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Extent and nature of extracellular organic production by the marine coccolithophorid Hymenomonas carterae (1989)

Marlowe, I. T. ; Rogers, L. J. ; Smith, A. J.

Springer

Marine biology 100 (1989), S. 381-391

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ISSN: 1432-1793

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Production of extracellular dissolved organic substances by cultures of the marine coccolithophorid Hymenomonas carterae SMBA 254 was investigated by the 14C-tracer technique. In 4 h incubations, extracellular 14C production represented 20 to 64% of 14C incorporation into cell material for cultures nearing, or during stationary growth; for rapidly growing cultures with relatively low cell densities the 14C-production was only 4 to 10% of incorporation. Intra-and extra-cellular material was fractionated by ion-exchange membrane electrodialysis. Ionic compounds accounted for 40 to 60% of extracellular material; in contrast only 16 to 27% of intracelular metabolites were in this category. Of a range of methods which were investigated, separation of compounds by gel-filtration in conjunction with two-dimensional thin-layer chromatography proved most effective. Up to 80% of extracellular material was of less than 1 800 mol. wt and included carbohydrates and amino acids, with glutamic acid predominating. The intracellular material was more heterogeneous, with a substantially greater proportion of high molecular weight material. The quite distinct compositions show clearly that extracellular production observed in these experiments arose through excretion and was not attributable to cell lysis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00391154

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Molecular stratigraphy: a new tool for climatic assessment (1986)

Brassell, S. C. ; Eglinton, G. ; Marlowe, I. T. ; [et al.]

[s.l.] : Nature Publishing Group

Nature 320 (1986), S. 129-133

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Variations in sea-surface temperatures over the past 500,000 years are inferred from the relative abundance behaviour of two organic compounds, C37 alkenones over the upper 8 metres of a sediment core from the eastern equatorial Atlantic. This molecular record, ascribed to contributions ...