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  • 1
    Electronic Resource
    Electronic Resource
    Concurrent parasitic infections among patients with onchocerciasis and controls in Sierra Leone, West Africa (1987)
    Springer
    Documenta ophthalmologica 67 (1987), S. 25-32 
    Details
    ISSN: 1573-2622
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Research was performed into the prevalence of concurrent parasite infection among 23 patients with onchocerciasis and 13 onchocerciasis-negative controls in Sierra Leone, West Africa. Stools, urine sediments and bloodsmears were examined for ova, parasites and microfilariae. Results showed the presence of Hookworm, Schistosoma mansoni, Trichuris trichiura and Ascaris lumbricoides. A positive relationship was found between O. volvulus and Hookworm infection. The effects of polyparasitism on immunodiagnosis and the need for further research is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00142694
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Skin penetration of infective hookworm larvae (1977)
    Springer
    Parasitology research 53 (1977), S. 255-262 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Migratory behaviour of Ancylostoma braziliense was studied in relation to the structure of the skin in dogs after primary infections. Data were obtained studying serial sections of lateral skin areas 6 mm in diameter, which had been exposed to larvae. The sections were stained either with Harris' haematoxylin and eosin or with P.A.S. or as outlined by Crossmon. Most of the larvae managed to penetrate the skin within 1/2 hr after the application. Hairs did not seem to constitute sites of entry. The larvae moved into the horny layer where edges of keratinized cells provide uneven spots. They migrated approximately parallel to the surface from the horny layer into the living epidermis and continued into an external root sheath of a hair follicle. They could only leave this site via sebaceous glands for the dermis or via apocrine sweat glands for the hypodermis. Tunnels from the epidermis into the dermis, however, suggested that a direct trans-epidermal migration had occurred. The vessels invaded by larvae were hypodermal lymphatic vessels. The first ones were found in these structures 1/2 h after the onset of the exposure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389941
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Skin penetration of infective hookworm larvae (1977)
    Springer
    Parasitology research 53 (1977), S. 263-266 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The hairless metacarpal foot pads of six hookworm-free puppies were exposed to infective larvae of Ancylostoma braziliense. Serial sections of the biopts stained with Harris' haematoxylin and eosin showed that the infective larvae are able to penetrate the toughest region of canine skin. Pores of eccrine sweat glands did not seem to constitute sites of entry and no larvae were detected in these glands. Larvae were only observed in the epidermis. The histopathology of the infected skin of the foot pads of the puppies was similar to that in human skin with “creeping eruption” as described by Fülleborn (1927). The biopts appeared to consist of hairy skin as well. In the unexposed adjacent hairy skin of the foot pads, larvae were also observed. They were found in the epidermis, hair follicle systems and dermis, suggesting that the migration from the epidermis into deeper tissue depends on the presence of the hair follicle systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389942
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    IgG antibody binding to the outer surface of infective larvae ofAncylostoma caninum (1978)
    Springer
    Parasitology research 58 (1978), S. 91-96 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Applying the indirect fluorescent antibody technique to the infective stages of the hookwormAncylostoma caninum, it appeared that they do not show IgG antibody binding when serum from dogs infected withA. caninum was used in the test (antiserum). However, inhibiting these stages metabolically with azide or with low temperatures, IgG antibody binding to the outer surface was observed. When the inhibitory factors were removed, shedding of fluorescent substances was seen, which were obviously coming from the outer surface of the larvae. This suggests that shedding of the antigen might occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930795
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Skin penetration of infective hookworm larvae (1977)
    Springer
    Parasitology research 53 (1977), S. 155-158 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The lateral skin areas of nine hookworm-free puppies were exposed to infective larvae of Ancylostoma braziliense, Ancylostoma ceylanicum and Ancylostoma caninum. Serial sections, stained with Harris' haematoxylin and eosin, showed that many similarities exist in the migration patterns of the hookworm species used. However, striking differences were observed between Ancylostoma braziliense on the one hand and Ancylostoma ceylanicum and Ancylostoma caninum on the other hand with regard to the speed with which the penetration occurred. Larvae of Ancylostoma braziliense may travel directly from the epidermis into the dermis — apart from migration via hair follicle systems — a path of migration we did not observe in infections with the other species used. Larvae of Ancylostoma braziliense seem to pass the skin more rapidly than those of Ancylostoma ceylanicum and Ancylostoma caninum. Many larvae of Ancylostoma ceylanicum managed to penetrate the skin 2–6 h after the onset of the exposure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380460
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    A comparative in vitro study of antibody binding to different stages of the hookwormAncylostoma caninum (1978)
    Springer
    Parasitology research 56 (1978), S. 147-157 
    Details
    ISSN: 1432-1955
    Keywords: Ancylostoma caninum ; Indirect Fluorescent Antibody Technique (IFAT) ; Indirect Immuno Peroxidase Technique (IIPT) ; hookworms ; cryostat sections ; body surface ; cuticle ; dogs
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The Indirect Fluorescent Antibody Technique (IFAT) and the Indirect Immuno Peroxidase Technique (IIPT) have been applied to cryostat sections and intact stages of the hookworm speciesAncylostoma caninum with sera from infected dogs. Especially the role of the body surface (=cuticle (cortex, matrix, basal layer) and hypodermis) in immunity was studied. Using cryostat sections and dead intact stages as the antigen, specific antibody binding was demonstrated round the ovum membrane and the cuticle of all stages of this hookworm species. Cryostat sections of adult worms showed, that it probably is not the cuticle itself that is antigenic, but that the specific reaction that is observed consisted of a layer, covering the cortex of the cuticle. Infective and parasitic living stages, however, showed no antibody binding in contrast to the free-living stages in which specific antibody binding was demonstrated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00930745
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of ultrafiltrated and dialysed dog serum on the chemotaxis of infective hookworm larvae ofAncylostoma caninum (1982)
    Springer
    Parasitology research 68 (1982), S. 305-311 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Infective hookworm larvae ofAncylostoma caninum showed chemotaxis when applied on agar plates in a dog serum gradient. The factor(s) that caused this chemotaxis was thermostable and dialysable against PBS. Using ultrafiltration to separate dog serum in several fractions each with a different molecular weight, it was shown that the factor(s) causing chemotaxis had a molecular weight less than 500 Dalton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00927408
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Chemotactic attraction of infective hookworm larvae ofAncylostoma caninum by a dog serum factor (1985)
    Springer
    Parasitology research 71 (1985), S. 539-543 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Infective hookworm larvae ofAncylostoma caninum showed chemotaxis on agar plates in a dog serum gradient. This chemotactic behaviour remain unaltered using an ultrafiltrated serum fraction with a molecular weight less than 500. Gelfiltration of this ultrafiltrated fraction revealed a factor with a molecular weight of 480 causing chemotaxis. The chemotactic activity of the factor was destroyed after a pronase treatment. We conclude that the factor could be a polypeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00928357
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    Paper (German National Licenses)
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