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  • 1
    Electronic Resource
    Electronic Resource
    m-Hydroxybenzyl alcohol dehydrogenase from Penicillium urticae (1972)
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 1108-1114 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00756a026
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Conversion of 6-methylsalicylic acid into patulin by Penicillium urticae (1972)
    s.l. : American Chemical Society
    Biochemistry 11 (1972), S. 1102-1107 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00756a025
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    MECHANISM OF ACTION OF ALKYLATING AGENTS (1969)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 163 (1969), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1969.tb24877.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ammonium repression of antibiotic and intracellular proteinase production inPenicillium urticae (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 447-455 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this study the addition of ammonium ions (5–30 mM) toPenicillium urticae shake-flask cultures before, during and after the onset of polyketide biosynthesis was examined in a time-dependent manner for its repressive effect on metabolites and a marker enzyme of the patulin pathway and on the intracellular proteinases that also appear during the non-growth or idiophase. A study of the effect of ammonium ion addition, showed that both secondary enzyme and proteinase appearance were maximally delayed if the addition was made before the normal 7 h period of derepression/induction. If added during this period the effect of ammonium ions was progressively less. A reduction in the extracellular ammonium ion concentration from 30 to 4mM appeared to be required to initiate the derepression/induction process. Adding ammonium ions during the appearance of secondary enzymes caused a rapid decrease in specific activity, about 67% for the patulin pathway enzyme and 12% for proteinase. Nitrogen repression exerts a much stronger effect on the expression of polyketide genes as opposed to proteinase genes. Both patulin pathway enzymes and proteinases are subjected to proteolysis, but the proteinases retain much of their activity, whereas the polyketide biosynthetic enzymes do not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982534
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Ammonium repression of antibiotic and intracellular proteinase production in Penicillium urticae (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 447-455 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this study the addition of ammonium ions (5–30 mM) to Penicillium urticae shake-flask cultures before, during and after the onset of polyketide biosynthesis was examined in a time-dependent manner for its repressive effect on metabolites and a marker enzyme of the patulin pathway and on the intracellular proteinases that also appear during the non-growth or idiophase. A study of the effect of ammonium ion addition, showed that both secondary enzyme and proteinase appearance were maximally delayed if the addition was made before the normal 7 h period of derepression/induction. If added during this period the effect of ammonium ions was progressively less. A reduction in the extracellular ammonium ion concentration from 30 to 4 mM appeared to be required to initiate the derepression/induction process. Adding ammonium ions during the appearance of secondary enzymes caused a rapid decrease in specific activity, about 67% for the patulin pathway enzyme and 12% for proteinase. Nitrogen repression exerts a much stronger effect on the expression of polyketide genes as opposed to proteinase genes. Both patulin pathway enzymes and proteinases are subjected to proteolysis, but the proteinases retain much of their activity, whereas the polyketide biosynthetic enzymes do not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982534
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Ammonium repression of antibiotic and intracellular proteinase production inPenicillium urticae (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 447-455 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract In this study the addition of ammonium ions (5–30 mM) toPenicillium urticae shake-flask cultures before, during and after the onset of polyketide biosynthesis was examined in a time-dependent manner for its repressive effect on metabolites and a marker enzyme of the patulin pathway and on the intracellular proteinases that also appear during the non-growth or idiophase. A study of the effect of ammonium ion addition, showed that both secondary enzyme and proteinase appearance were maximally delayed if the addition was made before the normal 7 h period of derepression/induction. If added during this period the effect of ammonium ions was progressively less. A reduction in the extracellular ammonium ion concentration from 30 to 4mM appeared to be required to initiate the derepression/induction process. Adding ammonium ions during the appearance of secondary enzymes caused a rapid decrease in specific activity, about 67% for the patulin pathway enzyme and 12% for proteinase. Nitrogen repression exerts a much stronger effect on the expression of polyketide genes as opposed to proteinase genes. Both patulin pathway enzymes and proteinases are subjected to proteolysis, but the proteinases retain much of their activity, whereas the polyketide biosynthetic enzymes do not.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00939034
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Semi-continuous production of the antibiotic patulin by immobilized cells of Penicillium urticae (1983)
    Springer
    Biotechnology letters 5 (1983), S. 125-130 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Conidia of Penicillium urticae, immobilized in κ-carrageenan beads, were germinated in situ to form a patulin producing cell mass by incubating these beads in a growth supporting medium. When these in situ grown immobilized cells were repeatedly transferred and incubated in a nitrogen-free production medium they exhibited a greater than three fold increase in the half-life of patulin production as compared with similarly treated free cells. When conidia were first germinated and grown to form a free cell mass and then immobilized, both the half-life and total patulin production were much less than that for free cells or in situ grown immobilized cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00132171
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  • 8
    Electronic Resource
    Electronic Resource
    In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture (1983)
    Springer
    Biotechnology letters 5 (1983), S. 573-578 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to conventional batch cultures. Exclusion of FCS from the dialysing growth medium resulted in cell death, indicating that a low molecular weight serum component was necessary for growth. This study indicates that nutrient depletion limits hybridoma cell growth in conventional batch culture and also provides a simple and economical means of producing relatively high titre monoclonal antibody in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130835
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  • 9
    Electronic Resource
    Electronic Resource
    A manganese requirement for patulin biosynthesis by cultures of Penicillium urticae (1984)
    Springer
    Biotechnology letters 6 (1984), S. 231-236 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Antibiotic production by submerged cultures of Penicillium urticae required manganese supplementation of the media. Thus, manganese supplementation (152 μM) allowed accumulation of patulin to high concentrations (2 μmol/mL), whereas manganese deficiency (1.53 μM) resulted in the accumulation to similar levels of the first committed pathway intermediate, methyl-salicylic acid, without significant patulin accumulation. Preliminary studies suggest that a similar manganese effect may occur in other fungal species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00140042
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  • 10
    Electronic Resource
    Electronic Resource
    Continuous production of patulin by immobilized cells ofPenicillium urticae in a stirred tank reactor (1983)
    Springer
    Biotechnology letters 5 (1983), S. 785-790 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Production of the mycotoxin, patulin, by immobilized cells ofPenicillium urticae was chosen as a model system to study the formation of secondary metabolites on a long term basis. Spores of the fungus were immobilized in carrageenan beads and allowed to germinate in situ by incubation in a growth medium. Production of the antibiotic by the populated biocatalyst was then followed in a continuous stirred tank reactor (CSTR) under three different regimes of nutrient feed. Using a nitrogen free feed, antibiotic production gradually declined but the cells could be re-activated by addition of a diluted growth medium. Prolonged production of patulin (up to 440 h) was observed when a source of nitrogen (ie. yeast extract) was supplied to the feed medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01386648
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