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  • 1
    Electronic Resource
    Electronic Resource
    Studies on the Primary Structure of the Messenger RNA from Phage T4. Frequencies of the Mono-, Di-, and Trinucleotides Obtained from a Digest with Pancreatic Ribonuclease* (1964)
    s.l. : American Chemical Society
    Biochemistry 3 (1964), S. 1010-1014 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00896a001
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  • 2
    Electronic Resource
    Electronic Resource
    Involvement of a Phage T4 σ Factor and an Anti-terminator Protein in the Transcription of Early T4 Genes in vivo (1970)
    [s.l.] : Nature Publishing Group
    Nature 225 (1970), S. 1012-1016 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The existence of an rIIB promoter site not recognized by the Escherichia coli σ factor is demonstrated. Early synthesis of lysozyme messengers seems to result from the lack of termination of pre-early ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/2251012a0
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  • 3
    Electronic Resource
    Electronic Resource
    Localization of RNA polymerase B and histones in the nucleus of primary spermatocytes of Drosophila hydei, studied by immunofluorescence microscopy (1981)
    Springer
    Chromosoma 82 (1981), S. 399-407 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract By means of indirect immunofluorescence microscopy, we have studied the distribution of RNA polymerase B, of the nucleosomal histones H2b, H3, and H4 and of histone H1, in nuclei of primary spermatocytes of Drosophila hydei. RNA polymerase B and histones, including H1, are found to be present on the loop structures of the Y chromosome. The nucleolus stains only for the histones, but not for RNA polymerase B. Various mutants deficient for some of the loops or altering their morphology, were used to identify the individual chromosomal segments. In growing spermatocytes of the genetic constitution X/0, autosomes and the chromosome X react strongly with antibodies against RNA polymerase B, but not with antibodies against histones. The results suggest that the autosomes, the chromosome X and the Y chromosomal loop structures, with the exception of the nucleolus, are transcribed mostly by RNA polymerase B.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285765
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  • 4
    Electronic Resource
    Electronic Resource
    Telomeric sequences derived from laser-microdissected polytene chromosomes (1989)
    Springer
    Chromosoma 98 (1989), S. 351-357 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recently it has been shown that microdissection can be improved to very high precision if a laser coupled into a microscope is used. A laser microbeam, generated by an excimer pumped dye laser, allows chromosomes to be cut into slices of less than 0.5 μm. Here it is shown, that single copy DNA probes prepared from Drosophila chromosomes by laser microdissection and microcloning relocalize to the chromosomal regions from which they are derived. The combination of laser technique and microcloning provides an advantageous approach for rapid genetic analysis with potential for the study of genetic diseases and genome mapping.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292388
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  • 5
    Electronic Resource
    Electronic Resource
    Coding strategy of the S and M genomic segments of a hantavirus representing a new subtype of the Puumala serotype (1995)
    Springer
    Archives of virology 140 (1995), S. 2011-2026 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The hantavirus strain Vranica was previously reported to have been isolated from a bank vole in Bosnia-Hercegovina and associated with the occurrence of hemorrhagic fever with renal syndrome (HRFS) in humans. The complete cDNA nucleotide sequences of the small (S) and medium (M) genomic RNA segments of this virus were determined. Major open reading frames were found in the S and M segment between nucleotide positions 43 and 1341 coding for a polypeptide of 433 amino acid residues and between nucleotide positions 41 and 3 484 coding for 1 148 amino acid residues, respectively. The analysis and the alignment of the nucleotide and the derived amino acid sequences with known sequences of other hantavirus strains demonstrate that Vranica resembles Swedish strains and represents a new virus subtype of the Puumala serotype distinct from the subtypes represented by virus strains CG18–20 and Sotkamo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01322689
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  • 6
    Electronic Resource
    Electronic Resource
    Preface (1991)
    Springer
    Molecular biology reports 15 (1991), S. 97-97 
    Details
    ISSN: 1573-4978
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00365538
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  • 7
    Electronic Resource
    Electronic Resource
    The gene upstream of DmRP128 codes for a novel GTP-binding protein of Drosophila melanogaster (1994)
    Springer
    Molecular genetics and genomics 242 (1994), S. 391-398 
    Details
    ISSN: 1617-4623
    Keywords: Drosophila melanogaster ; RNA polymerase III second-largest subunit ; Upstream gene ; GTP-binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Upstream of the gene coding for the second-largest subunit of RNA polymerase III (DmRP128) we have found another gene (128up), which is transcribed in the same direction as the RNA polymerase gene. The intergenic distance between the 3′ end of 128up mRNA and the 5′ end of DmRP128 mRNA is only about 100 bp. Transcripts of 128up are present at a much higher level than DmRP128 RNA in Drosophila Schneider 2 cells, embryos, and adult flies. Two transcription start points, seven nucleotides apart, are found for 128up compared to multiple scattered starts for DmRP128. Sequence analysis of 128up cDNA reveals that the gene codes for a 41 kDa protein with homology to GTP-binding proteins and matching four of the structural sequence motifs characteristic of the superfamily of GTPases. Bacterially expressed 128up protein fused to maltose-binding protein specifically binds GTP. Sequences closely related to the 128up protein are found in species as distant as Halobacterium, yeast or mouse; the murine protein is 80% identical to 128up. This evolutionary conservation is indicative of an important, but as yet unknown, physiological role. In accordance with the sequence conservation, antibodies against 128up specifically cross-react with mouse 3T3 cells and human Hep2 cells where the subcellular localization of the protein is predominantly perinuclear. We propose that 128up is a member of a novel class of GTP-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281788
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  • 8
    Electronic Resource
    Electronic Resource
    Specificity of σ-dependent binding of RNA polymerase to DNA (1972)
    Springer
    Molecular genetics and genomics 118 (1972), S. 199-207 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Although a large number of E. coli RNA polymerase molecules can bind to phage T3 DNA, not more than three remain bound per DNA template after addition of poly inosinic acid (poly I) which has a high affinity for the enzyme. These stable complexes are able to initiate RNA chains without lag as the enzyme is resistant against rifampicin if substrate is added simultaneously with the drug. Poly I resistant complexes decay very rapidly in the cold (Fig. 2) and are not formed in the absence of the polymerase σ factor (Table 2). The data provide additional support for the idea that the σ factor effects the binding of the enzymes to specific sites on the DNA template.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00333456
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of a ts β′ mutant RNA polymerase ofEscherichia coli (1976)
    Springer
    Molecular genetics and genomics 147 (1976), S. 337-341 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary RNA polymerase isolated from ts XH56, a conditional lethal mutant unable to grow and synthesize RNA at 42°, was found to be temperature sensitive in vitro. The mutation affects the β′ subunit as determined by mixed reconstitution of isolated subunits from wild-type and mutant enzyme. The mutant RNA polymerase is unstable; addition of glycerol stabilizes the enzyme and increases its activity on native DNA. In addition, the mutant enzyme is sensitive to high ionic strength. Both high temperature and high ionic strength do not affect chain elongation; thus, the mutation renders the enzyme unable either to bind to or melt out promotor sites. From these data we conclude that the β′ subunit plays an important role in promotor selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582886
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  • 10
    Electronic Resource
    Electronic Resource
    Seroprevalence of hantavirus antibodies in Germany as determined by a new recombinant enzyme immunoassay (1995)
    Springer
    European journal of clinical microbiology & infectious diseases 14 (1995), S. 305-313 
    Details
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to elucidate the epidemiological importance of hemorrhagic fever with renal syndrome in Germany, the prevalence of antibodies against hantaviruses was determined in 13,358 sera from residents of various geographic regions, 1,284 sera from occupational risk groups and 287 sera from chronic hemodialysis patients. Serological investigations were performed using a highly specific transferable solid phase enzyme immunoassay based on the recombinant nucleocapsid proteins of a Hantaan and a Puumala serotype strain. The overall antibody prevalence was found to be 1.68 %. In the serum panels from western and southern Germany, it was determined to be 1.83 % on average in contrast to only 0.8 % in the panel from eastern Germany. An endemic focus revealing an antibody prevalence of 3.12 % was detected in a low-mountain area called Suebian Alb, which is located in the federal state of Baden-Württemberg. Occupational risk groups and a group of chronic hemodialysis patients showed a significantly elevated antibody prevalence ranging from 3.3 % to 10 %. The Puumala serotype was found to be the prevailing virus, but the percentage of sera predominantly recognizing the Hantaan nucleocapsid protein increased towards the south and the east and was significantly elevated in dialysis patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02116523
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