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  • 1
    Electronic Resource
    Electronic Resource
    A comparative in vitro study of antibody binding to different stages of the hookwormAncylostoma caninum (1978)
    Springer
    Parasitology research 56 (1978), S. 147-157 
    Details
    ISSN: 1432-1955
    Keywords: Ancylostoma caninum ; Indirect Fluorescent Antibody Technique (IFAT) ; Indirect Immuno Peroxidase Technique (IIPT) ; hookworms ; cryostat sections ; body surface ; cuticle ; dogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Indirect Fluorescent Antibody Technique (IFAT) and the Indirect Immuno Peroxidase Technique (IIPT) have been applied to cryostat sections and intact stages of the hookworm speciesAncylostoma caninum with sera from infected dogs. Especially the role of the body surface (=cuticle (cortex, matrix, basal layer) and hypodermis) in immunity was studied. Using cryostat sections and dead intact stages as the antigen, specific antibody binding was demonstrated round the ovum membrane and the cuticle of all stages of this hookworm species. Cryostat sections of adult worms showed, that it probably is not the cuticle itself that is antigenic, but that the specific reaction that is observed consisted of a layer, covering the cortex of the cuticle. Infective and parasitic living stages, however, showed no antibody binding in contrast to the free-living stages in which specific antibody binding was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930745
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    IgG antibody binding to the outer surface of infective larvae ofAncylostoma caninum (1978)
    Springer
    Parasitology research 58 (1978), S. 91-96 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Applying the indirect fluorescent antibody technique to the infective stages of the hookwormAncylostoma caninum, it appeared that they do not show IgG antibody binding when serum from dogs infected withA. caninum was used in the test (antiserum). However, inhibiting these stages metabolically with azide or with low temperatures, IgG antibody binding to the outer surface was observed. When the inhibitory factors were removed, shedding of fluorescent substances was seen, which were obviously coming from the outer surface of the larvae. This suggests that shedding of the antigen might occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930795
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    The effect of ultrafiltrated and dialysed dog serum on the chemotaxis of infective hookworm larvae ofAncylostoma caninum (1982)
    Springer
    Parasitology research 68 (1982), S. 305-311 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Infective hookworm larvae ofAncylostoma caninum showed chemotaxis when applied on agar plates in a dog serum gradient. The factor(s) that caused this chemotaxis was thermostable and dialysable against PBS. Using ultrafiltration to separate dog serum in several fractions each with a different molecular weight, it was shown that the factor(s) causing chemotaxis had a molecular weight less than 500 Dalton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927408
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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