Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Alteration of cell fate by ectopic expression of Drosophila glial cells missing in non-neural cells (1998)
    Springer
    Development genes and evolution 208 (1998), S. 578-585 
    Details
    ISSN: 1432-041X
    Keywords: Key words Glial cells missing ; Drosophila melanogaster ; GAL4-UAS system ; Ectopic expression ; Cell fate determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The glial cells missing (gcm) gene encodes an essential transcription factor that converts neuronal precursor cells to glial fate in the Drosophila nervous system. In this study, we tested effects of gcm ectopic expression on fate of non-neural cells. When gcm expression was continuously induced in epidermal cells from around stage 9, these cells started to exhibit mesenchymal cell morphology at stage 13, which was preceded by the onset of expression of Repo, a glial marker. The morphological change was coincident with loss of expression of an epidermal cell-adhesion molecule. In addition to the epidermis, fate of mesodermal cells was also affected by gcm ectopic expression. These findings suggest that gcm can convert gene expression and cell morphology even outside the neuroectoderm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004270050217
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Expression of proto-oncogene products during drug-induced differentiation of a neuroblastoma cell line SK-N-DZ (1989)
    Springer
    Acta neuropathologica 79 (1989), S. 217-221 
    Details
    ISSN: 1432-0533
    Keywords: Neuroblastoma ; Differentiation ; Retinoic acid ; Proto-oncogene ; Tyrosine phosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Four human neuroblastoma cell lines exhibited differences in their ability to differentiate into neuron-like cells in response to three different treatments, serum deprivation, or additions of dibutyryl cyclic-AMP or retinoic acid. Expression of N-myc gene product was reduced in neuroblastoma cell line SK-N-DZ differentiated by retinoic acid as compared with untreated cells. On the contrary, expression of c-src gene product, pp60c-src, was considerably enhanced in differentiated SK-N-DZ cells. Tyrosine phosphorylation of several cellular proteins was found to be enhanced in differentiated cells. Alteration in expression of these proto-oncogene products might be important in the differentiation of neuroblastoma cells into neuron-like cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294382
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Alginate degradation by bacteria isolated from the gut of sea urchins and abalones (1995)
    Springer
    Microbial ecology 30 (1995), S. 193-202 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Degradation of alginate and its constituents, polymannuronate (polyM) and polyguluronate (polyG), by gut bacteria isolated from sea urchins and abalones in the northern part of Japan, were investigated. Bacterial counts in the guts of sea urchin S. intermedius, were 105 to 108 CFU/g, and in abalone H. discus hannai, counts ranged from 106 to 109 CFU/g. More than 80% of total 600 isolates were found to have alginolytic activity. The alginolytic bacteria were predominantly fermentative, but some differences were observed in their substrate specificity as well as between the flora in the gut of sea urchins and the abalones. Seventy percent of the alginolytic bacteria from the sea urchins showed no degrading preference for polyM or polyG blocks, and were able to degrade both the substrates simultaneously. Most of the alginolytic bacteria (96.6%) from sea urchins belonged to the genus Vibrio. The majority of alginolytic bacteria (68.0% on average) from abalones only degraded polyG and they were predominantly non-motile fermenters. From these results, it appeared that a different type of association exists between alginolytic gut microflora and the marine algal feeders with respect to the level of contribution by bacteria to the host's digestion of alginate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172574
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A case of crush syndrome occurring during alcohol withdrawal: extended disturbance of consciousness with development of persistent Korsakoff's psychosis (1998)
    Springer
    Intensive care medicine 24 (1998), S. 1231-1231 
    Details
    ISSN: 1432-1238
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001340050751
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Formation of functional synapses in the adult cat red nucleus from the cerebrum following cross-innervation of forelimb flexor and extensor nerves (1982)
    Springer
    Experimental brain research 45 (1982), S. 13-18 
    Details
    ISSN: 1432-1106
    Keywords: Sprouting ; Red nucleus ; Cross innervation ; Unitary corticorubral EPSPs ; Cable properties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The effects of cross-innervation of peripheral flexor and extensor nerves on the time course and properties of red nucleus (RN) neurons were studied in adult cats. The time course of corticorubral unitary EPSPs was examined. In operated cats, RN neurons innervating upper spinal segments (C-cells) manifested corticorubral unitary EPSPs with shorter time-to-peak and larger amplitude than in normal cats. The mean amplitude of these EPSPs was 0.41 ± 0.11 mV; the mean time-to-peak was 1.14 ± 0.51 ms (n = 18). These values differ from normal cats (0.33 ± 0.09 mV, and 2.68 ± 0.61 ms, n = 22). RN neuron membrane properties were examined in cross-innervated cats. The main time constant was 5.2 ± 0.7 ms (n = 10), the shorter equalizing time constant, 0.69 ± 0.19 ms (n = 10), the input resistance, 2.5 ± 0.8 MΩ. These values were not significantly different from those of normal cats. The electrotonic length was 1.2 (n = 10), somewhat larger than in normal cats. The number of converging interposito-rubral synapses, estimated in eight cells from cross-innervated cats, ranged from 37 to 57. This was not significantly different from normal cats, and indicated that there is no large-scale degeneration of interpositorubral synapses after cross-innervation. These results suggest that sprouting and formation of functional synapses occur after cross-innervation of peripheral flexor and extensor nerves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235758
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Formation of functional synapses in the adult cat red nucleus from the cerebrum following cross-innervation of forelimb flexor and extensor nerves (1982)
    Springer
    Experimental brain research 45 (1982), S. 1-12 
    Details
    ISSN: 1432-1106
    Keywords: Sprouting ; Corticorubral synapse ; Red nucleus ; Cross-innervation ; Sprouting without degeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the effects of cross-innervating the peripheral forelimb flexor and extensor nerves of adult cats on the time course of corticorubral EPSPs. Red nucleus neurons were identified by antidromic invasion from C1 or L1 spinal segments as innervating the upper spinal segments (C-cells) or sending axons to the lumbosacral cord (L-cells). In C-cells, a fast-rising component, superimposed on the slow-rising corticorubral EPSPs induced by the cerebral sensorimotor cortex or the cerebral peduncle (CP) stimulation, was noted. The mean time-to-peak of this component in cross-innervated cats operated more than two months earlier was 1.9 ± 0.9 ms (n = 160), shorter than in normal cats (3.6 ± 1.4 ms, n = 100). The same value in cats cross-innervated less than two months before was 2.7 ± 1.0 ms (n = 53). The mean time-to-peak of CP-EPSPs from L-cells was 2.9 ± 0.9 ms (n = 115). The fast-rising component had a latency of 0.96 ± 0.19 ms (n = 122), and it was mediated by fibers with conduction velocities of less than 20 m/s. The projective area of the fast-rising component is organized somatotopically. Since it is more sensitive to membrane hyperpolarization than slow rising corticorubral EPSPs, it is mediated by synapses located more proximally than the corticorubral synapses of normal cats. The time course of facilitation by preceding cerebral peduncle stimulation of the nucleus interpositus (IP)-induced RN population responses was measured. It was characterized by a rapid, followed by a slower, rise time in the RN region where C-cells are concentrated. In contrast, the L-cell region was characterized by a slow rise time. In cats subjected to self-union of the peripheral flexor and extensor nerves, the majority of C-cells had CP-EPSPs with a time-to-peak within the normal range. Our results suggest that after cross-innervation, sprouting and formation of functional synapses occur on the proximal portion of the soma-dendritic membrane of red nucleus neurons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235757
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Simple and complex spike activities of purkinje cells during locomotion in the cerebellar vermal zones of decerebrate cats (1981)
    Springer
    Experimental brain research 41 (1981), S. 292-300 
    Details
    ISSN: 1432-1106
    Keywords: Purkinje cells ; Vermis ; Locomotion ; Cats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In walking cats decerebrated at the premammillary level, single neurone activity of Purkinje cells (P-cells) with long corticofugal axons was recorded in the cerebellar vermis. The P-cells (N = 145) were identified as they showed spontaneous simple and complex spikes and also antidromic activation from Deiters' nucleus. These P-cells were classified into 6 groups according to the receptive fields of the climbing fibre responses (CFRs) which were evoked by electrical stimulation in each limb at the radial and sciatic nerve bundles. One group designated as forelimb units received the CFRs from both forelimbs and from neither hindlimb. According to previous studies, this group of P-cells is thought to make inhibitory connections with Deiters neurones projecting to the ipsilateral cervicothoracic spinal cord. For the forelimb units, two types of discharge patterns for simple spikes were found in relation to limb movements during locomotion. Type I cells showed one peak in their firing rate in the late swing (E1) or early stance (E2) phase of the ipsilateral forelimb. Type II cells showed two peaks and two valleys during one step cycle: one peak was in the E1 phase, the other in the late stance (E3) or early swing (F) phase; each of the two valleys followed the peak. Complex spikes of the forelimb units occurred more frequently in the E1 phase than during the other phases. The increased activity of simple and complex spikes of the forelimb units in the E1 phase is suggested to have a functional significance in preparing the appropriate floor reaction forces that appear upon touchdown of the ipsilateral forelimb.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00238886
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Die cutan-allergische Reaktion auf 2,4-Dinitrochlorbenzol bei kindlichen Leukämien und soliden Tumoren (1973)
    Springer
    European journal of pediatrics 114 (1973), S. 143-154 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung An 40 Kindern mit akuten Leukämien und soliden Tumoren sowie 31 Kontrollpatienten wurde das Verhalten der cutan-allergischen Spätreaktion auf 2,4-Dinitrochlorbenzol (DNCB) und Tuberkulin untersucht. Dabei ergaben sich bei Verlaufsbeobachtungen der Hautreaktionsstärke signifikante Unterschiede zwischen den beiden Malignomgruppen und dem Kontrollkollektiv. Kinder mit Leukosen oder soliden Tumoren ließen sich weit weniger gut gegen DNCB sensibilisieren als Patienten ohne neoplastische Erkrankungen. Der negative Testausfall wird zum großen Teil durch die cystostatische bzw. Strahlentherapie erklärt. Da jedoch Leukosepatienten in der Remissionsphase trotz cytostatischer Therapie häufiger positive Cutanreaktionen zeigten als im akuten Schub, ferner bei Kindern mit neurogenen Geschwülsten signifikant weniger positive Hautreaktionen auszulösen waren als bei Kindern mit sarkomatösen Tumoren, müssen noch andere Faktoren Einfluß auf die immunologische Reaktionslage nehmen. Ein Plasmafaktor wird diskutiert.
    Notes: Abstract The delayed type of hypersensitivity on the skin was studied after application of 2,4-dinitrochlorbenzol (DNCB) or tuberculin to 40 children who had either acute leukemia or solid tumors. Thirty-one patients were used as a control group. There was a significant difference in the results between the neoplasm group and the control group. It proved more difficult to sensitize the leukemia and tumor groups with DNCB. The negative reaction is thought to be caused primarily by irradiation therapy or by cytostatic agents. However, on remission of leukemia a higher amount of positive skin reactions was observed in spite of the cytostatic therapy. Children suffering from primary neurogenic tumors also showed significantly less positive reactions than those who had sarcoma. Therefore other factors must exist that influence the immunologic reaction. A plasma factor is to be discussed in this study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440501
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Prognosis of intracranial germ cell tumours: Effectiveness of chemotherapy with cisplatin and etoposide (CDDP and VP-16) (1993)
    Springer
    Acta neurochirurgica 120 (1993), S. 111-117 
    Details
    ISSN: 0942-0940
    Keywords: Germ cell tumour ; chemotherapy ; CDDP ; VP-16
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A co-operative study for patients with intracranial germ cell tumours was performed to analyze their prognosis and the effectiveness of Cisplatin/Etoposide (CDDP/VP-16) chemotherapy. A total of 46 patients; 30 primary and 16 recurrent cases were registered from 15 participating neurosurgical institutions in Japan. Based on histological criteria and tumour markers, they were classified into three groups; germinoma, germinoma with syncytiotro-phoblastic giant cell (STGC), and non-germinomatous malignant tumour. Sixteen patients were treated with CDDP/VP-16 chemotherapy alone and the other 30 patients were treated by a combination of surgery and/or radiation in addition to chemotherapy. Eleven out of 13 patients (85%) with germinoma showed a complete (n=10) or partial (n=1) response to CDDP/VP-16 chemotherapy even if their tumours were recurrent and there was evidence of CSF dissemination. For the germinoma with STGC and non-germinomatous malignant tumour, a high response rate; 100% for the former and 78% for the latter, could also be achieved in both the primary and the recurrent cases except in those cases of immature teratoma. Their survival times were still different between them. Two-year survival was 50% in germinoma with STGC and 48% in non-germinoma, while it was 88% in germinoma cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02112027
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Crystallization and preliminary X-ray diffraction analysis of two lysinal derivatives of Achromobacter protease I (1996)
    Copenhagen : International Union of Crystallography (IUCr)
    Acta crystallographica 52 (1996), S. 1027-1029 
    Details
    ISSN: 1399-0047
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Notes: Two crystal forms of lysinal derivatives of Achromobacter protease I have been obtained. The first, modified by benzyloxycarbonyl-Val-lysinal crystallizes in the monoclinic space group P21 with unit-cell dimensions of a = 39.6, b = 71.2, c = 45.6 Å and β = 98.4°. The second, modified by benzyloxycarbonyl-Leu-Leu-lysinal crystallizes in the orthorhombic space group I222 (or I212121) with unit-cell dimensions of a = 98.7, b = 102.2 and c = 55.8 Å. The space groups and the unit-cell dimensions of the present two lysinal derivatives are different to those of the protease and TLCK- modified one. The space group of the protease is P1 with cell dimensions a = 39.53, b = 40.34, c = 43.92 Å, α = 114.81, β = 113.75 and γ = 74.00° and that of the TLCK-modified one is also P1 with cell dimensions of a = 37.30, b = 42.74, c = 48.02 Å, α = 120.10, β = 112.81 and γ = 68.54°. Diffraction to 1.9 Å resolution for the Val-lysinal modified crystal and to 2.2 Å resolution for the Leu-Leu-lysinal modified crystal has been observed using a rotating-anode X-ray generator. Full structure determinations of these lysinal-modified protease crystals may lead to an understanding of the molecular basis of enzyme–substrate interactions in the catalytic process of this protease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1107/S0907444996006920
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...