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  • 1
    Electronic Resource
    Electronic Resource
    Comparison of shell viral culture and serology for the diagnosis of human cytomegalovirus infection in neonates and immunocompromised subjects (1992)
    Springer
    Journal of molecular medicine 70 (1992), S. 503-507 
    Details
    ISSN: 1432-1440
    Keywords: Human cytomegalovirus ; Neonates ; Acquired immunodeficiency syndrome and AIDS related complex patients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The present retrospective study compares the laboratory diagnosis of cytomegalic inclusion disease (CID) by the use of “shell vial culture” [i.e., immunoperoxidase staining of human cytomegalovirus (HCMV) early antigen in human fibroblasts 24 h postinoculation] to the results of serology (i.e. immunoglobulins IgG, IgM, and IgA HCMV antibody testing) in 21 infants with congenital or postnatally acquired HCMV infection, 5 patients with lymphoproliferative disorders, 35 human immunodeficiency virus (HIV)-seropositive patients who met the Centers for Disease Control (CDC) criteria for stages IVA and IVB of HIV infection, and 115 patients suffering from the acquired immunodeficiency syndrome, AIDS (stages IVC-IVE according to CDC criteria). HCMV infection was diagnosed by means of the shell vial culture inoculated with patient samples (e.g., urine, bronchoalveolar lavage, induced sputum, etc.) and serology in 163 (92.6%) and 65 (36.9%) patients, respectively. Viral shedding was detected by shell vial culture in 100% of the neonates, 80% of the patients suffering from lymphoproliferative disorders, 100% of the AIDS related complex (ARC) and 89.6% of the AIDS patients. In contrast, serologic testing for HCMV-specific antibodies was positive in only 28.6%, 42.9%, and 34.8% of the neonates, ARC, and AIDS patients, respectively. In lymphoma patients, serologic testing gave identical results (80%) to the shell vial culture technique. With the use of the shell vial procedure, active HCMV infection in immunocompromised subjects and neonates can be recognized more reliably than by serologic testing. Nevertheless, in a low percentage of patients (7.4%), virus isolation by the shell vial culture may fail to detect HCMV infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210232
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  • 2
    Electronic Resource
    Electronic Resource
    131-metaiodobenzylguanedine therapy of neuroblastoma in childhood (1987)
    Springer
    European journal of pediatrics 146 (1987), S. 246-250 
    Details
    ISSN: 1432-1076
    Keywords: Neuroblastoma ; 131-metaiodobenzylguanedine treatment ; Therapeutic effect ; Tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Eleven children with neuroblastoma refractory to conventional therapy or relapse of neuroblastoma were treated with 131-metaiodobenzylguanedine (MIBG). The therapeutic results and the side effects were evaluated. In one patient with disseminated bone marrow involvement complete remission was obtained. Partial remission was observed in six patients and stable disease in another. Three patients did not respond to MIBG, in two of them the tumours did not accumulate a sufficient MIBG dose. Clinical and laboratory examinations revealed an excellent tolerance of MIBG in all patients. First attempts to continue cytostasis after MIBG therapy were made. MIBG has a good therapeutic efficacy is sufficiently incorporated into the tumour cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716467
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  • 3
    Electronic Resource
    Electronic Resource
    Chlamydia trachomatis and male infertility: chlamydia-IgA antibodies in seminal plasma are C. trachomatis specific and associated with an inflammatory response (1999)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of the European Academy of Dermatology and Venereology 12 (1999), S. 0 
    Details
    ISSN: 1468-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: There is controversy over the role of asymptomatic genital tract infection by Chlamydia trachomatis, its optimal diagnosis, and its place in the etiology of male infertility.〈section xml:id="abs1-3"〉〈title type="main"〉ObjectiveComparision of direct detection of Chlamydia trachomatis in semen with the presence of chlamydia-antibodies in seminal plasma and serum, together with parameters of the spermatogram, in men of infertile relationships.〈section xml:id="abs1-4"〉〈title type="main"〉Study designProspective clinical study.〈section xml:id="abs1-5"〉〈title type="main"〉SettingUniversity hospital tertiary referral center.〈section xml:id="abs1-6"〉〈title type="main"〉Subjects and methodsTwo groups of consecutive andrological patients (n = 89 and n= 36) were investigated as follows: semen analysis, including concentration of granulocyte-elastase; detection of C. trachomatis in semen samples and first void urine by polymerase chain reaction (PCR) and antigen-ELISA (Celisa®); detection of chlamydia antibodies in serum and seminal plasma by recombinant antibody-enzyme-linked immunosorbent assay (rELISA®) and of Chlamydia trachomatis specific antibodies by the ImmunoComb®-Chlamydia-Bivalent test.〈section xml:id="abs1-7"〉〈title type="main"〉ResultsIn 2/125 (1.6%) semen samples Chlamydia trachomatis DNA was detected by PCR. Genus specific anti-chlamydia-IgA was found in 12/122 (9%) of the seminal plasmas. This IgA appeared to be specific for C. trachomatis. Seminal plasmas with chlamydia-IgA antibodies showed higher PMN-elastase levels than IgA negative samples (P 〈 0.04). Chla-mydia-IgG antibodies were present in 27/89 (30%) of the sera, but in only five of these 27 sera (19%) were the antibodies detected specific for C. trachomatis. There were no associations between any of these variables and the parameters of the routine semen analysis.〈section xml:id="abs1-8"〉〈title type="main"〉ConclusionIgA-chlamydial antibodies in seminal plasma appeared to be specific against C. trachomatis and were associated with an inflammatory response in the male genital tract.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1468-3083.1999.tb01005.x
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  • 4
    Electronic Resource
    Electronic Resource
    Protein-free culture of VERO cells: a substrate for replication of human pathogenic viruses
    Amsterdam : Elsevier
    Cell Biology International 17 (1993), S. 885-896 
    Details
    ISSN: 1065-6995
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/cbir.1993.1152
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  • 5
    Electronic Resource
    Electronic Resource
    Aphidicolin induces myogenic differentiation in the human rhabdomyosarcoma cell line KFR.
    Amsterdam : Elsevier
    Cell Biology International 18 (1994), S. 271-278 
    Details
    ISSN: 1065-6995
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/cbir.1994.1072
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  • 6
    Electronic Resource
    Electronic Resource
    Book review (1996)
    Springer
    Infection 24 (1996), S. 360-360 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01716079
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  • 7
    Electronic Resource
    Electronic Resource
    Replication of human cytomegalovirus in a rhabdomyosarcoma cell line depends on the state of differentiation of the cells (1994)
    Springer
    Archives of virology 138 (1994), S. 391-401 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The replication of human cytomegalovirus (HCMV) was investigated in a new human rhabdomyosarcoma cell line (KFR) with morphological and biochemical characteristics of fetal striated muscle precursors (rhabdomyoblasts). KFR cells exhibited the unique property for spontaneous morphological transformation from a poorly-differentiated state into well-differentiated (myotube-like) rhabdomyoblasts. The poorly-differentiated rhabdomyoblasts promoted both complete viral gene expression and the production of infectious virus. In contrast, in well-differentiated rhabdomyoblasts HCMV infection was abortive. The results showed that replication of HCMV in this human rhabdomyosarcoma cell line depended on the state of cellular differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01379143
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  • 8
    Electronic Resource
    Electronic Resource
    Increased HIV-1 production in chronically infected H9 cells grown in protein-free medium (1992)
    Springer
    Archives of virology 125 (1992), S. 327-330 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Human T cell line H9 was established in a protein-free 1:1 mixture of Ham's F-12 and IMDM. After 230 passages (3 years) in protein-free medium, the cells designated H9-PF were infected with HIV-1. The infectivity titers of HIV-1 in cell culture medium were monitored by determining the median tissue culture infectious doses (TCID50). Additionaly, the production of viral antigen in cells was measured by an immunoenzymatical alkaline phosphatase antialkaline phosphatase (APAAP) method using a monoclonal antibody against HIV-1-p24 antigen. In acutely infected H9-PF and H9 cultures similar TCID50 values and percentage of cells positive for p24 antigen were found. In contrast, both TCID50 values and percentage of cells positive for p24 antigen were by far greater in chronically infected H9-PF than in H9 cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01309650
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  • 9
    Electronic Resource
    Electronic Resource
    Übertragbare spongiforme Encephalopathien: die Creutzfeldt-Jakob-Krankheit (1998)
    Springer
    Der Chirurg 69 (1998), S. 511-521 
    Details
    ISSN: 1433-0385
    Keywords: Key words: Transmissible spongiform encephalopathies (TSE) ; Bovine spongiform encephalopathy (BSE) ; New variant Creutzfeldt-Jakob disease (nvCJD) ; Human TSE ; epidemiology ; pathogenesis ; etiology. ; Schlüsselwörter: TSE ; BSE ; Creutzfeldt-Jakob-Krankheit ; neue Variante ; menschliche TSE (Epidemiologie ; Pathogenese ; Ätiologie).
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. Verschiedene Krankheiten aus dem Kreis der sog. übertragbaren („transmissible“) spongiformen Encephalopathien (TSE) sind beim Menschen und im Tierreich bekannt. Doch erst in jüngster Zeit sind die TSE durch die BSE-Epidemie (BSE = bovine spongiforme Encephalopathie) und die Beschreibung der wahrscheinlich damit zusammenhängenden neuen Variante der Creutzfeldt-Jakob-Krankheit (nvCJK) ins Bewußtsein der (Fach-)Öffentlichkeit gerückt. Über die Natur der zugrundeliegenden Erreger wird nach wie vor gestritten; keines der vorgeschlagenen Konzepte (Prionen, Viren) vermag alle Aspekte befriedigend zu erklären. Fest steht jedoch eine genetische Komponente bei Infektionsempfänglichkeit und Krankheitsentwicklung sowie die Übertragbarkeit auch über Artschranken hinweg. Diese Arbeit gibt einen Überblick über erste Ergebnisse der in letzter Zeit intensiver betriebenen Grundlagenforschung sowie über jüngste Entwicklungen, sowohl was den Stand der (Früh-)Diagnostik in vivo anbelangt als auch den Ausschluß von möglichen (auch iatrogenen) Übertragungswegen.
    Notes: Summary. Different diseases of the transmissible spongiform encephalopathy (TSE) group are known to affect humans and various animals. Owing to the bovine spongiform encephalopathy (BSE) epidemic and the description of the new variant of Creutzfeldt-Jakob disease (nvCJD), which is probably linked to BSE, TSE received much attention. The nature of the causative agent is still disputed; none of the proposed concepts (prions, viruses) can explain all features. It is clear, however, that there is a genetic component in susceptibility to infection and in development of disease and that transmission may cross the species barrier. This paper gives an overview of the first results and latest developments of basic TSE research that has focused on in vivo early diagnosis and the prevention of possible (also iatrogenic) transmission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001040050448
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  • 10
    Electronic Resource
    Electronic Resource
    Congenital rubella syndrome after maternal reinfection (1993)
    Springer
    Infection 21 (1993), S. 118-121 
    Details
    ISSN: 1439-0973
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Wir berichten über eine kongenitale Rötelninfektion mit atypischer Immunreaktion bei einem Kind, bei dessen Mutter eine Rötelnimmunität wiederholt vor der Schwangerschaft nachgewiesen worden war. Die Labordiagnose war ab dem 2. Lebensmonat nur eindeutig anhand der Virusisolierung möglich, obwohl das klassische klinische Erscheinungsbild eines kongenitalen Rötelnsyndroms vorlag und mehrere Organe involviert waren. Nach dem ersten Lebensmonat wurden vorübergehend spezifische IgM-Antikörper mit 2 ELISA-Tests in schwacher Konzentration nachgewiesen und mit einem Referenzverfahren (Inhibierung der Hämagglutination mit der isolierten IgM-Fraktion) bestätigt. Persistierende IgM-Antikörper in ansteigender Konzentration waren ab dem 6. Lebensmonat nachweisbar. Im weiteren Verlauf sanken die spezifischen IgG-Antikörper ab. Die immunologische Analyse wies eine IgG1-Hypoglobulinämie nach. Die Besonderheit des geschilderten Falles liegt nicht nur im Versagen der mütterlichen Immunität, eine kongenitale Rötelninfektion zu verhindern, sondern auch im Defekt des kindlichen Immunsystems, welcher möglicherweise durch die kongenitale Rötelninfektion bedingt war, so daß über die bewährten Methoden der Infektionsserologie die Labordiagnose zunächst nicht gestellt werden konnte.
    Notes: Summary This report concerns a case of congenital rubella syndrome (CRS) with atypical immune response affecting an infant whose mother had repeated evidence of immunity before pregnancy. Laboratory diagnosis of CRS could only clearly be achieved by virus isolation after the second month of life despite typical clinical features of CRS and multiple organ involvement. After the first month of age, low concentrations of specific IgM antibodies were revealed by ELISA and confirmed by a reference test system (IgM-specific haemagglutination inhibition assay). Persistent and increasing high levels of IgM antibodies were detected only after the 6th month of life. Later on IgG antibody levels decreased. Immunological investigations showed an IgG1-hypoglobulinaemia. The unusual feature of the present case report is not only the failure of the maternal rubella immunity to prevent CRS, but the defect of the child's immune system, probably attributable to congenital infection. As a consequence, laboratory diagnosis of CRS could not be achieved initially by the proved serological methods.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01710747
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