T-lymphocytes
;
Immunoenzyme technology
;
Bronchoalveolar lavage
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The aim of this study was to determine the value of the destination of T-lymphocyte subpopulations in bronchoalveolar lavage (BAL) fluid for the assessment of disease activity in pulmonary sarcoidosis in comparison with other tests of activity, i.e., lung function tests, angiotensin-converting enzyme (ACE), and gallium lung scan. According to clinical and roentgenographic data, 20 patients with biopsy-proven sarcoidosis were classified as having either active (nine patients) or inactive (11 patients) disease. Helper and suppressor T-lymphocyte subsets in BAL were identified by the peroxidase-antiperoxidase method performed on glass slides using murine monoclonal antibodies (OKT 3 for pan T-, OKT 4 for helper, and OKT 8 for suppressor T-lymphocytes). Patients with active sarcoidosis had significantly higher proportions of lung helper cells (90±5% of T-lymphocytes) than did patients with inactive sarcoidosis (65±11%) or control patients (63±7%),P〈0.001. The proportion of lung helper cells correlated significantly with vital capacity (r=−0.72,P〈0.001), with total lung capacity (r=−0.60,P〈0.01), with PaO2 at rest (r=−0.46,P〈0.05) and during exercise (r=−0.54,P〈0.05), and with CO-diffusing capacity/1 lung volume (r=−0.53,P〈0.05). There was, however, no correlation between lung lymphocyte subsets and serum ACE or gallium lung scan, nor between ACE and gallium scan. Although ACE and gallium uptake were higher in active sarcoidosis than in inactive disease, considerable overlap existed between groups, and the difference was not significant. Thus, the proportion of helper cells in BAL fluid appears to be a most useful parameter to assess activity of pulmonary sarcoidosis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01485026
Permalink
