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  • 1
    Electronic Resource
    Electronic Resource
    Progression of cellular repopulation and collagen synthesis in fresh-frozen allograft tendons (1996)
    Oxford, UK : Blackwell Science
    Wound repair and regeneration 4 (1996), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The progression of cellular repopulation and collagen synthesis in fresh-frozen rat patellar tendon allografts was investigated by means of indirect immunofluorescence histologic analysis and electron microscopic techniques for 8 weeks after transplantation. In each of 10 procedures, the medial half of the patellar tendon with a tibial bone block was harvested from a Wistar rat and transplanted into a corresponding defect in the medial patellar tendon of a Lewis rat. Actin filaments in the repopulating cells and newly synthesized collagen fibrils in the graft were identified with rhodamine-phalloidin stain and with a polyclonal antibody against type III collagen aminopro-peptide. On the first day after transplantation, no specific fluorescence was detected in the graft. One week later, specific labeling for fibrillar-actin (F-actin) and type III collagen aminopropeptide was detected in an area extending from the adjacent granulation tissue into the proximal end of the graft. F-actin and type III collagen aminopropeptide were aligned along the longitudinal axis of the graft and extended from the proximal suture site toward the distal portion. Two weeks after transplantation, fibrillar labeling for F-actin and type III collagen amino-propeptide showed that remodeling had extended to the midportion of the graft. Labeling throughout the entire graft was detected 4 weeks after transplantation. During the entire remodeling process, the repopulated fibroblasts consistently retained their elongated shape and their alignment with the longitudinal axis of the graft. The cells developed well-organized actin bundles at their peripheries, which identified them as having a myofibroblast phenotype. Immunofluorescence detection for type III collagen aminopropeptide also showed consistent alignment parallel to the longitudinal axis of the graft and a fibrillar arrangement. Electron microscopy revealed thinner collagen fibrils in the vicinity of the fibroblasts, which were aligned in the direction of the actin bundles. These results indicate that, during the early remodeling phase, collagen synthesis and deposition in the graft proceeds while the original alignment of the graft matrix is preserved. The close association between the alignment of actin bundles in repopulated “myofibroblastic” cells and that of newly synthesized collagen fibrils along the lines of the graft matrix may represent evidence of force transmission between the actin cytoskeleton and the linking extracellular matrix in vivo.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1524-475X.1996.40115.x
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  • 2
    Electronic Resource
    Electronic Resource
    P-glycoprotein expression in soft-tissue sarcomas (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 352-356 
    Details
    ISSN: 1432-1335
    Keywords: P-glycoprotein ; Soft-tissue sarcoma ; Multidrug resistance ; Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Multidrug resistance (MDR) is an important problem in chemotheraphy for neoplastic disease. In humans, MDR is mainly mediated by P-glycoprotein (P-gp), a product of theMDR1 gene, which acts as a transmembrane protein pump and eliminates chemotherapeutic agents from the cells. Expression of P-gp was immunohistochemically studied by using two monoclonal antibodies, JSB-1 and C-219, on paraffin-embedded sections from 55 patients with soft-tissue sarcoma. The histological diagnosis of tumors was malignant fibrous histiocytoma in 24 cases, liposarcoma in 9, synovial sarcoma in 7, malignant neurogenic tumors in 6, leiomyosarcoma in 5, others in 4. The histological grade was determined on the basis of criteria previously proposed by us. Out of 55 cases, 34 (62%) were positive for P-gp expression. There was a significant difference in P-gp expression between high-grade (90%) and intermediate and low-grade tumors (46%) (P〈0.005). Tumors expressing P-gp had a less favorable prognosis than P-gp-negative tumors in the high- and intermediate-grade tumors. The current study demonstrated that the estimation of P-gp expression could be used to select appropriate therapeutic modalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01438312
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  • 3
    Electronic Resource
    Electronic Resource
    P-glycoprotein expression in soft-tissue sarcomas (1997)
    Springer
    Journal of cancer research and clinical oncology 123 (1997), S. 352-356 
    Details
    ISSN: 1432-1335
    Keywords: Key words P-glycoprotein  ;  Soft-tissue sarcoma  ; Multidrug resistance  ;  Prognosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Multidrug resistance (MDR) is an important problem in chemotheraphy for neoplastic disease. In humans, MDR is mainly mediated by P-glycoprotein (P-gp), a product of the MDR1 gene, which acts as a transmembrane protein pump and eliminates chemotherapeutic agents from the cells. Expression of P-gp was immunohistochemically studied by using two monoclonal antibodies, JSB-1 and C-219, on paraffin-embedded sections from 55 patients with soft-tissue sarcoma. The histological diagnosis of tumors was malignant fibrous histiocytoma in 24 cases, liposarcoma in 9, synovial sarcoma in 7, malignant neurogenic tumors in 6, leiomyosarcoma in 5, others in 4. The histological grade was determined on the basis of criteria previously proposed by us. Out of 55 cases, 34 (62 %) were positive for P-gp expression. There was a significant difference in P-gp expression between high-grade (90 %) and intermediate and low-grade tumors (46 %) (P 〈 0.005). Tumors expressing P-gp had a less favorable prognosis than P-gp-negative tumors in the high- and intermediate-grade tumors. The current study demonstrated that the estimation of P-gp expression could be used to select appropriate therapeutic modalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01438312
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  • 4
    Electronic Resource
    Electronic Resource
    Association of the human NPPS gene with ossification of the posterior longitudinal ligament of the spine (OPLL) (1999)
    Springer
    Human genetics 〈Berlin〉 104 (1999), S. 492-497 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract OPLL (ossification of the posterior longitudinal ligament of the spine) is a common form of human myelopathy with a prevalence of as much as 4% in a variety of ethnic groups. To clarify the genetic factors that predispose to OPLL, we have studied ttw (tiptoe walking), a mouse model that presents ectopic ossification of the spinal ligaments similar to OPLL and have found that the ttw phenotype is caused by the nonsense mutation of the gene encoding nucleotide pyrophosphatase (NPPS), a membrane-bound glycoprotein thought to produce inorganic pyrophosphate, a major inhibitor of calcification and mineralization. To investigate a possible role of NPPS in the etiology of OPLL, we have examined its genetic variations in OPLL patients. A total of 323 OPLL patients was screened by means of polymerase chain reaction/single-strand conformation polymorphism analysis covering all the exons and their surrounding introns, plus about 1.5-kb of the promoter region. We identified ten nucleotide variations in the NPPS gene; five of the alterations caused amino-acid substitutions, and two of them were found specifically in OPLL patients. Subsequently, we performed an association study using these variations and found a significant association of an allele, viz., a deletion of T at a position 11 nucleotides upstream from the splice acceptor site of intron 20 (IVS20–11delT), with OPLL; the proportion of the individuals having this deletion was significantly higher (P = 0.0029) in OPLL patients than in controls, indicating that those who have this variation may be more susceptible to the abnormal ossification of the spinal ligaments. Thus, our study suggests that NPPS plays an important role in the etiology of human OPLL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050993
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  • 5
    Electronic Resource
    Electronic Resource
    Pathology of femoral head collapse following transtrochanteric rotational osteotomy for osteonecrosis (2000)
    Springer
    Archives of orthopaedic and trauma surgery 120 (2000), S. 489-492 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We investigated the pathology of femoral head collapse following transtrochanteric anterior rotational osteotomy. Six femoral heads were obtained during total hip arthroplasty some 2–12 years after osteotomy. In all cases, the preoperatively necrotic lesions exhibited mostly osteonecrosis with accumulation of bone marrow cell debris and trabecular bone with empty lacunae, although repair tissue such as granulation tissue and appositional bone formation were observed in limited areas in some cases. In the transposed intact articular surface of the femoral head, osteoarthritic changes such as fissure penetration to the subchondral bone and osteophyte formation were commonly observed. In newly created subchondral areas at weight-bearing sites, trabecular thickness and the number of trabecular bones had decreased, with few osteoblasts, osteoclasts, and osteocytes being present, resulting in a coarse lamellar structure of the trabecular bone. These findings suggest that transposed areas in cases of failure consist mostly of low-turnover osteoporotic lesions which could cause collapse of the femoral head.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004020000144
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  • 6
    Electronic Resource
    Electronic Resource
    Stem length and canal filling in uncemented custom-made total hip arthroplasty (1999)
    Springer
    International orthopaedics 23 (1999), S. 219-223 
    Details
    ISSN: 1432-5195
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé. Pour étudier la relation existant entre la longueur de la tige et le remplissage du canal dans une arthroplastie totale de la hanche effectuée sur mesure, nous avons réexaminé 60 éléments fémoraux de deux longueurs différentes: 125 mm (groupe A) et 100 mm (groupe B). Que ce soit l’áge, le genre, la hauteur, le poids du corps, l’indice d’érasement du canal ou l’angle de courbure du fémur, il n’existait pas de différences statistiques entre les deux groupes. Alors qu’il n’y avait pas de différences statistiques post-opératoires entre les deux groupes dans le remplissage du canal proximal, il y avait par contre une différence significative dans le remplissage du canal distal (75.5% par rapport á 85.8% sur une vue antéropostérieure et 76.0% par rapport á 82.5% sur une vue latérale, P〈0.001). Le remplissage du canal distal se rattachait inversement avec le rapport de la portion proximale et de la portion distale de la courbure de la tige sur la vue latérale (rapport de la courbure latérale, P=0.002). On en conclut que le remplissage supérieur aux niveaux proximaux et distaux peut étre obtenu en utilisant des éléments sur mesure de 100 mm avec un faible rapport de la courbure latérale.
    Notes: Abstract  We reviewed 60 custom-made femoral components of two different lengths : 125 mm (group A) and 100 mm (group B), in order to investigate the relationship between stem length and canal filling in uncemented custom-made total hip arthroplasty. There were no statistical differences between the two groups in age, gender, height, body weight, canal flare index, or bowing angle of the femur. Postoperatively there was no statistical difference between the two groups in the proximal canal filling, but significant difference in the distal canal filling (75.5% vs 85.8% on the anteroposterior view and 76.0% vs 82.5% in the lateral view, P〈0.001). The distal canal filling inversely correlated with the ratio of the proximal portion and the distal portion of the stem curvature on the lateral view (lateral curve ratio of the stem, P=0.002). We conclude that superior filling at both the proximal and the distal levels can be obtained by using 100-mm custom made components with a small lateral curve ratio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002640050355
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  • 7
    Electronic Resource
    Electronic Resource
    Florid periosteal reaction and focal fibrocartilaginous dysplasia (1998)
    Springer
    Skeletal radiology 27 (1998), S. 646-649 
    Details
    ISSN: 1432-2161
    Keywords: Key words Focal fibrocartilaginous dysplasia ; Periosteal reaction ; Tibia vara
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Focal fibrocartilaginous dysplasia (FFCD) is a rare condition causing tibia vara in childhood. It is characterized by progressive tibia vara in young children with a characteristic radiographic lesion. This paper is thought to be the first to describe FFCD exhibiting florid periosteal reaction at the time of presentation with a subtle faint osteolytic lesion in the diametaphysis of the proximal tibia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050452
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  • 8
    Electronic Resource
    Electronic Resource
    MR arthrography of elbow: evaluation of the ulnar collateral ligament of elbow (1996)
    Springer
    Skeletal radiology 25 (1996), S. 629-634 
    Details
    ISSN: 1432-2161
    Keywords: Key words Elbow ; Ulnar collateral ligament ; Magnetic resonance imaging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective. The purpose of this study was to evaluate ulnar collateral ligament (UCL) injury of the elbow in throwing athletes by MRI and MR arthrography. Design. Ten elbows of throwing athletes were examined on both plain MRI and MR saline arthrography and the injuries subsequently surgically proven. Spin-echo (SE) T1-weighted and fast SE T2-weighted coronal images were obtained. Results. The UCL was unclear in all ten cases on T1-weighted MRI. In five cases an avulsion fracture was also found on T1-weighted MRI. On T2-weighted MRI, abnormal high-intensity areas were identified in or around the UCL. On T2-weighted MR arthrography images, extracapsular high-intensity areas, which represent extracapsular leakage, were found in four of five cases with avulsion fracture. At surgery, all these four cases showed avulsion fractures with instability; the other case had a fracture but it was stable and adherent to the humerus. On T2-weighted MR arthrography images, an extracapsular high-intensity area was found in one of the five cases without avulsion fracture. At surgery this patient had a complete tear of the UCL itself. Conclusion. MR arthrography provided additional information for evaluating the degree of UCL injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002560050149
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  • 9
    Electronic Resource
    Electronic Resource
    A technique of percutaneous multidrilling osteotomy for limb lengthening and deformity correction (2000)
    Springer
    Journal of orthopaedic science 5 (2000), S. 104-107 
    Details
    ISSN: 1436-2023
    Keywords: Key words: limb lengthening, distraction osteogenesis, multidrilling osteotomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: We have recently developed a technique of per-cutaneous multidrilling osteotomy for limb lengthening and deformity correction. The bone is drilled percutaneously, using a special drill guide, and osteotomy is accomplished by connecting the multiple drill holes with a small chisel. The bone segments are subjected to slow progressive distraction with an external fixation device. We have lengthened 33 limbs in 22 patients with congenital or post-traumatic limb shortening and/or bone deformities. All the patients underwent the proposed lengthening and/or correction of the bone deformities through a single-treatment procedure. None of the lengthened segments resulted in nonunion. This technique can prevent undesirable bone cracks and preserve soft tissue around the osteotomy site, and is also applicable to other fields of orthopedic surgery.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007760050136
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  • 10
    Electronic Resource
    Electronic Resource
    A common FGFR3 mutation functions as a diagnostic marker for achondroplasia-group disorders in the Japanese population (1996)
    Springer
    Journal of orthopaedic science 1 (1996), S. 130-135 
    Details
    ISSN: 1436-2023
    Keywords: achondroplasia ; mutation ; fibroblast growth factor receptor 3 ; Japanese population
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Recent DNA studies performed by several groups have detected mutations of the gene encoding fibroblast growth factor receptor 3 (FGFR3) in patients with achondroplasia-group disorders, including achondroplasia (ACH), hypochondroplasia (HCH), and thanatophoric dysplasia (TD). For this study, we analyzed theFGFR3 gene in 31 Japanese patients with typical ACH, four with HCH, three with a condition intermediate between ACH and HCH (ACH/HCH-intermediate), and one with TD. Of the 31 typical ACH patients, 29 showed a G1138 to A transition and the other two a G1138 to C transversion, both resulting in a common Gly380Arg substitution in the transmembrane domain of FGFR3. The one TD and the four HCH patients did not display any mutations in the transmembrane domain of FGFR3. Of the three ACH/HCH-intermediate cases, one patient showed the Gly380Arg substitution and one did not, and further analysis of the second patient revealed the presence of Asn540Lys substitution. The first patient was, therefore, genotypically diagnosed as ACH and the second as HCH. Peripheral blood leukocyte DNA analysis in the remaining ACH/HCH-intermediate patient indicated an unequal ratio of mutant to normal PCR products, possibly representing a somatic mosaic for the Gly380Arg mutation. Analysis of the common FGFR3 mutation thus appears to help in the molecular diagnosis of patients with achondroplasia-group disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02348804
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