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  • 1
    Electronic Resource
    Electronic Resource
    Regeneration of transgenic plants from the commercial apple cultivar Royal Gala (1995)
    Springer
    Plant cell reports 14 (1995), S. 407-412 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A transformation system was developed for the commercial apple (Malus X domestica Borkh.) cultivar Royal Gala. Leaf pieces from in vitro-grown shoots were cocultivated for 2 days with Agrobacterium tumefaciens strain LBA4404 containing the binary vectors pKIWI105 or pKIWI110. Shoots were produced on a shooting medium containing kanamycin (50 mg·L−1). A 2-day incubation period on kanamycin-free medium prior to antibiotic selection enhanced the regeneration of kanamycin-resistant shoots. The majority of the kanamycin-resistant shoots also expressed GUS (β-glucuronidase) activity. The putatively transformed shoots were rooted on a medium containing kanamycin (50 mg·L−1). Rooted plants were established in a greenhouse, and plants transformed with pKIWI110, which contains a mutant Arabidopsis acetolactate synthase gene, were shown to be resistant to the herbicide Glean™. Integration of T-DNA into the apple genome was confirmed by PCR and Southern hybridization analyses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234044
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of pollination-induced genes from the ovary of apple (Malus domestica) (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 277-283 
    Details
    ISSN: 1432-2145
    Keywords: Key words Chitinase ; Defence ; Differential hybridization ; Fruit development ; Gibberellin ; Histone H2B
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A set of fifteen cDNA clones from apple (Malus domestica Borkh) corresponding to fruit genes induced or enhanced by pollination have been identified by differential hybridization. Expression of corresponding mRNAs was induced in apple flowers by pollination, and in six clones mRNA levels also showed induction by gibberellin treatment of flowers. Sequence analysis and database searches showed that these cDNAs correspond to genes involved in defence responses, transport, protein and flavonoid synthesis, as well as cell division. One of the pollination-enhanced cDNAs was found to be similar to plant and animal genes encoding histone H2B. This mRNA was very highly expressed in flower buds and in fruit at early stages of development, but transcript levels were relatively low in young leaves and shoot tips. RNA in situ hybridization showed histone H2B mRNA detectable at high levels in the nucellus tissue of ovules in unopened flower buds. Five days after pollination, transcript levels decreased in the nucellus; however, weak signals were observed in the fleshy cortex tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050154
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    MDH1: an apple homeobox gene belonging to the BEL1 family (2000)
    Springer
    Plant molecular biology 42 (2000), S. 623-633 
    Details
    ISSN: 1573-5028
    Keywords: cell morphology ; homeobox ; Malus domestica ; fertility ; fruit development ; ovule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Differential display was used to isolate genes differentially expressed early in fruit development of apple (Malus domestica Borkh.). This approach resulted in the isolation of MDH1, a homeobox gene with a homeodomain similar to that of BELL1 (BEL1), which is involved in regulation of ovule development in Arabidopsis. However, outside the homeodomain MDH1 is quite different from BEL1. In apple, MDH1 mRNA was predominantly found in flowers, expanding leaves and expanding fruit. In pre-anthesis flowers, in situ hybridization showed that MDH1 mRNA accumulated in ovules. To further investigate the function of this new homeobox gene, MDH1 was transformed into Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter. The transgenic Arabidopsis plants showed dwarfing, reduced fertility and changes in carpel and fruit (silique) shape. The size and shape of the cells in the transgenic fruit was irregular. Both the transgenic phenotypes in Arabidopsis and the expression pattern of this gene in apple are consistent with the idea that MDH1 is likely to play an important role in control of plant fertility.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006301224125
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro chromosome doubling of nineZantedeschia cultivars (1996)
    Springer
    Plant cell, tissue and organ culture 47 (1996), S. 43-49 
    Details
    ISSN: 1573-5044
    Keywords: calla lily ; colchicine ; stomatal length ; tetraploid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tetraploid plants have been produced from nineZantedeschia cultivars usingin vitro colchicine treatment. Rapidly-multiplying shoot cultures were treated on a medium containing 0.05% colchicine for 1, 2 or 4 days to induce chromosome doubling. Following the treatment, most shoots were killed but the surviving shoots were multiplied for several subcultures. These shoots were then rootedin vitro and transferred to a greenhouse. Plants were screened 2 months later by measuring stomatal length, and 110 out of 565 plants were selected as putative tetraploids with a stomatal length significantly greater than in diploid control plants. Chromosome counts were carried out on root tips from 44 plants and confirmed that 38 were tetraploids, 2 were chimeras (predominantly tetraploid with a few octoploid cells), and 4 were diploids. Stomatal length has been rechecked in mature tetraploid plants of the cultivar Black Magic, demonstrating that stomatal length is a good indicator of ploidy level inZantedeschia. This study has shown that multiplying colchicine-treated shootsin vitro for several subcultures prior to transfer to soil produced very few chimeras. The stomatal length measurements are non-destructive and allow the rapid screening of a population for tetraploids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02318964
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    Paper (German National Licenses)
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