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  • 1
    Electronic Resource
    Electronic Resource
    Synaptonemal complex pairing and metaphase I association in a telo-substituted telotrisomic of rye (Secale cereale L.) (1988)
    Springer
    Chromosoma 97 (1988), S. 88-95 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The synaptonemal complexes in pollen mother cells (PMCs) of rye in which one chromosome 1R was replaced by the two corresponding telocentrics, and where one additional telocentric 1RS was present, showed approximately the expected 2∶1 ratio of 1R-1RL-1RS trivalent with 1RS univalent versus heteromorphic 1R-1RL bivalent with 1RS bivalent. In addition, however, many cells with a partner exchange were found, several even including bivalents other than 1R. At metaphase I1R-1RL-1RS trivalents predominated, cells with two univalent telocentrics were relatively frequent but partner exchange configurations were extremely rare. It is concluded that the almost consistent failure to form chiasmata in the interstitial region of 1RS after partner exchange, combined with much more frequent chiasma formation in the terminal segment, is the main reason for the unexpected metaphase I configuration frequencies. Possible causes are discussed. The shift observed does not yet explain the erratic variation in relative frequencies of metaphase I configurations reported earlier in similar material. Frequent pairing partner exchange may play a role there also.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331798
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  • 2
    Electronic Resource
    Electronic Resource
    Development and molecular cytogenetic analysis of wheat-Haynaldia villosa 6VS/6AL translocation lines specifying resistance to powdery mildew (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 1125-1128 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Haynaldia villosa ; Alien translocation ; Powdery mildew resistance ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several Triticum aestivum L.-Haynaldia villosa disomic 6VS/6AL translocation lines with powdery mildew resistance were developed from the hybridization between common wheat cultivar Yangmai 5 and alien substitution line 6V(6A). Mitotic and meiotic C-banding analysis, aneuploid analysis with double ditelosomic stocks, in situ hybridization, as well as the phenotypic assessment of powdery mildew resistance, were used to characterize these lines. The same translocated chromosome, with breakpoints near the centromere, appears to be present in all the lines, despite variation among the lines in their morphology and agronomic characteristics. The resistance gene, conferred by H. villosa and designated as Pm21, is a new and promising source of powdery mildew resistance in wheat breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223930
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation, characterization and application of a species-specific repeated sequence from Haynaldia villosa (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 526-533 
    Details
    ISSN: 1432-2242
    Keywords: Haynaldia villosa ; Repeated sequence ; In situ hybridization ; Gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A species-specific repeated sequence, pHvNAU62, was cloned from Haynaldia villosa, a wheat relative of great importance. It strongly hybridized to H. villosa, but not to wheat. In situ hybridization localized this sequence to six of seven H. villosa chromosome pairs in telomeric or sub-telomeric regions. Southern hybridization to whea-H. villosa addition lines showed that chromosomes 1V through 6V gave strong signals in ladders while chromosome 7V escaped detection. In addition to H. villosa, several Triticeae species were identified for a high abundance of the pHvNAU62 repeated sequence, among which Thinopyrum bassarabicum and Leymus racemosus produced the strongest signals. Sequence analysis indicated that the cloned fragment was 292 bp long, being AT rich (61%), and showed 67% homology of pSc7235, a rye repeated sequence. Isochizomer analysis suggested that the present repeated sequence was heavily methylated at the cytosine of the CpG dimer in the genome of H. villosa.It was also demonstrated that pHvNAU62 is useful in tagging the introduced 6VS chromosome arm, which confers a resistance gene to wheat powdery mildew, in the segregating generations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221999
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular cytogenetic analysis of Leymus racemosus chromosomes added to wheat (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 1084-1091 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Leymus racemosus ; Chromosome addition lines ; C-banding ; RLFP analysis ; Homoeology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Five disomic, two double-disomic, and two ditelosomic addition lines and one disomic substitution line derived from the crosses of Triticum aestivum (2n=6x=42, AABBDD)×Leymus racemosus (2n= 4x=28, JJNN) were identified by C-banding analysis. The homoeology of the added Leymus chromosomes was determined by RFLP analysis. Four of five disomic addition lines belonged to group 2, 5, 6 and 7 chromosomes of L. racemosus; these were designated as 2Lr?1(NAU516), 5Lr?1(NAU504, NAU514), 6Lr?1 (NAU512), and 7Lr?1(NAU501). Two additional chromosomes, 1Lr?1 and 3Lr?1, were present in double-disomic addition lines 1Lr?1+5Lr?1 (NAU525) and 3Lr?1+7Lr?1(NAU524), respec-tively. In the disomic substitution line wheat chromosome 2B was replaced by L. racemosus chromosome 2Lr?1 (NAU551). Two telocentric chromosomes, 2Lr?2S (NAU509) and 7Lr?1S (NAU511), were isolated as ditelosomic addition lines. The study presented here provides the first evidence of homoeology of the added L. racemosus chromosomes with wheat chromosomes using DNA markers. Our data provide the basis for further directed chromosome engineering aimed at producing compensating wheat-L. racemosus translocation lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050666
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  • 5
    Electronic Resource
    Electronic Resource
    Identification and physical mapping of three Haynaldia villosa chromosome-6V deletion lines (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1042-1046 
    Details
    ISSN: 1432-2242
    Keywords: Key words T. aestivum ; H. villosa ; C-banding ; RFLP ; Deletion mapping ; Pm21
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Three deletion lines (del6V?2S-1, del6V? 2L-1, and del6V?2L-2) of Haynaldia villosa chromosome 6V added to wheat were identified by C-banding and characterized by RFLP analyses. The breakpoints were located at fraction lengths (FL) 0.58 in del6V?2S-1 in the short arm, and FL 0.66 in del6V?2L-1 and FL 0.64 in del6V?2L-2 in the long arm. Thirty-one Triticeae homoeologous group-6 DNA probes were used to map RFLP loci in the deletion lines and the wheat-H. villosa disomic substitution (DS) line 6V?2(6A). Nine probes failed to detect polymorphism between Chinese Spring and DS6V?2(6A). Ten of sixteen polymorphic short-arm loci were not detected in del6V?2S-1. Thus, the loci are located in the deleted distal chromosome region. Six RFLP markers were mapped in the proximal 58% of 6VS. Of 20 DNA markers specific for 6VL, six mapped in the distal 36% of the long arm, and nine mapped in the proximal 64% of 6VL. The breakpoint of the short arm of 6V?2 occurs between Xpsr106 and Xcdo270, and that of the long arm between Xpsr915 and Xmwg934. The powdery mildew resistance gene Pm21 is located on the short arm of chromosome 6V?2. Pm21 is present in del6V?2S-1, and can be further mapped in the proximal 58% of 6V?2S.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050989
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  • 6
    Electronic Resource
    Electronic Resource
    Development of a complete set of Triticum aestivum-Aegilops speltoides chromosome addition lines (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 51-58 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Aegilops speltoides ; Chromosome addition ; C-banding ; In situ hybridization ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Aegilops speltoides Tausch (2n = 2x = 14, SS) is considered as the closest living relative of the B and G genomes of polyploid wheats. A complete set of Triticum aestivum L. cv Chinese Spring-Ae. speltoides whole chromosomes and seven telosomic addition lines was established. A low pairing accession was selected for the isolation of the chromosome addition lines. Except for chromosomes 3S and 6S, which are presently only available as monosomic additions, all other lines were recovered as disomic or ditelosomic additions. The individual Ae. speltoides chromosomes isolated in the wheat background were assayed for their genetic effects on plant phenotype and cytologically characterized in terms of chromosome length, arm ratio, distribution of marker C-bands, and FISH sites using a Ae. speltoides-specific repetitive element, Gc1R-1, as a probe. The homoeology of the added Ae. speltoides chromosomes was established by using a standard set of RFLP probes. No chromosomal rearrangements relative to wheat were detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051448
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