ISSN:
1420-908X
Keywords:
Soluble guanylyl cyclase
;
Phenothiazines
;
ADP-Ribosylation
;
P19 cells
;
Methylene blue
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Chlorpromazine is a phenothiazine with a structure similar to that of methylene blue. Since methylene blue is a well known inhibitor of nitric oxideinduced cyclic GMP accumulation, we investigated whether chlorpromazine had the same effect. Cyclic GMP accumulation, induced in a mouse teratocarcinoma cell line (P19) by sodium nitroprusside (a nitric oxide releasing agent), was inhibited by both methylene blue (IC50 0.34µM) and chlorpromazine (IC50 35µM). Chlorpromazine's action was probably directed specifically at soluble guanylate cyclase, since the drug had no effect on ADP-ribosylation in rat hippocampus, another nitric oxide-affected, but cGMP-independent event.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02032570
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