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  • 1
    Electronic Resource
    Electronic Resource
    In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture (1983)
    Springer
    Biotechnology letters 5 (1983), S. 573-578 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to conventional batch cultures. Exclusion of FCS from the dialysing growth medium resulted in cell death, indicating that a low molecular weight serum component was necessary for growth. This study indicates that nutrient depletion limits hybridoma cell growth in conventional batch culture and also provides a simple and economical means of producing relatively high titre monoclonal antibody in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00130835
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Continuous production of patulin by immobilized cells ofPenicillium urticae in a stirred tank reactor (1983)
    Springer
    Biotechnology letters 5 (1983), S. 785-790 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Production of the mycotoxin, patulin, by immobilized cells ofPenicillium urticae was chosen as a model system to study the formation of secondary metabolites on a long term basis. Spores of the fungus were immobilized in carrageenan beads and allowed to germinate in situ by incubation in a growth medium. Production of the antibiotic by the populated biocatalyst was then followed in a continuous stirred tank reactor (CSTR) under three different regimes of nutrient feed. Using a nitrogen free feed, antibiotic production gradually declined but the cells could be re-activated by addition of a diluted growth medium. Prolonged production of patulin (up to 440 h) was observed when a source of nitrogen (ie. yeast extract) was supplied to the feed medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01386648
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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