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  • 1
    Electronic Resource
    Electronic Resource
    Bakteriologisch-hygienische Probleme bei der Hämodialyse (1971)
    Springer
    Journal of molecular medicine 49 (1971), S. 1092-1093 
    Details
    ISSN: 1432-1440
    Keywords: Artificial kidneys ; disinfection ; bacterial filters ; hemodialysis ; bacterial filtration of dialysate ; Künstliche Niere ; Desinfektion ; Bakterienfilter ; Haemodialyse ; Dialysatentkeimung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Eigene bakteriologische Befunde am Dialysat verschiedener Haemodialysegeräte und Literaturmitteilungen werden zum Anlaß genommen, die Wirkung verschiedener Methoden zur Entkeimung des Dialysates zu untersuchen. Daraus ergaben sich folgende Empfehlungen zur Erzielung keimfreier Dialysate: 1. Reinigung der Geräte mit geeigneten Desinfektionsmitteln unmittelbar vor jeder Dialyse. 2. Ausschließliche Benutzung von keimfreiem Wasser an den Geräten. 3. Einschalten von Bakterienfiltern in den Kreislauf der Dialyseflüssigkeit.
    Notes: Summary Bacteriologic studies were performed on the dialysate of artificial kidneys and the effectiveness of various methods of elimination of bacteria from the water system of the dialysers were evaluated. The following recommendations are given to obtain best bacteriologic results: 1. the dialysers are to be properly disinfected shortly before each dialysis; 2. the water used to operate the dialysers has to be cleaned from bacteria by means of bacterial filters; 3. the dialysate should pass another bacterial filter just before it enters the compartment of coil kidneys or the Kiil-Dialyser.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01732922
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  • 2
    Electronic Resource
    Electronic Resource
    Bestimmung des Knochenmineralgehaltes mit Hilfe der Photonenabsorptionstechnik bei renaler Osteopathie (1977)
    Springer
    Journal of molecular medicine 55 (1977), S. 1005-1011 
    Details
    ISSN: 1432-1440
    Keywords: Bone mineral analyzer ; Bone mineral analysis in renal osteodystrophy ; Bone demineralization in renal failure ; Photon absorptiometry in renal osteodystrophy ; Bone densitometry in renal osteopathy ; Knochenmineralanalysator ; Knochenmineralgehalt bei renaler Osteopathie ; Photonen-Absorptionstechnik
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe der Photonenabsorptionstechnik nach dem Verfahren von Cameron und Sorenson wurde die Knochendichte bei 58 Patienten im Prädialysestadium und 58 Patienten im Dauerdialyse-Programm bestimmt. Zusätzlich untersuchten wir den therapeutischen Effekt der Vitamin D3-Substitution bzw. der Verabreichung von 5,6-trans-25-OH-Vitamin D3 auf den Knochenmineralgehalt. Wir fanden eine Korrelation sowohl zwischen der Knochendichte und der Dauer der chronischen Niereninsuffizienz bzw. der Hämodialysebehandlung als auch der Höhe des Serum-Parathormonspiegels. Dagegen bestand keine Korrelation zwischen Knochendichte und Höhe des Serum-Calciumspiegels. Unter 14monatiger Behandlung mit Vitamin D3 bzw. 5,6-trans-25-OH-Vitamin D3 trat eine Zunahme der Knochendichte auf. Die Photonenabsorptionstechnik erwies sich als eine einfach durchführbare und gut reproduzierbare Methode zur Bestimmung des Knochenmine-ralgehaltes.
    Notes: Summary In 58 patients with end-stage renal failure before commencement of regular hemodialyses treatment (RTD) and 58 patients under RTD bone mineral content (BMC) was determined by the use of the photon absorptiometry. Further the effect of a treatment with vitamin D3 and 5,6-trans-25-OH-vitamin D3 on BMC was studied. There existed a negative correlation between the duration of chronic renal failure or of RDT as well as of the serum parathryoid hormone level to BMC. No correlation was found between BMC and serum calcium level. During a 14 months-course treatment with vitamin D3 or 5,6-trans-25-OH-vitamin D3 BMC increased. The method of photon absorptiometry presented itself as an easy and well reproducible tecnique for routine examinations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01488187
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  • 3
    Electronic Resource
    Electronic Resource
    Transmembrane ferricyanide reduction by cells of the yeastSaccharomyces cerevisiae (1982)
    Springer
    Journal of bioenergetics and biomembranes 14 (1982), S. 191-205 
    Details
    ISSN: 1573-6881
    Keywords: Plasmalemma redox ; transmembrane dehydrogenase ; proton transport
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Both respiratory-competent and respiratory-deficient yeast cells reduce external ferricyanide. The reduction is stimulated by ethanol and inhibited by the alcohol dehydrogenase inhibitor, pyrazole. The reduction of ferricyanide is not inhibited by inhibitors of mitochondrial or microsomal ferricyanide reduction. Cells in exponential-phase growth show a much higher rate of ferricyanide reduction. The reduction of ferricyanide is accompanied by increased release of protons by the yeast cells. We propose that the ferricyanide reduction is carried out by a transmembrane NADH dehydrogenase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00745020
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  • 4
    Electronic Resource
    Electronic Resource
    A transmembranous NADH-dehydrogenase in human erythrocyte membranes (1984)
    Springer
    Journal of bioenergetics and biomembranes 16 (1984), S. 517-533 
    Details
    ISSN: 1573-6881
    Keywords: Transmembranous NADH-dehydrogenase ; enzyme kinetics ; human erythrocyte ; plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Evidence is presented for a transmembranous NADH-dehydrogenase in human erythrocyte plasma membrane. We suggest that this enzyme is responsible for the ferricyanide reduction by intact cells. This NADH-dehydrogenase is distinctly different from the NADH-cytochromeb 5 reductase on the cytoplasmic side of the membrane. Pretreatment of erythrocytes with the nonpenetrating inhibitor diazobenzene sulfonate (DABS) results in a 35% loss of NADH-ferricyanide reductase activity in the isolated plasma membrane. Since NADH and ferricyanide are both impermeable, the transmembrane enzyme can only be assayed in open membrane sheets with both surfaces exposed, and not in closed vesicles. The transmembrane dehydrogenase has affinity constants of 90 µM for NADH and 125 µM for ferricyanide. It is inhibited byp-chloromercuribenzoate, bathophenanthroline sulfonate, and chlorpromazine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743243
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  • 5
    Electronic Resource
    Electronic Resource
    Properties of a transplasma membrane electron transport system in HeLa cells (1984)
    Springer
    Journal of bioenergetics and biomembranes 16 (1984), S. 583-595 
    Details
    ISSN: 1573-6881
    Keywords: Plasma membrane ; electron transport ; proton excretion ; growth factors ; ferricyanide reduction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract A transmembrane electron transport system has been studied in HeLa cells using an external impermeable oxidant, ferricyanide. Reduction of ferricyanide by HeLa cells shows biphasic kinetics with a rate up to 500 nmoles/min/g w.w. (wet weight) for the fast phase and half of this rate for the slow phase. The apparentK m is 0.125 mM for the fast rate and 0.24 mM for the slow rate. The rate of reduction is proportional to cell concentration. Inhibition of the rate by glycolysis inhibitors indicates the reduction is dependent on glycolysis, which contributes the cytoplasmic electron donor NADH. Ferricyanide reduction is shown to take place on the outside of cells for it is affected by external pH and agents which react with the external surface. Ferricyanide reduction is accompanied by proton release from the cells. For each mole of ferricyanide reduced, 2.3 moles of protons are released. It is, therefore, concluded that a transmembrane redox system in HeLa cells is coupled to proton gradient generation across the membrane. We propose that this redox system may be an energy source for control of membrane function in HeLa cells. The promotion of cell growth by ferricyanide (0.33–0.1 mM), which can partially replace serum as a growth factor, strongly supports this hypothesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743247
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  • 6
    Electronic Resource
    Electronic Resource
    Transplasmalemma electron transport is changed in simian virus 40 transformed liver cells (1986)
    Springer
    Journal of bioenergetics and biomembranes 18 (1986), S. 471-485 
    Details
    ISSN: 1573-6881
    Keywords: Plasma membrane ; pyridine nucleotide oxidation ; temperature sensitive SV40 ; liver cells ; transmembrane electron transport ; cell transformation ; enzyme kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Transplasma membrane electron transport activity by fetal rat liver cells (RLA209-15) infected with a temperature-sensitive strain of SV40 has been measured with cells grown at the restrictive temperature (40°C) and permissive temperature (33°C). The transformed cells grown at 33°C had only one-half the rate of external ferricyanide reduction as the nontransformed cells held at 40°C. Both theK m andV max for ferricyanide reduction were changed in the transformed state. The change inV max can be based on a decrease of NADH in the transformed cells. The change in rate with ferricyanide does not depend on change in surface charge. Reduction of external ferricyanide was accompanied by release of protons from the cells. The ratio of protons released to ferricyanide reduced was higher in the transformed cells than in the non-transformed cells. Since the transplasma membrane electron transport has been shown to stimulate cell growth under limiting serum, the changes in the plasma membrane electron transport and proton release in transformed cells may relate to modification of growth control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743145
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  • 7
    Electronic Resource
    Electronic Resource
    Transformation with SV40 virus prevents retinoic acid inhibition of plasma membrane NADH diferric transferrin reductase in rat liver cells (1988)
    Springer
    Journal of bioenergetics and biomembranes 20 (1988), S. 383-391 
    Details
    ISSN: 1573-6881
    Keywords: Transplasma membrane electron transport ; plasma membranes ; retinoic acid ; cell differentiation ; SV40 transformation ; adriamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40°C. When the cells are in the transformed state (grown at the permissive 33°C temperature), retinoic acid does not inhibit the diferric transferrin reduction. Inhibition of activity of nontransformed cells is specific for retinoic acid with only slight inhibition by retinol and retinyl acetate at higher concentrations. Isolated rat liver plasma membrane NADH diferric transferrin reductase is also inhibited by retinoic acid. The effect of transformation with SV40 virus to decrease susceptibility to retinoic acid inhibition stands in contrast to much greater adriamycin inhibition of diferric transferrin reduction in the transformed cells than in nontransformed cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769639
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  • 8
    Electronic Resource
    Electronic Resource
    Insulin control of a transplasma membrane NADH dehydrogenase in erythrocyte membranes (1982)
    Springer
    Journal of bioenergetics and biomembranes 14 (1982), S. 425-433 
    Details
    ISSN: 1573-6881
    Keywords: Plasma membrane ; NADH dehydroganase ; erythrocyte membrane ; insulin ; transplasma membrane dehydrogenase ; human erythrocyte ; porcine erythrocyte ; hormone action
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract A study of NADH ferricyanide reductase activity in oriented vesicles or open ghosts of human and porcine erythrocytes shows that the dehydrogenase activity can have three types of orientation in the membrane. There is activity which responds only to acceptors and NADH exclusively on the inside face, or exclusively on the outer surface. There is also activity which requires exposure of both sides of the membrane and thus is transmembranous. The transmembrane activity is inhibited by insulin, whereas the internal and external enzymes do not respond to insulin. The transmembrane dehydrogenase can be a basis for proton transport in the plasma membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00743068
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  • 9
    Electronic Resource
    Electronic Resource
    Inhibition of plasma membrane NADH dehydrogenase by adriamycin and related anthracycline antibiotics (1984)
    Springer
    Journal of bioenergetics and biomembranes 16 (1984), S. 209-221 
    Details
    ISSN: 1573-6881
    Keywords: Plasma membrane ; NADH dehydrogenase ; antitumor drugs ; adriamycin ; anthracycline
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract Doxorubicin (adriamycin) is cytotoxic to cells, but the biochemical basis for this effect is unknown, although intercalation with DNA has been proposed. This study suggests that the cytotoxicity of this drug may be due to inhibition of the plasma membrane redox system, which is involved in the control of cellular growth. Concentrations between 10−6–10−7 M adriamycin inhibit plasma membrane redox reactions 〉50%. AD32, a form of adriamycin which does not intercalate with DNA, but is cytotoxic, also inhibits the plasma membrane redox system. Thus, the cytotoxic effects of adriamycin, which limit its use as a drug, may be based on the inhibition of a transplasma membrane dehydrogenase involved in a plasma membrane redox system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00751050
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  • 10
    Electronic Resource
    Electronic Resource
    Quantitative Infrarot-Messungen an Polyvinylchlorid-Weichmacher-Systemen (1959)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 170 (1959), S. 155-166 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Zusammenfassung Methoden zur infrarotspektroskopischen Untersuchung von weichgemachten Polyvinylchlorid-(PVC-)Folien werden beschrieben. Die Ergebnisse von Intensitätsmessungen an Folien verschiedener PVC-Sorten und an Di-alkylphthalsäureestern werden mitgeteilt und diskutiert. Es wird gezeigt, daß in PVC-Phthalsäureester-Systemen Schlüsselfrequenzen (für PVC1430 cm−1, für Phthalate 1728 cm. −1) vorhanden sind, die in einem technisch interessierenden Konzentrationsbereich dem Lambert-Beerschen Gesetz folgen und daher zur Konzentrationsbestimmung von Phthalsäureestern in PVC-Folien geeignet sind.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00448566
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