Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    NaK-ATPase in rat pancreatic islets (1977)
    Springer
    Diabetologia 13 (1977), S. 235-238 
    Details
    ISSN: 1432-0428
    Keywords: Monovalent cations ; NaK-ATPase ; pancreatic islets ; insulin secretion ; cation transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Shifts in the distribution of the monovalent cations Na+ and K+ between the extra-and intracellular space seem to be important for the secretory response of the β-cell. An attempt was therefore made to study the enzyme responsible for monovalent cation transport, the (NaK)-activated ATPase. In the presence of NaN3 as inhibitor of the mitochondrial Mg-ATPase, a NaK-ATPase with a specific activity of 72 mU×mg protein−1 could be demonstrated in crude membrane preparations of rat pancreatic islets. The enzyme, which was inactive in the absence of Mg++, needed both Na+ and K+ for activation and was inhibited by ouabain and PCMB. The main part of the NaK-ATPase was localized in the microsomal fraction. Glucose, sulphonylureas, somatostatin and diazoxide were with-out effect on NaK-ATPase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01219705
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    The effect of insulin on pyruvate dehydrogenase interconversion in heart muscle of alloxan-diabetic rats (1978)
    Springer
    Diabetologia 14 (1978), S. 135-139 
    Details
    ISSN: 1432-0428
    Keywords: Insulin ; pyruvate dehydrogenase ; enzyme interconversion ; heart muscle ; alloxan-diabetes ; heart perfusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Evidence is presented for regulation by insulin of pyruvate dehydrogenase (PDH) interconversion in rat heart muscle in vivo and in vitro. In the alloxan diabetic rat the active (dephospho) enzyme amounted only to 12% of total PDH and was restored to 42% by insulin. Antilipolytic treatment of the diabetic animals was ineffective, indicating that the action of insulin was independent of a lowering of plasma non-esterified fatty acid concentration. On perfusion of isolated hearts from diabetic rats in the presence of glucose the proportion of pyruvate dehydrogenase in the active form remained low but was fully restored upon addition of insulin (2 mU/ml) to the medium. No effect of insulin was obtained in the absence of glucose. The correlation between the rate of pyruvate decarboxylation in the perfused heart and of pyruvate dehydrogenase activity, in vitro, suggests that in the diabetic heart the entry of pyruvate into the citric acid cycle is largely controlled by covalent modification of the pyruvate dehydrogenase complex rather than by feedback inhibition. The possible role of insulin therein is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01263452
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Der Einfluß von Cortisol auf den Insulineffekt am Fettgewebe in vitro (1964)
    Springer
    Journal of molecular medicine 42 (1964), S. 502-503 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Glucose uptake, especially Glucose-C1- but also Glucose-C6-Oxydation and formation of lactic acid by adipose tissue is stimulated in vitro by insulin. Cortisol in an concentration of 30µg/ml shows a 50% inhibition of this insulineffect. With physiological amounts of cortisol there is a dose-response curve of inhibition which is greatest for the glucose-C1-oxydation. The importance of these data in respect to the diabetogenic action of glucocorticoids is discussed.
    Notes: Zusammenfassung Insulin stimuliert in vitro die Glucoseaufnahme, Glucose-C-1- bzw. -C-6-Oxydation und Milchsäurebildung des Fettgewebes. Bei Zusatz von hohen Dosen Cortisol (30µg/ml) wird dieser Effekt zu ungefähr 50% gehemmt. Bei Einsatz physiologischer Mengen Cortisol (0,003–0,05µg Cortisol/ml) läßt sich eine Dosis-Wirkungsrelation für die Hemmung des Insulineffektes aufstellen, die für die C-1-Oxydation weitaus am steilsten verläuft. Die Bedeutung dieser Befunde für das Verständnis der diabetogenen Wirkung der Glucocorticoide wird diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01484133
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Angeborene nichtsphärocytäre hämolytische Anämie bei familiären Mangel an DPNH- und TPNH-abhängiger Glutathionreduktase der Erythrocyten (1966)
    Springer
    Journal of molecular medicine 44 (1966), S. 571-573 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A decreased activity of both DPNH- and TPNH-Glutathione-Oxido-Reductase (GSSG-Reductase) was found in the erythrocytes of a 16-year-old boy suffering from congenital non-spherocytic haemolytic anaemia. The optimal pH, the inhibition by GSSG and the Michaelis-Menten constants of both enzymes were found to be normal in comparison with controls. A diminished level of the normal enzyme protein rather than alteration of the protein configuration is discussed as a factor in the development of this type of anaemia.
    Notes: Zusammenfassung In den Erythrocyten eines 16jährigen Jungen mit einer angeborenen nichtsphärocytären hämolytischen Anämie wurde eine Aktivitätsminderung der DPNH- und TPNH-abhängigen Glutathionreduktase festgestellt. Da sich die pH-Optima, die Substrathemmung und die Michaelis-Menten-Konstanten beider Enzyme nicht von den Werten eines Normalkollektivs unterschieden, wird als Ursache der Erkrankung der Mangel eines normal strukturierten Enzymproteins und nicht das Vorliegen eines pathologischen Genproduktes diskutiert.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01726591
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Glucagon-empfindliche lipolytische Aktivität im Fettgewebe (1965)
    Springer
    Journal of molecular medicine 43 (1965), S. 175-176 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01484514
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Beitrag zur Pathogenese des akuten Nierenversagens (1967)
    Springer
    Journal of molecular medicine 45 (1967), S. 466-474 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Determinations of the LDH-isoenzymes in the serum of normals, patients with end-stage kidney disease in chronic nephropathies, and patients with acute renal failure together with the simultaneous determinations of the LDH-isoenzymes in renal artery and vein blood of patients without kidney disease showed the following results: 1. There is no evidence for inflow of renal LDH into the blood-stream as determined by the direct examination of the a-v-differencies in patients without renal disease. The LDH structural pattern in the serum of patients without renal disease seems to be little influenced by the healthy kidney. 2. In acute renal failure there is almost always a marked increase in the serum LDH activity as compared to normal kidney function. Also there is a marked difference in the LDH structural pattern of the serum as compared to normal. The reason for this finding is that the regular isoenzyme pattern is covered underneath the organ specific kidney pattern. This isoenzyme distribution is to find also in patients with acute renal failure, where the total activity of the serum LDH is not or no more increased. 3. The inflow of cytoplasmatic fixed renal cell enzymes into the blood stream happens during the initial period of injury in acute renal failure still before the full blown pattern of disturbed renal function is established. It results from ischemic or toxic injury of the tubular epithelial cells, which only seldom ends up with necrosis. In most cases there is morphologically only a marked swelling of the tubular cells seen in specimens of renal intravital biopsy. With these results, in connection with newer morphological results and the findings ofThurau concerning the intrarenal, Na+-dependant regulation mechanism for the glomerular filtration there is a possible explanation for the pathogenesis of the oliguricanuric stage in acute renal failure in men.
    Notes: Zusammenfassung Bestimmungen der Lactatdehydrogenase-Isoenzyme im Serum bei Nierengesunden, Patienten im Terminal- bzw. Präterminalstadium chronischer Nephropathien und Patienten mit akutem Nierenversagen, sowie simultane Bestimmungen der LDH-Isoenzyme im Nierenarterien- und Nierenvenenblut Nierengesunder hatten folgende Ergebnisse: 1. Bei direkter Untersuchung der renalen av-Differenzen ist beim Nierengesunden eine Einschwemmung renaler LDH in die Blutbahn nicht nachzuweisen. Das LDH-Strukturmuster im Serum Gesunder scheint nur wenig vom LDH-Isoenzymmuster der gesunden Niere geprägt zu sein. 2. Bei akutem Nierenversagen ist die LDH-Aktivität im Serum gegenüber der Norm fast immer deutlich erhöht. Dabei weicht das LDH-Isoenzymmuster erheblich von der im Serum Gesunder gefundenen Norm ab. Ursache hierfür ist eine Überlagerung des regelrechten Strukturmusters durch das organspezifische „Nierenmuster“. Diese Verteilung findet sich auch bei Patienten mit akutem Nierenversagen, bei denen die Gesamtaktivität der LDH im Serum nicht oder nicht mehr erhöht ist. 3. Die Einschwemmung cytoplasmatisch gebundener Zellfermente in die Blutbahn erfolgt in der initialen Schädigungsphase eines akuten Nierenversagens noch vor der vollen Ausprägung der renalen Funktionsstörung. Sie ist Ausdruck einer ischämischen oder toxischen Tubulusepithelschädigung, die nur in den seltensten Fällen den Grad der Nekrose erreicht, in den meisten Fällen jedoch morphologisch nur im intravital gewonnenen Nierenpunktionszylinder als ausgeprägte Zellschwellung nachweisbar ist. Aufgrund dieser Befunde bietet sich im Zusammenhang mit neueren morphologischen Befunden und den Untersuchungsergebnissen vonThurau über einen intrarenalen Na+-abhängigen Regelmechanismus für die glomeruläre Filtration eine Erklärung der Pathogenese der Oligurie-Anurie beim akuten Nierenversagen des Menschen an.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01717433
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Adipose tissue development: The role of precursor cells and adipogenic factors (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 803-811 
    Details
    ISSN: 1432-1440
    Keywords: Adipose tissue ; Differentiation ; Obesity ; Preadipocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Obesity is regarded as a heterogeneous syndrome, which may appear in different forms. Various causes have been found to contribute to its pathogenesis. During recent years investigations of adipose tissue cellularity and its dynamic changes have gained growing interest. An important progress was the discovery of adipose tissue precursor cells. These cells have not yet been precisely identified by morphological and biochemical methods in intact tissue. However, due to methodological developments such precursor cells can be cultured both as primary cultures and as established cell lines. These culture systems have proven to be valuable models for the study of the processes involved in the formation of new fat cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01727474
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Adipose tissue development: The role of precursor cells and adipogenic factors (1987)
    Springer
    Journal of molecular medicine 65 (1987), S. 812-817 
    Details
    ISSN: 1432-1440
    Keywords: Adipose tissue ; Differentiation ; Obesity ; Preadipocytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Cell culture systems have proven to be valuable models for the study of the processes involved in the formation of new fat cells. Two separate steps may be distinguished in adipocyte development. First, the determination of a mesenchymal stem cell into a preadipocyte, second, its conversion into a mature fat cell. In cloned cell lines adipose conversion depends on at least one postconfluent mitosis possibly induced by insulin-like growth factors or by as yet unknown mitogens. In addition growth hormone, glucocorticoids, and insulin are needed for conversion to take place. The adipose conversion of preadipocytes originating from the stromal vascular fraction of adipose tissue does not depend on postconfluent mitoses and needs only insulin and glucocorticoid hormones in physiological concentrations. However, the ability to undergo adipose conversion is not stable in these cells, but gets lost after repeated subcultures or seeding at low densities. In addition to stimulating hormones an increasing number of factors inhibiting the conversion process have also been detected, the physiological function of which remains unclear at the moment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01727475
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Angeborene nichtsphärocytäre hämolytische Anämie bei familiärem Mangel an DPNH- und TPNH-abhängiger Glutathionreduktase der Erythrocyten (1965)
    Springer
    Journal of molecular medicine 43 (1965), S. 427-429 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A case of a 16-year-old boy with a congenital non-spherocytic haemolytic anaemia is reported whose erythrocytes were deficient in both DPNH- and TPNH-Glutathione-Oxido-Reductase (GSSG-Reductase). In the erythrocytes of one of his brothers with no signs of haemolytic anaemia we found a smaller decrease of the DPNH-GSSG-Reductase, whereas two healthy brothers demonstrated a normal DPNH, GSSG-Reductase. The TPNH-GSSG-Reductase was decreased in all brothers. Among the examined family members the patient showed the most extensive decrease of the DPNH-GSSG-Reductase. It is suggested that development of this particular type of haemolytic anaemia is determined by the degree of DPNH-GSSG-Reductase deficiency.
    Notes: Zusammenfassung In den Erythrocyten eines 16jährigen Jungen mit einer angeborenen nichtsphärocytären hämolytischen Anämie wurde eine Aktivitätsminderung der DPNH- und TPNH-abhängigen Glutathionreduktase festgestellt. Drei klinisch gesunde Brüder zeigten ebenfalls eine Aktivitätsminderung der TPNH-abhängigen Glutathionreduktase, während nur bei einem dieser Geschwister gleichzeitig ein Mangel an DPNH-abhängiger Glutathionreduktase bestand. Da die Aktivitätsminderung der DPNH-abhängigen Glutathionreduktase bei dem Patienten am stärksten war, wird angenommen, daß das Ausmaß dieses Enzymdefektes für das Auftreten der hämolytischen Anämie von Bedeutung ist.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01483847
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Plasma digoxin levels in anuric patients and normal subjects taking digitoxin (1977)
    Springer
    Journal of molecular medicine 55 (1977), S. 245-246 
    Details
    ISSN: 1432-1440
    Keywords: Herzglykoside ; Digitoxin ; Digoxin ; Urämie ; Dialyse ; Radioimmunoassay ; Cardiac glycosides ; Digitoxin ; Digoxin ; Uremia ; Dialysis ; Radioimmunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Plasma digoxin suspected to be elevated in anuric patients taking digitoxin was determined by radioimmunoassay in 15 anuric patients and 15 normal persons subjected to 0.1 mg digitoxin therapy per day. All plasma digoxin values from the anuric patients and the normal subjects were far below the lower limit of the therapeutic range of plasma digoxin. There existed no difference between the digoxin values determined in anuric patients and subjects with normal renal function; in both groups there was a scatter of digoxin values about the cross reaction line between digitoxin and digoxin antibody. It is concluded from the results that digoxin retention in anuric patients taking digitoxin plays an insignificant role; thus, the pharmacological effect is mediated by digitoxin itself.
    Notes: Zusammenfassung Um zu klären, ob eine Retention von Digoxin bei anurischen Patienten, die Digitoxin einnehmen, stattfindet, wurde bei 15 anurischen Dialysepatienten und 15 nierengesunden Versuchspersonen, die 0,1 mg Digitoxin täglich einnahmen, das Plasmadigoxin mittels Radioimmunoassay bestimmt. Alle „Plasmadigoxinwerte“ der anurischen Patienten und der normalen Kontrollpersonen lagen weit unterhalb der unteren Grenze des therapeutischen Bereichs des Plasmadigoxins. Es fand sich kein Unterschied zwischen den Digoxinwerten, die bei den anurischen Patienten und bei den Versuchspersonen mit normaler Nierenfunktion gefunden wurden. Bei beiden Gruppen lagen die Digoxinwerte im Bereich der Kreuzreaktion zwischen Digitoxin und Digoxin-Antikörper. Aus den Ergebnissen wird die Schlußfolgerung gezogen, daß eine Digoxin-Retention bei anurischen Patienten, die Digitoxin einnehmen, eine untergeordnete Rolle spielt und daher der pharmakologische Effekt durch Digitoxin selbst hervorgerufen wird.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01487719
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...